Restriction map for
pFN21AB4336
(Circular) (Six-base) MAPSORT of: pFN21AB4336.seq Check: 436 from: 1 to: 7984
pFN21AB4336 7984 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5794 278
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 2440 4905 5327 1937
Size: 136 367 2465 422 4594
Fragments arranged by size:
4594 2465 422 367 136
Acc65I G'GTAC_C
Cuts at: 5027 5305 5027
Size: 278 7706
AclI AA'CG_TT
Cuts at: 1563 6111 6484 1563
Size: 4548 373 3063
Fragments arranged by size:
4548 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2530 3120 3413 4193 4808 6047 7431 7699
Size: 898 590 293 780 615 1239 1384 268
Cuts at: 7699 1632
Size: 1917
Fragments arranged by size:
1917 1384 1239 898 780 615 590 293
268
AflII C'TTAA_G
Cuts at: 820 1017 2747 820
Size: 197 1730 6057
Fragments arranged by size:
6057 1730 197
AflIII A'CryG_T
Cuts at: 1203 2088 2257 6883 7860 1203
Size: 885 169 4626 977 1327
Fragments arranged by size:
4626 1327 977 885 169
AgeI A'CCGG_T
Cuts at: 6815 6815
Size: 7984
AhdI GACnn_n'nnGTC
Cuts at: 3286 5168 6713 3286
Size: 1882 1545 4557
Fragments arranged by size:
4557 1882 1545
AleI CACnn'nnGTG
Cuts at: 1166 3359 3836 1166
Size: 2193 477 5314
Fragments arranged by size:
5314 2193 477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4551 1184
Size: 3367 4617
AlwNI CAG_nnn'CTG
Cuts at: 4058 4123 4210 4225 4864 4951 5054 5132 5727
Size: 65 87 15 639 87 103 78 595
Cuts at: 5727 7299 4058
Size: 1572 4743
Fragments arranged by size:
4743 1572 639 595 103 87 87 78
65 15
ApaI G_GGCC'C
Cuts at: 3027 3219 3262 3482 3750 4338 4567 4765 3027
Size: 192 43 220 268 588 229 198 6246
Fragments arranged by size:
6246 588 268 229 220 198 192 43
ApaLI G'TGCA_C
Cuts at: 6041 7197 6041
Size: 1156 6828
ApoI r'AATT_y
Cuts at: 1493 5293 5519 5671 7780 1493
Size: 3800 226 152 2109 1697
Fragments arranged by size:
3800 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6538 160
Size: 872 5506 1606
Fragments arranged by size:
5506 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2404 3161 3261 4465 4613 5309
Size: 73 339 122 757 100 1204 148 696
Cuts at: 5309 1870
Size: 4545
Fragments arranged by size:
4545 1204 757 696 339 148 122 100
73
BamHI G'GATC_C
Cuts at: 5314 5681 5314
Size: 367 7617
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 3265 3323 3902 4609
Size: 325 217 627 347 1131 58 579 707
Cuts at: 4609 5027 5305 6760 618
Size: 418 278 1455 1842
Fragments arranged by size:
1842 1455 1131 707 627 579 418 347
325 278 217 58
BanII G_rGCy'C
Cuts at: 1391 2319 3027 3059 3168 3219 3262 3482 3750
Size: 928 708 32 109 51 43 220 268
Cuts at: 3750 4338 4394 4535 4567 4701 4765 4970 5083
Size: 588 56 141 32 134 64 205 113
Cuts at: 5083 5303 1391
Size: 220 4072
Fragments arranged by size:
4072 928 708 588 268 220 220 205
141 134 113 109 64 56 51 43
32 32
BbeI G_GCGC'C
Cuts at: 3269 3269
Size: 7984
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 2904 3193 3999 928
Size: 971 665 340 289 806 4913
Fragments arranged by size:
4913 971 806 665 340 289
BbvCI CC'TCA_GC
Cuts at: 3154 3776 4647 5198 5263 3154
Size: 622 871 551 65 5875
Fragments arranged by size:
5875 871 622 551 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6175 6209 1903
Size: 34 4238 34 3678
Fragments arranged by size:
4238 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5876 7086 5876
Size: 1210 6774
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2471 3512 4755
Size: 107 122 71 631 605 799 1041 1243
Cuts at: 4755 5087 6595 136
Size: 332 1508 1525
Fragments arranged by size:
1525 1508 1243 1041 799 631 605 332
122 107 71
BglII A'GATC_T
Cuts at: 7979 7979
Size: 7984
BlpI GC'TnA_GC
Cuts at: 5401 5401
Size: 7984
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2210 2257 2272 2467 3027 3219 3262
Size: 298 122 47 15 195 560 192 43
Cuts at: 3262 3473 3482 3508 3750 4284 4338 4567 4612
Size: 211 9 26 242 534 54 229 45
Cuts at: 4612 4765 6045 7201 1790
Size: 153 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 560 534 298 242 229
211 195 192 153 122 54 47 45
43 26 15 9
BmgBI CAC'GTC
Cuts at: 1318 2455 3298 1318
Size: 1137 843 6004
Fragments arranged by size:
6004 1137 843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 3495 5715
Size: 416 53 83 399 24 2066 12 2220
Cuts at: 5715 6673 442
Size: 958 1753
Fragments arranged by size:
2220 2066 1753 958 416 399 83 53
24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 2619 3107 3229 3570 3653 3718 3977
Size: 1283 97 488 122 341 83 65 259
Cuts at: 3977 4145 4508 4829 4838 5097 5151 6644 1239
Size: 168 363 321 9 259 54 1493 2579
Fragments arranged by size:
2579 1493 1283 488 363 341 321 259
259 168 122 97 83 65 54 9
Bpu10I CC'TnA_GC
Cuts at: 3154 3250 3776 4226 4647 5198 5263 3154
Size: 96 526 450 421 551 65 5875
Fragments arranged by size:
5875 551 526 450 421 96 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4396 4765 6109 6974 7272 3646
Size: 750 369 1344 865 298 4358
Fragments arranged by size:
4358 1344 865 750 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2830 2867 3368 6647 882
Size: 617 1331 37 501 3279 2219
Fragments arranged by size:
3279 2219 1331 617 501 37
BsaAI yAC'GTr
Cuts at: 493 1204 2260 2335 5775 493
Size: 711 1056 75 3440 2702
Fragments arranged by size:
3440 2702 1056 711 75
BsaBI GATnn'nnATC
Cuts at: 7978 7978
Size: 7984
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3266 4152 5791 6173
Size: 53 83 186 936 1733 886 1639 382
Cuts at: 6173 6808 275
Size: 635 1451
Fragments arranged by size:
1733 1639 1451 936 886 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3972 6417 6815 7089 7236 7647 3972
Size: 2445 398 274 147 411 4309
Fragments arranged by size:
4309 2445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 3092 3095 3122
Size: 30 165 30 1407 30 252 3 27
Cuts at: 3122 3125 3668 3698 3989 4019 5070 5100 1178
Size: 3 543 30 291 30 1051 30 4062
Fragments arranged by size:
4062 1407 1051 543 291 252 165 30
30 30 30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2604 2616 2619 2665 2818 2821 2977
Size: 1204 214 12 3 46 153 3 156
Cuts at: 2977 2989 2992 3001 3004 3113 3436 3721 3799
Size: 12 3 9 3 109 323 285 78
Cuts at: 3799 3908 4511 1186
Size: 109 603 4659
Fragments arranged by size:
4659 1204 603 323 285 214 156 153
109 109 78 46 12 12 9 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2052 2250 2783 3481 3507 3620 4584 4627 4961
Size: 198 533 698 26 113 964 43 334
Cuts at: 4961 4971 7187 7683 2052
Size: 10 2216 496 2353
Fragments arranged by size:
2353 2216 964 698 533 496 334 198
113 43 26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2113 4044 4166 4289 1163
Size: 573 377 1931 122 123 4858
Fragments arranged by size:
4858 1931 573 377 123 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5434 6195 7223 1540
Size: 275 186 3433 761 1028 2301
Fragments arranged by size:
3433 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 3059 4382 6045 6130 7201 2307
Size: 752 1323 1663 85 1071 3090
Fragments arranged by size:
3090 1663 1323 1071 752 85
BsmI GAATG_Cn'
Cuts at: 3074 5495 5588 3074
Size: 2421 93 5470
Fragments arranged by size:
5470 2421 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2210 2257 2272 2307 2319 2467
Size: 399 298 122 47 15 35 12 148
Cuts at: 2467 3027 3059 3168 3219 3262 3473 3482 3508
Size: 560 32 109 51 43 211 9 26
Cuts at: 3508 3750 4284 4338 4382 4394 4535 4567 4612
Size: 242 534 54 44 12 141 32 45
Cuts at: 4612 4701 4765 4970 5083 5303 6045 6130 7201
Size: 89 64 205 113 220 742 85 1071
Cuts at: 7201 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 560 534 399 298 242
220 211 205 148 141 122 113 109
89 85 64 54 51 47 45 44
43 35 32 32 26 15 12 12
9
BspEI T'CCGG_A
Cuts at: 7647 7647
Size: 7984
BspHI T'CATG_A
Cuts at: 2341 5873 7603 2341
Size: 3532 1730 2722
Fragments arranged by size:
3532 2722 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5339 844
Size: 4495 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 4249 5871 1394
Size: 429 1960 466 1622 3507
Fragments arranged by size:
3507 1960 1622 466 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6479 6653 65
Size: 1183 5231 174 1396
Fragments arranged by size:
5231 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2658 4148 4358 6628 6815 1700
Size: 110 848 1490 210 2270 187 2869
Fragments arranged by size:
2869 2270 1490 848 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7984
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7984
BssSI C'ACGA_G
Cuts at: 2705 2855 6044 7056 2705
Size: 150 3189 1012 3633
Fragments arranged by size:
3633 3189 1012 150
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 3350 4189 4951 5132 794
Size: 1541 1015 839 762 181 3646
Fragments arranged by size:
3646 1541 1015 839 762 181
BstBI TT'CG_AA
Cuts at: 6788 6788
Size: 7984
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7984
BstXI CCAn_nnnn'nTGG
Cuts at: 3515 3742 3887 4642 4993 3515
Size: 227 145 755 351 6506
Fragments arranged by size:
6506 755 351 227 145
BstYI r'GATC_y
Cuts at: 1972 4221 4923 5314 5681 6066 6083 7535 7621
Size: 2249 702 391 367 385 17 1452 86
Cuts at: 7621 7633 7979 1972
Size: 12 346 1977
Fragments arranged by size:
2249 1977 1452 702 391 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3993 3993
Size: 7984
BtgI C'CryG_G
Cuts at: 513 1065 2310 2431 2802 3430 3897 4641 4980
Size: 552 1245 121 371 628 467 744 339
Cuts at: 4980 513
Size: 3517
Fragments arranged by size:
3517 1245 744 628 552 467 371 339
121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 6377
Fragments arranged by size:
6377 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 2811 2959 3528 4681 5175 5227 5500 6294
Size: 173 148 569 1153 494 52 273 794
Cuts at: 6294 6314 2638
Size: 20 4308
Fragments arranged by size:
4308 1153 794 569 494 273 173 148
52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2096 2131 529
Size: 35 1532 35 6382
Fragments arranged by size:
6382 1532 35 35
DraI TTT'AAA
Cuts at: 5288 5288
Size: 7984
DraIII CAC_nnn'GTG
Cuts at: 2263 3350 4067 2263
Size: 1087 717 6180
Fragments arranged by size:
6180 1087 717
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6991 809
Size: 507 5675 1802
Fragments arranged by size:
5675 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 4075 4236
Size: 54 1425 50 275 18 270 1975 161
Cuts at: 4236 4983 5121 5431 6320 7949 8
Size: 747 138 310 889 1629 43
Fragments arranged by size:
1975 1629 1425 889 747 310 275 270
161 138 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5431 1537
Size: 275 3619 4090
Fragments arranged by size:
4090 3619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 2717 2848 2995 3010 3817 4046 5914
Size: 872 492 131 147 15 807 229 1868
Cuts at: 5914 1353
Size: 3423
Fragments arranged by size:
3423 1868 872 807 492 229 147 131
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2601 2971 5175 6571 6945 7091
Size: 1064 138 1012 370 2204 1396 374 146
Cuts at: 7091 387
Size: 1280
Fragments arranged by size:
2204 1396 1280 1064 1012 374 370 146
138
EcoICRI GAG'CTC
Cuts at: 3057 3057
Size: 7984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2530 2619 3107 3120 3229 3413
Size: 393 890 8 89 488 13 109 184
Cuts at: 3413 3570 3653 3718 3977 4145 4193 4508 4808
Size: 157 83 65 259 168 48 315 300
Cuts at: 4808 4829 4838 5097 5151 6047 6644 7431 7699
Size: 21 9 259 54 896 597 787 268
Cuts at: 7699 1239
Size: 1524
Fragments arranged by size:
1524 896 890 787 597 488 393 315
300 268 259 259 184 168 157 109
89 83 65 54 48 21 13 9
8
EcoNI CCTnn'n_nnAGG
Cuts at: 3384 3384
Size: 7984
EcoO109I rG'GnC_Cy
Cuts at: 2144 3023 3024 3312 3478 3676 3746 3747 3756
Size: 879 1 288 166 198 70 1 9
Cuts at: 3756 3984 4335 4447 4563 4564 4681 4761 4762
Size: 228 351 112 116 1 117 80 1
Cuts at: 4762 2144
Size: 5366
Fragments arranged by size:
5366 879 351 288 228 198 166 117
116 112 80 70 9 1 1 1
1
EcoRI G'AATT_C
Cuts at: 5293 5671 5293
Size: 378 7606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2762 3217 2762
Size: 455 7529
FspI TGC'GCA
Cuts at: 6490 6490
Size: 7984
HaeII r_GCGC'y
Cuts at: 1678 2598 3269 4181 4951 7131 1678
Size: 920 671 912 770 2180 2531
Fragments arranged by size:
2531 2180 920 912 770 671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2810 2842 3095 3127 3272
Size: 32 135 32 1238 32 253 32 145
Cuts at: 3272 3304 3668 3700 3976 3989 4008 4021 4439
Size: 32 364 32 276 13 19 13 418
Cuts at: 4439 4471 5154 5186 6625 6657 6699 6731 1373
Size: 32 683 32 1439 32 42 32 2626
Fragments arranged by size:
2626 1439 1238 683 418 364 276 253
145 135 42 32 32 32 32 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2441 4156 5328 5574 669
Size: 988 281 503 1715 1172 246 3079
Fragments arranged by size:
3079 1715 1172 988 503 281 246
HindIII A'AGCT_T
Cuts at: 748 2112 2445 4741 748
Size: 1364 333 2296 3991
Fragments arranged by size:
3991 2296 1364 333
HpaI GTT'AAC
Cuts at: 5574 5574
Size: 7984
KasI G'GCGC_C
Cuts at: 3265 3265
Size: 7984
KpnI G_GTAC'C
Cuts at: 5031 5309 5031
Size: 278 7706
MfeI C'AATT_G
Cuts at: 5583 5583
Size: 7984
MluI A'CGCG_T
Cuts at: 2088 7860 2088
Size: 5772 2212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7098 7282 1305
Size: 5793 184 2007
Fragments arranged by size:
5793 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4985 10
Size: 54 1425 343 3153 3009
Fragments arranged by size:
3153 3009 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 3836 4640 5942 6301 6460 518
Size: 648 2193 477 804 1302 359 159 2042
Fragments arranged by size:
2193 2042 1302 804 648 477 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2577 3485 3511 4207 4513 4622 4724
Size: 462 509 908 26 696 306 109 102
Cuts at: 4724 4777 4879 4975 5014 5075 5132 5138 5249
Size: 53 102 96 39 61 57 6 111
Cuts at: 5249 5400 6077 7225 7470 1606
Size: 151 677 1148 245 2120
Fragments arranged by size:
2120 1148 908 696 677 509 462 306
245 151 111 109 102 102 96 61
57 53 39 26 6
NaeI GCC'GGC
Cuts at: 4150 4360 4150
Size: 210 7774
NarI GG'CG_CC
Cuts at: 3266 3266
Size: 7984
NcoI C'CATG_G
Cuts at: 513 1065 2310 2431 3430 3897 4641 4980 513
Size: 552 1245 121 999 467 744 339 3517
Fragments arranged by size:
3517 1245 999 744 552 467 339 121
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7984
NgoMIV G'CCGG_C
Cuts at: 4148 4358 4148
Size: 210 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7984
NotI GC'GGCC_GC
Cuts at: 5431 5431
Size: 7984
NspI r_CATG'y
Cuts at: 1132 1229 5342 6887 1132
Size: 97 4113 1545 2229
Fragments arranged by size:
4113 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3275 3752 4097 4331 1348
Size: 1927 477 345 234 5001
Fragments arranged by size:
5001 1927 477 345 234
PciI A'CATG_T
Cuts at: 6883 6883
Size: 7984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7984
PfoI T'CCnGG_A
Cuts at: 4396 4396
Size: 7984
PmeI GTTT'AAAC
Cuts at: 5288 5288
Size: 7984
PmlI CAC'GTG
Cuts at: 2260 2260
Size: 7984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6053 6085 7566 7598 6053
Size: 32 1481 32 6439
Fragments arranged by size:
6439 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2144 3676 3756 3984 4447 2144
Size: 1532 80 228 463 5681
Fragments arranged by size:
5681 1532 463 228 80
PshAI GACnn'nnGTC
Cuts at: 1613 4901 1613
Size: 3288 4696
PsiI TTA'TAA
Cuts at: 5554 5554
Size: 7984
PspOMI G'GGCC_C
Cuts at: 3023 3215 3258 3478 3746 4334 4563 4761 3023
Size: 192 43 220 268 588 229 198 6246
Fragments arranged by size:
6246 588 268 229 220 198 192 43
PstI C_TGCA'G
Cuts at: 830 2331 3040 3054 3719 3968 4194 4257 4776
Size: 1501 709 14 665 249 226 63 519
Cuts at: 4776 4947 4956 5137 5143 5336 830
Size: 171 9 181 6 193 3478
Fragments arranged by size:
3478 1501 709 665 519 249 226 193
181 171 63 14 9 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7984
PvuII CAG'CTG
Cuts at: 3485 3511 4777 4879 4975 5132 5138 3485
Size: 26 1266 102 96 157 6 6331
Fragments arranged by size:
6331 1266 157 102 96 26 6
SacI G_AGCT'C
Cuts at: 3059 3059
Size: 7984
SalI G'TCGA_C
Cuts at: 1936 2439 5326 1936
Size: 503 2887 4594
Fragments arranged by size:
4594 2887 503
SanDI GG'GwC_CC
Cuts at: 2144 2144
Size: 7984
SbfI CC_TGCA'GG
Cuts at: 5336 5336
Size: 7984
ScaI AGT'ACT
Cuts at: 2522 3110 6232 7944 2522
Size: 588 3122 1712 2562
Fragments arranged by size:
3122 2562 1712 588
SfcI C'TryA_G
Cuts at: 826 1045 2327 3036 3050 3715 3964 4190 4253
Size: 219 1282 709 14 665 249 226 63
Cuts at: 4253 4772 4943 4952 5133 5139 5332 6467 7148
Size: 519 171 9 181 6 193 1135 681
Cuts at: 7148 7339 826
Size: 191 1471
Fragments arranged by size:
1471 1282 1135 709 681 665 519 249
226 219 193 191 181 171 63 14
9 6
SfoI GGC'GCC
Cuts at: 3267 3267
Size: 7984
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7984
SmaI CCC'GGG
Cuts at: 1872 3263 4615 5311 1872
Size: 1391 1352 696 4545
Fragments arranged by size:
4545 1391 1352 696
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 3625 4375 4744 6088 6989
Size: 197 926 804 878 750 369 1344 901
Cuts at: 6989 7251 820
Size: 262 1553
Fragments arranged by size:
1553 1344 926 901 878 804 750 369
262 197
SnaBI TAC'GTA
Cuts at: 493 5775 493
Size: 5282 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7984
SphI G_CATG'C
Cuts at: 1132 5342 1132
Size: 4210 3774
SrfI GCCC'GGGC
Cuts at: 3263 3263
Size: 7984
SspI AAT'ATT
Cuts at: 5 52 5908 5
Size: 47 5856 2081
Fragments arranged by size:
5856 2081 47
StuI AGG'CCT
Cuts at: 1517 3390 1517
Size: 1873 6111
StyI C'CwwG_G
Cuts at: 513 1065 2310 2431 3171 3430 3447 3897 4641
Size: 552 1245 121 740 259 17 450 744
Cuts at: 4641 4702 4980 5423 513
Size: 61 278 443 3074
Fragments arranged by size:
3074 1245 744 740 552 450 443 278
259 121 61 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 3341 3890 6011 6028 6181 6366
Size: 1255 1134 627 549 2121 17 153 185
Cuts at: 6366 6811 325
Size: 445 1498
Fragments arranged by size:
2121 1498 1255 1134 627 549 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3108 6230
Size: 274 80 33 51 1443 543 588 3122
Cuts at: 6230 7942 96
Size: 1712 138
Fragments arranged by size:
3122 1712 1443 588 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5070 5102 7944 7976 5070
Size: 32 2842 32 5078
Fragments arranged by size:
5078 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2513 2822 1316
Size: 1197 309 6478
Fragments arranged by size:
6478 1197 309
XbaI T'CTAG_A
Cuts at: 5320 5320
Size: 7984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2117 2145 3508 3628 4546 2011
Size: 106 28 1363 120 918 5449
Fragments arranged by size:
5449 1363 918 120 106 28
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7984
XmaI C'CCGG_G
Cuts at: 1870 3261 4613 5309 1870
Size: 1391 1352 696 4545
Fragments arranged by size:
4545 1391 1352 696
XmnI GAAnn'nnTTC
Cuts at: 6113 6113
Size: 7984
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5792 276
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SexAI SfiI
SwaI