Restriction map for
pFN21AB4458
(Circular) (Six-base) MAPSORT of: pFN21AB4458.seq Check: 8377 from: 1 to: 8335
pFN21AB4458 8335 bp
With 182 enzymes: SgfI *
March 2, 2009 15:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2801 2882 3722 2801
Size: 81 840 7414
Fragments arranged by size:
7414 840 81
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6145 278
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4833 5678 1937
Size: 2896 845 4594
Fragments arranged by size:
4594 2896 845
Acc65I G'GTAC_C
Cuts at: 5656 5656
Size: 8335
AclI AA'CG_TT
Cuts at: 1563 6462 6835 1563
Size: 4899 373 3063
Fragments arranged by size:
4899 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 5059 6398 7782 8050 1632
Size: 788 2639 1339 1384 268 1917
Fragments arranged by size:
2639 1917 1384 1339 788 268
AfeI AGC'GCT
Cuts at: 2034 4934 2034
Size: 2900 5435
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8138
AflIII A'CryG_T
Cuts at: 1203 2529 4323 7234 8211 1203
Size: 1326 1794 2911 977 1327
Fragments arranged by size:
2911 1794 1327 1326 977
AgeI A'CCGG_T
Cuts at: 7166 7166
Size: 8335
AhdI GACnn_n'nnGTC
Cuts at: 3456 7064 3456
Size: 3608 4727
AleI CACnn'nnGTG
Cuts at: 1166 2876 4686 5399 1166
Size: 1710 1810 713 4102
Fragments arranged by size:
4102 1810 1710 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 8303
AlwNI CAG_nnn'CTG
Cuts at: 2632 2694 3178 4444 5273 5347 6078 7650 2632
Size: 62 484 1266 829 74 731 1572 3317
Fragments arranged by size:
3317 1572 1266 829 731 484 74 62
ApaI G_GGCC'C
Cuts at: 3557 3964 5479 3557
Size: 407 1515 6413
Fragments arranged by size:
6413 1515 407
ApaLI G'TGCA_C
Cuts at: 4505 6392 7548 4505
Size: 1887 1156 5292
Fragments arranged by size:
5292 1887 1156
ApoI r'AATT_y
Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 4136
Size: 701 104 49 72 343 273 371 730
Cuts at: 4136 4221 4904 5621 5644 5870 6022 8131 1493
Size: 85 683 717 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 730 717 701 683 371 343
273 226 152 104 85 72 49 23
AseI AT'TA_AT
Cuts at: 160 1032 6889 160
Size: 872 5857 1606
Fragments arranged by size:
5857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
AvaI C'yCGr_G
Cuts at: 1870 1943 2733 2846 3539 4920 5411 5660 1870
Size: 73 790 113 693 1381 491 249 4545
Fragments arranged by size:
4545 1381 790 693 491 249 113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3724 3757 3724
Size: 33 8302
BamHI G'GATC_C
Cuts at: 3166 4197 4736 5665 6032 3166
Size: 1031 539 929 367 5469
Fragments arranged by size:
5469 1031 929 539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2867 4014 5228 5656
Size: 325 217 627 654 426 1147 1214 428
Cuts at: 5656 7111 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1214 1147 654 627 428 426
325 217
BanII G_rGCy'C
Cuts at: 1391 2077 3557 3564 3964 4163 5114 5479 5573
Size: 686 1480 7 400 199 951 365 94
Cuts at: 5573 5654 1391
Size: 81 4072
Fragments arranged by size:
4072 1480 951 686 400 365 199 94
81 7
BbeI G_GCGC'C
Cuts at: 2445 4018 5232 2445
Size: 1573 1214 5548
Fragments arranged by size:
5548 1573 1214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 5025 5196 928
Size: 971 1071 2055 171 4067
Fragments arranged by size:
4067 2055 1071 971 171
BbvCI CC'TCA_GC
Cuts at: 4806 4806
Size: 8335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5447 5481 6526 6560 1903
Size: 34 50 34 3426 34 1045 34 3678
Fragments arranged by size:
3678 3426 1045 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 6227 7437 3752
Size: 2475 1210 4650
Fragments arranged by size:
4650 2475 1210
BclI T'GATC_A
Cuts at: 1548 4839 4896 1548
Size: 3291 57 4987
Fragments arranged by size:
4987 3291 57
BfrBI ATG'CAT
Cuts at: 4966 4966
Size: 8335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 4338 5474
Size: 107 122 71 631 605 1267 1399 1136
Cuts at: 5474 6946 136
Size: 1472 1525
Fragments arranged by size:
1525 1472 1399 1267 1136 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4770 8330 4770
Size: 3560 4775
BlpI GC'TnA_GC
Cuts at: 5752 5752
Size: 8335
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2653 3557 3754 3964 4509 5005 5479
Size: 342 521 904 197 210 545 496 474
Cuts at: 5479 6396 7552 1790
Size: 917 1156 2573
Fragments arranged by size:
2573 1156 917 904 545 521 496 474
342 210 197
BmgBI CAC'GTC
Cuts at: 1318 5032 5091 1318
Size: 3714 59 4562
Fragments arranged by size:
4562 3714 59
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 3957 4080
Size: 416 53 83 399 24 1265 1275 123
Cuts at: 4080 4432 5261 5482 6066 7024 442
Size: 352 829 221 584 958 1753
Fragments arranged by size:
1753 1275 1265 958 829 584 416 399
352 221 123 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 4792 4817 2091
Size: 2701 25 5609
Fragments arranged by size:
5609 2701 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2141 3650 4717 4805 6995 1239
Size: 860 42 1509 1067 88 2190 2579
Fragments arranged by size:
2579 2190 1509 1067 860 88 42
Bpu10I CC'TnA_GC
Cuts at: 2359 4806 5108 2359
Size: 2447 302 5586
Fragments arranged by size:
5586 2447 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 4135 6460 7325 7623 3347
Size: 788 2325 865 298 4059
Fragments arranged by size:
4059 2325 865 788 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4917 5361 6998 882
Size: 617 3418 444 1637 2219
Fragments arranged by size:
3418 2219 1637 617 444
BsaAI yAC'GTr
Cuts at: 493 1204 2781 2826 4297 4324 6126 493
Size: 711 1577 45 1471 27 1802 2702
Fragments arranged by size:
2702 1802 1577 1471 711 45 27
BsaBI GATnn'nnATC
Cuts at: 8329 8329
Size: 8335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2970 3840 4015
Size: 53 83 186 936 909 528 870 175
Cuts at: 4015 5229 6142 6524 7159 275
Size: 1214 913 382 635 1451
Fragments arranged by size:
1451 1214 936 913 909 870 635 528
382 186 175 83 53
BsaWI w'CCGG_w
Cuts at: 2393 3455 4163 6768 7166 7440 7587 7998 2393
Size: 1062 708 2605 398 274 147 411 2730
Fragments arranged by size:
2730 2605 1062 708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139
Size: 30 165 30 1632 30 12 30 32
Cuts at: 3139 3169 4614 4644 1178
Size: 30 1445 30 4869
Fragments arranged by size:
4869 1632 1445 165 32 30 30 30
30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 3147 3150 3956 4765 1186
Size: 1444 517 3 806 809 4756
Fragments arranged by size:
4756 1444 809 806 517 3
BseYI C'CCAG_C
Cuts at: 2601 2652 3549 4156 4336 4588 4929 5360 7538
Size: 51 897 607 180 252 341 431 2178
Cuts at: 7538 8034 2601
Size: 496 2902
Fragments arranged by size:
2902 2178 897 607 496 431 341 252
180 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 4481
Size: 573 498 394 79 366 627 159 622
Cuts at: 4481 5339 1163
Size: 858 4159
Fragments arranged by size:
4159 858 627 622 573 498 394 366
159 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 5785 6546 7574 1540
Size: 275 186 24 3760 761 1028 2301
Fragments arranged by size:
3760 2301 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2663 3536 4509 5114 6396 6481 7552 2663
Size: 873 973 605 1282 85 1071 3446
Fragments arranged by size:
3446 1282 1071 973 873 605 85
BsmI GAATG_Cn'
Cuts at: 3611 4968 5846 5939 3611
Size: 1357 878 93 6007
Fragments arranged by size:
6007 1357 878 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2763 3580 2763
Size: 817 7518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564
Size: 399 287 55 521 10 873 21 7
Cuts at: 3564 3754 3964 4163 4509 5005 5114 5479 5573
Size: 190 210 199 346 496 109 365 94
Cuts at: 5573 5654 6396 6481 7552 1391
Size: 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 521 496 399 365
346 287 210 199 190 109 94 85
81 55 21 10 7
BspEI T'CCGG_A
Cuts at: 7998 7998
Size: 8335
BspHI T'CATG_A
Cuts at: 6224 7954 6224
Size: 1730 6605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 2801 2882 3722 3794 4452 5690 844
Size: 1478 479 81 840 72 658 1238 3489
Fragments arranged by size:
3489 1478 1238 840 658 479 81 72
BsrBI CCG'CTC
Cuts at: 1394 1823 6222 1394
Size: 429 4399 3507
Fragments arranged by size:
4399 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 6830 7004 65
Size: 1183 1026 4556 174 1396
Fragments arranged by size:
4556 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3121 3842 6979 7166 1700
Size: 110 1311 721 3137 187 2869
Fragments arranged by size:
3137 2869 1311 721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8335
BssSI C'ACGA_G
Cuts at: 2104 2920 6395 7407 2104
Size: 816 3475 1012 3032
Fragments arranged by size:
3475 3032 1012 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3606 3675 4381 794
Size: 2812 69 706 4748
Fragments arranged by size:
4748 2812 706 69
BstBI TT'CG_AA
Cuts at: 7139 7139
Size: 8335
BstEII G'GTnAC_C
Cuts at: 1184 4708 1184
Size: 3524 4811
BstXI CCAn_nnnn'nTGG
Cuts at: 3952 4588 3952
Size: 636 7699
BstYI r'GATC_y
Cuts at: 1972 3166 3930 4119 4197 4736 4770 5665 6032
Size: 1194 764 189 78 539 34 895 367
Cuts at: 6032 6417 6434 7886 7972 7984 8330 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1194 895 764 539 385 367
346 189 86 78 34 17 12
Bsu36I CC'TnA_GG
Cuts at: 3696 5597 3696
Size: 1901 6434
BtgI C'CryG_G
Cuts at: 513 1065 2674 5142 513
Size: 552 1609 2468 3706
Fragments arranged by size:
3706 2468 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3925 487
Size: 683 56 2699 4897
Fragments arranged by size:
4897 2699 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2663 3772 5394 5406 5623 5851 6645 6665
Size: 145 1109 1622 12 217 228 794 20
Cuts at: 6665 2518
Size: 4188
Fragments arranged by size:
4188 1622 1109 794 228 217 145 20
12
ClaI AT'CG_AT
Cuts at: 1554 4827 1554
Size: 3273 5062
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8300
DraI TTT'AAA
Cuts at: 3040 5639 3040
Size: 2599 5736
DraIII CAC_nnn'GTG
Cuts at: 4088 4088
Size: 8335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 7342 809
Size: 507 1434 4592 1802
Fragments arranged by size:
4592 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4302
Size: 54 1425 50 275 18 192 1822 458
Cuts at: 4302 4578 5782 6671 8300 8
Size: 276 1204 889 1629 43
Fragments arranged by size:
1822 1629 1425 1204 889 458 276 275
192 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 5782 1537
Size: 275 210 3760 4090
Fragments arranged by size:
4090 3760 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 4007 4593 6265 1353
Size: 2654 586 1672 3423
Fragments arranged by size:
3423 2654 1672 586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6922 7296 7442 387
Size: 1064 138 5333 374 146 1280
Fragments arranged by size:
5333 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5112 5112
Size: 8335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2141 2420 3650 4717 4805 5059
Size: 393 467 42 279 1230 1067 88 254
Cuts at: 5059 6398 6995 7782 8050 1239
Size: 1339 597 787 268 1524
Fragments arranged by size:
1524 1339 1230 1067 787 597 467 393
279 268 254 88 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3629 3695 3629
Size: 66 8269
EcoO109I rG'GnC_Cy
Cuts at: 2729 2996 3450 3553 3960 3961 5383 2729
Size: 267 454 103 407 1 1422 5681
Fragments arranged by size:
5681 1422 454 407 267 103 1
EcoRI G'AATT_C
Cuts at: 2298 4136 4904 5644 6022 2298
Size: 1838 768 740 378 4611
Fragments arranged by size:
4611 1838 768 740 378
FspI TGC'GCA
Cuts at: 6841 6841
Size: 8335
HaeII r_GCGC'y
Cuts at: 1678 2036 2045 2445 2671 4018 4936 5232 7482
Size: 358 9 400 226 1347 918 296 2250
Cuts at: 7482 1678
Size: 2531
Fragments arranged by size:
2531 2250 1347 918 400 358 296 226
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442
Size: 32 135 32 1331 32 100 32 375
Cuts at: 3442 3474 3560 3592 4182 4214 6976 7008 7050
Size: 32 86 32 590 32 2762 32 42
Cuts at: 7050 7082 1373
Size: 32 2626
Fragments arranged by size:
2762 2626 1331 590 375 135 100 86
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5446 5679 5925 669
Size: 988 281 3508 233 246 3079
Fragments arranged by size:
3508 3079 988 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8335
HpaI GTT'AAC
Cuts at: 5925 5925
Size: 8335
KasI G'GCGC_C
Cuts at: 2441 4014 5228 2441
Size: 1573 1214 5548
Fragments arranged by size:
5548 1573 1214
KpnI G_GTAC'C
Cuts at: 5660 5660
Size: 8335
MfeI C'AATT_G
Cuts at: 5934 5934
Size: 8335
MluI A'CGCG_T
Cuts at: 8211 8211
Size: 8335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3938 5013 7449 7633 1305
Size: 2633 1075 2436 184 2007
Fragments arranged by size:
2633 2436 2007 1075 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4304 4580 10
Size: 54 1425 343 2472 276 3765
Fragments arranged by size:
3765 2472 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2876 3950 4686 4816 4841 5399
Size: 648 1368 342 1074 736 130 25 558
Cuts at: 5399 6293 6652 6811 518
Size: 894 359 159 2042
Fragments arranged by size:
2042 1368 1074 894 736 648 558 359
342 159 130 25
MspA1I CmG'CkG
Cuts at: 1606 2562 3498 5309 5579 5751 6428 7576 7821
Size: 956 936 1811 270 172 677 1148 245
Cuts at: 7821 1606
Size: 2120
Fragments arranged by size:
2120 1811 1148 956 936 677 270 245
172
NaeI GCC'GGC
Cuts at: 3844 3844
Size: 8335
NarI GG'CG_CC
Cuts at: 2442 4015 5229 2442
Size: 1573 1214 5548
Fragments arranged by size:
5548 1573 1214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7783
NdeI CA'TA_TG
Cuts at: 387 4747 4817 387
Size: 4360 70 3905
Fragments arranged by size:
4360 3905 70
NgoMIV G'CCGG_C
Cuts at: 3842 3842
Size: 8335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8335
NotI GC'GGCC_GC
Cuts at: 5782 5782
Size: 8335
NsiI A_TGCA'T
Cuts at: 4968 4968
Size: 8335
NspI r_CATG'y
Cuts at: 1132 1229 2533 4556 5693 7238 1132
Size: 97 1304 2023 1137 1545 2229
Fragments arranged by size:
2229 2023 1545 1304 1137 97
PasI CC'CwG_GG
Cuts at: 1348 3988 1348
Size: 2640 5695
PciI A'CATG_T
Cuts at: 2529 7234 2529
Size: 4705 3630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 3920 1108
Size: 1149 1663 5523
Fragments arranged by size:
5523 1663 1149
PfoI T'CCnGG_A
Cuts at: 2015 2250 4398 4410 4782 2015
Size: 235 2148 12 372 5568
Fragments arranged by size:
5568 2148 372 235 12
PmeI GTTT'AAAC
Cuts at: 5639 5639
Size: 8335
PmlI CAC'GTG
Cuts at: 2781 4324 2781
Size: 1543 6792
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6404 6436 7917 7949 6404
Size: 32 1481 32 6790
Fragments arranged by size:
6790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3450 5383 3450
Size: 1933 6402
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8335
PsiI TTA'TAA
Cuts at: 3476 5905 3476
Size: 2429 5906
PspOMI G'GGCC_C
Cuts at: 3553 3960 5475 3553
Size: 407 1515 6413
Fragments arranged by size:
6413 1515 407
PstI C_TGCA'G
Cuts at: 830 2319 2719 4056 5401 5687 830
Size: 1489 400 1337 1345 286 3478
Fragments arranged by size:
3478 1489 1345 1337 400 286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8335
PvuII CAG'CTG
Cuts at: 2562 3498 5579 2562
Size: 936 2081 5318
Fragments arranged by size:
5318 2081 936
SacI G_AGCT'C
Cuts at: 5114 5114
Size: 8335
SalI G'TCGA_C
Cuts at: 1936 5677 1936
Size: 3741 4594
SbfI CC_TGCA'GG
Cuts at: 5687 5687
Size: 8335
ScaI AGT'ACT
Cuts at: 6583 8295 6583
Size: 1712 6623
SfcI C'TryA_G
Cuts at: 826 1045 2315 2715 4052 4980 5397 5683 6818
Size: 219 1270 400 1337 928 417 286 1135
Cuts at: 6818 7499 7690 826
Size: 681 191 1471
Fragments arranged by size:
1471 1337 1270 1135 928 681 417 400
286 219 191
SfoI GGC'GCC
Cuts at: 2443 4016 5230 2443
Size: 1573 1214 5548
Fragments arranged by size:
5548 1573 1214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8335
SmaI CCC'GGG
Cuts at: 1872 2735 5662 1872
Size: 863 2927 4545
Fragments arranged by size:
4545 2927 863
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3362 4114 6439 7340 7602 820
Size: 197 926 1419 752 2325 901 262 1553
Fragments arranged by size:
2325 1553 1419 926 901 752 262 197
SnaBI TAC'GTA
Cuts at: 493 6126 493
Size: 5633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8335
SphI G_CATG'C
Cuts at: 1132 5693 1132
Size: 4561 3774
SspI AAT'ATT
Cuts at: 5 52 3070 4429 6259 5
Size: 47 3018 1359 1830 2081
Fragments arranged by size:
3018 2081 1830 1359 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8335
StyI C'CwwG_G
Cuts at: 513 1065 2098 2188 3345 3564 3641 5538 5774
Size: 552 1033 90 1157 219 77 1897 236
Cuts at: 5774 513
Size: 3074
Fragments arranged by size:
3074 1897 1157 1033 552 236 219 90
77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3696 4099 4580 4828 6362 6379 6532
Size: 1255 2116 403 481 248 1534 17 153
Cuts at: 6532 6717 7162 325
Size: 185 445 1498
Fragments arranged by size:
2116 1534 1498 1255 481 445 403 248
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 6581 8293
Size: 274 80 33 51 1443 360 4244 1712
Cuts at: 8293 96
Size: 138
Fragments arranged by size:
4244 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3139 3171 4614 4646 8295 8327 3139
Size: 32 1443 32 3649 32 3147
Fragments arranged by size:
3649 3147 1443 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2504 2969 3572 4406 1316
Size: 1188 465 603 834 5245
Fragments arranged by size:
5245 1188 834 603 465
XbaI T'CTAG_A
Cuts at: 5671 5671
Size: 8335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2258 2830 3952 4531 2254
Size: 4 572 1122 579 6058
Fragments arranged by size:
6058 1122 579 572 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8335
XmaI C'CCGG_G
Cuts at: 1870 2733 5660 1870
Size: 863 2927 4545
Fragments arranged by size:
4545 2927 863
XmnI GAAnn'nnTTC
Cuts at: 6464 6464
Size: 8335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6143 276
Size: 53 83 186 936 4609 2468
Fragments arranged by size:
4609 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I EcoRV FalI FseI FspAI
NruI PacI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI