Restriction map for
pFN21AB4417
(Circular) (Six-base) MAPSORT of: pFN21AB4417.seq Check: 6019 from: 1 to: 6175
pFN21AB4417 6175 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2948 4238 5622 5890 1632
Size: 422 894 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 894 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 2899 1166
Size: 1733 4442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2571 3356 1184
Size: 1387 785 4003
Fragments arranged by size:
4003 1387 785
AlwNI CAG_nnn'CTG
Cuts at: 2032 2464 2542 2587 3027 3102 3918 5490 2032
Size: 432 78 45 440 75 816 1572 2717
Fragments arranged by size:
2717 1572 816 440 432 78 75 45
ApaI G_GGCC'C
Cuts at: 2214 2517 2214
Size: 303 5872
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 3484 3710 3862 5971 1493
Size: 1991 226 152 2109 1697
Fragments arranged by size:
2109 1991 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2727 3500 1870
Size: 73 784 773 4545
Fragments arranged by size:
4545 784 773 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2006 2039 2006
Size: 33 6142
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2467 2555 3332 3496
Size: 325 217 627 251 429 88 777 164
Cuts at: 3496 4951 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 777 627 429 325 251 217
164 88
BanII G_rGCy'C
Cuts at: 1391 2055 2161 2214 2421 2517 3494 1391
Size: 664 106 53 207 96 977 4072
Fragments arranged by size:
4072 977 664 207 106 96 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5204
BbvCI CC'TCA_GC
Cuts at: 3439 3439
Size: 6175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2512 2775 2879
Size: 107 122 71 631 605 840 263 104
Cuts at: 2879 4786 136
Size: 1907 1525
Fragments arranged by size:
1907 1525 840 631 605 263 122 107
104 71
BglII A'GATC_T
Cuts at: 2742 2844 6170 2742
Size: 102 3326 2747
Fragments arranged by size:
3326 2747 102
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2517 2560 3335 4236 5392 1790
Size: 424 303 43 775 901 1156 2573
Fragments arranged by size:
2573 1156 901 775 424 303 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3415 3906 4864
Size: 416 53 83 399 24 1998 491 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
1998 1753 958 491 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2904 3023 3404 4835 1239
Size: 1665 119 381 1431 2579
Fragments arranged by size:
2579 1665 1431 381 119
Bpu10I CC'TnA_GC
Cuts at: 3439 3439
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2724 4838 882
Size: 617 1225 2114 2219
Fragments arranged by size:
2219 2114 1225 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 3075 3982 4364
Size: 53 83 186 936 1065 477 907 382
Cuts at: 4364 4999 275
Size: 635 1451
Fragments arranged by size:
1451 1065 936 907 635 477 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 3395 3425 1178
Size: 30 165 30 954 30 1008 30 3928
Fragments arranged by size:
3928 1008 954 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2453 1186
Size: 877 390 4908
Fragments arranged by size:
4908 877 390
BseYI C'CCAG_C
Cuts at: 2115 2224 2332 2427 2507 2517 2692 3102 5378
Size: 109 108 95 80 10 175 410 2276
Cuts at: 5378 5874 2115
Size: 496 2416
Fragments arranged by size:
2416 2276 496 410 175 109 108 95
80 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5602
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4236 4321 5392 4236
Size: 85 1071 5019
Fragments arranged by size:
5019 1071 85
BsmI GAATG_Cn'
Cuts at: 2268 3686 3779 2268
Size: 1418 93 4664
Fragments arranged by size:
4664 1418 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3317 3317
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2161 2214 2421 2517 2560 3335
Size: 399 265 106 53 207 96 43 775
Cuts at: 3335 3494 4236 4321 5392 1391
Size: 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 399 265 207 159
106 96 85 53 43
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 2982 4064 5794 2982
Size: 1082 1730 3363
Fragments arranged by size:
3363 1730 1082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3000 3530 844
Size: 1697 459 530 3489
Fragments arranged by size:
3489 1697 530 459
BsrBI CCG'CTC
Cuts at: 1394 1823 2553 4062 1394
Size: 429 730 1509 3507
Fragments arranged by size:
3507 1509 730 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4670 4844 65
Size: 1183 3422 174 1396
Fragments arranged by size:
3422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 4819 5006 1700
Size: 110 535 2474 187 2869
Fragments arranged by size:
2869 2474 535 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2542 3462 794
Size: 1748 920 3507
Fragments arranged by size:
3507 1748 920
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstXI CCAn_nnnn'nTGG
Cuts at: 2796 2796
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2742 2844 3505 3872 4257 4274 5726 5812
Size: 770 102 661 367 385 17 1452 86
Cuts at: 5812 5824 6170 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 770 661 385 367 346 102
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2274 3398 2274
Size: 1124 5051
BtgI C'CryG_G
Cuts at: 513 1065 2301 2593 3207 513
Size: 552 1236 292 614 3481
Fragments arranged by size:
3481 1236 614 552 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2353 2476 487
Size: 683 56 1127 123 4186
Fragments arranged by size:
4186 1127 683 123 56
BtsI GCAGTG_nn'
Cuts at: 2259 2656 3691 4485 4505 2259
Size: 397 1035 794 20 3929
Fragments arranged by size:
3929 1035 794 397 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2911 3622 4511
Size: 54 1425 50 275 18 1081 711 889
Cuts at: 4511 6140 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1081 889 711 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4105 1353
Size: 2752 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 4762 5136 5282 387
Size: 1064 138 954 2219 374 146 1280
Fragments arranged by size:
2219 1280 1064 954 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2904 2948 3023 3404 4238 4835
Size: 393 422 850 44 75 381 834 597
Cuts at: 4835 5622 5890 1239
Size: 787 268 1524
Fragments arranged by size:
1524 850 834 787 597 422 393 381
268 75 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2768 2768
Size: 6175
EcoO109I rG'GnC_Cy
Cuts at: 2119 2278 2443 2514 2119
Size: 159 165 71 5780
Fragments arranged by size:
5780 165 159 71
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
FspI TGC'GCA
Cuts at: 2352 4681 2352
Size: 2329 3846
FspAI rTGC'GCAy
Cuts at: 2352 2352
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 2224 5322 1678
Size: 546 3098 2531
Fragments arranged by size:
3098 2531 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 3395 3427 4816
Size: 32 135 32 785 32 1006 32 1389
Cuts at: 4816 4848 4890 4922 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1389 1006 785 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3247 3519 3765 669
Size: 988 281 1309 272 246 3079
Fragments arranged by size:
3079 1309 988 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 5289 5473 1305
Size: 2016 1968 184 2007
Fragments arranged by size:
2016 2007 1968 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2899 3185 4133 4492 4651 518
Size: 648 1329 404 286 948 359 159 2042
Fragments arranged by size:
2042 1329 948 648 404 359 286 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2173 2455 2511 2718 3099 3209 3382
Size: 509 58 282 56 207 381 110 173
Cuts at: 3382 3443 3591 4268 5416 5661 1606
Size: 61 148 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 509 381 282 245 207
173 148 110 61 58 56
NcoI C'CATG_G
Cuts at: 513 1065 2593 513
Size: 552 1528 4095
Fragments arranged by size:
4095 1528 552
NdeI CA'TA_TG
Cuts at: 387 3235 387
Size: 2848 3327
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 2581 3533 5078 1132
Size: 97 1352 952 1545 2229
Fragments arranged by size:
2229 1545 1352 952 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2616 1108
Size: 1508 4667
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 2883 4244 4276 5757 5789 2851
Size: 32 1361 32 1481 32 3237
Fragments arranged by size:
3237 1481 1361 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2443 2119
Size: 324 5851
PshAI GACnn'nnGTC
Cuts at: 1613 2681 1613
Size: 1068 5107
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PspOMI G'GGCC_C
Cuts at: 2210 2513 2210
Size: 303 5872
PstI C_TGCA'G
Cuts at: 830 2047 2460 2538 2550 2717 3527 830
Size: 1217 413 78 12 167 810 3478
Fragments arranged by size:
3478 1217 810 413 167 78 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2173 2455 2718 3099 3382 3443 2173
Size: 282 263 381 283 61 4905
Fragments arranged by size:
4905 381 283 282 263 61
SacII CC_GC'GG
Cuts at: 3210 3210
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 2538 3527 2538
Size: 989 5186
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2043 2456 2534 2546 2713 3523 4658
Size: 219 998 413 78 12 167 810 1135
Cuts at: 4658 5339 5530 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 998 810 681 413 219 191
167 78 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 2345 1700
Size: 645 5530
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2727 4279 5180 5442 820
Size: 197 926 784 1552 901 262 1553
Fragments arranged by size:
1553 1552 926 901 784 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 2327 2753 1517
Size: 810 426 4939
Fragments arranged by size:
4939 810 426
StyI C'CwwG_G
Cuts at: 513 1065 2593 3614 513
Size: 552 1528 1021 3074
Fragments arranged by size:
3074 1528 1021 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 2608 4202 4219 4372 4557 5002
Size: 1255 840 188 1594 17 153 185 445
Cuts at: 5002 325
Size: 1498
Fragments arranged by size:
1594 1498 1255 840 445 188 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3258 4421
Size: 274 80 33 51 1443 729 552 1163
Cuts at: 4421 6133 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1163 729 552 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 2727 1943
Size: 784 5391
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 4304 2268
Size: 2036 4139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI