Restriction map for
pFN21AB4374
(Circular) (Six-base) MAPSORT of: pFN21AB4374.seq Check: 2483 from: 1 to: 5947
pFN21AB4374 5947 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 3290 1937
Size: 849 504 4594
Fragments arranged by size:
4594 849 504
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4010 5394 5662 1632
Size: 2378 1384 268 1917
Fragments arranged by size:
2378 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2277 2846 2889 3007 3242 3256 3482 3634
Size: 784 569 43 118 235 14 226 152
Cuts at: 3634 5743 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 784 569 235 226 152 118
43 14
AseI AT'TA_AT
Cuts at: 160 1032 2403 2457 4501 160
Size: 872 1371 54 2044 1606
Fragments arranged by size:
2044 1606 1371 872 54
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2931 928
Size: 971 1032 3944
Fragments arranged by size:
3944 1032 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2873 2907 4138 4172 1903
Size: 34 936 34 1231 34 3678
Fragments arranged by size:
3678 1231 936 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 2880 3364 2880
Size: 484 5463
Bme1580I G_kGCm'C
Cuts at: 1790 2268 4008 5164 1790
Size: 478 1740 1156 2573
Fragments arranged by size:
2573 1740 1156 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2384 2587 3678
Size: 416 53 83 399 24 967 203 1091
Cuts at: 3678 4636 442
Size: 958 1753
Fragments arranged by size:
1753 1091 967 958 416 399 203 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3068 1056
Size: 2012 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4607 1239
Size: 3368 2579
Bpu10I CC'TnA_GC
Cuts at: 2726 2726
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2305 4610 882
Size: 617 806 2305 2219
Fragments arranged by size:
2305 2219 806 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 3113 3143 1178
Size: 30 165 30 645 30 1035 30 3982
Fragments arranged by size:
3982 1035 645 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3085 3242 1186
Size: 1899 157 3891
Fragments arranged by size:
3891 1899 157
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2507 1163
Size: 573 483 288 4603
Fragments arranged by size:
4603 573 483 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4008 4093 5164 4008
Size: 85 1071 4791
Fragments arranged by size:
4791 1071 85
BsmI GAATG_Cn'
Cuts at: 3044 3159 3458 3551 3044
Size: 115 299 93 5440
Fragments arranged by size:
5440 299 115 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 3266 4008 4093 5164 1391
Size: 399 478 998 742 85 1071 2174
Fragments arranged by size:
2174 1071 998 742 478 399 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2021 2221 4442 4616 65
Size: 1183 773 200 2221 174 1396
Fragments arranged by size:
2221 1396 1183 773 200 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 3048 4751 3048
Size: 1703 4244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2374
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3175 3277 3644 4029 4046 5498 5584 5596
Size: 1203 102 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1203 385 367 346 102 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2564
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 2052 3171 513
Size: 552 987 1119 3289
Fragments arranged by size:
3289 1119 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2466 3251 2466
Size: 785 5162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 3394 4283
Size: 54 1425 50 275 18 652 912 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 912 889 652 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 3877 1353
Size: 738 1786 3423
Fragments arranged by size:
3423 1786 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4010 4607 5394 5662 1239
Size: 393 2378 597 787 268 1524
Fragments arranged by size:
2378 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2097 3038 2097
Size: 941 5006
EcoO109I rG'GnC_Cy
Cuts at: 2102 2806 2917 2102
Size: 704 111 5132
Fragments arranged by size:
5132 704 111
EcoRI G'AATT_C
Cuts at: 2277 3256 3634 2277
Size: 979 378 4590
Fragments arranged by size:
4590 979 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2100 2132 2948
Size: 32 135 32 476 32 20 32 816
Cuts at: 2948 2980 4588 4620 4662 4694 1373
Size: 32 1608 32 42 32 2626
Fragments arranged by size:
2626 1608 816 476 135 42 32 32
32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2663 2691 3112 748
Size: 1915 28 421 3583
Fragments arranged by size:
3583 1915 421 28
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2220 5061 5245 1305
Size: 813 102 2841 184 2007
Fragments arranged by size:
2841 2007 813 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2484 10
Size: 54 1425 343 652 3473
Fragments arranged by size:
3473 1425 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3905 4264 4423 518
Size: 648 1251 1488 359 159 2042
Fragments arranged by size:
2042 1488 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 3064 1052
Size: 2012 3935
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 3305 4850 1132
Size: 97 2076 1545 2229
Fragments arranged by size:
2229 2076 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3034 1348
Size: 1686 4261
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2125 2176 1108
Size: 902 115 51 4879
Fragments arranged by size:
4879 902 115 51
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2806 2917 2102
Size: 704 111 5132
Fragments arranged by size:
5132 704 111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 2642 4195 5907 2642
Size: 1553 1712 2682
Fragments arranged by size:
2682 1712 1553
SfcI C'TryA_G
Cuts at: 826 1045 2282 2346 2787 3295 4430 5111 5302
Size: 219 1237 64 441 508 1135 681 191
Cuts at: 5302 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 681 508 441 219 191
64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 3386 513
Size: 552 2321 3074
Fragments arranged by size:
3074 2321 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2541 2670 3974 3991 4144 4329 4774
Size: 1255 961 129 1304 17 153 185 445
Cuts at: 4774 325
Size: 1498
Fragments arranged by size:
1498 1304 1255 961 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 4193 5905
Size: 274 80 33 51 1443 663 1553 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1712 1553 1443 663 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI