Restriction map for
pFN21AB4380
(Circular) (Six-base) MAPSORT of: pFN21AB4380.seq Check: 7346 from: 1 to: 5878
pFN21AB4380 5878 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3161 3161
Size: 5878
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AfeI AGC'GCT
Cuts at: 2795 2999 2795
Size: 204 5674
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2424 2886 4777 5754 1203
Size: 1221 462 1891 977 1327
Fragments arranged by size:
1891 1327 1221 977 462
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 5846
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaI G_GGCC'C
Cuts at: 2093 2093
Size: 5878
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3187 3413 3565 5674 1493
Size: 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2093 3203 1870
Size: 73 113 37 1110 4545
Fragments arranged by size:
4545 1110 113 73 37
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3149 3199 4654 618
Size: 325 217 627 231 1131 50 1455 1842
Fragments arranged by size:
1842 1455 1131 627 325 231 217 50
BanII G_rGCy'C
Cuts at: 1391 2093 2649 3197 1391
Size: 702 556 548 4072
Fragments arranged by size:
4072 702 556 548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 2578 3108 3130 928
Size: 971 380 299 530 22 3676
Fragments arranged by size:
3676 971 530 380 299 22
BbvCI CC'TCA_GC
Cuts at: 3023 3023
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2771 4489 136
Size: 107 122 71 631 605 1099 1718 1525
Fragments arranged by size:
1718 1525 1099 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 2330 2405 2456 2895 3295 2330
Size: 75 51 439 400 4913
Fragments arranged by size:
4913 439 400 75 51
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2340 3939 5095 1790
Size: 303 247 1599 1156 2573
Fragments arranged by size:
2573 1599 1156 303 247
BmgBI CAC'GTC
Cuts at: 1318 3159 1318
Size: 1841 4037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3143 4538 1239
Size: 1904 1395 2579
Fragments arranged by size:
2579 1904 1395
Bpu10I CC'TnA_GC
Cuts at: 3023 3023
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 2753 3062 4003 4868 5166 2176
Size: 577 309 941 865 298 2888
Fragments arranged by size:
2888 941 865 577 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 2473 2734 2869 3669 493
Size: 711 1044 225 261 135 800 2702
Fragments arranged by size:
2702 1044 800 711 261 225 135
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2907 3135 3685 4067
Size: 53 83 186 936 1374 228 550 382
Cuts at: 4067 4702 275
Size: 635 1451
Fragments arranged by size:
1451 1374 936 635 550 382 228 186
83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2029 2135 3062 1186
Size: 831 12 106 927 4002
Fragments arranged by size:
4002 927 831 106 12
BseYI C'CCAG_C
Cuts at: 2051 5081 5577 2051
Size: 3030 496 2352
Fragments arranged by size:
3030 2352 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 3139 1163
Size: 573 1158 245 3902
Fragments arranged by size:
3902 1158 573 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2457 2739 3939 4024 5095 2406
Size: 51 282 1200 85 1071 3189
Fragments arranged by size:
3189 1200 1071 282 85 51
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2340 2406 2457 2649 2739 3197
Size: 399 303 247 66 51 192 90 458
Cuts at: 3197 3939 4024 5095 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 303 247 192
90 85 66 51
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 3233 844
Size: 2317 72 3489
Fragments arranged by size:
3489 2317 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2651 3765 1394
Size: 429 828 1114 3507
Fragments arranged by size:
3507 1114 828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2307 2742 4373 4547 65
Size: 1183 1059 435 1631 174 1396
Fragments arranged by size:
1631 1396 1183 1059 435 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 2511 2774 3052 4522 4709 1700
Size: 110 289 412 263 278 1470 187 2869
Fragments arranged by size:
2869 1470 412 289 278 263 187 110
BsrGI T'GTAC_A
Cuts at: 96 2679 96
Size: 2583 3295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2399 3938 4950 2399
Size: 1539 1012 3327
Fragments arranged by size:
3327 1539 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 3654
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873
Size: 1236 367 385 17 1452 86 12 346
Cuts at: 5873 1972
Size: 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2202 2478 2520 2598 2709 2910 3042
Size: 552 1137 276 42 78 111 201 132
Cuts at: 3042 513
Size: 3349
Fragments arranged by size:
3349 1137 552 276 201 132 111 78
42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 4188 4208 3394
Size: 794 20 5064
Fragments arranged by size:
5064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2304 2475
Size: 54 1425 50 275 18 447 27 171
Cuts at: 2475 2523 2601 3325 4214 5843 8
Size: 48 78 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 447 275 171 78
54 50 48 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3143 3941 4538 5325 5593 1239
Size: 393 1511 798 597 787 268 1524
Fragments arranged by size:
1524 1511 798 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2089 2090 2151 2089
Size: 1 61 5816
Fragments arranged by size:
5816 61 1
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 5878
FspI TGC'GCA
Cuts at: 2756 4384 2756
Size: 1628 4250
FspAI rTGC'GCAy
Cuts at: 2756 2756
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2106 2178 2797 3001 5025 1678
Size: 428 72 619 204 2024 2531
Fragments arranged by size:
2531 2024 619 428 204 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373
Size: 32 135 32 2947 32 42 32 2626
Fragments arranged by size:
2947 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2422 2884 3058 3222 3468 669
Size: 988 281 484 462 174 164 246 3079
Fragments arranged by size:
3079 988 484 462 281 246 174 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3031 3133 4992 5176 1305
Size: 1726 102 1859 184 2007
Fragments arranged by size:
2007 1859 1726 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2525 10
Size: 54 1425 343 474 219 3363
Fragments arranged by size:
3363 1425 474 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2636 2816 3836 4195 4354 518
Size: 648 1080 390 180 1020 359 159 2042
Fragments arranged by size:
2042 1080 1020 648 390 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2600 2878 2923 2952 3027 3044 3294
Size: 458 536 278 45 29 75 17 250
Cuts at: 3294 3971 5119 5364 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 458 278 250 245
75 45 29 17
NaeI GCC'GGC
Cuts at: 2101 2513 2776 2101
Size: 412 263 5203
Fragments arranged by size:
5203 412 263
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5326
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NgoMIV G'CCGG_C
Cuts at: 2099 2511 2774 2099
Size: 412 263 5203
Fragments arranged by size:
5203 412 263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2428 2635 3236 4781 1132
Size: 97 1199 207 601 1545 2229
Fragments arranged by size:
2229 1545 1199 601 207 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 2424 4777 2424
Size: 2353 3525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 3161 3161
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2151
Size: 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 2089 2089
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2064 2878 2923 2952 3027 2064
Size: 814 45 29 75 4915
Fragments arranged by size:
4915 814 75 45 29
SacII CC_GC'GG
Cuts at: 2601 3045 2601
Size: 444 5434
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3091 3226 4361 5042 5233 826
Size: 219 2046 135 1135 681 191 1471
Fragments arranged by size:
2046 1471 1135 681 219 191 135
SfiI GGCCn_nnn'nGGCC
Cuts at: 2771 2771
Size: 5878
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 2058 2095 3205 1872
Size: 186 37 1110 4545
Fragments arranged by size:
4545 1110 186 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 2768 3077 3982 4883 5145
Size: 197 926 212 613 309 905 901 262
Cuts at: 5145 820
Size: 1553
Fragments arranged by size:
1553 926 905 901 613 309 262 212
197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SrfI GCCC'GGGC
Cuts at: 2058 2058
Size: 5878
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2526 3317 513
Size: 552 1461 791 3074
Fragments arranged by size:
3074 1461 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2539 2917 3905 3922 4075 4260 4705
Size: 1255 959 378 988 17 153 185 445
Cuts at: 4705 325
Size: 1498
Fragments arranged by size:
1498 1255 988 959 445 378 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 4124 5836
Size: 274 80 33 51 1443 702 1445 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1712 1445 1443 702 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 1316
Size: 1101 4777
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 2056 2093 3203 1870
Size: 186 37 1110 4545
Fragments arranged by size:
4545 1110 186 37
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfoI SwaI XcmI