(Circular) (Six-base) MAPSORT of: pFN21AB4406.seq Check: 7781 from: 1 to: 11347 pFN21AB4406 11347 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5115 7543 7824 5115 Size: 2428 281 8638 Fragments arranged by size: 8638 2428 281 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4437 8631 9157 278 Size: 53 83 186 936 2901 4194 526 2468 Fragments arranged by size: 4194 2901 2468 936 526 186 83 53 AccI GT'mk_AC Cuts at: 1937 3273 6774 8690 1937 Size: 1336 3501 1916 4594 Fragments arranged by size: 4594 3501 1916 1336 Acc65I G'GTAC_C Cuts at: 6324 7741 8668 6324 Size: 1417 927 9003 Fragments arranged by size: 9003 1417 927 AclI AA'CG_TT Cuts at: 1563 9474 9847 1563 Size: 7911 373 3063 Fragments arranged by size: 7911 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3164 3347 4718 6671 8285 9410 10794 11062 Size: 1532 183 1371 1953 1614 1125 1384 268 Cuts at: 11062 1632 Size: 1917 Fragments arranged by size: 1953 1917 1614 1532 1384 1371 1125 268 183 AfeI AGC'GCT Cuts at: 2251 4862 6473 6602 2251 Size: 2611 1611 129 6996 Fragments arranged by size: 6996 2611 1611 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11150 AflIII A'CryG_T Cuts at: 1203 2412 2743 2922 3561 6257 6452 8190 8283 Size: 1209 331 179 639 2696 195 1738 93 Cuts at: 8283 10246 11223 1203 Size: 1963 977 1327 Fragments arranged by size: 2696 1963 1738 1327 1209 977 639 331 195 179 93 AgeI A'CCGG_T Cuts at: 10178 10178 Size: 11347 AhdI GACnn_n'nnGTC Cuts at: 10076 10076 Size: 11347 AleI CACnn'nnGTG Cuts at: 1166 3209 5598 6311 1166 Size: 2043 2389 713 6202 Fragments arranged by size: 6202 2389 2043 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4911 1184 Size: 3727 7620 AlwNI CAG_nnn'CTG Cuts at: 2107 3773 5559 5872 8084 9090 10662 2107 Size: 1666 1786 313 2212 1006 1572 2792 Fragments arranged by size: 2792 2212 1786 1666 1572 1006 313 ApaI G_GGCC'C Cuts at: 6252 6252 Size: 11347 ApaLI G'TGCA_C Cuts at: 2324 9404 10560 2324 Size: 7080 1156 3111 Fragments arranged by size: 7080 3111 1156 ApoI r'AATT_y Cuts at: 1493 2445 4115 8253 8656 8882 9034 11143 1493 Size: 952 1670 4138 403 226 152 2109 1697 Fragments arranged by size: 4138 2109 1697 1670 952 403 226 152 AseI AT'TA_AT Cuts at: 160 1032 3042 3291 9901 160 Size: 872 2010 249 6610 1606 Fragments arranged by size: 6610 2010 1606 872 249 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11347 AvaI C'yCGr_G Cuts at: 1870 1943 2264 2367 2862 4038 4330 8672 1870 Size: 73 321 103 495 1176 292 4342 4545 Fragments arranged by size: 4545 4342 1176 495 321 292 103 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8177 8210 8177 Size: 33 11314 BamHI G'GATC_C Cuts at: 5695 8677 9044 5695 Size: 2982 367 7998 Fragments arranged by size: 7998 2982 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6324 7741 8425 8668 10123 Size: 325 217 627 4537 1417 684 243 1455 Cuts at: 10123 618 Size: 1842 Fragments arranged by size: 4537 1842 1455 1417 684 627 325 243 217 BanII G_rGCy'C Cuts at: 1391 2083 2271 4645 5016 5823 6252 7027 8666 Size: 692 188 2374 371 807 429 775 1639 Cuts at: 8666 1391 Size: 4072 Fragments arranged by size: 4072 2374 1639 807 775 692 429 371 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2827 2961 3784 5231 6455 6812 928 Size: 971 928 134 823 1447 1224 357 5463 Fragments arranged by size: 5463 1447 1224 971 928 823 357 134 BbvCI CC'TCA_GC Cuts at: 4529 4529 Size: 11347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2335 2369 5912 5946 9538 9572 1903 Size: 34 398 34 3543 34 3592 34 3678 Fragments arranged by size: 3678 3592 3543 398 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5408 5659 9239 10449 5408 Size: 251 3580 1210 6306 Fragments arranged by size: 6306 3580 1210 251 BclI T'GATC_A Cuts at: 1548 4479 8113 1548 Size: 2931 3634 4782 Fragments arranged by size: 4782 3634 2931 BfrBI ATG'CAT Cuts at: 8378 8378 Size: 11347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 5190 5742 Size: 107 122 71 631 605 535 2983 552 Cuts at: 5742 6501 8313 9958 136 Size: 759 1812 1645 1525 Fragments arranged by size: 2983 1812 1645 1525 759 631 605 552 535 122 107 71 BglII A'GATC_T Cuts at: 7915 8322 8399 11342 7915 Size: 407 77 2943 7920 Fragments arranged by size: 7920 2943 407 77 BlpI GC'TnA_GC Cuts at: 3438 4946 8450 8764 3438 Size: 1508 3504 314 6021 Fragments arranged by size: 6021 3504 1508 314 Bme1580I G_kGCm'C Cuts at: 1790 2328 4659 5073 5634 6252 8430 9408 10564 Size: 538 2331 414 561 618 2178 978 1156 Cuts at: 10564 1790 Size: 2573 Fragments arranged by size: 2573 2331 2178 1156 978 618 561 538 414 BmgBI CAC'GTC Cuts at: 1318 6453 7632 1318 Size: 5135 1179 5033 Fragments arranged by size: 5135 5033 1179 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 2494 2551 Size: 416 53 83 399 24 921 156 57 Cuts at: 2551 3013 4555 4696 4936 5362 7030 8036 9078 Size: 462 1542 141 240 426 1668 1006 1042 Cuts at: 9078 10036 442 Size: 958 1753 Fragments arranged by size: 1753 1668 1542 1042 1006 958 921 462 426 416 399 240 156 141 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 3552 1056 Size: 2496 8851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 3354 3449 3489 3603 3827 4517 4844 Size: 1116 999 95 40 114 224 690 327 Cuts at: 4844 6833 6959 7225 8524 10007 1239 Size: 1989 126 266 1299 1483 2579 Fragments arranged by size: 2579 1989 1483 1299 1116 999 690 327 266 224 126 114 95 40 Bpu10I CC'TnA_GC Cuts at: 4529 4529 Size: 11347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 5186 5444 7532 9472 10337 10635 2906 Size: 2280 258 2088 1940 865 298 3618 Fragments arranged by size: 3618 2280 2088 1940 865 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2992 3627 3704 4686 6809 10010 882 Size: 617 1493 635 77 982 2123 3201 2219 Fragments arranged by size: 3201 2219 2123 1493 982 635 617 77 BsaAI yAC'GTr Cuts at: 493 1204 2413 6258 6870 9138 493 Size: 711 1209 3845 612 2268 2702 Fragments arranged by size: 3845 2702 2268 1209 711 612 BsaBI GATnn'nnATC Cuts at: 5694 11341 5694 Size: 5647 5700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4434 8628 9154 9536 Size: 53 83 186 936 2901 4194 526 382 Cuts at: 9536 10171 275 Size: 635 1451 Fragments arranged by size: 4194 2901 1451 936 635 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2235 3471 4111 4188 8490 9780 10178 10452 10599 Size: 1236 640 77 4302 1290 398 274 147 Cuts at: 10599 11010 2235 Size: 411 2572 Fragments arranged by size: 4302 2572 1290 1236 640 411 398 274 147 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4209 4236 4239 4266 6548 Size: 30 165 30 2806 27 3 27 2282 Cuts at: 6548 6578 8522 8552 1178 Size: 30 1944 30 3973 Fragments arranged by size: 3973 2806 2282 1944 165 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 3323 3446 3752 4233 5512 7017 7396 Size: 1435 702 123 306 481 1279 1505 379 Cuts at: 7396 8554 1186 Size: 1158 3979 Fragments arranged by size: 3979 1505 1435 1279 1158 702 481 379 306 123 BseYI C'CCAG_C Cuts at: 3937 5643 8359 8545 10550 11046 3937 Size: 1706 2716 186 2005 496 4238 Fragments arranged by size: 4238 2716 2005 1706 496 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 2177 3862 4018 6357 7521 8391 Size: 573 357 84 1685 156 2339 1164 870 Cuts at: 8391 1163 Size: 4119 Fragments arranged by size: 4119 2339 1685 1164 870 573 357 156 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8797 9558 10586 1540 Size: 275 186 6796 761 1028 2301 Fragments arranged by size: 6796 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2083 2328 3174 3788 4401 6594 9408 9493 10564 Size: 245 846 614 613 2193 2814 85 1071 Cuts at: 10564 2083 Size: 2866 Fragments arranged by size: 2866 2814 2193 1071 846 614 613 245 85 BsmI GAATG_Cn' Cuts at: 3483 6440 8858 8951 3483 Size: 2957 2418 93 5879 Fragments arranged by size: 5879 2957 2418 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2271 2328 3174 3788 4401 4645 Size: 399 293 188 57 846 614 613 244 Cuts at: 4645 4659 5016 5073 5634 5823 6252 6594 7027 Size: 14 357 57 561 189 429 342 433 Cuts at: 7027 8430 8666 9408 9493 10564 1391 Size: 1403 236 742 85 1071 2174 Fragments arranged by size: 2174 1403 1071 846 742 614 613 561 433 429 399 357 342 293 244 236 189 188 85 57 57 14 BspEI T'CCGG_A Cuts at: 2235 3471 4111 11010 2235 Size: 1236 640 6899 2572 Fragments arranged by size: 6899 2572 1236 640 BspHI T'CATG_A Cuts at: 2730 9236 10966 2730 Size: 6506 1730 3111 Fragments arranged by size: 6506 3111 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5115 6302 7543 7824 8702 844 Size: 4271 1187 1241 281 878 3489 Fragments arranged by size: 4271 3489 1241 1187 878 281 BsrBI CCG'CTC Cuts at: 1394 1823 2233 3919 4837 5520 9234 1394 Size: 429 410 1686 918 683 3714 3507 Fragments arranged by size: 3714 3507 1686 918 683 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 3276 3657 4899 5065 5728 9842 10016 Size: 1183 2028 381 1242 166 663 4114 174 Cuts at: 10016 65 Size: 1396 Fragments arranged by size: 4114 2028 1396 1242 1183 663 381 174 166 BsrFI r'CCGG_y Cuts at: 1700 1810 3194 4009 7592 9991 10178 1700 Size: 110 1384 815 3583 2399 187 2869 Fragments arranged by size: 3583 2869 2399 1384 815 187 110 BsrGI T'GTAC_A Cuts at: 96 3564 6669 8478 96 Size: 3468 3105 1809 2965 Fragments arranged by size: 3468 3105 2965 1809 BssHII G'CGCG_C Cuts at: 1925 2121 2189 1925 Size: 196 68 11083 Fragments arranged by size: 11083 196 68 BssSI C'ACGA_G Cuts at: 2150 4652 9407 10419 2150 Size: 2502 4755 1012 3078 Fragments arranged by size: 4755 3078 2502 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2107 8087 794 Size: 1313 5980 4054 Fragments arranged by size: 5980 4054 1313 BstBI TT'CG_AA Cuts at: 6432 10151 6432 Size: 3719 7628 BstEII G'GTnAC_C Cuts at: 1184 3903 1184 Size: 2719 8628 BstXI CCAn_nnnn'nTGG Cuts at: 4645 5387 7043 8327 4645 Size: 742 1656 1284 7665 Fragments arranged by size: 7665 1656 1284 742 BstYI r'GATC_y Cuts at: 1972 4108 5695 5956 7915 8322 8399 8677 9044 Size: 2136 1587 261 1959 407 77 278 367 Cuts at: 9044 9429 9446 10898 10984 10996 11342 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2136 1977 1959 1587 1452 407 385 367 346 278 261 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 5187 6935 5187 Size: 1748 9599 BtgI C'CryG_G Cuts at: 513 1065 2004 3637 3855 5883 7042 7308 7845 Size: 552 939 1633 218 2028 1159 266 537 Cuts at: 7845 8634 513 Size: 789 3226 Fragments arranged by size: 3226 2028 1633 1159 939 789 552 537 266 218 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3040 3502 5340 6427 6651 487 Size: 683 56 1814 462 1838 1087 224 5183 Fragments arranged by size: 5183 1838 1814 1087 683 462 224 56 BtsI GCAGTG_nn' Cuts at: 2063 2407 2962 3205 3583 4380 5155 5632 5812 Size: 344 555 243 378 797 775 477 180 Cuts at: 5812 5873 6142 6292 6381 6658 7581 8863 9657 Size: 61 269 150 89 277 923 1282 794 Cuts at: 9657 9677 2063 Size: 20 3733 Fragments arranged by size: 3733 1282 923 797 794 775 555 477 378 344 277 269 243 180 150 89 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4954 4989 5293 5328 6388 6423 6473 Size: 35 4390 35 304 35 1060 35 50 Cuts at: 6473 6508 529 Size: 35 5368 Fragments arranged by size: 5368 4390 1060 304 50 35 35 35 35 35 DraI TTT'AAA Cuts at: 8148 8251 8651 8148 Size: 103 400 10844 Fragments arranged by size: 10844 400 103 DraIII CAC_nnn'GTG Cuts at: 3253 4750 3253 Size: 1497 9850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2292 10354 809 Size: 507 976 8062 1802 Fragments arranged by size: 8062 1802 976 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 2995 3654 Size: 54 1425 50 275 18 886 279 659 Cuts at: 3654 4852 6139 6481 6502 8794 9683 11312 8 Size: 1198 1287 342 21 2292 889 1629 43 Fragments arranged by size: 2292 1629 1425 1287 1198 889 886 659 342 279 275 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 8794 1537 Size: 275 6982 4090 Fragments arranged by size: 6982 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2598 3776 4266 5220 7655 9277 1353 Size: 1245 1178 490 954 2435 1622 3423 Fragments arranged by size: 3423 2435 1622 1245 1178 954 490 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3657 3979 9934 10308 10454 387 Size: 1064 138 2068 322 5955 374 146 1280 Fragments arranged by size: 5955 2068 1280 1064 374 322 146 138 EcoICRI GAG'CTC Cuts at: 2081 2081 Size: 11347 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 3164 3347 3354 3449 3489 3603 Size: 393 723 809 183 7 95 40 114 Cuts at: 3603 3827 4517 4718 4844 6671 6833 6959 7225 Size: 224 690 201 126 1827 162 126 266 Cuts at: 7225 8285 8524 9410 10007 10794 11062 1239 Size: 1060 239 886 597 787 268 1524 Fragments arranged by size: 1827 1524 1060 886 809 787 723 690 597 393 268 266 239 224 201 183 162 126 126 114 95 40 7 EcoNI CCTnn'n_nnAGG Cuts at: 3211 3491 5099 6730 7105 3211 Size: 280 1608 1631 375 7453 Fragments arranged by size: 7453 1631 1608 375 280 EcoO109I rG'GnC_Cy Cuts at: 2676 4977 5167 6249 6291 6939 2676 Size: 2301 190 1082 42 648 7084 Fragments arranged by size: 7084 2301 1082 648 190 42 EcoRI G'AATT_C Cuts at: 8656 9034 8656 Size: 378 10969 EcoRV GAT'ATC Cuts at: 7261 7321 7261 Size: 60 11287 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 2900 7495 8160 2479 Size: 421 4595 665 5666 Fragments arranged by size: 5666 4595 665 421 FspI TGC'GCA Cuts at: 9853 9853 Size: 11347 HaeII r_GCGC'y Cuts at: 1678 2253 3445 4447 4864 6475 6604 10494 1678 Size: 575 1192 1002 417 1611 129 3890 2531 Fragments arranged by size: 3890 2531 1611 1192 1002 575 417 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3312 3344 3381 3413 4236 Size: 32 135 32 1740 32 37 32 823 Cuts at: 4236 4268 4313 4345 4593 4625 4922 4954 5126 Size: 32 45 32 248 32 297 32 172 Cuts at: 5126 5158 6000 6032 7202 7234 8348 8380 9988 Size: 32 842 32 1170 32 1114 32 1608 Cuts at: 9988 10020 10062 10094 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1740 1608 1170 1114 842 823 297 248 172 135 45 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5704 6663 7150 8691 8937 669 Size: 988 281 3766 959 487 1541 246 3079 Fragments arranged by size: 3766 3079 1541 988 959 487 281 246 HindIII A'AGCT_T Cuts at: 748 4509 7294 748 Size: 3761 2785 4801 Fragments arranged by size: 4801 3761 2785 HpaI GTT'AAC Cuts at: 8937 8937 Size: 11347 KpnI G_GTAC'C Cuts at: 6328 7745 8672 6328 Size: 1417 927 9003 Fragments arranged by size: 9003 1417 927 MfeI C'AATT_G Cuts at: 3400 6517 7902 8946 3400 Size: 3117 1385 1044 5801 Fragments arranged by size: 5801 3117 1385 1044 MluI A'CGCG_T Cuts at: 11223 11223 Size: 11347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2676 4047 4443 4624 5634 6769 7971 8047 Size: 1371 1371 396 181 1010 1135 1202 76 Cuts at: 8047 8283 10461 10645 1305 Size: 236 2178 184 2007 Fragments arranged by size: 2178 2007 1371 1371 1202 1135 1010 396 236 184 181 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 4854 6141 10 Size: 54 1425 343 886 2136 1287 5216 Fragments arranged by size: 5216 2136 1425 1287 886 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3209 4784 4893 5021 5598 5772 Size: 648 1110 933 1575 109 128 577 174 Cuts at: 5772 6311 7041 7818 8163 9305 9664 9823 518 Size: 539 730 777 345 1142 359 159 2042 Fragments arranged by size: 2042 1575 1142 1110 933 777 730 648 577 539 359 345 174 159 128 109 MspA1I CmG'CkG Cuts at: 1606 2624 2695 3831 4537 5647 5733 6279 6698 Size: 1018 71 1136 706 1110 86 546 419 Cuts at: 6698 6979 8763 9440 10588 10833 1606 Size: 281 1784 677 1148 245 2120 Fragments arranged by size: 2120 1784 1148 1136 1110 1018 706 677 546 419 281 245 86 71 NaeI GCC'GGC Cuts at: 3196 3196 Size: 11347 NcoI C'CATG_G Cuts at: 513 1065 2004 5883 7042 7308 8634 513 Size: 552 939 3879 1159 266 1326 3226 Fragments arranged by size: 3879 3226 1326 1159 939 552 266 NdeI CA'TA_TG Cuts at: 387 6800 387 Size: 6413 4934 NgoMIV G'CCGG_C Cuts at: 3194 3194 Size: 11347 NheI G'CTAG_C Cuts at: 1052 3548 1052 Size: 2496 8851 NotI GC'GGCC_GC Cuts at: 8794 8794 Size: 11347 NruI TCG'CGA Cuts at: 2035 2035 Size: 11347 NsiI A_TGCA'T Cuts at: 8380 8380 Size: 11347 NspI r_CATG'y Cuts at: 1132 1229 2747 2926 3984 5512 7511 7823 8194 Size: 97 1518 179 1058 1528 1999 312 371 Cuts at: 8194 8705 10250 1132 Size: 511 1545 2229 Fragments arranged by size: 2229 1999 1545 1528 1518 1058 511 371 312 179 97 PasI CC'CwG_GG Cuts at: 1348 3415 3664 4638 6990 1348 Size: 2067 249 974 2352 5705 Fragments arranged by size: 5705 2352 2067 974 249 PciI A'CATG_T Cuts at: 2743 2922 8190 10246 2743 Size: 179 5268 2056 3844 Fragments arranged by size: 5268 3844 2056 179 PflMI CCAn_nnn'nTGG Cuts at: 1108 3253 3670 5884 6159 7095 1108 Size: 2145 417 2214 275 936 5360 Fragments arranged by size: 5360 2214 2145 936 417 275 PfoI T'CCnGG_A Cuts at: 3607 6615 7788 3607 Size: 3008 1173 7166 Fragments arranged by size: 7166 3008 1173 PmeI GTTT'AAAC Cuts at: 8651 8651 Size: 11347 PmlI CAC'GTG Cuts at: 6258 6258 Size: 11347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 9416 9448 10929 10961 4265 Size: 32 5119 32 1481 32 4651 Fragments arranged by size: 5119 4651 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2676 4977 5167 6291 6939 2676 Size: 2301 190 1124 648 7084 Fragments arranged by size: 7084 2301 1124 648 190 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11347 PsiI TTA'TAA Cuts at: 8917 8917 Size: 11347 PspOMI G'GGCC_C Cuts at: 6248 6248 Size: 11347 PstI C_TGCA'G Cuts at: 830 2262 5126 5261 5501 5555 6284 6653 7576 Size: 1432 2864 135 240 54 729 369 923 Cuts at: 7576 8502 8699 830 Size: 926 197 3478 Fragments arranged by size: 3478 2864 1432 926 923 729 369 240 197 135 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11347 PvuII CAG'CTG Cuts at: 2624 2695 3831 4537 6698 6979 2624 Size: 71 1136 706 2161 281 6992 Fragments arranged by size: 6992 2161 1136 706 281 71 SacI G_AGCT'C Cuts at: 2083 2083 Size: 11347 SalI G'TCGA_C Cuts at: 1936 8689 1936 Size: 6753 4594 SanDI GG'GwC_CC Cuts at: 4977 4977 Size: 11347 SapI GCTCTTCn'nnn_ Cuts at: 3776 7655 3776 Size: 3879 7468 SbfI CC_TGCA'GG Cuts at: 8699 8699 Size: 11347 ScaI AGT'ACT Cuts at: 9595 11307 9595 Size: 1712 9635 SexAI A'CCwGG_T Cuts at: 3075 4746 3075 Size: 1671 9676 SfcI C'TryA_G Cuts at: 826 1045 2258 5122 5257 5497 5551 6280 6649 Size: 219 1213 2864 135 240 54 729 369 Cuts at: 6649 6778 7572 8498 8533 8695 9830 10511 10702 Size: 129 794 926 35 162 1135 681 191 Cuts at: 10702 826 Size: 1471 Fragments arranged by size: 2864 1471 1213 1135 926 794 729 681 369 240 219 191 162 135 129 54 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11347 SmaI CCC'GGG Cuts at: 1872 2864 4040 8674 1872 Size: 992 1176 4634 4545 Fragments arranged by size: 4634 4545 1176 992 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 5201 5423 7547 9451 10352 Size: 197 926 942 2316 222 2124 1904 901 Cuts at: 10352 10614 820 Size: 262 1553 Fragments arranged by size: 2316 2124 1904 1553 942 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 9138 493 Size: 8645 2702 SpeI A'CTAG_T Cuts at: 152 7184 152 Size: 7032 4315 SphI G_CATG'C Cuts at: 1132 8705 1132 Size: 7573 3774 SspI AAT'ATT Cuts at: 5 52 3050 9271 5 Size: 47 2998 6221 2081 Fragments arranged by size: 6221 2998 2081 47 StuI AGG'CCT Cuts at: 1517 2648 3510 5606 6581 6647 1517 Size: 1131 862 2096 975 66 6217 Fragments arranged by size: 6217 2096 1131 975 862 66 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 3159 3207 3492 3864 4428 Size: 552 939 357 798 48 285 372 564 Cuts at: 4428 5100 5883 7042 7308 8224 8634 8786 513 Size: 672 783 1159 266 916 410 152 3074 Fragments arranged by size: 3074 1159 939 916 798 783 672 564 552 410 372 357 285 266 152 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3313 5381 6301 6416 6435 7055 7382 Size: 1255 1733 2068 920 115 19 620 327 Cuts at: 7382 9374 9391 9544 9729 10174 325 Size: 1992 17 153 185 445 1498 Fragments arranged by size: 2068 1992 1733 1498 1255 920 620 445 327 185 153 115 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2843 2919 3564 Size: 274 80 33 51 1443 866 76 645 Cuts at: 3564 5490 6669 6676 6883 7059 8478 9593 11305 Size: 1926 1179 7 207 176 1419 1115 1712 Cuts at: 11305 96 Size: 138 Fragments arranged by size: 1926 1712 1443 1419 1179 1115 866 645 274 207 176 138 80 76 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3019 3051 4037 4069 4751 4781 4783 4813 5159 Size: 32 986 32 682 30 2 30 346 Cuts at: 5159 5191 5834 5866 6460 6492 6548 6580 7245 Size: 32 643 32 594 32 56 32 665 Cuts at: 7245 7277 8522 8554 11307 11339 3019 Size: 32 1245 32 2753 32 3027 Fragments arranged by size: 3027 2753 1245 986 682 665 643 594 346 56 32 32 32 32 32 32 32 32 32 30 30 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11347 XbaI T'CTAG_A Cuts at: 8683 8683 Size: 11347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 3529 3862 5356 6002 7477 8266 2659 Size: 870 333 1494 646 1475 789 5740 Fragments arranged by size: 5740 1494 1475 870 789 646 333 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11347 XmaI C'CCGG_G Cuts at: 1870 2862 4038 8672 1870 Size: 992 1176 4634 4545 Fragments arranged by size: 4634 4545 1176 992 XmnI GAAnn'nnTTC Cuts at: 3722 4280 5030 7349 9476 3722 Size: 558 750 2319 2127 5593 Fragments arranged by size: 5593 2319 2127 750 558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4435 8629 9155 276 Size: 53 83 186 936 2901 4194 526 2468 Fragments arranged by size: 4194 2901 2468 936 526 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I FseI FspAI KasI NarI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI