Restriction map for
pFN21AB4461
(Circular) (Six-base) MAPSORT of: pFN21AB4461.seq Check: 7450 from: 1 to: 6427
pFN21AB4461 6427 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 2032
Size: 6427
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3426 4490 5874 6142 1632
Size: 1794 1064 1384 268 1917
Fragments arranged by size:
1917 1794 1384 1064 268
AfeI AGC'GCT
Cuts at: 2632 2632
Size: 6427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 2376 5326 6303 1203
Size: 1173 2950 977 1327
Fragments arranged by size:
2950 1327 1173 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 4170 5742 4170
Size: 1572 4855
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 3736 3962 4114 6223 1493
Size: 2243 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 3752 1870
Size: 73 1809 4545
Fragments arranged by size:
4545 1809 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2097 2668 3083 3748
Size: 325 217 627 234 76 571 415 665
Cuts at: 3748 5203 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 665 627 571 415 325 234
217 76
BanII G_rGCy'C
Cuts at: 1391 3746 1391
Size: 2355 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 3502 4319 5529 3338
Size: 164 817 1210 4236
Fragments arranged by size:
4236 1210 817 164
BclI T'GATC_A
Cuts at: 1548 2847 2853 2964 1548
Size: 1299 6 111 5011
Fragments arranged by size:
5011 1299 111 6
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2352 3305 5038
Size: 107 122 71 631 605 680 953 1733
Cuts at: 5038 136
Size: 1525
Fragments arranged by size:
1733 1525 953 680 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2357 3086 4488 5644 1790
Size: 567 729 1402 1156 2573
Fragments arranged by size:
2573 1402 1156 729 567
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2414 2951 3327
Size: 416 53 83 399 24 997 537 376
Cuts at: 3327 4158 5116 442
Size: 831 958 1753
Fragments arranged by size:
1753 997 958 831 537 416 399 376
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2284 2726 5087 1239
Size: 1045 442 2361 2579
Fragments arranged by size:
2579 2361 1045 442
Bpu10I CC'TnA_GC
Cuts at: 2334 2334
Size: 6427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 4552 5417 5715 2435
Size: 2117 865 298 3147
Fragments arranged by size:
3147 2117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5090 882
Size: 617 3591 2219
Fragments arranged by size:
3591 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 2690 2852 6421 2690
Size: 162 3569 2696
Fragments arranged by size:
3569 2696 162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3023 3053 1178
Size: 30 165 30 1620 30 4552
Fragments arranged by size:
4552 1620 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 2690 3347 1186
Size: 1441 63 657 4266
Fragments arranged by size:
4266 1441 657 63
BseYI C'CCAG_C
Cuts at: 2125 2461 3473 3522 5630 6126 2125
Size: 336 1012 49 2108 496 2426
Fragments arranged by size:
2426 2108 1012 496 336 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3609 1163
Size: 573 1873 3981
Fragments arranged by size:
3981 1873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4488 4573 5644 4488
Size: 85 1071 5271
Fragments arranged by size:
5271 1071 85
BsmI GAATG_Cn'
Cuts at: 2061 3656 3938 4031 2061
Size: 1595 282 93 4457
Fragments arranged by size:
4457 1595 282 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 3086 3746 4488 4573 5644 1391
Size: 399 567 729 660 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 660 567 399 85
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 2259 2850 4316 6046 2259
Size: 591 1466 1730 2640
Fragments arranged by size:
2640 1730 1466 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 3782 844
Size: 1188 1750 3489
Fragments arranged by size:
3489 1750 1188
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 2751 2904 4922 5096 65
Size: 1183 1143 360 153 2018 174 1396
Fragments arranged by size:
2018 1396 1183 1143 360 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 5071 5258 1700
Size: 110 284 2977 187 2869
Fragments arranged by size:
2977 2869 284 187 110
BsrGI T'GTAC_A
Cuts at: 96 2949 96
Size: 2853 3574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 2356 4487 5499 2356
Size: 2131 1012 3284
Fragments arranged by size:
3284 2131 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3532 794
Size: 2738 3689
BstBI TT'CG_AA
Cuts at: 2521 5231 2521
Size: 2710 3717
BstEII G'GTnAC_C
Cuts at: 1184 3346 1184
Size: 2162 4265
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2800 3196 3251 2044
Size: 756 396 55 5220
Fragments arranged by size:
5220 756 396 55
BstYI r'GATC_y
Cuts at: 1972 3757 4124 4509 4526 5978 6064 6076 6422
Size: 1785 367 385 17 1452 86 12 346
Cuts at: 6422 1972
Size: 1977
Fragments arranged by size:
1977 1785 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2364 2983 3372 513
Size: 552 939 360 619 389 3568
Fragments arranged by size:
3568 939 619 552 389 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2749 487
Size: 683 56 1523 4165
Fragments arranged by size:
4165 1523 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 3072 3700 3943 4737 4757 2184
Size: 888 628 243 794 20 3854
Fragments arranged by size:
3854 888 794 628 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 3321 3874
Size: 54 1425 50 275 18 514 977 553
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 977 889 553 514 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 4357 1353
Size: 1181 1823 3423
Fragments arranged by size:
3423 1823 1181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2284 2726 3426 4490 5087 5874 6142
Size: 393 652 442 700 1064 597 787 268
Cuts at: 6142 1239
Size: 1524
Fragments arranged by size:
1524 1064 787 700 652 597 442 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2465 2701 2465
Size: 236 6191
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2634 5574 1678
Size: 956 2940 2531
Fragments arranged by size:
2940 2531 956
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2770 2802 3469
Size: 32 135 32 902 32 264 32 667
Cuts at: 3469 3501 3544 3576 5068 5100 5142 5174 1373
Size: 32 43 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 902 667 264 135 43 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3063 748
Size: 2315 4112
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3160 5541 5725 1305
Size: 1855 2381 184 2007
Fragments arranged by size:
2381 2007 1855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4605
Fragments arranged by size:
4605 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2220 2264 2768 2855 3194 4385 4744
Size: 648 1054 44 504 87 339 1191 359
Cuts at: 4744 4903 518
Size: 159 2042
Fragments arranged by size:
2042 1191 1054 648 504 359 339 159
87 44
MspA1I CmG'CkG
Cuts at: 1606 2461 3843 4520 5668 5913 1606
Size: 855 1382 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 855 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 4576
Fragments arranged by size:
4576 939 552 360
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2380 3785 5330 1132
Size: 97 1151 1405 1545 2229
Fragments arranged by size:
2229 1545 1405 1151 97
PasI CC'CwG_GG
Cuts at: 1348 2246 2872 3079 1348
Size: 898 626 207 4696
Fragments arranged by size:
4696 898 626 207
PciI A'CATG_T
Cuts at: 2376 5326 2376
Size: 2950 3477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6427
PfoI T'CCnGG_A
Cuts at: 2508 3261 2508
Size: 753 5674
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2401 3695 3779 830
Size: 1571 1294 84 3478
Fragments arranged by size:
3478 1571 1294 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 2403 4675 6387 2403
Size: 2272 1712 2443
Fragments arranged by size:
2443 2272 1712
SfcI C'TryA_G
Cuts at: 826 1045 2397 3691 3775 4910 5591 5782 826
Size: 219 1352 1294 84 1135 681 191 1471
Fragments arranged by size:
1471 1352 1294 1135 681 219 191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 4531 5432 5694 820
Size: 197 926 507 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 507 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 2325 2973 1517
Size: 808 648 4971
Fragments arranged by size:
4971 808 648
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 3710 3866 513
Size: 552 939 360 1346 156 3074
Fragments arranged by size:
3074 1346 939 552 360 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3446 4454 4471 4624 4809 5254 325
Size: 1255 1866 1008 17 153 185 445 1498
Fragments arranged by size:
1866 1498 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2401 2949 4673
Size: 274 80 33 51 1443 424 548 1724
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
1724 1712 1443 548 424 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3264 3296 6387 6419 3264
Size: 32 3091 32 3272
Fragments arranged by size:
3272 3091 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2800 3340 3502 2800
Size: 540 162 5725
Fragments arranged by size:
5725 540 162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI