Restriction map for
pFN21AB4393
(Circular) (Six-base) MAPSORT of: pFN21AB4393.seq Check: 7127 from: 1 to: 8875
pFN21AB4393 8875 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5162 5162
Size: 8875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6685 278
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4071 4581 6218 1937
Size: 2134 510 1637 4594
Fragments arranged by size:
4594 2134 1637 510
Acc65I G'GTAC_C
Cuts at: 2495 6196 2495
Size: 3701 5174
AclI AA'CG_TT
Cuts at: 1563 7002 7375 1563
Size: 5439 373 3063
Fragments arranged by size:
5439 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2281 2399 2570 2721 3059 3445 3790 4154
Size: 649 118 171 151 338 386 345 364
Cuts at: 4154 4283 5174 6938 8322 8590 1632
Size: 129 891 1764 1384 268 1917
Fragments arranged by size:
1917 1764 1384 891 649 386 364 345
338 268 171 151 129 118
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8678
AflIII A'CryG_T
Cuts at: 1203 7774 8751 1203
Size: 6571 977 1327
Fragments arranged by size:
6571 1327 977
AgeI A'CCGG_T
Cuts at: 7706 7706
Size: 8875
AhdI GACnn_n'nnGTC
Cuts at: 7604 7604
Size: 8875
AleI CACnn'nnGTG
Cuts at: 1166 5828 1166
Size: 4662 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8875
AlwNI CAG_nnn'CTG
Cuts at: 2380 4302 5096 5578 6618 8190 2380
Size: 1922 794 482 1040 1572 3065
Fragments arranged by size:
3065 1922 1572 1040 794 482
ApaLI G'TGCA_C
Cuts at: 6932 8088 6932
Size: 1156 7719
ApoI r'AATT_y
Cuts at: 1493 2578 2677 3168 4916 6152 6184 6410 6562
Size: 1085 99 491 1748 1236 32 226 152
Cuts at: 6562 8671 1493
Size: 2109 1697
Fragments arranged by size:
2109 1748 1697 1236 1085 491 226 152
99 32
AseI AT'TA_AT
Cuts at: 160 1032 7429 160
Size: 872 6397 1606
Fragments arranged by size:
6397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
AvaI C'yCGr_G
Cuts at: 1870 1943 2980 3598 4425 6200 1870
Size: 73 1037 618 827 1775 4545
Fragments arranged by size:
4545 1775 1037 827 618 73
AvrII C'CTAG_G
Cuts at: 3967 3967
Size: 8875
BamHI G'GATC_C
Cuts at: 2563 3977 6205 6572 2563
Size: 1414 2228 367 4866
Fragments arranged by size:
4866 2228 1414 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2773 2822 3001 3647
Size: 325 217 627 708 278 49 179 646
Cuts at: 3647 5138 5732 6196 7651 618
Size: 1491 594 464 1455 1842
Fragments arranged by size:
1842 1491 1455 708 646 627 594 464
325 278 217 179 49
BanII G_rGCy'C
Cuts at: 1391 2979 3336 4995 5555 6081 6194 1391
Size: 1588 357 1659 560 526 113 4072
Fragments arranged by size:
4072 1659 1588 560 526 357 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4789 5162 5978 928
Size: 971 2890 373 816 3825
Fragments arranged by size:
3825 2890 971 816 373
BbvCI CC'TCA_GC
Cuts at: 4236 4236
Size: 8875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5830 5864 7066 7100 1903
Size: 34 3893 34 1202 34 3678
Fragments arranged by size:
3893 3678 1202 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 6767 7977 4604
Size: 2163 1210 5502
Fragments arranged by size:
5502 2163 1210
BclI T'GATC_A
Cuts at: 1548 2919 1548
Size: 1371 7504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4061 7486 136
Size: 107 122 71 631 605 2389 3425 1525
Fragments arranged by size:
3425 2389 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3801 3892 4645 8870 3801
Size: 91 753 4225 3806
Fragments arranged by size:
4225 3806 753 91
BlpI GC'TnA_GC
Cuts at: 6292 6292
Size: 8875
Bme1580I G_kGCm'C
Cuts at: 1790 2647 2959 5080 5119 5208 5735 6936 8092
Size: 857 312 2121 39 89 527 1201 1156
Cuts at: 8092 1790
Size: 2573
Fragments arranged by size:
2573 2121 1201 1156 857 527 312 89
39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2941 4024 4651
Size: 416 53 83 399 24 1524 1083 627
Cuts at: 4651 4803 6606 7564 442
Size: 152 1803 958 1753
Fragments arranged by size:
1803 1753 1524 1083 958 627 416 399
152 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3086
Size: 8875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 4268 5069 7535 1239
Size: 1049 1980 801 2466 2579
Fragments arranged by size:
2579 2466 1980 1049 801
Bpu10I CC'TnA_GC
Cuts at: 4236 4236
Size: 8875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3235 3312 3320 3650 7000 7865 8163 3235
Size: 77 8 330 3350 865 298 3947
Fragments arranged by size:
3947 3350 865 330 298 77 8
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 4936 5045 7538 882
Size: 617 2736 701 109 2493 2219
Fragments arranged by size:
2736 2493 2219 701 617 109
BsaAI yAC'GTr
Cuts at: 493 1204 5566 5863 6666 493
Size: 711 4362 297 803 2702
Fragments arranged by size:
4362 2702 803 711 297
BsaBI GATnn'nnATC
Cuts at: 3834 8869 3834
Size: 5035 3840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6682 7064 7699 275
Size: 53 83 186 936 5149 382 635 1451
Fragments arranged by size:
5149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3129 3453 4523 7308 7706 7980 8127 8538 3129
Size: 324 1070 2785 398 274 147 411 3466
Fragments arranged by size:
3466 2785 1070 411 398 324 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 3366 3396 1178
Size: 30 165 30 858 30 1075 30 6657
Fragments arranged by size:
6657 1075 858 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2378 3647 4253 4728 1186
Size: 910 282 1269 606 475 5333
Fragments arranged by size:
5333 1269 910 606 475 282
BseYI C'CCAG_C
Cuts at: 2635 2695 4486 8078 8574 2635
Size: 60 1791 3592 496 2936
Fragments arranged by size:
3592 2936 1791 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 4213 5449 5627 1163
Size: 573 1656 821 1236 178 4411
Fragments arranged by size:
4411 1656 1236 821 573 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4567 6325 7086 8114 1540
Size: 275 186 2566 1758 761 1028 2301
Fragments arranged by size:
2566 2301 1758 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2979 3336 5110 5555 6936 7021 8092 2172
Size: 807 357 1774 445 1381 85 1071 2955
Fragments arranged by size:
2955 1774 1381 1071 807 445 357 85
BsmI GAATG_Cn'
Cuts at: 2985 4162 5613 6386 6479 2985
Size: 1177 1451 773 93 5381
Fragments arranged by size:
5381 1451 1177 773 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 8875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2647 2959 2979 3336 4995 5080
Size: 399 382 475 312 20 357 1659 85
Cuts at: 5080 5110 5119 5208 5555 5735 6081 6194 6936
Size: 30 9 89 347 180 346 113 742
Cuts at: 6936 7021 8092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1659 1071 742 475 399 382 357
347 346 312 180 113 89 85 85
30 20 9
BspEI T'CCGG_A
Cuts at: 3129 8538 3129
Size: 5409 3466
BspHI T'CATG_A
Cuts at: 2530 4860 5883 6764 8494 2530
Size: 2330 1023 881 1730 2911
Fragments arranged by size:
2911 2330 1730 1023 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 3861 5162 5706 6230 844
Size: 1501 1516 1301 544 524 3489
Fragments arranged by size:
3489 1516 1501 1301 544 524
BsrBI CCG'CTC
Cuts at: 1394 1823 6762 1394
Size: 429 4939 3507
Fragments arranged by size:
4939 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3705 7370 7544 65
Size: 1183 2457 3665 174 1396
Fragments arranged by size:
3665 2457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4160 4562 7519 7706 1700
Size: 110 2350 402 2957 187 2869
Fragments arranged by size:
2957 2869 2350 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 2127 3482 96
Size: 2031 1355 5489
Fragments arranged by size:
5489 2031 1355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8875
BssSI C'ACGA_G
Cuts at: 5303 6935 7947 5303
Size: 1632 1012 6231
Fragments arranged by size:
6231 1632 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2337 4302 5105 5866 794
Size: 1543 1965 803 761 3803
Fragments arranged by size:
3803 1965 1543 803 761
BstBI TT'CG_AA
Cuts at: 7679 7679
Size: 8875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8875
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 5126 5602 2480
Size: 2646 476 5753
Fragments arranged by size:
5753 2646 476
BstYI r'GATC_y
Cuts at: 1972 2563 2895 3801 3892 3977 4645 4726 5587
Size: 591 332 906 91 85 668 81 861
Cuts at: 5587 6205 6572 6957 6974 8426 8512 8524 8870
Size: 618 367 385 17 1452 86 12 346
Cuts at: 8870 1972
Size: 1977
Fragments arranged by size:
1977 1452 906 861 668 618 591 385
367 346 332 91 86 85 81 17
12
Bsu36I CC'TnA_GG
Cuts at: 2079 3240 3818 2079
Size: 1161 578 7136
Fragments arranged by size:
7136 1161 578
BtgI C'CryG_G
Cuts at: 513 1065 2004 4084 4341 5826 5836 513
Size: 552 939 2080 257 1485 10 3552
Fragments arranged by size:
3552 2080 1485 939 552 257 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3843 487
Size: 683 56 2617 5519
Fragments arranged by size:
5519 2617 683 56
BtsI GCAGTG_nn'
Cuts at: 2957 4230 4303 5106 6391 7185 7205 2957
Size: 1273 73 803 1285 794 20 4627
Fragments arranged by size:
4627 1285 1273 803 794 73 20
ClaI AT'CG_AT
Cuts at: 1554 3369 1554
Size: 1815 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8840
DraI TTT'AAA
Cuts at: 2686 4768 6179 2686
Size: 2082 1411 5382
Fragments arranged by size:
5382 2082 1411
DraIII CAC_nnn'GTG
Cuts at: 3786 5723 5725 3786
Size: 1937 2 6936
Fragments arranged by size:
6936 1937 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 7882 809
Size: 507 3536 3030 1802
Fragments arranged by size:
3536 3030 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 3162 3435
Size: 54 1425 50 275 18 207 1125 273
Cuts at: 3435 4120 4560 4564 5712 5928 6322 7211 8840
Size: 685 440 4 1148 216 394 889 1629
Cuts at: 8840 8
Size: 43
Fragments arranged by size:
1629 1425 1148 1125 889 685 440 394
275 273 216 207 54 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 4564 6322 1537
Size: 275 2752 1758 4090
Fragments arranged by size:
4090 2752 1758 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4862 6086 6805 1353
Size: 3509 1224 719 3423
Fragments arranged by size:
3509 3423 1224 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7462 7836 7982 387
Size: 1064 138 5873 374 146 1280
Fragments arranged by size:
5873 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2977 3334 5553 2977
Size: 357 2219 6299
Fragments arranged by size:
6299 2219 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2281 2288 2399 2570 2721 3059 3445
Size: 393 649 7 111 171 151 338 386
Cuts at: 3445 3790 4154 4268 4283 5069 5174 6938 7535
Size: 345 364 114 15 786 105 1764 597
Cuts at: 7535 8322 8590 1239
Size: 787 268 1524
Fragments arranged by size:
1764 1524 787 786 649 597 393 386
364 345 338 268 171 151 114 111
105 15 7
EcoNI CCTnn'n_nnAGG
Cuts at: 4122 5582 4122
Size: 1460 7415
EcoO109I rG'GnC_Cy
Cuts at: 3559 4452 6109 6129 3559
Size: 893 1657 20 6305
Fragments arranged by size:
6305 1657 893 20
EcoRI G'AATT_C
Cuts at: 6152 6184 6562 6152
Size: 32 378 8465
Fragments arranged by size:
8465 378 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3605 3820 5384 3605
Size: 215 1564 7096
Fragments arranged by size:
7096 1564 215
FseI GG_CCGG'CC
Cuts at: 4566 4566
Size: 8875
FspI TGC'GCA
Cuts at: 7381 7381
Size: 8875
HaeII r_GCGC'y
Cuts at: 1678 2487 8022 1678
Size: 809 5535 2531
Fragments arranged by size:
5535 2531 809
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 3012 3044 3351
Size: 32 135 32 1229 32 179 32 307
Cuts at: 3351 3383 4028 4060 4637 4669 5445 5477 5622
Size: 32 645 32 577 32 776 32 145
Cuts at: 5622 5654 7516 7548 7590 7622 1373
Size: 32 1862 32 42 32 2626
Fragments arranged by size:
2626 1862 1229 776 645 577 307 179
145 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4582 6219 6465 669
Size: 988 281 2644 1637 246 3079
Fragments arranged by size:
3079 2644 1637 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5675 748
Size: 4927 3948
HpaI GTT'AAC
Cuts at: 6465 6465
Size: 8875
KpnI G_GTAC'C
Cuts at: 2499 6200 2499
Size: 3701 5174
MfeI C'AATT_G
Cuts at: 4095 6474 4095
Size: 2379 6496
MluI A'CGCG_T
Cuts at: 8751 8751
Size: 8875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3055 5901 7989 8173 1305
Size: 1750 2846 2088 184 2007
Fragments arranged by size:
2846 2088 2007 1750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 3164 3437 4122 5714
Size: 54 1425 343 207 1125 273 685 1592
Cuts at: 5714 5930 10
Size: 216 2955
Fragments arranged by size:
2955 1592 1425 1125 685 343 273 216
207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 5144 5828 6047 6833 7192 7351
Size: 648 1312 2666 684 219 786 359 159
Cuts at: 7351 518
Size: 2042
Fragments arranged by size:
2666 2042 1312 786 684 648 359 219
159
MspA1I CmG'CkG
Cuts at: 1606 2699 2947 3616 4490 4601 5011 6291 6968
Size: 1093 248 669 874 111 410 1280 677
Cuts at: 6968 8116 8361 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1280 1148 1093 874 677 669 410
248 245 111
NaeI GCC'GGC
Cuts at: 4162 4564 4162
Size: 402 8473
NcoI C'CATG_G
Cuts at: 513 1065 2004 4341 5826 513
Size: 552 939 2337 1485 3562
Fragments arranged by size:
3562 2337 1485 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8875
NgoMIV G'CCGG_C
Cuts at: 4160 4562 4160
Size: 402 8473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8875
NotI GC'GGCC_GC
Cuts at: 6322 6322
Size: 8875
NspI r_CATG'y
Cuts at: 1132 1229 2150 4618 6233 7778 1132
Size: 97 921 2468 1615 1545 2229
Fragments arranged by size:
2468 2229 1615 1545 921 97
PasI CC'CwG_GG
Cuts at: 1348 5197 1348
Size: 3849 5026
PciI A'CATG_T
Cuts at: 7774 7774
Size: 8875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 3012 4558 1108
Size: 1074 830 1546 5425
Fragments arranged by size:
5425 1546 1074 830
PfoI T'CCnGG_A
Cuts at: 2022 4308 2022
Size: 2286 6589
PmeI GTTT'AAAC
Cuts at: 6179 6179
Size: 8875
PmlI CAC'GTG
Cuts at: 5863 5863
Size: 8875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6944 6976 8457 8489 6944
Size: 32 1481 32 7330
Fragments arranged by size:
7330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4452 6109 4452
Size: 1657 7218
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8875
PsiI TTA'TAA
Cuts at: 6445 6445
Size: 8875
PstI C_TGCA'G
Cuts at: 830 2946 2952 4036 4215 4735 4905 5101 5574
Size: 2116 6 1084 179 520 170 196 473
Cuts at: 5574 6227 830
Size: 653 3478
Fragments arranged by size:
3478 2116 1084 653 520 473 196 179
170 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8875
PvuII CAG'CTG
Cuts at: 2699 2947 4490 4601 5011 2699
Size: 248 1543 111 410 6563
Fragments arranged by size:
6563 1543 410 248 111
SacI G_AGCT'C
Cuts at: 2979 3336 5555 2979
Size: 357 2219 6299
Fragments arranged by size:
6299 2219 357
SalI G'TCGA_C
Cuts at: 1936 4580 6217 1936
Size: 2644 1637 4594
Fragments arranged by size:
4594 2644 1637
SanDI GG'GwC_CC
Cuts at: 6109 6109
Size: 8875
SapI GCTCTTCn'nnn_
Cuts at: 6086 6086
Size: 8875
SbfI CC_TGCA'GG
Cuts at: 4905 6227 4905
Size: 1322 7553
ScaI AGT'ACT
Cuts at: 3875 7123 8835 3875
Size: 3248 1712 3915
Fragments arranged by size:
3915 3248 1712
SfcI C'TryA_G
Cuts at: 826 1045 2942 2948 4032 4211 4731 4901 5056
Size: 219 1897 6 1084 179 520 170 155
Cuts at: 5056 5097 5570 6223 7358 8039 8230 826
Size: 41 473 653 1135 681 191 1471
Fragments arranged by size:
1897 1471 1135 1084 681 653 520 473
219 191 179 170 155 41 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8875
SmaI CCC'GGG
Cuts at: 1872 6202 1872
Size: 4330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3214 3327 3335 3665 4425 6979
Size: 197 926 1271 113 8 330 760 2554
Cuts at: 6979 7880 8142 820
Size: 901 262 1553
Fragments arranged by size:
2554 1553 1271 926 901 760 330 262
197 113 8
SnaBI TAC'GTA
Cuts at: 493 6666 493
Size: 6173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8875
SphI G_CATG'C
Cuts at: 1132 6233 1132
Size: 5101 3774
SspI AAT'ATT
Cuts at: 5 52 3991 6799 5
Size: 47 3939 2808 2081
Fragments arranged by size:
3939 2808 2081 47
StuI AGG'CCT
Cuts at: 1517 3823 1517
Size: 2306 6569
StyI C'CwwG_G
Cuts at: 513 1065 2004 3690 3861 3967 4123 4341 5235
Size: 552 939 1686 171 106 156 218 894
Cuts at: 5235 5595 5826 6314 513
Size: 360 231 488 3074
Fragments arranged by size:
3074 1686 939 894 552 488 360 231
218 171 156 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3019 3264 5045 5093 5156 6902 6919
Size: 1255 1439 245 1781 48 63 1746 17
Cuts at: 6919 7072 7257 7702 325
Size: 153 185 445 1498
Fragments arranged by size:
1781 1746 1498 1439 1255 445 245 185
153 63 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 3482 3873
Size: 274 80 33 51 1443 150 1355 391
Cuts at: 3873 4182 5067 5559 5670 5820 7121 8833 96
Size: 309 885 492 111 150 1301 1712 138
Fragments arranged by size:
1712 1443 1355 1301 885 492 391 309
274 150 150 138 111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 3069 3101 3366 3398 6008 6040 8835
Size: 32 776 32 265 32 2610 32 2795
Cuts at: 8835 8867 2261
Size: 32 2269
Fragments arranged by size:
2795 2610 2269 776 265 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8875
XbaI T'CTAG_A
Cuts at: 6211 6211
Size: 8875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2554 4501 4706 2060
Size: 494 1947 205 6229
Fragments arranged by size:
6229 1947 494 205
XhoI C'TCGA_G
Cuts at: 1943 4425 1943
Size: 2482 6393
XmaI C'CCGG_G
Cuts at: 1870 6200 1870
Size: 4330 4545
XmnI GAAnn'nnTTC
Cuts at: 2706 5043 5159 5364 7004 2706
Size: 2337 116 205 1640 4577
Fragments arranged by size:
4577 2337 1640 205 116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6683 276
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI