Restriction map for
pFN21AB4421
(Circular) (Six-base) MAPSORT of: pFN21AB4421.seq Check: 4933 from: 1 to: 6280
pFN21AB4421 6280 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3573 3573
Size: 6280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3623 1937
Size: 1243 443 4594
Fragments arranged by size:
4594 1243 443
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 3150 4343 5727 5995 1632
Size: 1049 469 1193 1384 268 1917
Fragments arranged by size:
1917 1384 1193 1049 469 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 3164 5179 6156 1203
Size: 1961 2015 977 1327
Fragments arranged by size:
2015 1961 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 2817 1166
Size: 1651 4629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3282 3314 3282
Size: 32 6248
AlwNI CAG_nnn'CTG
Cuts at: 4023 5595 4023
Size: 1572 4708
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 2216 3353 3589 3815 3967 6076 1493
Size: 723 1137 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1137 723 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2849 4834 160
Size: 872 1817 1985 1606
Fragments arranged by size:
1985 1817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 3605 1870
Size: 73 1662 4545
Fragments arranged by size:
4545 1662 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 3601 5056 618
Size: 325 217 627 1697 117 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2123 3105 3599 1391
Size: 732 982 494 4072
Fragments arranged by size:
4072 982 732 494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2586 928
Size: 971 687 4622
Fragments arranged by size:
4622 971 687
BbvCI CC'TCA_GC
Cuts at: 2067 2067
Size: 6280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4471 4505 1903
Size: 34 2534 34 3678
Fragments arranged by size:
3678 2534 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2968 6275 2968
Size: 3307 2973
BlpI GC'TnA_GC
Cuts at: 3697 3697
Size: 6280
Bme1580I G_kGCm'C
Cuts at: 1790 4341 5497 1790
Size: 2551 1156 2573
Fragments arranged by size:
2573 2551 1156
BmgBI CAC'GTC
Cuts at: 1318 3167 1318
Size: 1849 4431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2361 2895 3082
Size: 416 53 83 399 24 944 534 187
Cuts at: 3082 3341 4011 4969 442
Size: 259 670 958 1753
Fragments arranged by size:
1753 958 944 670 534 416 399 259
187 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2465 2889 3054 4940 1239
Size: 917 309 424 165 1886 2579
Fragments arranged by size:
2579 1886 917 424 309 165
Bpu10I CC'TnA_GC
Cuts at: 2067 2067
Size: 6280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 4405 5270 5568 2540
Size: 1865 865 298 3252
Fragments arranged by size:
3252 1865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2649 4943 882
Size: 617 1150 2294 2219
Fragments arranged by size:
2294 2219 1150 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4087 4469 5104 275
Size: 53 83 186 936 2554 382 635 1451
Fragments arranged by size:
2554 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4713 5111 5385 5532 5943 4713
Size: 398 274 147 411 5050
Fragments arranged by size:
5050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6055
Fragments arranged by size:
6055 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2550 1186
Size: 967 397 4916
Fragments arranged by size:
4916 967 397
BseYI C'CCAG_C
Cuts at: 2038 5483 5979 2038
Size: 3445 496 2339
Fragments arranged by size:
3445 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5707
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2875 3564 4341 4426 5497 2875
Size: 689 777 85 1071 3658
Fragments arranged by size:
3658 1071 777 689 85
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2875 3105 3564 3599 4341 4426
Size: 399 333 752 230 459 35 742 85
Cuts at: 4426 5497 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 752 742 459 399 333 230
85 35
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 2737 4169 5899 2737
Size: 1432 1730 3118
Fragments arranged by size:
3118 1730 1432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3573 3635 844
Size: 2729 62 3489
Fragments arranged by size:
3489 2729 62
BsrBI CCG'CTC
Cuts at: 1394 1823 4167 1394
Size: 429 2344 3507
Fragments arranged by size:
3507 2344 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2394 4775 4949 65
Size: 1183 1146 2381 174 1396
Fragments arranged by size:
2381 1396 1183 1146 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4924 5111 1700
Size: 110 3114 187 2869
Fragments arranged by size:
3114 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6280
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 2620 2620
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 2968 3610 3977 4362 4379 5831 5917 5929
Size: 996 642 367 385 17 1452 86 12
Cuts at: 5929 6275 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 996 642 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2170 2613 513
Size: 552 1105 443 4180
Fragments arranged by size:
4180 1105 552 443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 3796 4590 4610 2583
Size: 1213 794 20 4253
Fragments arranged by size:
4253 1213 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3112 3584 3112
Size: 472 5808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 3390 3554
Size: 54 1425 50 275 18 979 581 164
Cuts at: 3554 3727 4616 6245 8
Size: 173 889 1629 43
Fragments arranged by size:
1629 1425 979 889 581 275 173 164
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3126 3373 4210 1353
Size: 1773 247 837 3423
Fragments arranged by size:
3423 1773 837 247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2465 2681 2889 3054 3150 4343
Size: 393 524 309 216 208 165 96 1193
Cuts at: 4343 4940 5727 5995 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1193 787 597 524 393 309 268
216 208 165 96
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 3301 3402 2479
Size: 822 101 5357
Fragments arranged by size:
5357 822 101
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 5427 1678
Size: 3749 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 4921 4953 4995
Size: 32 135 32 533 32 2784 32 42
Cuts at: 4995 5027 1373
Size: 32 2626
Fragments arranged by size:
2784 2626 533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3181 3624 3870 669
Size: 988 281 1243 443 246 3079
Fragments arranged by size:
3079 1243 988 443 281 246
HindIII A'AGCT_T
Cuts at: 748 3531 748
Size: 2783 3497
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 2907 3879 2907
Size: 972 5308
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 5394 5578 1305
Size: 856 3233 184 2007
Fragments arranged by size:
3233 2007 856 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2811 10
Size: 54 1425 343 979 3479
Fragments arranged by size:
3479 1425 979 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 2817 4238 4597 4756 518
Size: 648 1206 445 1421 359 159 2042
Fragments arranged by size:
2042 1421 1206 648 445 359 159
MspA1I CmG'CkG
Cuts at: 1606 3696 4373 5521 5766 1606
Size: 2090 677 1148 245 2120
Fragments arranged by size:
2120 2090 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2170 2613 513
Size: 552 1105 443 4180
Fragments arranged by size:
4180 1105 552 443
NdeI CA'TA_TG
Cuts at: 387 2188 3241 387
Size: 1801 1053 3426
Fragments arranged by size:
3426 1801 1053
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2863 3638 5183 1132
Size: 97 1634 775 1545 2229
Fragments arranged by size:
2229 1634 1545 775 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6280
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2885 1108
Size: 1777 4503
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3176 1613
Size: 1563 4717
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PstI C_TGCA'G
Cuts at: 830 3632 830
Size: 2802 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3179 3622 1936
Size: 1243 443 4594
Fragments arranged by size:
4594 1243 443
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SfcI C'TryA_G
Cuts at: 826 1045 2650 3628 4763 5444 5635 826
Size: 219 1605 978 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 978 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 4384 5285 5547 820
Size: 197 926 576 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 576 262 197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 2234 152
Size: 2082 4198
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 2361 2440 2571 4204 5
Size: 47 2309 79 131 1633 2081
Fragments arranged by size:
2309 2081 1633 131 79 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2170 2613 2689 3719 513
Size: 552 1105 443 76 1030 3074
Fragments arranged by size:
3074 1105 1030 552 443 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4307 4324 4477 4662 5107 325
Size: 1255 2727 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3256 4526 6238
Size: 274 80 33 51 1443 1279 1270 1712
Cuts at: 6238 96
Size: 138
Fragments arranged by size:
1712 1443 1279 1270 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3388 3420 6240 6272 3388
Size: 32 2820 32 3396
Fragments arranged by size:
3396 2820 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2177
Size: 6280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 2733 3081 4409 2733
Size: 348 1328 4604
Fragments arranged by size:
4604 1328 348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI