Restriction map for
pFN21AB4489
(Circular) (Six-base) MAPSORT of: pFN21AB4489.seq Check: 1540 from: 1 to: 6106
pFN21AB4489 6106 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 6106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 3381 4169 5553 5821 1632
Size: 713 1036 788 1384 268 1917
Fragments arranged by size:
1917 1384 1036 788 713 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 2589 2758 5005 5982 1203
Size: 1386 169 2247 977 1327
Fragments arranged by size:
2247 1386 1327 977 169
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 3343 1166
Size: 2177 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 3089 1184
Size: 1095 810 4201
Fragments arranged by size:
4201 1095 810
AlwNI CAG_nnn'CTG
Cuts at: 2937 3141 3184 3349 3404 3849 5421 2937
Size: 204 43 165 55 445 1572 3622
Fragments arranged by size:
3622 1572 445 204 165 55 43
ApaLI G'TGCA_C
Cuts at: 2608 3011 4163 5319 2608
Size: 403 1152 1156 3395
Fragments arranged by size:
3395 1156 1152 403
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2188 3431 1870
Size: 73 245 1243 4545
Fragments arranged by size:
4545 1243 245 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3427 4882 618
Size: 325 217 627 1640 1455 1842
Fragments arranged by size:
1842 1640 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2179 2814 3377 3425 1391
Size: 788 635 563 48 4072
Fragments arranged by size:
4072 788 635 563 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3148 928
Size: 971 1249 3886
Fragments arranged by size:
3886 1249 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4297 4331 1903
Size: 34 50 34 2276 34 3678
Fragments arranged by size:
3678 2276 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 2465 3177 3523 2465
Size: 712 346 5048
Fragments arranged by size:
5048 712 346
Bme1580I G_kGCm'C
Cuts at: 1790 2612 3015 4167 5323 1790
Size: 822 403 1152 1156 2573
Fragments arranged by size:
2573 1156 1152 822 403
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 3184 3837
Size: 416 53 83 399 24 1176 591 653
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1176 958 653 591 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2354 2397 4766 1239
Size: 950 165 43 2369 2579
Fragments arranged by size:
2579 2369 950 165 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3134 4231 5096 5394 3134
Size: 1097 865 298 3846
Fragments arranged by size:
3846 1097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 4769 882
Size: 617 1212 2058 2219
Fragments arranged by size:
2219 2058 1212 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3246 4539 4937 5211 5358 5769 3246
Size: 1293 398 274 147 411 3583
Fragments arranged by size:
3583 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 2612 2642 1178
Size: 30 165 30 1176 30 3 30 4642
Fragments arranged by size:
4642 1176 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2822 1186
Size: 1636 4470
BseYI C'CCAG_C
Cuts at: 2198 2460 2582 2814 3112 3134 5309 5805 2198
Size: 262 122 232 298 22 2175 496 2499
Fragments arranged by size:
2499 2175 496 298 262 232 122 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 2550 3095 1163
Size: 573 747 67 545 4174
Fragments arranged by size:
4174 747 573 545 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2832 3556 4317 5345 1540
Size: 275 186 831 724 761 1028 2301
Fragments arranged by size:
2301 1028 831 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 2564 2612 2828 2895 3015 4167 4252 5323
Size: 131 48 216 67 120 1152 85 1071
Cuts at: 5323 2433
Size: 3216
Fragments arranged by size:
3216 1152 1071 216 131 120 85 67
48
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2433 2564 2612 2814 2828 2895
Size: 399 389 254 131 48 202 14 67
Cuts at: 2895 3015 3377 3425 4167 4252 5323 1391
Size: 120 362 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 389 362 254 202
131 120 85 67 48 48 14
BspEI T'CCGG_A
Cuts at: 3246 5769 3246
Size: 2523 3583
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 3461 844
Size: 1389 1228 3489
Fragments arranged by size:
3489 1389 1228
BsrBI CCG'CTC
Cuts at: 1394 1823 2223 3993 1394
Size: 429 400 1770 3507
Fragments arranged by size:
3507 1770 429 400
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 2318 2432 4166 5178 2318
Size: 114 1734 1012 3246
Fragments arranged by size:
3246 1734 1012 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3309 794
Size: 2515 3591
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 3345 3345
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2302 3129 3436 3803 4188 4205 5657 5743
Size: 330 827 307 367 385 17 1452 86
Cuts at: 5743 5755 6101 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 827 385 367 346 330 307
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2157 3202 513
Size: 552 1092 1045 3417
Fragments arranged by size:
3417 1092 1045 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3269 487
Size: 683 56 2043 3324
Fragments arranged by size:
3324 2043 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2121 3338 3350 3622 4416 4436 2061
Size: 60 1217 12 272 794 20 3731
Fragments arranged by size:
3731 1217 794 272 60 20 12
ClaI AT'CG_AT
Cuts at: 1554 2771 1554
Size: 1217 4889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2297 2475 2613
Size: 54 1425 50 275 18 467 178 138
Cuts at: 2613 2728 3553 4442 6071 8
Size: 115 825 889 1629 43
Fragments arranged by size:
1629 1425 889 825 467 275 178 138
115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2054 2085 3176 4036 1353
Size: 686 15 31 1091 860 3423
Fragments arranged by size:
3423 1091 860 686 31 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2345 2354 2397 3381 4169 4766
Size: 393 557 156 9 43 984 788 597
Cuts at: 4766 5553 5821 1239
Size: 787 268 1524
Fragments arranged by size:
1524 984 788 787 597 557 393 268
156 43 9
EcoO109I rG'GnC_Cy
Cuts at: 2260 2260
Size: 6106
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2296 2493 5253 1678
Size: 618 197 2760 2531
Fragments arranged by size:
2760 2531 618 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2266 2294 2298 2326 2647
Size: 32 135 32 694 28 4 28 321
Cuts at: 2647 2679 3361 3393 4747 4779 4821 4853 1373
Size: 32 682 32 1354 32 42 32 2626
Fragments arranged by size:
2626 1354 694 682 321 135 42 32
32 32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3450 3696 669
Size: 988 281 739 773 246 3079
Fragments arranged by size:
3079 988 773 739 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 2589 5982 2589
Size: 3393 2713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 2730 10
Size: 54 1425 343 645 253 3386
Fragments arranged by size:
3386 1425 645 343 253 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3071 3330 3343 4064 4423 4582 518
Size: 648 1905 259 13 721 359 159 2042
Fragments arranged by size:
2042 1905 721 648 359 259 159 13
MspA1I CmG'CkG
Cuts at: 1606 2184 2332 2750 3138 3522 4199 5347 5592
Size: 578 148 418 388 384 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 578 418 388 384 245
148
NcoI C'CATG_G
Cuts at: 513 1065 2157 513
Size: 552 1092 4462
Fragments arranged by size:
4462 1092 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2762 3464 5009 1132
Size: 97 1533 702 1545 2229
Fragments arranged by size:
2229 1545 1533 702 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 2758 5005 2758
Size: 2247 3859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 2244 2310 3146 3345 3458 830
Size: 1414 66 836 199 113 3478
Fragments arranged by size:
3478 1414 836 199 113 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2184 2750 2184
Size: 566 5540
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 3176 3176
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 2418 4354 6066 2418
Size: 1936 1712 2458
Fragments arranged by size:
2458 1936 1712
SfcI C'TryA_G
Cuts at: 826 1045 2240 2306 2736 3142 3341 3454 4589
Size: 219 1195 66 430 406 199 113 1135
Cuts at: 4589 5270 5461 826
Size: 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 430 406 219 199
191 113 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 2190 3433 1872
Size: 318 1243 4545
Fragments arranged by size:
4545 1243 318
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3149 4210 5111 5373 820
Size: 197 926 1206 1061 901 262 1553
Fragments arranged by size:
1553 1206 1061 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2157 3545 513
Size: 552 1092 1388 3074
Fragments arranged by size:
3074 1388 1092 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 2818 3126 4133 4150 4303 4488
Size: 1255 1016 222 308 1007 17 153 185
Cuts at: 4488 4933 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1016 1007 445 308 222 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2416 3018 3239
Size: 274 80 33 51 1443 439 602 221
Cuts at: 3239 4352 6064 96
Size: 1113 1712 138
Fragments arranged by size:
1712 1443 1113 602 439 274 221 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2994 3026 6066 6098 2579
Size: 32 383 32 3040 32 2587
Fragments arranged by size:
3040 2587 383 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 2188 3431 1870
Size: 318 1243 4545
Fragments arranged by size:
4545 1243 318
XmnI GAAnn'nnTTC
Cuts at: 2641 2933 4235 2641
Size: 292 1302 4512
Fragments arranged by size:
4512 1302 292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI