Restriction map for
pFN21AB4394
(Circular) (Six-base) MAPSORT of: pFN21AB4394.seq Check: 6913 from: 1 to: 7207
pFN21AB4394 7207 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5017 278
Size: 53 83 186 936 3481 2468
Fragments arranged by size:
3481 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4167 4550 1937
Size: 2230 383 4594
Fragments arranged by size:
4594 2230 383
Acc65I G'GTAC_C
Cuts at: 2916 4528 2916
Size: 1612 5595
AclI AA'CG_TT
Cuts at: 1563 5334 5707 1563
Size: 3771 373 3063
Fragments arranged by size:
3771 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 3781 3807 4159 4340 5270 6654 6922
Size: 483 1666 26 352 181 930 1384 268
Cuts at: 6922 1632
Size: 1917
Fragments arranged by size:
1917 1666 1384 930 483 352 268 181
26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7010
AflIII A'CryG_T
Cuts at: 1203 3523 6106 7083 1203
Size: 2320 2583 977 1327
Fragments arranged by size:
2583 2320 1327 977
AgeI A'CCGG_T
Cuts at: 6038 6038
Size: 7207
AhdI GACnn_n'nnGTC
Cuts at: 5936 5936
Size: 7207
AleI CACnn'nnGTG
Cuts at: 1166 2470 4299 1166
Size: 1304 1829 4074
Fragments arranged by size:
4074 1829 1304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2775 4209 1184
Size: 1591 1434 4182
Fragments arranged by size:
4182 1591 1434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 3749 3781 2141
Size: 32 1576 32 5567
Fragments arranged by size:
5567 1576 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2167 2743 3103 3221 4210 4396 4950 6522 2167
Size: 576 360 118 989 186 554 1572 2852
Fragments arranged by size:
2852 1572 989 576 554 360 186 118
ApaI G_GGCC'C
Cuts at: 3931 4148 3931
Size: 217 6990
ApaLI G'TGCA_C
Cuts at: 2389 4290 5264 6420 2389
Size: 1901 974 1156 3176
Fragments arranged by size:
3176 1901 1156 974
ApoI r'AATT_y
Cuts at: 1493 3956 4516 4742 4894 7003 1493
Size: 2463 560 226 152 2109 1697
Fragments arranged by size:
2463 2109 1697 560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5761 160
Size: 872 4729 1606
Fragments arranged by size:
4729 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 3576 3973 4016 4130 4141 4233
Size: 73 138 1495 397 43 114 11 92
Cuts at: 4233 4532 1870
Size: 299 4545
Fragments arranged by size:
4545 1495 397 299 138 114 92 73
43 11
AvrII C'CTAG_G
Cuts at: 2293 2293
Size: 7207
BamHI G'GATC_C
Cuts at: 4537 4904 4537
Size: 367 6840
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 2346 2543 2916 3503
Size: 325 217 627 494 65 197 373 587
Cuts at: 3503 4400 4528 5983 618
Size: 897 128 1455 1842
Fragments arranged by size:
1842 1455 897 627 587 494 373 325
217 197 128 65
BanII G_rGCy'C
Cuts at: 1391 2067 2140 2679 2881 3096 3374 3452 3863
Size: 676 73 539 202 215 278 78 411
Cuts at: 3863 3931 4148 4526 1391
Size: 68 217 378 4072
Fragments arranged by size:
4072 676 539 411 378 278 217 215
202 78 73 68
BbeI G_GCGC'C
Cuts at: 2285 2350 3507 2285
Size: 65 1157 5985
Fragments arranged by size:
5985 1157 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3463 3555 3684 928
Size: 971 1564 92 129 4451
Fragments arranged by size:
4451 1564 971 129 92
BbvCI CC'TCA_GC
Cuts at: 3161 3848 4239 3161
Size: 687 391 6129
Fragments arranged by size:
6129 687 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2194 2228 3641 3675 5398 5432 1903
Size: 34 257 34 1413 34 1723 34 3678
Fragments arranged by size:
3678 1723 1413 257 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3208 5099 6309 3208
Size: 1891 1210 4106
Fragments arranged by size:
4106 1891 1210
BclI T'GATC_A
Cuts at: 1548 3534 1548
Size: 1986 5221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2808 3512 4103
Size: 107 122 71 631 605 1136 704 591
Cuts at: 4103 5818 136
Size: 1715 1525
Fragments arranged by size:
1715 1525 1136 704 631 605 591 122
107 71
BglII A'GATC_T
Cuts at: 7202 7202
Size: 7207
BlpI GC'TnA_GC
Cuts at: 4624 4624
Size: 7207
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2270 2393 3314 3931 4017 4148 4294
Size: 414 66 123 921 617 86 131 146
Cuts at: 4294 4403 5268 6424 1790
Size: 109 865 1156 2573
Fragments arranged by size:
2573 1156 921 865 617 414 146 131
123 109 86 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3301 4938 5896
Size: 416 53 83 399 24 1884 1637 958
Cuts at: 5896 442
Size: 1753
Fragments arranged by size:
1884 1753 1637 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 3988 4510 2107
Size: 1881 522 4804
Fragments arranged by size:
4804 1881 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2918 3599 3845 3953 5867 1239
Size: 1028 651 681 246 108 1914 2579
Fragments arranged by size:
2579 1914 1028 681 651 246 108
Bpu10I CC'TnA_GC
Cuts at: 2236 2880 3161 3848 4239 2236
Size: 644 281 687 391 5204
Fragments arranged by size:
5204 687 644 391 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2663 3689 4022 5332 6197 6495 2465
Size: 198 1026 333 1310 865 298 3177
Fragments arranged by size:
3177 1310 1026 865 333 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2819 4141 5870 882
Size: 617 579 741 1322 1729 2219
Fragments arranged by size:
2219 1729 1322 741 617 579
BsaAI yAC'GTr
Cuts at: 493 1204 2872 3493 4998 493
Size: 711 1668 621 1505 2702
Fragments arranged by size:
2702 1668 1505 711 621
BsaBI GATnn'nnATC
Cuts at: 7201 7201
Size: 7207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 2282 2347 2727
Size: 53 83 186 936 719 30 65 380
Cuts at: 2727 3504 5014 5396 6031 275
Size: 777 1510 382 635 1451
Fragments arranged by size:
1510 1451 936 777 719 635 382 380
186 83 65 53 30
BsaWI w'CCGG_w
Cuts at: 5640 6038 6312 6459 6870 5640
Size: 398 274 147 411 5977
Fragments arranged by size:
5977 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2059 2089 2141 2147 2171
Size: 30 165 30 656 30 52 6 24
Cuts at: 2171 2177 3344 3374 3557 3587 1178
Size: 6 1167 30 183 30 4798
Fragments arranged by size:
4798 1167 656 183 165 52 30 30
30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2168 2432 2732 2846 2948 3104 3356
Size: 979 3 264 300 114 102 156 252
Cuts at: 3356 4118 1186
Size: 762 4275
Fragments arranged by size:
4275 979 762 300 264 252 156 114
102 3
BseYI C'CCAG_C
Cuts at: 2037 2108 2114 2120 2806 2950 3392 3629 4149
Size: 71 6 6 686 144 442 237 520
Cuts at: 4149 4158 4371 4497 6410 6906 2037
Size: 9 213 126 1913 496 2338
Fragments arranged by size:
2338 1913 686 520 496 442 237 213
144 126 71 9 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1998 2207 2319 3023 3316 4235 1163
Size: 573 262 209 112 704 293 919 4135
Fragments arranged by size:
4135 919 704 573 293 262 209 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2412 2796 4657 5418 6446 1540
Size: 275 186 411 384 1861 761 1028 2301
Fragments arranged by size:
2301 1861 1028 761 411 384 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2393 2679 3374 3863 4294 5268 5353 6424
Size: 156 286 695 489 431 974 85 1071
Cuts at: 6424 2237
Size: 3020
Fragments arranged by size:
3020 1071 974 695 489 431 286 156
85
BsmI GAATG_Cn'
Cuts at: 4718 4811 4718
Size: 93 7114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2140 2204 2237 2270 2393 2679
Size: 399 277 73 64 33 33 123 286
Cuts at: 2679 2881 3096 3314 3374 3452 3863 3931 4017
Size: 202 215 218 60 78 411 68 86
Cuts at: 4017 4148 4294 4403 4526 5268 5353 6424 1391
Size: 131 146 109 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 286 277 218
215 202 146 131 123 123 109 86
85 78 73 68 64 60 33 33
BspEI T'CCGG_A
Cuts at: 6870 6870
Size: 7207
BspHI T'CATG_A
Cuts at: 5096 6826 5096
Size: 1730 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4395 4562 844
Size: 3551 167 3489
Fragments arranged by size:
3551 3489 167
BsrBI CCG'CTC
Cuts at: 1394 1823 5094 1394
Size: 429 3271 3507
Fragments arranged by size:
3507 3271 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2186 5702 5876 65
Size: 1183 938 3516 174 1396
Fragments arranged by size:
3516 1396 1183 938 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2361 4305 5851 6038 1700
Size: 110 551 1944 1546 187 2869
Fragments arranged by size:
2869 1944 1546 551 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7207
BssHII G'CGCG_C
Cuts at: 1925 2649 2814 2994 1925
Size: 724 165 180 6138
Fragments arranged by size:
6138 724 180 165
BssSI C'ACGA_G
Cuts at: 2486 2644 5267 6279 2486
Size: 158 2623 1012 3414
Fragments arranged by size:
3414 2623 1012 158
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2265 2397 2613 2743 794
Size: 1471 132 216 130 5258
Fragments arranged by size:
5258 1471 216 132 130
BstBI TT'CG_AA
Cuts at: 6011 6011
Size: 7207
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7207
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 3428 2950
Size: 478 6729
BstYI r'GATC_y
Cuts at: 1972 2700 4537 4904 5289 5306 6758 6844 6856
Size: 728 1837 367 385 17 1452 86 12
Cuts at: 6856 7202 1972
Size: 346 1977
Fragments arranged by size:
1977 1837 1452 728 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3638 3638
Size: 7207
BtgI C'CryG_G
Cuts at: 513 1065 2004 4089 4474 513
Size: 552 939 2085 385 3246
Fragments arranged by size:
3246 2085 939 552 385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6468
Fragments arranged by size:
6468 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 3604 4193 4723 5517 5537 2270
Size: 1334 589 530 794 20 3940
Fragments arranged by size:
3940 1334 794 589 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4447 4482 529
Size: 35 3883 35 3254
Fragments arranged by size:
3883 3254 35 35
DraI TTT'AAA
Cuts at: 4511 4511
Size: 7207
DraIII CAC_nnn'GTG
Cuts at: 2232 4267 2232
Size: 2035 5172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3260 6214 809
Size: 507 1944 2954 1802
Fragments arranged by size:
2954 1944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 2793 2800
Size: 54 1425 50 275 18 579 384 7
Cuts at: 2800 2940 3495 4104 4471 4654 5543 7172 8
Size: 140 555 609 367 183 889 1629 43
Fragments arranged by size:
1629 1425 889 609 579 555 384 367
275 183 140 54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2409 2793 4654 1537
Size: 275 597 384 1861 4090
Fragments arranged by size:
4090 1861 597 384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3281 5137 1353
Size: 1928 1856 3423
Fragments arranged by size:
3423 1928 1856
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 5794 6168 6314 387
Size: 1064 138 615 3590 374 146 1280
Fragments arranged by size:
3590 1280 1064 615 374 146 138
EcoICRI GAG'CTC
Cuts at: 2677 3372 3861 2677
Size: 695 489 6023
Fragments arranged by size:
6023 695 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2267 2918 3599 3781 3807 3845
Size: 393 483 152 651 681 182 26 38
Cuts at: 3845 3953 4159 4340 5270 5867 6654 6922 1239
Size: 108 206 181 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 681 651 597 483 393
268 206 182 181 152 108 38 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3062 3083 4130 4214 4421 3062
Size: 21 1047 84 207 5848
Fragments arranged by size:
5848 1047 207 84 21
EcoO109I rG'GnC_Cy
Cuts at: 2034 2044 3157 3238 3626 3643 3902 3928 4145
Size: 10 1113 81 388 17 259 26 217
Cuts at: 4145 4315 4491 2034
Size: 170 176 4750
Fragments arranged by size:
4750 1113 388 259 217 176 170 81
26 17 10
EcoRI G'AATT_C
Cuts at: 3956 4516 4894 3956
Size: 560 378 6269
Fragments arranged by size:
6269 560 378
FspI TGC'GCA
Cuts at: 2379 2513 3119 5713 2379
Size: 134 606 2594 3873
Fragments arranged by size:
3873 2594 606 134
FspAI rTGC'GCAy
Cuts at: 3119 3119
Size: 7207
HaeII r_GCGC'y
Cuts at: 1678 2285 2350 2744 3071 3507 4089 4436 6354
Size: 607 65 394 327 436 582 347 1918
Cuts at: 6354 1678
Size: 2531
Fragments arranged by size:
2531 1918 607 582 436 394 347 327
65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2929 2961 3149 3181 5848
Size: 32 135 32 1357 32 188 32 2667
Cuts at: 5848 5880 5922 5954 1373
Size: 32 42 32 2626
Fragments arranged by size:
2667 2626 1357 188 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3253 4551 4797 669
Size: 988 281 1315 1298 246 3079
Fragments arranged by size:
3079 1315 1298 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7207
HpaI GTT'AAC
Cuts at: 4797 4797
Size: 7207
KasI G'GCGC_C
Cuts at: 2281 2346 3503 2281
Size: 65 1157 5985
Fragments arranged by size:
5985 1157 65
KpnI G_GTAC'C
Cuts at: 2920 4532 2920
Size: 1612 5595
MfeI C'AATT_G
Cuts at: 4806 4806
Size: 7207
MluI A'CGCG_T
Cuts at: 3523 7083 3523
Size: 3560 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 4401 6321 6505 1305
Size: 1293 1803 1920 184 2007
Fragments arranged by size:
2007 1920 1803 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2942 3497 4106 10
Size: 54 1425 343 1110 555 609 3111
Fragments arranged by size:
3111 1425 1110 609 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2470 3329 3426 4299 5165 5524 5683
Size: 648 1304 859 97 873 866 359 159
Cuts at: 5683 518
Size: 2042
Fragments arranged by size:
2042 1304 873 866 859 648 359 159
97
MspA1I CmG'CkG
Cuts at: 1606 2344 2397 2635 2806 3194 3619 3739 4153
Size: 738 53 238 171 388 425 120 414
Cuts at: 4153 4223 4623 5300 6448 6693 1606
Size: 70 400 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 425 414 400 388
245 238 171 120 70 53
NarI GG'CG_CC
Cuts at: 2282 2347 3504 2282
Size: 65 1157 5985
Fragments arranged by size:
5985 1157 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 4089 513
Size: 552 939 2085 3631
Fragments arranged by size:
3631 2085 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7207
NotI GC'GGCC_GC
Cuts at: 4654 4654
Size: 7207
NspI r_CATG'y
Cuts at: 1132 1229 2263 3049 4166 4565 6110 1132
Size: 97 1034 786 1117 399 1545 2229
Fragments arranged by size:
2229 1545 1117 1034 786 399 97
PasI CC'CwG_GG
Cuts at: 1348 3178 3234 1348
Size: 1830 56 5321
Fragments arranged by size:
5321 1830 56
PciI A'CATG_T
Cuts at: 6106 6106
Size: 7207
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4469 1108
Size: 3361 3846
PfoI T'CCnGG_A
Cuts at: 2535 3645 3960 4002 2535
Size: 1110 315 42 5740
Fragments arranged by size:
5740 1110 315 42
PmeI GTTT'AAAC
Cuts at: 4511 4511
Size: 7207
PmlI CAC'GTG
Cuts at: 2872 3493 2872
Size: 621 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 5276 5308 6789 6821 2141
Size: 32 3103 32 1481 32 2527
Fragments arranged by size:
3103 2527 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 3157 3238 3643 2044
Size: 1113 81 405 5608
Fragments arranged by size:
5608 1113 405 81
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7207
PsiI TTA'TAA
Cuts at: 4777 4777
Size: 7207
PspOMI G'GGCC_C
Cuts at: 3927 4144 3927
Size: 217 6990
PspXI vC'TCGA_Gb
Cuts at: 4130 4130
Size: 7207
PstI C_TGCA'G
Cuts at: 830 2343 2748 2976 3156 3840 4188 4206 4350
Size: 1513 405 228 180 684 348 18 144
Cuts at: 4350 4392 4559 830
Size: 42 167 3478
Fragments arranged by size:
3478 1513 684 405 348 228 180 167
144 42 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7207
PvuII CAG'CTG
Cuts at: 2806 3194 3619 3739 4153 4223 2806
Size: 388 425 120 414 70 5790
Fragments arranged by size:
5790 425 414 388 120 70
SacI G_AGCT'C
Cuts at: 2679 3374 3863 2679
Size: 695 489 6023
Fragments arranged by size:
6023 695 489
SalI G'TCGA_C
Cuts at: 1936 4549 1936
Size: 2613 4594
SanDI GG'GwC_CC
Cuts at: 3238 3238
Size: 7207
SbfI CC_TGCA'GG
Cuts at: 3156 4392 4559 3156
Size: 1236 167 5804
Fragments arranged by size:
5804 1236 167
ScaI AGT'ACT
Cuts at: 5455 7167 5455
Size: 1712 5495
SfcI C'TryA_G
Cuts at: 826 1045 2127 2339 2744 2972 3152 3836 4184
Size: 219 1082 212 405 228 180 684 348
Cuts at: 4184 4202 4346 4388 4555 5690 6371 6562 826
Size: 18 144 42 167 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 684 681 405 348 228
219 212 191 180 167 144 42 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2808 2808
Size: 7207
SfoI GGC'GCC
Cuts at: 2283 2348 3505 2283
Size: 65 1157 5985
Fragments arranged by size:
5985 1157 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7207
SmaI CCC'GGG
Cuts at: 1872 4018 4143 4235 4534 1872
Size: 2146 125 92 299 4545
Fragments arranged by size:
4545 2146 299 125 92
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2678 3704 4037 4130 5311
Size: 197 926 537 198 1026 333 93 1181
Cuts at: 5311 6212 6474 820
Size: 901 262 1553
Fragments arranged by size:
1553 1181 1026 926 901 537 333 262
198 197 93
SnaBI TAC'GTA
Cuts at: 493 4998 493
Size: 4505 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7207
SphI G_CATG'C
Cuts at: 1132 2263 4565 1132
Size: 1131 2302 3774
Fragments arranged by size:
3774 2302 1131
SspI AAT'ATT
Cuts at: 5 52 5131 5
Size: 47 5079 2081
Fragments arranged by size:
5079 2081 47
StuI AGG'CCT
Cuts at: 1517 4416 1517
Size: 2899 4308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2293 3402 3810 4089 4646 513
Size: 552 939 289 1109 408 279 557 3074
Fragments arranged by size:
3074 1109 939 557 552 408 289 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2843 3191 5234 5251 5404 5589 6034
Size: 1255 1263 348 2043 17 153 185 445
Cuts at: 6034 325
Size: 1498
Fragments arranged by size:
2043 1498 1263 1255 445 348 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4326 5453 7165
Size: 274 80 33 51 1443 2349 1127 1712
Cuts at: 7165 96
Size: 138
Fragments arranged by size:
2349 1712 1443 1127 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2059 2091 2183 2215 7167 7199 2059
Size: 32 92 32 4952 32 2067
Fragments arranged by size:
4952 2067 92 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7207
XbaI T'CTAG_A
Cuts at: 4543 4543
Size: 7207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3497 3497
Size: 7207
XhoI C'TCGA_G
Cuts at: 1943 4130 1943
Size: 2187 5020
XmaI C'CCGG_G
Cuts at: 1870 4016 4141 4233 4532 1870
Size: 2146 125 92 299 4545
Fragments arranged by size:
4545 2146 299 125 92
XmnI GAAnn'nnTTC
Cuts at: 5336 5336
Size: 7207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5015 276
Size: 53 83 186 936 3481 2468
Fragments arranged by size:
3481 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI
PsrI RsrII SacII SapI SexAI SrfI SwaI