Restriction map for
pFN21AB4367
(Circular) (Six-base) MAPSORT of: pFN21AB4367.seq Check: 3857 from: 1 to: 8077
pFN21AB4367 8077 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5887 278
Size: 53 83 186 936 4351 2468
Fragments arranged by size:
4351 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5420 1937
Size: 3483 4594
Acc65I G'GTAC_C
Cuts at: 5398 5398
Size: 8077
AclI AA'CG_TT
Cuts at: 1563 2061 6204 6577 1563
Size: 498 4143 373 3063
Fragments arranged by size:
4143 3063 498 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 2454 3072 5292 5376 6140 7524 7792
Size: 681 141 618 2220 84 764 1384 268
Cuts at: 7792 1632
Size: 1917
Fragments arranged by size:
2220 1917 1384 764 681 618 268 141
84
AfeI AGC'GCT
Cuts at: 4488 4907 4488
Size: 419 7658
AflII C'TTAA_G
Cuts at: 820 1017 2779 4480 820
Size: 197 1762 1701 4417
Fragments arranged by size:
4417 1762 1701 197
AflIII A'CryG_T
Cuts at: 1203 2692 6976 7953 1203
Size: 1489 4284 977 1327
Fragments arranged by size:
4284 1489 1327 977
AgeI A'CCGG_T
Cuts at: 6908 6908
Size: 8077
AhdI GACnn_n'nnGTC
Cuts at: 6806 6806
Size: 8077
AleI CACnn'nnGTG
Cuts at: 1166 4445 5037 1166
Size: 3279 592 4206
Fragments arranged by size:
4206 3279 592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5055 1184
Size: 3871 4206
AlwNI CAG_nnn'CTG
Cuts at: 3701 3734 4427 5012 5820 7392 3701
Size: 33 693 585 808 1572 4386
Fragments arranged by size:
4386 1572 808 693 585 33
ApaLI G'TGCA_C
Cuts at: 4179 6134 7290 4179
Size: 1955 1156 4966
Fragments arranged by size:
4966 1955 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2720 2784 3065 3475 5386 5612 5764
Size: 599 628 64 281 410 1911 226 152
Cuts at: 5764 7873 1493
Size: 2109 1697
Fragments arranged by size:
2109 1911 1697 628 599 410 281 226
152 64
AseI AT'TA_AT
Cuts at: 160 1032 3627 6631 160
Size: 872 2595 3004 1606
Fragments arranged by size:
3004 2595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
AvaI C'yCGr_G
Cuts at: 1870 1943 5402 1870
Size: 73 3459 4545
Fragments arranged by size:
4545 3459 73
BamHI G'GATC_C
Cuts at: 5407 5774 5407
Size: 367 7710
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2428 5039 5398 6853 618
Size: 325 217 627 641 2611 359 1455 1842
Fragments arranged by size:
2611 1842 1455 641 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 3837 5396 1391
Size: 2446 1559 4072
Fragments arranged by size:
4072 2446 1559
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 3380 3590 3918 4703 5112 928
Size: 971 219 1262 210 328 785 409 3893
Fragments arranged by size:
3893 1262 971 785 409 328 219 210
BbvCI CC'TCA_GC
Cuts at: 4173 4173
Size: 8077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2330 2364 6268 6302 1903
Size: 34 393 34 3904 34 3678
Fragments arranged by size:
3904 3678 393 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5969 7179 5969
Size: 1210 6867
BclI T'GATC_A
Cuts at: 1548 3400 3982 1548
Size: 1852 582 5643
Fragments arranged by size:
5643 1852 582
BfrBI ATG'CAT
Cuts at: 2498 2498
Size: 8077
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6688 136
Size: 107 122 71 631 605 5016 1525
Fragments arranged by size:
5016 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3535 8072 3535
Size: 4537 3540
BlpI GC'TnA_GC
Cuts at: 5494 5494
Size: 8077
Bme1580I G_kGCm'C
Cuts at: 1790 2456 4183 6138 7294 1790
Size: 666 1727 1955 1156 2573
Fragments arranged by size:
2573 1955 1727 1156 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4058 4426 5808
Size: 416 53 83 399 24 2641 368 1382
Cuts at: 5808 6766 442
Size: 958 1753
Fragments arranged by size:
2641 1753 1382 958 416 399 368 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4493 1056
Size: 3437 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3756 4890 6737 1239
Size: 2517 1134 1847 2579
Fragments arranged by size:
2579 2517 1847 1134
Bpu10I CC'TnA_GC
Cuts at: 2298 4173 5317 2298
Size: 1875 1144 5058
Fragments arranged by size:
5058 1875 1144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 3461 6202 7067 7365 2906
Size: 555 2741 865 298 3618
Fragments arranged by size:
3618 2741 865 555 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6740 882
Size: 617 5241 2219
Fragments arranged by size:
5241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5035 5868 493
Size: 711 3831 833 2702
Fragments arranged by size:
3831 2702 833 711
BsaBI GATnn'nnATC
Cuts at: 8071 8071
Size: 8077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5048 5884 6266 6901
Size: 53 83 186 936 3515 836 382 635
Cuts at: 6901 275
Size: 1451
Fragments arranged by size:
3515 1451 936 836 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6510 6908 7182 7329 7740 6510
Size: 398 274 147 411 6847
Fragments arranged by size:
6847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2206 2236 3145 3175 3661
Size: 30 165 30 803 30 909 30 486
Cuts at: 3661 3691 1178
Size: 30 5564
Fragments arranged by size:
5564 909 803 486 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 3153 3669 5141 5144 5213 5216 5219
Size: 1044 923 516 1472 3 69 3 3
Cuts at: 5219 5222 1186
Size: 3 4041
Fragments arranged by size:
4041 1472 1044 923 516 69 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2250 7280 7776 2250
Size: 5030 496 2551
Fragments arranged by size:
5030 2551 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2638 3846 3941 1163
Size: 573 902 1208 95 5299
Fragments arranged by size:
5299 1208 902 573 95
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5527 6288 7316 1540
Size: 275 186 3526 761 1028 2301
Fragments arranged by size:
3526 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2778 3837 4183 5034 6138 6223 7294 2778
Size: 1059 346 851 1104 85 1071 3561
Fragments arranged by size:
3561 1104 1071 1059 851 346 85
BsmI GAATG_Cn'
Cuts at: 2242 2342 2816 3817 5588 5681 2242
Size: 100 474 1001 1771 93 4638
Fragments arranged by size:
4638 1771 1001 474 100 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2952 5055 2952
Size: 2103 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 2778 3837 4183 5034 5396 6138
Size: 399 666 322 1059 346 851 362 742
Cuts at: 6138 6223 7294 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1059 851 742 666 399 362
346 322 85
BspEI T'CCGG_A
Cuts at: 7740 7740
Size: 8077
BspHI T'CATG_A
Cuts at: 2473 2581 4798 5966 7696 2473
Size: 108 2217 1168 1730 2854
Fragments arranged by size:
2854 2217 1730 1168 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 5432 844
Size: 1349 3239 3489
Fragments arranged by size:
3489 3239 1349
BsrBI CCG'CTC
Cuts at: 1394 1823 5964 1394
Size: 429 4141 3507
Fragments arranged by size:
4141 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4452 6572 6746 65
Size: 1183 3204 2120 174 1396
Fragments arranged by size:
3204 2120 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6721 6908 1700
Size: 110 4911 187 2869
Fragments arranged by size:
4911 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 4164 96
Size: 2222 1846 4009
Fragments arranged by size:
4009 2222 1846
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8077
BssSI C'ACGA_G
Cuts at: 6137 7149 6137
Size: 1012 7065
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3803 794
Size: 3009 5068
BstBI TT'CG_AA
Cuts at: 6881 6881
Size: 8077
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8077
BstXI CCAn_nnnn'nTGG
Cuts at: 4447 4595 4447
Size: 148 7929
BstYI r'GATC_y
Cuts at: 1972 3535 5407 5774 6159 6176 7628 7714 7726
Size: 1563 1872 367 385 17 1452 86 12
Cuts at: 7726 8072 1972
Size: 346 1977
Fragments arranged by size:
1977 1872 1563 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 3165 2424
Size: 741 7336
BtgI C'CryG_G
Cuts at: 513 1065 2004 2048 513
Size: 552 939 44 6542
Fragments arranged by size:
6542 939 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4931 487
Size: 683 56 3705 3633
Fragments arranged by size:
3705 3633 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 2406 4425 5593 6387 6407 2227
Size: 179 2019 1168 794 20 3897
Fragments arranged by size:
3897 2019 1168 794 179 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8042
DraI TTT'AAA
Cuts at: 3480 3771 4828 5381 3480
Size: 291 1057 553 6176
Fragments arranged by size:
6176 1057 553 291
DraIII CAC_nnn'GTG
Cuts at: 2736 4391 2736
Size: 1655 6422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2411 7084 809
Size: 507 1095 4673 1802
Fragments arranged by size:
4673 1802 1095 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3361 4449 5524
Size: 54 1425 50 275 18 1531 1088 1075
Cuts at: 5524 6413 8042 8
Size: 889 1629 43
Fragments arranged by size:
1629 1531 1425 1088 1075 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5524 1537
Size: 275 3712 4090
Fragments arranged by size:
4090 3712 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2430 2674 3825 4182 4995 5091 5118
Size: 1026 51 244 1151 357 813 96 27
Cuts at: 5118 5190 5223 5232 6007 1353
Size: 72 33 9 775 3423
Fragments arranged by size:
3423 1151 1026 813 775 357 244 96
72 51 33 27 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6664 7038 7184 387
Size: 1064 138 435 4640 374 146 1280
Fragments arranged by size:
4640 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3835 3835
Size: 8077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2454 3072 3756 4890 5292 5376
Size: 393 681 141 618 684 1134 402 84
Cuts at: 5376 6140 6737 7524 7792 1239
Size: 764 597 787 268 1524
Fragments arranged by size:
1524 1134 787 764 684 681 618 597
402 393 268 141 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3948 3948
Size: 8077
EcoO109I rG'GnC_Cy
Cuts at: 2288 5009 2288
Size: 2721 5356
EcoRI G'AATT_C
Cuts at: 2784 3065 5386 5764 2784
Size: 281 2321 378 5097
Fragments arranged by size:
5097 2321 378 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 3041 3326 3796 2848
Size: 193 285 470 7129
Fragments arranged by size:
7129 470 285 193
FspI TGC'GCA
Cuts at: 4321 6583 4321
Size: 2262 5815
HaeII r_GCGC'y
Cuts at: 1678 4490 4909 7224 1678
Size: 2812 419 2315 2531
Fragments arranged by size:
2812 2531 2315 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4610 4642 5336 5368 6718
Size: 32 135 32 3038 32 694 32 1350
Cuts at: 6718 6750 6792 6824 1373
Size: 32 42 32 2626
Fragments arranged by size:
3038 2626 1350 694 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3034 4254 5421 5667 669
Size: 988 281 1096 1220 1167 246 3079
Fragments arranged by size:
3079 1220 1167 1096 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2745 3565 748
Size: 1997 820 5260
Fragments arranged by size:
5260 1997 820
HpaI GTT'AAC
Cuts at: 3034 5667 3034
Size: 2633 5444
KpnI G_GTAC'C
Cuts at: 5402 5402
Size: 8077
MfeI C'AATT_G
Cuts at: 5676 5676
Size: 8077
MluI A'CGCG_T
Cuts at: 7953 7953
Size: 8077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3270 7191 7375 1305
Size: 1965 3921 184 2007
Fragments arranged by size:
3921 2007 1965 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3363 4451 10
Size: 54 1425 343 1531 1088 3636
Fragments arranged by size:
3636 1531 1425 1088 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2809 2932 4445 4773 4803 5037 6035
Size: 648 1643 123 1513 328 30 234 998
Cuts at: 6035 6394 6553 518
Size: 359 159 2042
Fragments arranged by size:
2042 1643 1513 998 648 359 328 234
159 123 30
MspA1I CmG'CkG
Cuts at: 1606 2050 2156 4172 5493 6170 7318 7563 1606
Size: 444 106 2016 1321 677 1148 245 2120
Fragments arranged by size:
2120 2016 1321 1148 677 444 245 106
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6586
Fragments arranged by size:
6586 939 552
NdeI CA'TA_TG
Cuts at: 387 2806 387
Size: 2419 5658
NheI G'CTAG_C
Cuts at: 1052 4489 1052
Size: 3437 4640
NotI GC'GGCC_GC
Cuts at: 5524 5524
Size: 8077
NsiI A_TGCA'T
Cuts at: 2500 2500
Size: 8077
NspI r_CATG'y
Cuts at: 1132 1229 2696 4858 5435 6980 1132
Size: 97 1467 2162 577 1545 2229
Fragments arranged by size:
2229 2162 1545 1467 577 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8077
PciI A'CATG_T
Cuts at: 2692 6976 2692
Size: 4284 3793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3200 1108
Size: 2092 5985
PfoI T'CCnGG_A
Cuts at: 3616 4293 3616
Size: 677 7400
PmeI GTTT'AAAC
Cuts at: 5381 5381
Size: 8077
PmlI CAC'GTG
Cuts at: 5035 5035
Size: 8077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6146 6178 7659 7691 6146
Size: 32 1481 32 6532
Fragments arranged by size:
6532 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8077
PsiI TTA'TAA
Cuts at: 2073 5647 2073
Size: 3574 4503
PstI C_TGCA'G
Cuts at: 830 3694 4561 5429 830
Size: 2864 867 868 3478
Fragments arranged by size:
3478 2864 868 867
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8077
PvuII CAG'CTG
Cuts at: 2156 4172 2156
Size: 2016 6061
SacI G_AGCT'C
Cuts at: 3837 3837
Size: 8077
SacII CC_GC'GG
Cuts at: 2051 2051
Size: 8077
SalI G'TCGA_C
Cuts at: 1936 5419 1936
Size: 3483 4594
SapI GCTCTTCn'nnn_
Cuts at: 3825 4182 3825
Size: 357 7720
SbfI CC_TGCA'GG
Cuts at: 5429 5429
Size: 8077
ScaI AGT'ACT
Cuts at: 6325 8037 6325
Size: 1712 6365
SfcI C'TryA_G
Cuts at: 826 1045 2851 3433 3690 4419 4557 5425 6560
Size: 219 1806 582 257 729 138 868 1135
Cuts at: 6560 7241 7432 826
Size: 681 191 1471
Fragments arranged by size:
1806 1471 1135 868 729 681 582 257
219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8077
SmaI CCC'GGG
Cuts at: 1872 5404 1872
Size: 3532 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2779 2921 3440 4480 6181 7082
Size: 197 926 836 142 519 1040 1701 901
Cuts at: 7082 7344 820
Size: 262 1553
Fragments arranged by size:
1701 1553 1040 926 901 836 519 262
197 142
SnaBI TAC'GTA
Cuts at: 493 5868 493
Size: 5375 2702
SpeI A'CTAG_T
Cuts at: 152 4262 152
Size: 4110 3967
SphI G_CATG'C
Cuts at: 1132 5435 1132
Size: 4303 3774
SspI AAT'ATT
Cuts at: 5 52 6001 5
Size: 47 5949 2081
Fragments arranged by size:
5949 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8077
StyI C'CwwG_G
Cuts at: 513 1065 2004 5516 513
Size: 552 939 3512 3074
Fragments arranged by size:
3512 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2697 3361 5027 6104 6121 6274 6459
Size: 1255 1117 664 1666 1077 17 153 185
Cuts at: 6459 6904 325
Size: 445 1498
Fragments arranged by size:
1666 1498 1255 1117 1077 664 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 3247 4106
Size: 274 80 33 51 1443 341 929 859
Cuts at: 4106 4164 6323 8035 96
Size: 58 2159 1712 138
Fragments arranged by size:
2159 1712 1443 929 859 341 274 138
80 58 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 8037 8069 2206
Size: 32 5799 32 2214
Fragments arranged by size:
5799 2214 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2701 1316
Size: 1385 6692
XbaI T'CTAG_A
Cuts at: 5413 5413
Size: 8077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8077
XmaI C'CCGG_G
Cuts at: 1870 5402 1870
Size: 3532 4545
XmnI GAAnn'nnTTC
Cuts at: 2388 6206 2388
Size: 3818 4259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5885 276
Size: 53 83 186 936 4351 2468
Fragments arranged by size:
4351 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI