(Circular) (Six-base) MAPSORT of: pFN21AB4360.seq Check: 5169 from: 1 to: 7090
pFN21AB4360 7090 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3501 3501
Size: 7090
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3333 4900 278
Size: 53 83 186 936 1797 1567 2468
Fragments arranged by size:
2468 1797 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 4065 4433 1937
Size: 1113 1015 368 4594
Fragments arranged by size:
4594 1113 1015 368
Acc65I G'GTAC_C
Cuts at: 4411 4411
Size: 7090
AclI AA'CG_TT
Cuts at: 1563 5217 5590 1563
Size: 3654 373 3063
Fragments arranged by size:
3654 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4322 5153 6537 6805 1632
Size: 2690 831 1384 268 1917
Fragments arranged by size:
2690 1917 1384 831 268
AfeI AGC'GCT
Cuts at: 2601 2601
Size: 7090
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6893
AflIII A'CryG_T
Cuts at: 1203 5989 6966 1203
Size: 4786 977 1327
Fragments arranged by size:
4786 1327 977
AgeI A'CCGG_T
Cuts at: 5921 5921
Size: 7090
AhdI GACnn_n'nnGTC
Cuts at: 5819 5819
Size: 7090
AleI CACnn'nnGTG
Cuts at: 1166 3209 1166
Size: 2043 5047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7090
AlwNI CAG_nnn'CTG
Cuts at: 2453 4833 6405 2453
Size: 2380 1572 3138
Fragments arranged by size:
3138 2380 1572
ApaI G_GGCC'C
Cuts at: 4193 4193
Size: 7090
ApaLI G'TGCA_C
Cuts at: 5147 6303 5147
Size: 1156 5934
ApoI r'AATT_y
Cuts at: 1493 2527 2726 2901 4399 4625 4777 6886 1493
Size: 1034 199 175 1498 226 152 2109 1697
Fragments arranged by size:
2109 1697 1498 1034 226 199 175 152
AseI AT'TA_AT
Cuts at: 160 1032 5644 160
Size: 872 4612 1606
Fragments arranged by size:
4612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2759 2802 3732 3787 3836 4184
Size: 73 244 572 43 930 55 49 348
Cuts at: 4184 4415 1870
Size: 231 4545
Fragments arranged by size:
4545 930 572 348 244 231 73 55
49 43
BamHI G'GATC_C
Cuts at: 4420 4787 4420
Size: 367 6723
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 3443 3709 3727 4180
Size: 325 217 627 968 688 266 18 453
Cuts at: 4180 4223 4411 5866 618
Size: 43 188 1455 1842
Fragments arranged by size:
1842 1455 968 688 627 453 325 266
217 188 43 18
BanII G_rGCy'C
Cuts at: 1391 2226 2628 2662 3837 4193 4409 1391
Size: 835 402 34 1175 356 216 4072
Fragments arranged by size:
4072 1175 835 402 356 216 34
BbeI G_GCGC'C
Cuts at: 3713 3713
Size: 7090
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4068 4102 5281 5315 1903
Size: 34 2131 34 1179 34 3678
Fragments arranged by size:
3678 2131 1179 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3699 4982 6192 3699
Size: 1283 1210 4597
Fragments arranged by size:
4597 1283 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7090
BfrBI ATG'CAT
Cuts at: 2203 2203
Size: 7090
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 3575 5701
Size: 107 122 71 631 605 475 1428 2126
Cuts at: 5701 136
Size: 1525
Fragments arranged by size:
2126 1525 1428 631 605 475 122 107
71
BglII A'GATC_T
Cuts at: 7085 7085
Size: 7090
BlpI GC'TnA_GC
Cuts at: 2627 3623 3890 4507 2627
Size: 996 267 617 5210
Fragments arranged by size:
5210 996 617 267
Bme1580I G_kGCm'C
Cuts at: 1790 2760 3732 4185 4193 5151 6307 1790
Size: 970 972 453 8 958 1156 2573
Fragments arranged by size:
2573 1156 972 970 958 453 8
BmgBI CAC'GTC
Cuts at: 1318 4216 1318
Size: 2898 4192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4821 5779 442
Size: 416 53 83 399 24 3404 958 1753
Fragments arranged by size:
3404 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2021 1056
Size: 965 6125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 7090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3043 5750 1239
Size: 1804 2707 2579
Fragments arranged by size:
2707 2579 1804
Bpu10I CC'TnA_GC
Cuts at: 2622 2622
Size: 7090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3806 5215 6080 6378 3806
Size: 1409 865 298 4518
Fragments arranged by size:
4518 1409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3328 3517 5753 882
Size: 617 1829 189 2236 2219
Fragments arranged by size:
2236 2219 1829 617 189
BsaAI yAC'GTr
Cuts at: 493 1204 3979 4881 493
Size: 711 2775 902 2702
Fragments arranged by size:
2775 2702 902 711
BsaBI GATnn'nnATC
Cuts at: 7084 7084
Size: 7090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3330 3660 3710 4897
Size: 53 83 186 936 1797 330 50 1187
Cuts at: 4897 5279 5914 275
Size: 382 635 1451
Fragments arranged by size:
1797 1451 1187 936 635 382 330 186
83 53 50
BsaWI w'CCGG_w
Cuts at: 4268 4368 5523 5921 6195 6342 6753 4268
Size: 100 1155 398 274 147 411 4605
Fragments arranged by size:
4605 1155 411 398 274 147 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 3596 3626 3983
Size: 30 165 30 870 30 1293 30 357
Cuts at: 3983 4013 1178
Size: 30 4255
Fragments arranged by size:
4255 1293 870 357 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 4162 1186
Size: 1441 1535 4114
Fragments arranged by size:
4114 1535 1441
BseYI C'CCAG_C
Cuts at: 2442 4002 6293 6789 2442
Size: 1560 2291 496 2743
Fragments arranged by size:
2743 2291 1560 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2628 3389 3959 4162 1163
Size: 573 630 262 761 570 203 4091
Fragments arranged by size:
4091 761 630 573 570 262 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3741 4167 4171 4540 5301 6329
Size: 275 186 1740 426 4 369 761 1028
Cuts at: 6329 1540
Size: 2301
Fragments arranged by size:
2301 1740 1028 761 426 369 275 186
4
BsiHKAI G_wGCw'C
Cuts at: 2226 2441 2628 3306 3984 4119 5151 5236 6307
Size: 215 187 678 678 135 1032 85 1071
Cuts at: 6307 2226
Size: 3009
Fragments arranged by size:
3009 1071 1032 678 678 215 187 135
85
BsmI GAATG_Cn'
Cuts at: 2205 4601 4694 2205
Size: 2396 93 4601
Fragments arranged by size:
4601 2396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2441 2628 2662 2760 3306 3732
Size: 399 436 215 187 34 98 546 426
Cuts at: 3732 3837 3984 4119 4185 4193 4409 5151 5236
Size: 105 147 135 66 8 216 742 85
Cuts at: 5236 6307 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 546 436 426 399 216
215 187 147 135 105 98 85 66
34 8
BspEI T'CCGG_A
Cuts at: 4368 6753 4368
Size: 2385 4705
BspHI T'CATG_A
Cuts at: 4979 6709 4979
Size: 1730 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3501 4020 4445 844
Size: 2657 519 425 3489
Fragments arranged by size:
3489 2657 519 425
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 2801 4221 4977 1394
Size: 429 794 184 1420 756 3507
Fragments arranged by size:
3507 1420 794 756 429 184
BsrDI GCAATG_nn'
Cuts at: 65 1248 5585 5759 65
Size: 1183 4337 174 1396
Fragments arranged by size:
4337 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2246 4283 5734 5921 1700
Size: 110 436 2037 1451 187 2869
Fragments arranged by size:
2869 2037 1451 436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7090
BssHII G'CGCG_C
Cuts at: 1925 2193 1925
Size: 268 6822
BssSI C'ACGA_G
Cuts at: 5150 6162 5150
Size: 1012 6078
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7090
BstBI TT'CG_AA
Cuts at: 5894 5894
Size: 7090
BstEII G'GTnAC_C
Cuts at: 1184 3457 3757 1184
Size: 2273 300 4517
Fragments arranged by size:
4517 2273 300
BstXI CCAn_nnnn'nTGG
Cuts at: 3769 4337 3769
Size: 568 6522
BstYI r'GATC_y
Cuts at: 1972 4420 4787 5172 5189 6641 6727 6739 7085
Size: 2448 367 385 17 1452 86 12 346
Cuts at: 7085 1972
Size: 1977
Fragments arranged by size:
2448 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3548 3548
Size: 7090
BtgI C'CryG_G
Cuts at: 513 1065 2004 3607 3828 513
Size: 552 939 1603 221 3775
Fragments arranged by size:
3775 1603 939 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2871 487
Size: 683 56 1645 4706
Fragments arranged by size:
4706 1645 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3421 4137 4606 5400 5420 2640
Size: 781 716 469 794 20 4310
Fragments arranged by size:
4310 794 781 716 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7055
DraI TTT'AAA
Cuts at: 4394 4394
Size: 7090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6097 809
Size: 507 4781 1802
Fragments arranged by size:
4781 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2181 3532
Size: 54 1425 50 275 18 309 42 1351
Cuts at: 3532 3738 3906 3960 4537 5426 7055 8
Size: 206 168 54 577 889 1629 43
Fragments arranged by size:
1629 1425 1351 889 577 309 275 206
168 54 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3738 4537 1537
Size: 275 1926 799 4090
Fragments arranged by size:
4090 1926 799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4106 5020 1353
Size: 2753 914 3423
Fragments arranged by size:
3423 2753 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2652 5677 6051 6197 387
Size: 1064 138 1063 3025 374 146 1280
Fragments arranged by size:
3025 1280 1064 1063 374 146 138
EcoICRI GAG'CTC
Cuts at: 2224 2626 2224
Size: 402 6688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3043 4322 5153 5750 6537 6805 1239
Size: 393 1411 1279 831 597 787 268 1524
Fragments arranged by size:
1524 1411 1279 831 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2977 3568 4189 4190 4230 2977
Size: 591 621 1 40 5837
Fragments arranged by size:
5837 621 591 40 1
EcoRI G'AATT_C
Cuts at: 4399 4777 4399
Size: 378 6712
FspI TGC'GCA
Cuts at: 3875 5596 3875
Size: 1721 5369
FspAI rTGC'GCAy
Cuts at: 3875 3875
Size: 7090
HaeII r_GCGC'y
Cuts at: 1678 2603 3426 3623 3638 3713 4338 6237 1678
Size: 925 823 197 15 75 625 1899 2531
Fragments arranged by size:
2531 1899 925 823 625 197 75 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3107 3139 3422 3454 3596
Size: 32 135 32 1535 32 283 32 142
Cuts at: 3596 3628 3770 3802 3983 4015 5731 5763 5805
Size: 32 142 32 181 32 1716 32 42
Cuts at: 5805 5837 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 1535 283 181 142 142 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4434 4680 669
Size: 988 281 2496 246 3079
Fragments arranged by size:
3079 2496 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7090
HpaI GTT'AAC
Cuts at: 4680 4680
Size: 7090
KasI G'GCGC_C
Cuts at: 3709 3709
Size: 7090
KpnI G_GTAC'C
Cuts at: 4415 4415
Size: 7090
MfeI C'AATT_G
Cuts at: 4689 4689
Size: 7090
MluI A'CGCG_T
Cuts at: 6966 6966
Size: 7090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3351 6204 6388 1305
Size: 2046 2853 184 2007
Fragments arranged by size:
2853 2046 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2183 10
Size: 54 1425 343 309 42 4917
Fragments arranged by size:
4917 1425 343 309 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 3209 3275 5048 5407 5566 518
Size: 648 1372 671 66 1773 359 159 2042
Fragments arranged by size:
2042 1773 1372 671 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 3609 3694 3817 4018 4506 5183 6331 6576
Size: 2003 85 123 201 488 677 1148 245
Cuts at: 6576 1606
Size: 2120
Fragments arranged by size:
2120 2003 1148 677 488 245 201 123
85
NaeI GCC'GGC
Cuts at: 4285 4285
Size: 7090
NarI GG'CG_CC
Cuts at: 3710 3710
Size: 7090
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5599
Fragments arranged by size:
5599 939 552
NdeI CA'TA_TG
Cuts at: 387 2824 387
Size: 2437 4653
NgoMIV G'CCGG_C
Cuts at: 4283 4283
Size: 7090
NheI G'CTAG_C
Cuts at: 1052 2017 1052
Size: 965 6125
NotI GC'GGCC_GC
Cuts at: 4537 4537
Size: 7090
NsiI A_TGCA'T
Cuts at: 2205 2205
Size: 7090
NspI r_CATG'y
Cuts at: 1132 1229 3853 4448 5993 1132
Size: 97 2624 595 1545 2229
Fragments arranged by size:
2624 2229 1545 595 97
PasI CC'CwG_GG
Cuts at: 1348 4330 1348
Size: 2982 4108
PciI A'CATG_T
Cuts at: 5989 5989
Size: 7090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7090
PfoI T'CCnGG_A
Cuts at: 2672 2672
Size: 7090
PmeI GTTT'AAAC
Cuts at: 4394 4394
Size: 7090
PmlI CAC'GTG
Cuts at: 3979 3979
Size: 7090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5159 5191 6672 6704 4280
Size: 32 847 32 1481 32 4666
Fragments arranged by size:
4666 1481 847 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4230 4230
Size: 7090
PshAI GACnn'nnGTC
Cuts at: 1613 2160 1613
Size: 547 6543
PsiI TTA'TAA
Cuts at: 2946 4660 2946
Size: 1714 5376
PspOMI G'GGCC_C
Cuts at: 4189 4189
Size: 7090
PstI C_TGCA'G
Cuts at: 830 2370 3576 3699 4442 830
Size: 1540 1206 123 743 3478
Fragments arranged by size:
3478 1540 1206 743 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7090
PvuII CAG'CTG
Cuts at: 3817 3817
Size: 7090
SacI G_AGCT'C
Cuts at: 2226 2628 2226
Size: 402 6688
SacII CC_GC'GG
Cuts at: 3610 3610
Size: 7090
SalI G'TCGA_C
Cuts at: 1936 4432 1936
Size: 2496 4594
SanDI GG'GwC_CC
Cuts at: 4230 4230
Size: 7090
SapI GCTCTTCn'nnn_
Cuts at: 4106 4106
Size: 7090
SbfI CC_TGCA'GG
Cuts at: 3576 4442 3576
Size: 866 6224
ScaI AGT'ACT
Cuts at: 5338 7050 5338
Size: 1712 5378
SexAI A'CCwGG_T
Cuts at: 3753 4068 3753
Size: 315 6775
SfcI C'TryA_G
Cuts at: 826 1045 2366 3003 3090 3572 3695 4438 5573
Size: 219 1321 637 87 482 123 743 1135
Cuts at: 5573 6254 6445 826
Size: 681 191 1471
Fragments arranged by size:
1471 1321 1135 743 681 637 482 219
191 123 87
SfoI GGC'GCC
Cuts at: 3711 3711
Size: 7090
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7090
SmaI CCC'GGG
Cuts at: 1872 3734 3838 4417 1872
Size: 1862 104 579 4545
Fragments arranged by size:
4545 1862 579 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2802 3821 5194 6095 6357 820
Size: 197 926 859 1019 1373 901 262 1553
Fragments arranged by size:
1553 1373 1019 926 901 859 262 197
SnaBI TAC'GTA
Cuts at: 493 4881 493
Size: 4388 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7090
SphI G_CATG'C
Cuts at: 1132 4448 1132
Size: 3316 3774
SrfI GCCC'GGGC
Cuts at: 3838 3838
Size: 7090
SspI AAT'ATT
Cuts at: 5 52 5014 5
Size: 47 4962 2081
Fragments arranged by size:
4962 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7090
StyI C'CwwG_G
Cuts at: 513 1065 2004 2851 3762 4529 513
Size: 552 939 847 911 767 3074
Fragments arranged by size:
3074 939 911 847 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 3301 3371 4157 5117 5134 5287
Size: 1255 876 845 70 786 960 17 153
Cuts at: 5287 5472 5917 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 960 876 845 786 445 185
153 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3249 5336 7048
Size: 274 80 33 51 1443 1272 2087 1712
Cuts at: 7048 96
Size: 138
Fragments arranged by size:
2087 1712 1443 1272 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 7050 7082 2273
Size: 32 4745 32 2281
Fragments arranged by size:
4745 2281 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7090
XbaI T'CTAG_A
Cuts at: 4426 4426
Size: 7090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3916 3916
Size: 7090
XhoI C'TCGA_G
Cuts at: 1943 2802 1943
Size: 859 6231
XmaI C'CCGG_G
Cuts at: 1870 3732 3836 4415 1870
Size: 1862 104 579 4545
Fragments arranged by size:
4545 1862 579 104
XmnI GAAnn'nnTTC
Cuts at: 5219 5219
Size: 7090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3331 4898 276
Size: 53 83 186 936 1797 1567 2468
Fragments arranged by size:
2468 1797 1567 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI NruI PacI PspXI
PsrI RsrII SfiI SwaI