Restriction map for
pFN21AB4366
(Circular) (Six-base) MAPSORT of: pFN21AB4366.seq Check: 4633 from: 1 to: 7474
pFN21AB4366 7474 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3664 3786 3664
Size: 122 7352
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5284 278
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4817 1937
Size: 2880 4594
Acc65I G'GTAC_C
Cuts at: 3890 4795 3890
Size: 905 6569
AclI AA'CG_TT
Cuts at: 1563 5601 5974 1563
Size: 4038 373 3063
Fragments arranged by size:
4038 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 4581 5537 6921 7189 1632
Size: 558 2391 956 1384 268 1917
Fragments arranged by size:
2391 1917 1384 956 558 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7277
AflIII A'CryG_T
Cuts at: 1203 3656 6373 7350 1203
Size: 2453 2717 977 1327
Fragments arranged by size:
2717 2453 1327 977
AgeI A'CCGG_T
Cuts at: 6305 6305
Size: 7474
AhdI GACnn_n'nnGTC
Cuts at: 6203 6203
Size: 7474
AleI CACnn'nnGTG
Cuts at: 1166 3770 1166
Size: 2604 4870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7474
AlwNI CAG_nnn'CTG
Cuts at: 5217 6789 5217
Size: 1572 5902
ApaLI G'TGCA_C
Cuts at: 3979 5531 6687 3979
Size: 1552 1156 4766
Fragments arranged by size:
4766 1552 1156
ApoI r'AATT_y
Cuts at: 1493 2389 3174 3200 4783 5009 5161 7270 1493
Size: 896 785 26 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 896 785 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 2441 6028 160
Size: 872 1409 3587 1606
Fragments arranged by size:
3587 1606 1409 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 4048 4799 1870
Size: 73 283 1822 751 4545
Fragments arranged by size:
4545 1822 751 283 73
BamHI G'GATC_C
Cuts at: 4804 5171 4804
Size: 367 7107
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3890 4795 6250 618
Size: 325 217 627 2103 905 1455 1842
Fragments arranged by size:
2103 1842 1455 905 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3297 4793 1391
Size: 1906 1496 4072
Fragments arranged by size:
4072 1906 1496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4641 928
Size: 971 2742 3761
Fragments arranged by size:
3761 2742 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5665 5699 1903
Size: 34 3728 34 3678
Fragments arranged by size:
3728 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4293 4456 5366 6576 4293
Size: 163 910 1210 5191
Fragments arranged by size:
5191 1210 910 163
BclI T'GATC_A
Cuts at: 1548 3478 1548
Size: 1930 5544
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6085 136
Size: 107 122 71 631 605 4413 1525
Fragments arranged by size:
4413 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3826 7469 3826
Size: 3643 3831
BlpI GC'TnA_GC
Cuts at: 4891 4891
Size: 7474
Bme1580I G_kGCm'C
Cuts at: 1790 3983 4327 5535 6691 1790
Size: 2193 344 1208 1156 2573
Fragments arranged by size:
2573 2193 1208 1156 344
BmgBI CAC'GTC
Cuts at: 1318 2857 1318
Size: 1539 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4081 4302 5205
Size: 416 53 83 399 24 2664 221 903
Cuts at: 5205 6163 442
Size: 958 1753
Fragments arranged by size:
2664 1753 958 903 416 399 221 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 3163 3267 3426 4619 4763 6134 1239
Size: 1394 530 104 159 1193 144 1371 2579
Fragments arranged by size:
2579 1394 1371 1193 530 159 144 104
Bpu10I CC'TnA_GC
Cuts at: 3663 3663
Size: 7474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2188 5599 6464 6762 2105
Size: 83 3411 865 298 2817
Fragments arranged by size:
3411 2817 865 298 83
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4665 6137 882
Size: 617 3166 1472 2219
Fragments arranged by size:
3166 2219 1472 617
BsaAI yAC'GTr
Cuts at: 493 1204 5265 493
Size: 711 4061 2702
Fragments arranged by size:
4061 2702 711
BsaBI GATnn'nnATC
Cuts at: 3444 3450 3753 7468 3444
Size: 6 303 3715 3450
Fragments arranged by size:
3715 3450 303 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2155 5281 5663 6298
Size: 53 83 186 936 622 3126 382 635
Cuts at: 6298 275
Size: 1451
Fragments arranged by size:
3126 1451 936 635 622 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5907 6305 6579 6726 7137 5907
Size: 398 274 147 411 6244
Fragments arranged by size:
6244 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4516 4546 1178
Size: 30 165 30 3113 30 4106
Fragments arranged by size:
4106 3113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2738 3423 4661 4664 1186
Size: 1552 685 1238 3 3996
Fragments arranged by size:
3996 1552 1238 685 3
BseYI C'CCAG_C
Cuts at: 6677 7173 6677
Size: 496 6978
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3227 3765 1163
Size: 573 1491 538 4872
Fragments arranged by size:
4872 1491 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2530 2673 4112 4924 5685 6713
Size: 275 186 529 143 1439 812 761 1028
Cuts at: 6713 1540
Size: 2301
Fragments arranged by size:
2301 1439 1028 812 761 529 275 186
143
BsiHKAI G_wGCw'C
Cuts at: 3664 3983 5535 5620 6691 3664
Size: 319 1552 85 1071 4447
Fragments arranged by size:
4447 1552 1071 319 85
BsmI GAATG_Cn'
Cuts at: 3218 4985 5078 3218
Size: 1767 93 5614
Fragments arranged by size:
5614 1767 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4492 4492
Size: 7474
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3297 3664 3983 4327 4793 5535 5620
Size: 399 1507 367 319 344 466 742 85
Cuts at: 5620 6691 1391
Size: 1071 2174
Fragments arranged by size:
2174 1507 1071 742 466 399 367 344
319 85
BspEI T'CCGG_A
Cuts at: 7137 7137
Size: 7474
BspHI T'CATG_A
Cuts at: 3352 3442 3913 5363 7093 3352
Size: 90 471 1450 1730 3733
Fragments arranged by size:
3733 1730 1450 471 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3664 3786 4829 844
Size: 2820 122 1043 3489
Fragments arranged by size:
3489 2820 1043 122
BsrBI CCG'CTC
Cuts at: 1394 1823 3147 5361 1394
Size: 429 1324 2214 3507
Fragments arranged by size:
3507 2214 1324 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 5969 6143 65
Size: 1183 1443 3278 174 1396
Fragments arranged by size:
3278 1443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 6118 6305 1700
Size: 110 208 4100 187 2869
Fragments arranged by size:
4100 2869 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7474
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7474
BssSI C'ACGA_G
Cuts at: 2091 5534 6546 2091
Size: 3443 1012 3019
Fragments arranged by size:
3443 3019 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7474
BstBI TT'CG_AA
Cuts at: 6278 6278
Size: 7474
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7474
BstXI CCAn_nnnn'nTGG
Cuts at: 4597 4741 4597
Size: 144 7330
BstYI r'GATC_y
Cuts at: 1972 3826 3908 4187 4804 5171 5556 5573 7025
Size: 1854 82 279 617 367 385 17 1452
Cuts at: 7025 7111 7123 7469 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1854 1452 617 385 367 346 279
86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5983
Fragments arranged by size:
5983 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6735
Fragments arranged by size:
6735 683 56
BtsI GCAGTG_nn'
Cuts at: 2888 3215 3367 4990 5784 5804 2888
Size: 327 152 1623 794 20 4558
Fragments arranged by size:
4558 1623 794 327 152 20
ClaI AT'CG_AT
Cuts at: 1554 4108 1554
Size: 2554 4920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2245 2280 529
Size: 35 1681 35 5723
Fragments arranged by size:
5723 1681 35 35
DraI TTT'AAA
Cuts at: 4778 4778
Size: 7474
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6481 809
Size: 507 5165 1802
Fragments arranged by size:
5165 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2531 3774 4178
Size: 54 1425 50 275 18 701 1243 404
Cuts at: 4178 4921 5810 7439 8
Size: 743 889 1629 43
Fragments arranged by size:
1629 1425 1243 889 743 701 404 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4921 1537
Size: 275 3109 4090
Fragments arranged by size:
4090 3109 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4495 4557 4653 4689 5404 1353
Size: 3142 62 96 36 715 3423
Fragments arranged by size:
3423 3142 715 96 62 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6061 6435 6581 387
Size: 1064 138 4472 374 146 1280
Fragments arranged by size:
4472 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2633 3163 3267 3426 4581 4619
Size: 393 558 443 530 104 159 1155 38
Cuts at: 4619 4763 5537 6134 6921 7189 1239
Size: 144 774 597 787 268 1524
Fragments arranged by size:
1524 1155 787 774 597 558 530 443
393 268 159 144 104 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3306 4742 3306
Size: 1436 6038
EcoO109I rG'GnC_Cy
Cuts at: 3311 3311
Size: 7474
EcoRI G'AATT_C
Cuts at: 4783 5161 4783
Size: 378 7096
EcoRV GAT'ATC
Cuts at: 4204 4380 4204
Size: 176 7298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2339 4754 2339
Size: 2415 5059
FspI TGC'GCA
Cuts at: 5980 5980
Size: 7474
HaeII r_GCGC'y
Cuts at: 1678 6621 1678
Size: 4943 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 3646 3678 6115
Size: 32 135 32 488 32 1554 32 2437
Cuts at: 6115 6147 6189 6221 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2437 1554 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 4818 5064 669
Size: 988 281 625 2255 246 3079
Fragments arranged by size:
3079 2255 988 625 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7474
HpaI GTT'AAC
Cuts at: 5064 5064
Size: 7474
KpnI G_GTAC'C
Cuts at: 3894 4799 3894
Size: 905 6569
MfeI C'AATT_G
Cuts at: 2304 5073 2304
Size: 2769 4705
MluI A'CGCG_T
Cuts at: 7350 7350
Size: 7474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2693 6588 6772 1305
Size: 1388 3895 184 2007
Fragments arranged by size:
3895 2007 1388 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3776 4180 10
Size: 54 1425 343 1944 404 3304
Fragments arranged by size:
3304 1944 1425 404 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 2478 2970 3606 3759 3770 5432
Size: 648 1126 186 492 636 153 11 1662
Cuts at: 5432 5791 5950 518
Size: 359 159 2042
Fragments arranged by size:
2042 1662 1126 648 636 492 359 186
159 153 11
MspA1I CmG'CkG
Cuts at: 1606 2363 4559 4668 4890 5567 6715 6960 1606
Size: 757 2196 109 222 677 1148 245 2120
Fragments arranged by size:
2196 2120 1148 757 677 245 222 109
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 7474
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5983
Fragments arranged by size:
5983 939 552
NdeI CA'TA_TG
Cuts at: 387 2623 2997 387
Size: 2236 374 4864
Fragments arranged by size:
4864 2236 374
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 7474
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7474
NotI GC'GGCC_GC
Cuts at: 4921 4921
Size: 7474
NspI r_CATG'y
Cuts at: 1132 1229 3114 3660 4832 6377 1132
Size: 97 1885 546 1172 1545 2229
Fragments arranged by size:
2229 1885 1545 1172 546 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7474
PciI A'CATG_T
Cuts at: 3656 6373 3656
Size: 2717 4757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7474
PmeI GTTT'AAAC
Cuts at: 4778 4778
Size: 7474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5543 5575 7056 7088 5543
Size: 32 1481 32 5929
Fragments arranged by size:
5929 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3311 3311
Size: 7474
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7474
PsiI TTA'TAA
Cuts at: 5044 5044
Size: 7474
PspXI vC'TCGA_Gb
Cuts at: 2226 2226
Size: 7474
PstI C_TGCA'G
Cuts at: 830 2467 2895 4826 830
Size: 1637 428 1931 3478
Fragments arranged by size:
3478 1931 1637 428
PvuI CG_AT'CG
Cuts at: 2001 4112 2001
Size: 2111 5363
PvuII CAG'CTG
Cuts at: 2363 4559 2363
Size: 2196 5278
SalI G'TCGA_C
Cuts at: 1936 4816 1936
Size: 2880 4594
SapI GCTCTTCn'nnn_
Cuts at: 4689 4689
Size: 7474
SbfI CC_TGCA'GG
Cuts at: 4826 4826
Size: 7474
ScaI AGT'ACT
Cuts at: 2053 2709 5722 7434 2053
Size: 656 3013 1712 2093
Fragments arranged by size:
3013 2093 1712 656
SfcI C'TryA_G
Cuts at: 826 1045 2463 2644 2891 3288 3501 3903 4455
Size: 219 1418 181 247 397 213 402 552
Cuts at: 4455 4822 5957 6638 6829 826
Size: 367 1135 681 191 1471
Fragments arranged by size:
1471 1418 1135 681 552 402 397 367
247 219 213 191 181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7474
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7474
SmaI CCC'GGG
Cuts at: 1872 4801 1872
Size: 2929 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2203 2226 5578 6479 6741
Size: 197 926 141 119 23 3352 901 262
Cuts at: 6741 820
Size: 1553
Fragments arranged by size:
3352 1553 926 901 262 197 141 119
23
SnaBI TAC'GTA
Cuts at: 493 5265 493
Size: 4772 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7474
SphI G_CATG'C
Cuts at: 1132 4832 1132
Size: 3700 3774
SspI AAT'ATT
Cuts at: 5 52 4065 5398 5
Size: 47 4013 1333 2081
Fragments arranged by size:
4013 2081 1333 47
StuI AGG'CCT
Cuts at: 1517 2842 1517
Size: 1325 6149
StyI C'CwwG_G
Cuts at: 513 1065 2004 4675 4913 513
Size: 552 939 2671 238 3074
Fragments arranged by size:
3074 2671 939 552 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 3099 5501 5518 5671 5856 6301
Size: 1255 869 650 2402 17 153 185 445
Cuts at: 6301 325
Size: 1498
Fragments arranged by size:
2402 1498 1255 869 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2051 2707 3592
Size: 274 80 33 51 1443 74 656 885
Cuts at: 3592 4068 4317 5720 7432 96
Size: 476 249 1403 1712 138
Fragments arranged by size:
1712 1443 1403 885 656 476 274 249
138 80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4516 4548 7434 7466 4516
Size: 32 2886 32 4524
Fragments arranged by size:
4524 2886 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7474
XbaI T'CTAG_A
Cuts at: 4810 4810
Size: 7474
XhoI C'TCGA_G
Cuts at: 1943 2226 1943
Size: 283 7191
XmaI C'CCGG_G
Cuts at: 1870 4799 1870
Size: 2929 4545
XmnI GAAnn'nnTTC
Cuts at: 5603 5603
Size: 7474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5282 276
Size: 53 83 186 936 3748 2468
Fragments arranged by size:
3748 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI