Restriction map for
pFN21AB4447
(Circular) (Six-base) MAPSORT of: pFN21AB4447.seq Check: 2772 from: 1 to: 5986
pFN21AB4447 5986 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2040 2565 2040
Size: 525 5461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 2771 3307 2771
Size: 536 5450
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2241 2468 2766 2814 3043 4049 5433
Size: 479 130 227 298 48 229 1006 1384
Cuts at: 5433 5701 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1006 479 298 268 229 227
130 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2698 4885 5862 1203
Size: 1495 2187 977 1327
Fragments arranged by size:
2187 1495 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 2211 4715 2211
Size: 2504 3482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 5954
AlwNI CAG_nnn'CTG
Cuts at: 3013 3131 3729 5301 3013
Size: 118 598 1572 3698
Fragments arranged by size:
3698 1572 598 118
ApaLI G'TGCA_C
Cuts at: 2053 4043 5199 2053
Size: 1990 1156 2840
Fragments arranged by size:
2840 1990 1156
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2586 3311 1870
Size: 73 80 563 725 4545
Fragments arranged by size:
4545 725 563 80 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2460 2771 2788 3307
Size: 325 217 627 298 375 311 17 519
Cuts at: 3307 4762 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 519 375 325 311 298
217 17
BanII G_rGCy'C
Cuts at: 1391 2547 2586 3020 3305 1391
Size: 1156 39 434 285 4072
Fragments arranged by size:
4072 1156 434 285 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 928
Size: 971 855 4160
Fragments arranged by size:
4160 971 855
BbvCI CC'TCA_GC
Cuts at: 2607 2607
Size: 5986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4177 4211 1903
Size: 34 50 34 2156 34 3678
Fragments arranged by size:
3678 2156 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2911 3878 5088 2911
Size: 967 1210 3809
Fragments arranged by size:
3809 1210 967
BclI T'GATC_A
Cuts at: 1548 2170 1548
Size: 622 5364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 2057 4047 5203 1790
Size: 267 1990 1156 2573
Fragments arranged by size:
2573 1990 1156 267
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3082 3717 4675
Size: 416 53 83 399 24 1665 635 958
Cuts at: 4675 442
Size: 1753
Fragments arranged by size:
1753 1665 958 635 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3117 3117
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2209 2600 4646 1239
Size: 970 391 2046 2579
Fragments arranged by size:
2579 2046 970 391
Bpu10I CC'TnA_GC
Cuts at: 2607 2607
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3214 4111 4976 5274 3056
Size: 158 897 865 298 3768
Fragments arranged by size:
3768 897 865 298 158
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2701 3777 493
Size: 711 1497 1076 2702
Fragments arranged by size:
2702 1497 1076 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2558 2588 2621 2651 1178
Size: 30 165 30 1155 30 33 30 4513
Fragments arranged by size:
4513 1155 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 1186
Size: 949 5037
BseYI C'CCAG_C
Cuts at: 2197 2201 2274 3013 5189 5685 2197
Size: 4 73 739 2176 496 2498
Fragments arranged by size:
2498 2176 739 496 73 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 2909 1163
Size: 573 882 291 4240
Fragments arranged by size:
4240 882 573 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2057 2523 2547 4047 4132 5203 2057
Size: 466 24 1500 85 1071 2840
Fragments arranged by size:
2840 1500 1071 466 85 24
BsmI GAATG_Cn'
Cuts at: 3236 3497 3590 3236
Size: 261 93 5632
Fragments arranged by size:
5632 261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2523 2547 2586 3020 3305 4047
Size: 399 267 466 24 39 434 285 742
Cuts at: 4047 4132 5203 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 434 399 285 267
85 39 24
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2040 2565 2640 3139 3341 844
Size: 1196 525 75 499 202 3489
Fragments arranged by size:
3489 1196 525 499 202 75
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 4481 4655 65
Size: 1183 925 2308 174 1396
Fragments arranged by size:
2308 1396 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2571 4630 4817 1700
Size: 110 761 2059 187 2869
Fragments arranged by size:
2869 2059 761 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 2471 4046 5058 2471
Size: 1575 1012 3399
Fragments arranged by size:
3399 1575 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 794
Size: 1220 4766
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2659 2940 3316 3683 4068 4085 5537 5623
Size: 687 281 376 367 385 17 1452 86
Cuts at: 5623 5635 5981 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 687 385 376 367 346 281
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2038 2038
Size: 5986
BtgI C'CryG_G
Cuts at: 513 1065 2280 2869 513
Size: 552 1215 589 3630
Fragments arranged by size:
3630 1215 589 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2376 2443 2832 3502 4296 4316 2087
Size: 289 67 389 670 794 20 3757
Fragments arranged by size:
3757 794 670 389 289 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DraIII CAC_nnn'GTG
Cuts at: 2704 2704
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2337 2613 2961
Size: 54 1425 50 275 18 507 276 348
Cuts at: 2961 3210 3433 4322 5951 8
Size: 249 223 889 1629 43
Fragments arranged by size:
1629 1425 889 507 348 276 275 249
223 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2552 3916 1353
Size: 1199 1364 3423
Fragments arranged by size:
3423 1364 1199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2121 4573 4947 5093 387
Size: 1064 138 532 2452 374 146 1280
Fragments arranged by size:
2452 1280 1064 532 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 2545
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2209 2241 2468 2600 2766 2814
Size: 393 479 98 32 227 132 166 48
Cuts at: 2814 3043 4049 4646 5433 5701 1239
Size: 229 1006 597 787 268 1524
Fragments arranged by size:
1524 1006 787 597 479 393 268 229
227 166 132 98 48 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2026 2726 2026
Size: 700 5286
EcoO109I rG'GnC_Cy
Cuts at: 2205 2252 2329 3128 3225 2205
Size: 47 77 799 97 4966
Fragments arranged by size:
4966 799 97 77 47
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2530 2413
Size: 117 5869
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2572 2622 5133 1678
Size: 894 50 2511 2531
Fragments arranged by size:
2531 2511 894 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2197 2229 2741 2773 4627
Size: 32 135 32 625 32 512 32 1854
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1854 625 512 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 3330 3576 669
Size: 988 281 595 797 246 3079
Fragments arranged by size:
3079 988 797 595 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 2775 3311 2775
Size: 536 5450
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2339 2615 3212 10
Size: 54 1425 343 507 276 597 2784
Fragments arranged by size:
2784 1425 597 507 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3944 4303 4462 518
Size: 648 2778 359 159 2042
Fragments arranged by size:
2778 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2201 2563 3010 3402 4079 5227 5472 1606
Size: 595 362 447 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 447 392 362 245
NcoI C'CATG_G
Cuts at: 513 1065 2869 513
Size: 552 1804 3630
Fragments arranged by size:
3630 1804 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 3072 1108
Size: 1317 647 4022
Fragments arranged by size:
4022 1317 647
PfoI T'CCnGG_A
Cuts at: 2577 2577
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PmlI CAC'GTG
Cuts at: 2701 2701
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2590 4055 4087 5568 5600 2558
Size: 32 1465 32 1481 32 2944
Fragments arranged by size:
2944 1481 1465 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2329 3128 2205
Size: 124 799 5063
Fragments arranged by size:
5063 799 124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2562 3338 830
Size: 1732 776 3478
Fragments arranged by size:
3478 1732 776
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2201 2201
Size: 5986
SacI G_AGCT'C
Cuts at: 2547 2547
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 5986
SapI GCTCTTCn'nnn_
Cuts at: 2552 2552
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 2783 4234 5946 2783
Size: 1451 1712 2823
Fragments arranged by size:
2823 1712 1451
SfcI C'TryA_G
Cuts at: 826 1045 2179 2381 2558 2887 2936 3334 4469
Size: 219 1134 202 177 329 49 398 1135
Cuts at: 4469 5150 5341 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1134 681 398 329 219 202
191 177 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2588 3313 1872
Size: 716 725 4545
Fragments arranged by size:
4545 725 716
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3035 3229 4090 4991 5253 820
Size: 197 926 1092 194 861 901 262 1553
Fragments arranged by size:
1553 1092 926 901 861 262 197 194
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2869 3425 513
Size: 552 1804 556 3074
Fragments arranged by size:
3074 1804 556 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3179 4013 4030 4183 4368 4813 325
Size: 1255 1599 834 17 153 185 445 1498
Fragments arranged by size:
1599 1498 1255 834 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 4232 5944
Size: 274 80 33 51 1443 804 1451 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1712 1451 1443 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2688 2720 2822 2854 5946 5978 2688
Size: 32 102 32 3092 32 2696
Fragments arranged by size:
3092 2696 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2528 1316
Size: 1212 4774
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 2586 3311 1870
Size: 716 725 4545
Fragments arranged by size:
4545 725 716
XmnI GAAnn'nnTTC
Cuts at: 2799 4115 2799
Size: 1316 4670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI