Restriction map for
pFN21AB4406
(Circular) (Six-base) MAPSORT of: pFN21AB4406.seq Check: 7781 from: 1 to: 11347
pFN21AB4406 11347 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5115 7543 7824 5115
Size: 2428 281 8638
Fragments arranged by size:
8638 2428 281
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4437 8631 9157 278
Size: 53 83 186 936 2901 4194 526 2468
Fragments arranged by size:
4194 2901 2468 936 526 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3273 6774 8690 1937
Size: 1336 3501 1916 4594
Fragments arranged by size:
4594 3501 1916 1336
Acc65I G'GTAC_C
Cuts at: 6324 7741 8668 6324
Size: 1417 927 9003
Fragments arranged by size:
9003 1417 927
AclI AA'CG_TT
Cuts at: 1563 9474 9847 1563
Size: 7911 373 3063
Fragments arranged by size:
7911 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3164 3347 4718 6671 8285 9410 10794 11062
Size: 1532 183 1371 1953 1614 1125 1384 268
Cuts at: 11062 1632
Size: 1917
Fragments arranged by size:
1953 1917 1614 1532 1384 1371 1125 268
183
AfeI AGC'GCT
Cuts at: 2251 4862 6473 6602 2251
Size: 2611 1611 129 6996
Fragments arranged by size:
6996 2611 1611 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11150
AflIII A'CryG_T
Cuts at: 1203 2412 2743 2922 3561 6257 6452 8190 8283
Size: 1209 331 179 639 2696 195 1738 93
Cuts at: 8283 10246 11223 1203
Size: 1963 977 1327
Fragments arranged by size:
2696 1963 1738 1327 1209 977 639 331
195 179 93
AgeI A'CCGG_T
Cuts at: 10178 10178
Size: 11347
AhdI GACnn_n'nnGTC
Cuts at: 10076 10076
Size: 11347
AleI CACnn'nnGTG
Cuts at: 1166 3209 5598 6311 1166
Size: 2043 2389 713 6202
Fragments arranged by size:
6202 2389 2043 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4911 1184
Size: 3727 7620
AlwNI CAG_nnn'CTG
Cuts at: 2107 3773 5559 5872 8084 9090 10662 2107
Size: 1666 1786 313 2212 1006 1572 2792
Fragments arranged by size:
2792 2212 1786 1666 1572 1006 313
ApaI G_GGCC'C
Cuts at: 6252 6252
Size: 11347
ApaLI G'TGCA_C
Cuts at: 2324 9404 10560 2324
Size: 7080 1156 3111
Fragments arranged by size:
7080 3111 1156
ApoI r'AATT_y
Cuts at: 1493 2445 4115 8253 8656 8882 9034 11143 1493
Size: 952 1670 4138 403 226 152 2109 1697
Fragments arranged by size:
4138 2109 1697 1670 952 403 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3042 3291 9901 160
Size: 872 2010 249 6610 1606
Fragments arranged by size:
6610 2010 1606 872 249
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11347
AvaI C'yCGr_G
Cuts at: 1870 1943 2264 2367 2862 4038 4330 8672 1870
Size: 73 321 103 495 1176 292 4342 4545
Fragments arranged by size:
4545 4342 1176 495 321 292 103 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8177 8210 8177
Size: 33 11314
BamHI G'GATC_C
Cuts at: 5695 8677 9044 5695
Size: 2982 367 7998
Fragments arranged by size:
7998 2982 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6324 7741 8425 8668 10123
Size: 325 217 627 4537 1417 684 243 1455
Cuts at: 10123 618
Size: 1842
Fragments arranged by size:
4537 1842 1455 1417 684 627 325 243
217
BanII G_rGCy'C
Cuts at: 1391 2083 2271 4645 5016 5823 6252 7027 8666
Size: 692 188 2374 371 807 429 775 1639
Cuts at: 8666 1391
Size: 4072
Fragments arranged by size:
4072 2374 1639 807 775 692 429 371
188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2827 2961 3784 5231 6455 6812 928
Size: 971 928 134 823 1447 1224 357 5463
Fragments arranged by size:
5463 1447 1224 971 928 823 357 134
BbvCI CC'TCA_GC
Cuts at: 4529 4529
Size: 11347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2335 2369 5912 5946 9538 9572 1903
Size: 34 398 34 3543 34 3592 34 3678
Fragments arranged by size:
3678 3592 3543 398 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5408 5659 9239 10449 5408
Size: 251 3580 1210 6306
Fragments arranged by size:
6306 3580 1210 251
BclI T'GATC_A
Cuts at: 1548 4479 8113 1548
Size: 2931 3634 4782
Fragments arranged by size:
4782 3634 2931
BfrBI ATG'CAT
Cuts at: 8378 8378
Size: 11347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 5190 5742
Size: 107 122 71 631 605 535 2983 552
Cuts at: 5742 6501 8313 9958 136
Size: 759 1812 1645 1525
Fragments arranged by size:
2983 1812 1645 1525 759 631 605 552
535 122 107 71
BglII A'GATC_T
Cuts at: 7915 8322 8399 11342 7915
Size: 407 77 2943 7920
Fragments arranged by size:
7920 2943 407 77
BlpI GC'TnA_GC
Cuts at: 3438 4946 8450 8764 3438
Size: 1508 3504 314 6021
Fragments arranged by size:
6021 3504 1508 314
Bme1580I G_kGCm'C
Cuts at: 1790 2328 4659 5073 5634 6252 8430 9408 10564
Size: 538 2331 414 561 618 2178 978 1156
Cuts at: 10564 1790
Size: 2573
Fragments arranged by size:
2573 2331 2178 1156 978 618 561 538
414
BmgBI CAC'GTC
Cuts at: 1318 6453 7632 1318
Size: 5135 1179 5033
Fragments arranged by size:
5135 5033 1179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 2494 2551
Size: 416 53 83 399 24 921 156 57
Cuts at: 2551 3013 4555 4696 4936 5362 7030 8036 9078
Size: 462 1542 141 240 426 1668 1006 1042
Cuts at: 9078 10036 442
Size: 958 1753
Fragments arranged by size:
1753 1668 1542 1042 1006 958 921 462
426 416 399 240 156 141 83 57
53 24
BmtI G_CTAG'C
Cuts at: 1056 3552 1056
Size: 2496 8851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 3354 3449 3489 3603 3827 4517 4844
Size: 1116 999 95 40 114 224 690 327
Cuts at: 4844 6833 6959 7225 8524 10007 1239
Size: 1989 126 266 1299 1483 2579
Fragments arranged by size:
2579 1989 1483 1299 1116 999 690 327
266 224 126 114 95 40
Bpu10I CC'TnA_GC
Cuts at: 4529 4529
Size: 11347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 5186 5444 7532 9472 10337 10635 2906
Size: 2280 258 2088 1940 865 298 3618
Fragments arranged by size:
3618 2280 2088 1940 865 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2992 3627 3704 4686 6809 10010 882
Size: 617 1493 635 77 982 2123 3201 2219
Fragments arranged by size:
3201 2219 2123 1493 982 635 617 77
BsaAI yAC'GTr
Cuts at: 493 1204 2413 6258 6870 9138 493
Size: 711 1209 3845 612 2268 2702
Fragments arranged by size:
3845 2702 2268 1209 711 612
BsaBI GATnn'nnATC
Cuts at: 5694 11341 5694
Size: 5647 5700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4434 8628 9154 9536
Size: 53 83 186 936 2901 4194 526 382
Cuts at: 9536 10171 275
Size: 635 1451
Fragments arranged by size:
4194 2901 1451 936 635 526 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2235 3471 4111 4188 8490 9780 10178 10452 10599
Size: 1236 640 77 4302 1290 398 274 147
Cuts at: 10599 11010 2235
Size: 411 2572
Fragments arranged by size:
4302 2572 1290 1236 640 411 398 274
147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4209 4236 4239 4266 6548
Size: 30 165 30 2806 27 3 27 2282
Cuts at: 6548 6578 8522 8552 1178
Size: 30 1944 30 3973
Fragments arranged by size:
3973 2806 2282 1944 165 30 30 30
30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 3323 3446 3752 4233 5512 7017 7396
Size: 1435 702 123 306 481 1279 1505 379
Cuts at: 7396 8554 1186
Size: 1158 3979
Fragments arranged by size:
3979 1505 1435 1279 1158 702 481 379
306 123
BseYI C'CCAG_C
Cuts at: 3937 5643 8359 8545 10550 11046 3937
Size: 1706 2716 186 2005 496 4238
Fragments arranged by size:
4238 2716 2005 1706 496 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 2177 3862 4018 6357 7521 8391
Size: 573 357 84 1685 156 2339 1164 870
Cuts at: 8391 1163
Size: 4119
Fragments arranged by size:
4119 2339 1685 1164 870 573 357 156
84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8797 9558 10586 1540
Size: 275 186 6796 761 1028 2301
Fragments arranged by size:
6796 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2328 3174 3788 4401 6594 9408 9493 10564
Size: 245 846 614 613 2193 2814 85 1071
Cuts at: 10564 2083
Size: 2866
Fragments arranged by size:
2866 2814 2193 1071 846 614 613 245
85
BsmI GAATG_Cn'
Cuts at: 3483 6440 8858 8951 3483
Size: 2957 2418 93 5879
Fragments arranged by size:
5879 2957 2418 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2271 2328 3174 3788 4401 4645
Size: 399 293 188 57 846 614 613 244
Cuts at: 4645 4659 5016 5073 5634 5823 6252 6594 7027
Size: 14 357 57 561 189 429 342 433
Cuts at: 7027 8430 8666 9408 9493 10564 1391
Size: 1403 236 742 85 1071 2174
Fragments arranged by size:
2174 1403 1071 846 742 614 613 561
433 429 399 357 342 293 244 236
189 188 85 57 57 14
BspEI T'CCGG_A
Cuts at: 2235 3471 4111 11010 2235
Size: 1236 640 6899 2572
Fragments arranged by size:
6899 2572 1236 640
BspHI T'CATG_A
Cuts at: 2730 9236 10966 2730
Size: 6506 1730 3111
Fragments arranged by size:
6506 3111 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5115 6302 7543 7824 8702 844
Size: 4271 1187 1241 281 878 3489
Fragments arranged by size:
4271 3489 1241 1187 878 281
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3919 4837 5520 9234 1394
Size: 429 410 1686 918 683 3714 3507
Fragments arranged by size:
3714 3507 1686 918 683 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 3276 3657 4899 5065 5728 9842 10016
Size: 1183 2028 381 1242 166 663 4114 174
Cuts at: 10016 65
Size: 1396
Fragments arranged by size:
4114 2028 1396 1242 1183 663 381 174
166
BsrFI r'CCGG_y
Cuts at: 1700 1810 3194 4009 7592 9991 10178 1700
Size: 110 1384 815 3583 2399 187 2869
Fragments arranged by size:
3583 2869 2399 1384 815 187 110
BsrGI T'GTAC_A
Cuts at: 96 3564 6669 8478 96
Size: 3468 3105 1809 2965
Fragments arranged by size:
3468 3105 2965 1809
BssHII G'CGCG_C
Cuts at: 1925 2121 2189 1925
Size: 196 68 11083
Fragments arranged by size:
11083 196 68
BssSI C'ACGA_G
Cuts at: 2150 4652 9407 10419 2150
Size: 2502 4755 1012 3078
Fragments arranged by size:
4755 3078 2502 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2107 8087 794
Size: 1313 5980 4054
Fragments arranged by size:
5980 4054 1313
BstBI TT'CG_AA
Cuts at: 6432 10151 6432
Size: 3719 7628
BstEII G'GTnAC_C
Cuts at: 1184 3903 1184
Size: 2719 8628
BstXI CCAn_nnnn'nTGG
Cuts at: 4645 5387 7043 8327 4645
Size: 742 1656 1284 7665
Fragments arranged by size:
7665 1656 1284 742
BstYI r'GATC_y
Cuts at: 1972 4108 5695 5956 7915 8322 8399 8677 9044
Size: 2136 1587 261 1959 407 77 278 367
Cuts at: 9044 9429 9446 10898 10984 10996 11342 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2136 1977 1959 1587 1452 407 385 367
346 278 261 86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 5187 6935 5187
Size: 1748 9599
BtgI C'CryG_G
Cuts at: 513 1065 2004 3637 3855 5883 7042 7308 7845
Size: 552 939 1633 218 2028 1159 266 537
Cuts at: 7845 8634 513
Size: 789 3226
Fragments arranged by size:
3226 2028 1633 1159 939 789 552 537
266 218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 3502 5340 6427 6651 487
Size: 683 56 1814 462 1838 1087 224 5183
Fragments arranged by size:
5183 1838 1814 1087 683 462 224 56
BtsI GCAGTG_nn'
Cuts at: 2063 2407 2962 3205 3583 4380 5155 5632 5812
Size: 344 555 243 378 797 775 477 180
Cuts at: 5812 5873 6142 6292 6381 6658 7581 8863 9657
Size: 61 269 150 89 277 923 1282 794
Cuts at: 9657 9677 2063
Size: 20 3733
Fragments arranged by size:
3733 1282 923 797 794 775 555 477
378 344 277 269 243 180 150 89
61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4954 4989 5293 5328 6388 6423 6473
Size: 35 4390 35 304 35 1060 35 50
Cuts at: 6473 6508 529
Size: 35 5368
Fragments arranged by size:
5368 4390 1060 304 50 35 35 35
35 35
DraI TTT'AAA
Cuts at: 8148 8251 8651 8148
Size: 103 400 10844
Fragments arranged by size:
10844 400 103
DraIII CAC_nnn'GTG
Cuts at: 3253 4750 3253
Size: 1497 9850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2292 10354 809
Size: 507 976 8062 1802
Fragments arranged by size:
8062 1802 976 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 2995 3654
Size: 54 1425 50 275 18 886 279 659
Cuts at: 3654 4852 6139 6481 6502 8794 9683 11312 8
Size: 1198 1287 342 21 2292 889 1629 43
Fragments arranged by size:
2292 1629 1425 1287 1198 889 886 659
342 279 275 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 8794 1537
Size: 275 6982 4090
Fragments arranged by size:
6982 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2598 3776 4266 5220 7655 9277 1353
Size: 1245 1178 490 954 2435 1622 3423
Fragments arranged by size:
3423 2435 1622 1245 1178 954 490
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3657 3979 9934 10308 10454 387
Size: 1064 138 2068 322 5955 374 146 1280
Fragments arranged by size:
5955 2068 1280 1064 374 322 146 138
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 11347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 3164 3347 3354 3449 3489 3603
Size: 393 723 809 183 7 95 40 114
Cuts at: 3603 3827 4517 4718 4844 6671 6833 6959 7225
Size: 224 690 201 126 1827 162 126 266
Cuts at: 7225 8285 8524 9410 10007 10794 11062 1239
Size: 1060 239 886 597 787 268 1524
Fragments arranged by size:
1827 1524 1060 886 809 787 723 690
597 393 268 266 239 224 201 183
162 126 126 114 95 40 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3211 3491 5099 6730 7105 3211
Size: 280 1608 1631 375 7453
Fragments arranged by size:
7453 1631 1608 375 280
EcoO109I rG'GnC_Cy
Cuts at: 2676 4977 5167 6249 6291 6939 2676
Size: 2301 190 1082 42 648 7084
Fragments arranged by size:
7084 2301 1082 648 190 42
EcoRI G'AATT_C
Cuts at: 8656 9034 8656
Size: 378 10969
EcoRV GAT'ATC
Cuts at: 7261 7321 7261
Size: 60 11287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2900 7495 8160 2479
Size: 421 4595 665 5666
Fragments arranged by size:
5666 4595 665 421
FspI TGC'GCA
Cuts at: 9853 9853
Size: 11347
HaeII r_GCGC'y
Cuts at: 1678 2253 3445 4447 4864 6475 6604 10494 1678
Size: 575 1192 1002 417 1611 129 3890 2531
Fragments arranged by size:
3890 2531 1611 1192 1002 575 417 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3312 3344 3381 3413 4236
Size: 32 135 32 1740 32 37 32 823
Cuts at: 4236 4268 4313 4345 4593 4625 4922 4954 5126
Size: 32 45 32 248 32 297 32 172
Cuts at: 5126 5158 6000 6032 7202 7234 8348 8380 9988
Size: 32 842 32 1170 32 1114 32 1608
Cuts at: 9988 10020 10062 10094 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1740 1608 1170 1114 842 823 297
248 172 135 45 42 37 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5704 6663 7150 8691 8937 669
Size: 988 281 3766 959 487 1541 246 3079
Fragments arranged by size:
3766 3079 1541 988 959 487 281 246
HindIII A'AGCT_T
Cuts at: 748 4509 7294 748
Size: 3761 2785 4801
Fragments arranged by size:
4801 3761 2785
HpaI GTT'AAC
Cuts at: 8937 8937
Size: 11347
KpnI G_GTAC'C
Cuts at: 6328 7745 8672 6328
Size: 1417 927 9003
Fragments arranged by size:
9003 1417 927
MfeI C'AATT_G
Cuts at: 3400 6517 7902 8946 3400
Size: 3117 1385 1044 5801
Fragments arranged by size:
5801 3117 1385 1044
MluI A'CGCG_T
Cuts at: 11223 11223
Size: 11347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2676 4047 4443 4624 5634 6769 7971 8047
Size: 1371 1371 396 181 1010 1135 1202 76
Cuts at: 8047 8283 10461 10645 1305
Size: 236 2178 184 2007
Fragments arranged by size:
2178 2007 1371 1371 1202 1135 1010 396
236 184 181 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2718 4854 6141 10
Size: 54 1425 343 886 2136 1287 5216
Fragments arranged by size:
5216 2136 1425 1287 886 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3209 4784 4893 5021 5598 5772
Size: 648 1110 933 1575 109 128 577 174
Cuts at: 5772 6311 7041 7818 8163 9305 9664 9823 518
Size: 539 730 777 345 1142 359 159 2042
Fragments arranged by size:
2042 1575 1142 1110 933 777 730 648
577 539 359 345 174 159 128 109
MspA1I CmG'CkG
Cuts at: 1606 2624 2695 3831 4537 5647 5733 6279 6698
Size: 1018 71 1136 706 1110 86 546 419
Cuts at: 6698 6979 8763 9440 10588 10833 1606
Size: 281 1784 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 1136 1110 1018 706 677
546 419 281 245 86 71
NaeI GCC'GGC
Cuts at: 3196 3196
Size: 11347
NcoI C'CATG_G
Cuts at: 513 1065 2004 5883 7042 7308 8634 513
Size: 552 939 3879 1159 266 1326 3226
Fragments arranged by size:
3879 3226 1326 1159 939 552 266
NdeI CA'TA_TG
Cuts at: 387 6800 387
Size: 6413 4934
NgoMIV G'CCGG_C
Cuts at: 3194 3194
Size: 11347
NheI G'CTAG_C
Cuts at: 1052 3548 1052
Size: 2496 8851
NotI GC'GGCC_GC
Cuts at: 8794 8794
Size: 11347
NruI TCG'CGA
Cuts at: 2035 2035
Size: 11347
NsiI A_TGCA'T
Cuts at: 8380 8380
Size: 11347
NspI r_CATG'y
Cuts at: 1132 1229 2747 2926 3984 5512 7511 7823 8194
Size: 97 1518 179 1058 1528 1999 312 371
Cuts at: 8194 8705 10250 1132
Size: 511 1545 2229
Fragments arranged by size:
2229 1999 1545 1528 1518 1058 511 371
312 179 97
PasI CC'CwG_GG
Cuts at: 1348 3415 3664 4638 6990 1348
Size: 2067 249 974 2352 5705
Fragments arranged by size:
5705 2352 2067 974 249
PciI A'CATG_T
Cuts at: 2743 2922 8190 10246 2743
Size: 179 5268 2056 3844
Fragments arranged by size:
5268 3844 2056 179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3253 3670 5884 6159 7095 1108
Size: 2145 417 2214 275 936 5360
Fragments arranged by size:
5360 2214 2145 936 417 275
PfoI T'CCnGG_A
Cuts at: 3607 6615 7788 3607
Size: 3008 1173 7166
Fragments arranged by size:
7166 3008 1173
PmeI GTTT'AAAC
Cuts at: 8651 8651
Size: 11347
PmlI CAC'GTG
Cuts at: 6258 6258
Size: 11347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 9416 9448 10929 10961 4265
Size: 32 5119 32 1481 32 4651
Fragments arranged by size:
5119 4651 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2676 4977 5167 6291 6939 2676
Size: 2301 190 1124 648 7084
Fragments arranged by size:
7084 2301 1124 648 190
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11347
PsiI TTA'TAA
Cuts at: 8917 8917
Size: 11347
PspOMI G'GGCC_C
Cuts at: 6248 6248
Size: 11347
PstI C_TGCA'G
Cuts at: 830 2262 5126 5261 5501 5555 6284 6653 7576
Size: 1432 2864 135 240 54 729 369 923
Cuts at: 7576 8502 8699 830
Size: 926 197 3478
Fragments arranged by size:
3478 2864 1432 926 923 729 369 240
197 135 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11347
PvuII CAG'CTG
Cuts at: 2624 2695 3831 4537 6698 6979 2624
Size: 71 1136 706 2161 281 6992
Fragments arranged by size:
6992 2161 1136 706 281 71
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 11347
SalI G'TCGA_C
Cuts at: 1936 8689 1936
Size: 6753 4594
SanDI GG'GwC_CC
Cuts at: 4977 4977
Size: 11347
SapI GCTCTTCn'nnn_
Cuts at: 3776 7655 3776
Size: 3879 7468
SbfI CC_TGCA'GG
Cuts at: 8699 8699
Size: 11347
ScaI AGT'ACT
Cuts at: 9595 11307 9595
Size: 1712 9635
SexAI A'CCwGG_T
Cuts at: 3075 4746 3075
Size: 1671 9676
SfcI C'TryA_G
Cuts at: 826 1045 2258 5122 5257 5497 5551 6280 6649
Size: 219 1213 2864 135 240 54 729 369
Cuts at: 6649 6778 7572 8498 8533 8695 9830 10511 10702
Size: 129 794 926 35 162 1135 681 191
Cuts at: 10702 826
Size: 1471
Fragments arranged by size:
2864 1471 1213 1135 926 794 729 681
369 240 219 191 162 135 129 54
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11347
SmaI CCC'GGG
Cuts at: 1872 2864 4040 8674 1872
Size: 992 1176 4634 4545
Fragments arranged by size:
4634 4545 1176 992
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2885 5201 5423 7547 9451 10352
Size: 197 926 942 2316 222 2124 1904 901
Cuts at: 10352 10614 820
Size: 262 1553
Fragments arranged by size:
2316 2124 1904 1553 942 926 901 262
222 197
SnaBI TAC'GTA
Cuts at: 493 9138 493
Size: 8645 2702
SpeI A'CTAG_T
Cuts at: 152 7184 152
Size: 7032 4315
SphI G_CATG'C
Cuts at: 1132 8705 1132
Size: 7573 3774
SspI AAT'ATT
Cuts at: 5 52 3050 9271 5
Size: 47 2998 6221 2081
Fragments arranged by size:
6221 2998 2081 47
StuI AGG'CCT
Cuts at: 1517 2648 3510 5606 6581 6647 1517
Size: 1131 862 2096 975 66 6217
Fragments arranged by size:
6217 2096 1131 975 862 66
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 3159 3207 3492 3864 4428
Size: 552 939 357 798 48 285 372 564
Cuts at: 4428 5100 5883 7042 7308 8224 8634 8786 513
Size: 672 783 1159 266 916 410 152 3074
Fragments arranged by size:
3074 1159 939 916 798 783 672 564
552 410 372 357 285 266 152 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3313 5381 6301 6416 6435 7055 7382
Size: 1255 1733 2068 920 115 19 620 327
Cuts at: 7382 9374 9391 9544 9729 10174 325
Size: 1992 17 153 185 445 1498
Fragments arranged by size:
2068 1992 1733 1498 1255 920 620 445
327 185 153 115 19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2843 2919 3564
Size: 274 80 33 51 1443 866 76 645
Cuts at: 3564 5490 6669 6676 6883 7059 8478 9593 11305
Size: 1926 1179 7 207 176 1419 1115 1712
Cuts at: 11305 96
Size: 138
Fragments arranged by size:
1926 1712 1443 1419 1179 1115 866 645
274 207 176 138 80 76 51 33
7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3019 3051 4037 4069 4751 4781 4783 4813 5159
Size: 32 986 32 682 30 2 30 346
Cuts at: 5159 5191 5834 5866 6460 6492 6548 6580 7245
Size: 32 643 32 594 32 56 32 665
Cuts at: 7245 7277 8522 8554 11307 11339 3019
Size: 32 1245 32 2753 32 3027
Fragments arranged by size:
3027 2753 1245 986 682 665 643 594
346 56 32 32 32 32 32 32
32 32 32 30 30 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11347
XbaI T'CTAG_A
Cuts at: 8683 8683
Size: 11347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 3529 3862 5356 6002 7477 8266 2659
Size: 870 333 1494 646 1475 789 5740
Fragments arranged by size:
5740 1494 1475 870 789 646 333
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11347
XmaI C'CCGG_G
Cuts at: 1870 2862 4038 8672 1870
Size: 992 1176 4634 4545
Fragments arranged by size:
4634 4545 1176 992
XmnI GAAnn'nnTTC
Cuts at: 3722 4280 5030 7349 9476 3722
Size: 558 750 2319 2127 5593
Fragments arranged by size:
5593 2319 2127 750 558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4435 8629 9155 276
Size: 53 83 186 936 2901 4194 526 2468
Fragments arranged by size:
4194 2901 2468 936 526 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I
FseI FspAI KasI NarI PacI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI