Restriction map for
pFN21AB4388
(Circular) (Six-base) MAPSORT of: pFN21AB4388.seq Check: 3872 from: 1 to: 5752
pFN21AB4388 5752 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 2683 3815 5199 5467 1632
Size: 728 323 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 728 323 268
AfeI AGC'GCT
Cuts at: 2750 2750
Size: 5752
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2898 3495 5067 2898
Size: 597 1572 3583
Fragments arranged by size:
3583 1572 597
ApaLI G'TGCA_C
Cuts at: 2169 3809 4965 2169
Size: 1640 1156 2956
Fragments arranged by size:
2956 1640 1156
ApoI r'AATT_y
Cuts at: 1493 3061 3287 3439 5548 1493
Size: 1568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3071 1391
Size: 1680 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2476 928
Size: 971 577 4204
Fragments arranged by size:
4204 971 577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2836 2870 3943 3977 1903
Size: 34 899 34 1073 34 3678
Fragments arranged by size:
3678 1073 899 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2173 3813 4969 1790
Size: 383 1640 1156 2573
Fragments arranged by size:
2573 1640 1156 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2515 2730 3483
Size: 416 53 83 399 24 1098 215 753
Cuts at: 3483 4441 442
Size: 958 1753
Fragments arranged by size:
1753 1098 958 753 416 399 215 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2455 4412 1239
Size: 1216 1957 2579
Fragments arranged by size:
2579 1957 1216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2025 5746 2025
Size: 3721 2031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 1178
Size: 30 165 30 1149 30 4348
Fragments arranged by size:
4348 1149 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2309 1186
Size: 1075 48 4629
Fragments arranged by size:
4629 1075 48
BseYI C'CCAG_C
Cuts at: 2608 4955 5451 2608
Size: 2347 496 2909
Fragments arranged by size:
2909 2347 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 1163
Size: 573 1014 4165
Fragments arranged by size:
4165 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2173 3813 3898 4969 2173
Size: 1640 85 1071 2956
Fragments arranged by size:
2956 1640 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
BsmBI CGTCTCn'nnnn_
Cuts at: 2353 2353
Size: 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 3071 3813 3898 4969 1391
Size: 399 383 898 742 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 399 383 85
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 4247 4421 65
Size: 1183 1140 1859 174 1396
Fragments arranged by size:
1859 1396 1183 1140 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 2770 4556 2770
Size: 1786 3966
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2318
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2352 2757 513
Size: 552 1287 405 3508
Fragments arranged by size:
3508 1287 552 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2027 2062 2860 2895 529
Size: 35 1463 35 798 35 3386
Fragments arranged by size:
3386 1463 798 35 35 35
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3199 4088
Size: 54 1425 50 275 18 1009 360 889
Cuts at: 4088 5717 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1009 889 360 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 3682 1353
Size: 983 1346 3423
Fragments arranged by size:
3423 1346 983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2455 2683 3815 4412 5199 5467
Size: 393 728 95 228 1132 597 787 268
Cuts at: 5467 1239
Size: 1524
Fragments arranged by size:
1524 1132 787 728 597 393 268 228
95
EcoO109I rG'GnC_Cy
Cuts at: 3029 3029
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
EcoRV GAT'ATC
Cuts at: 2522 2677 2522
Size: 155 5597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 5752
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2752 2956 4899 1678
Size: 1074 204 1943 2531
Fragments arranged by size:
2531 1943 1074 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3033 3065 4393 4425 4467
Size: 32 135 32 1461 32 1328 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 1461 1328 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2805 2949 4866 5050 1305
Size: 1500 144 1917 184 2007
Fragments arranged by size:
2007 1917 1500 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2841 10
Size: 54 1425 343 1009 2921
Fragments arranged by size:
2921 1425 1009 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2529 2591 3710 4069 4228 518
Size: 648 981 382 62 1119 359 159 2042
Fragments arranged by size:
2042 1119 981 648 382 359 159 62
MspA1I CmG'CkG
Cuts at: 1606 3168 3845 4993 5238 1606
Size: 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2352 2757 513
Size: 552 1287 405 3508
Fragments arranged by size:
3508 1287 552 405
NdeI CA'TA_TG
Cuts at: 387 2221 387
Size: 1834 3918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2215 1108
Size: 1107 4645
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2480 1613
Size: 867 4885
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2319 2351 2319
Size: 32 5720
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2336 2336
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2994 3100 4235 4916 5107 826
Size: 219 1949 106 1135 681 191 1471
Fragments arranged by size:
1949 1471 1135 681 219 191 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 2777 152
Size: 2625 3127
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2735 1517
Size: 1218 4534
StyI C'CwwG_G
Cuts at: 513 1065 2016 2352 2757 3191 513
Size: 552 951 336 405 434 3074
Fragments arranged by size:
3074 951 552 434 405 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2972 3998 5710
Size: 274 80 33 51 1443 995 1026 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1712 1443 1026 995 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 5712 5744 2552
Size: 32 3128 32 2560
Fragments arranged by size:
3128 2560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2359
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI