Restriction map for
pFN21AB4401
(Circular) (Six-base) MAPSORT of: pFN21AB4401.seq Check: 6473 from: 1 to: 5314
pFN21AB4401 5314 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3124 278
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2657 1937
Size: 720 4594
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 2613 3441 3814 1563
Size: 1050 828 373 3063
Fragments arranged by size:
3063 1050 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 3377 4761 5029 1632
Size: 804 941 1384 268 1917
Fragments arranged by size:
1917 1384 941 804 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 2439 4213 5190 1203
Size: 1236 1774 977 1327
Fragments arranged by size:
1774 1327 1236 977
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 2537
Size: 32 5282
AlwNI CAG_nnn'CTG
Cuts at: 3057 4629 3057
Size: 1572 3742
ApaLI G'TGCA_C
Cuts at: 3371 4527 3371
Size: 1156 4158
ApoI r'AATT_y
Cuts at: 1493 2420 2623 2849 3001 5110 1493
Size: 927 203 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 2163 3868 160
Size: 872 1131 1705 1606
Fragments arranged by size:
1705 1606 1131 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 1870
Size: 73 696 4545
Fragments arranged by size:
4545 696 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 4090 618
Size: 325 217 627 848 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2633 1391
Size: 1242 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 928
Size: 971 176 4167
Fragments arranged by size:
4167 971 176
BbvCI CC'TCA_GC
Cuts at: 2458 2458
Size: 5314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2563 3206 4416 2563
Size: 643 1210 3461
Fragments arranged by size:
3461 1210 643
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2187 5309 2187
Size: 3122 2192
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 3375 4531 1790
Size: 1585 1156 2573
Fragments arranged by size:
2573 1585 1156
BmgBI CAC'GTC
Cuts at: 1318 2073 1318
Size: 755 4559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 2589 3974 1239
Size: 1232 118 1385 2579
Fragments arranged by size:
2579 1385 1232 118
Bpu10I CC'TnA_GC
Cuts at: 2433 2458 2433
Size: 25 5289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3439 4304 4602 3439
Size: 865 298 4151
Fragments arranged by size:
4151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 3977 882
Size: 617 948 1530 2219
Fragments arranged by size:
2219 1530 948 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 493
Size: 711 1901 2702
Fragments arranged by size:
2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3121 3503 4138 275
Size: 53 83 186 936 1588 382 635 1451
Fragments arranged by size:
1588 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3747 4145 4419 4566 4977 3747
Size: 398 274 147 411 4084
Fragments arranged by size:
4084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5089
Fragments arranged by size:
5089 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5314
BseYI C'CCAG_C
Cuts at: 2403 4517 5013 2403
Size: 2114 496 2704
Fragments arranged by size:
2704 2114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4741
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3375 3460 4531 3375
Size: 85 1071 4158
Fragments arranged by size:
4158 1071 85
BsmI GAATG_Cn'
Cuts at: 2825 2918 2825
Size: 93 5221
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2633 3375 3460 4531 1391
Size: 399 843 742 85 1071 2174
Fragments arranged by size:
2174 1071 843 742 399 85
BspEI T'CCGG_A
Cuts at: 4977 4977
Size: 5314
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2669 844
Size: 1348 477 3489
Fragments arranged by size:
3489 1348 477
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3958 4145 1700
Size: 110 2148 187 2869
Fragments arranged by size:
2869 2148 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5314
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5314
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2187 2644 3011 3396 3413 4865 4951 4963
Size: 215 457 367 385 17 1452 86 12
Cuts at: 4963 5309 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 457 385 367 346 215 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4575
Fragments arranged by size:
4575 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 809
Size: 507 3005 1802
Fragments arranged by size:
3005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2761 3650 5279
Size: 54 1425 50 275 18 931 889 1629
Cuts at: 5279 8
Size: 43
Fragments arranged by size:
1629 1425 931 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 3244 1353
Size: 1142 749 3423
Fragments arranged by size:
3423 1142 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3901 4275 4421 387
Size: 1064 138 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 2471 2589 3377 3974 4761 5029
Size: 393 804 35 118 788 597 787 268
Cuts at: 5029 1239
Size: 1524
Fragments arranged by size:
1524 804 788 787 597 393 268 118
35
EcoNI CCTnn'n_nnAGG
Cuts at: 2571 2571
Size: 5314
EcoRI G'AATT_C
Cuts at: 2420 2623 3001 2420
Size: 203 378 4733
Fragments arranged by size:
4733 378 203
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2250 4461 1678
Size: 572 2211 2531
Fragments arranged by size:
2531 2211 572
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3955 3987 4029 4061 1373
Size: 32 135 32 2383 32 42 32 2626
Fragments arranged by size:
2626 2383 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2127 2913 2127
Size: 786 4528
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4428 4612 1305
Size: 3123 184 2007
Fragments arranged by size:
3123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3492
Fragments arranged by size:
3492 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2354 2540 3272 3631 3790 518
Size: 648 843 345 186 732 359 159 2042
Fragments arranged by size:
2042 843 732 648 359 345 186 159
MspA1I CmG'CkG
Cuts at: 1606 2730 3407 4555 4800 1606
Size: 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4762
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2443 2508 2672 4217 1132
Size: 97 1214 65 164 1545 2229
Fragments arranged by size:
2229 1545 1214 164 97 65
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 2439 4213 2439
Size: 1774 3540
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PstI C_TGCA'G
Cuts at: 830 2666 830
Size: 1836 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SfcI C'TryA_G
Cuts at: 826 1045 2183 2662 3797 4478 4669 826
Size: 219 1138 479 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 479 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2641 1872
Size: 769 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3418 4319 4581 820
Size: 197 926 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2508 2672 1132
Size: 1376 164 3774
Fragments arranged by size:
3774 1376 164
SspI AAT'ATT
Cuts at: 5 52 3238 5
Size: 47 3186 2081
Fragments arranged by size:
3186 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2463 2753 513
Size: 552 1398 290 3074
Fragments arranged by size:
3074 1398 552 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2208 3341 3358 3511 3696 4141 325
Size: 1255 628 1133 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1133 628 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3560 5272 96
Size: 274 80 33 51 1443 1583 1712 138
Fragments arranged by size:
1712 1583 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5274 5306 5274
Size: 32 5282
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5314
XmaI C'CCGG_G
Cuts at: 1870 2639 1870
Size: 769 4545
XmnI GAAnn'nnTTC
Cuts at: 2489 3443 2489
Size: 954 4360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3122 276
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI