Restriction map for
pFN21AB4343
(Circular) (Six-base) MAPSORT of: pFN21AB4343.seq Check: 3546 from: 1 to: 5797
pFN21AB4343 5797 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 3140 1937
Size: 667 536 4594
Fragments arranged by size:
4594 667 536
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2445 2712 3860 5244 5512 1632
Size: 813 267 1148 1384 268 1917
Fragments arranged by size:
1917 1384 1148 813 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2749 1166
Size: 1583 4214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 2029 3540 5112 2029
Size: 1511 1572 2714
Fragments arranged by size:
2714 1572 1511
ApaI G_GGCC'C
Cuts at: 2014 2014
Size: 5797
ApaLI G'TGCA_C
Cuts at: 2664 3004 3854 5010 2664
Size: 340 850 1156 3451
Fragments arranged by size:
3451 1156 850 340
ApoI r'AATT_y
Cuts at: 1493 2610 3060 3106 3332 3484 5593 1493
Size: 1117 450 46 226 152 2109 1697
Fragments arranged by size:
2109 1697 1117 450 226 152 46
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2014 3045 3116 1391
Size: 623 1031 71 4072
Fragments arranged by size:
4072 1031 623 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4826
BbvCI CC'TCA_GC
Cuts at: 2294 2294
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4408 136
Size: 107 122 71 631 605 337 2399 1525
Fragments arranged by size:
2399 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2668 3008 3858 5014 1790
Size: 224 654 340 850 1156 2573
Fragments arranged by size:
2573 1156 850 654 340 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 3528 4486
Size: 416 53 83 399 24 622 1489 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1489 958 622 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4457 1239
Size: 3218 2579
Bpu10I CC'TnA_GC
Cuts at: 2294 2294
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 3922 4787 5085 2186
Size: 1736 865 298 2898
Fragments arranged by size:
2898 1736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2224 2515 4460 882
Size: 617 725 291 1945 2219
Fragments arranged by size:
2219 1945 725 617 291
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 2409 5791 2409
Size: 3382 2415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 3604 3986 4621
Size: 53 83 186 936 921 1150 382 635
Cuts at: 4621 275
Size: 1451
Fragments arranged by size:
1451 1150 936 921 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2981 3011 1178
Size: 30 165 30 1578 30 3964
Fragments arranged by size:
3964 1578 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5797
BseYI C'CCAG_C
Cuts at: 5000 5496 5000
Size: 496 5301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2773 2941 3109 1163
Size: 573 1037 168 168 3851
Fragments arranged by size:
3851 1037 573 168 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2668 3008 3045 3858 3943 5014 2668
Size: 340 37 813 85 1071 3451
Fragments arranged by size:
3451 1071 813 340 85 37
BsmI GAATG_Cn'
Cuts at: 2590 3308 3401 2590
Size: 718 93 4986
Fragments arranged by size:
4986 718 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2668 3008 3045 3116 3858 3943
Size: 399 224 654 340 37 71 742 85
Cuts at: 3943 5014 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 654 399 340 224 85
71 37
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 3152 844
Size: 1417 891 3489
Fragments arranged by size:
3489 1417 891
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792
Size: 1155 367 385 17 1452 86 12 346
Cuts at: 5792 1972
Size: 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2015 513
Size: 552 950 4295
Fragments arranged by size:
4295 950 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 3012 3012
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762
Size: 54 1425 50 275 18 1414 889 1629
Cuts at: 5762 8
Size: 43
Fragments arranged by size:
1629 1425 1414 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2147 3727 1353
Size: 723 71 1580 3423
Fragments arranged by size:
3423 1580 723 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3043 3043
Size: 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2445 2712 3860 4457 5244 5512 1239
Size: 393 813 267 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 813 787 597 393 268 267
EcoNI CCTnn'n_nnAGG
Cuts at: 2325 2325
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2011 2011
Size: 5797
EcoRI G'AATT_C
Cuts at: 3060 3106 3484 3060
Size: 46 378 5373
Fragments arranged by size:
5373 378 46
EcoRV GAT'ATC
Cuts at: 2096 2096
Size: 5797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2508 2508
Size: 5797
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373
Size: 32 135 32 2866 32 42 32 2626
Fragments arranged by size:
2866 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2749 3755 4114 4273 518
Size: 648 1583 1006 359 159 2042
Fragments arranged by size:
2042 1583 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 3213 3890 5038 5283 1606
Size: 411 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 411 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2076 2633 1348
Size: 728 557 4512
Fragments arranged by size:
4512 728 557
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2639 1108
Size: 1531 4266
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2010 2010
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
SacI G_AGCT'C
Cuts at: 3045 3045
Size: 5797
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 3145 4280 4961 5152 826
Size: 219 2100 1135 681 191 1471
Fragments arranged by size:
2100 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3901 4802 5064 820
Size: 197 926 222 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 2556 3721 5
Size: 47 2504 1165 2081
Fragments arranged by size:
2504 2081 1165 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2058 3236 513
Size: 552 993 1178 3074
Fragments arranged by size:
3074 1178 993 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2370 3824 3841 3994 4179 4624 325
Size: 1255 790 1454 17 153 185 445 1498
Fragments arranged by size:
1498 1454 1255 790 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2474 2891 4043
Size: 274 80 33 51 1443 497 417 1152
Cuts at: 4043 5755 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1152 497 417 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2535 2535
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI