Restriction map for
pFN21AB4327
(Circular) (Six-base) MAPSORT of: pFN21AB4327.seq Check: 4840 from: 1 to: 6319
pFN21AB4327 6319 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 2290
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2520 3662 1937
Size: 583 1142 4594
Fragments arranged by size:
4594 1142 583
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4382 5766 6034 1632
Size: 2750 1384 268 1917
Fragments arranged by size:
2750 1917 1384 268
AfeI AGC'GCT
Cuts at: 2125 2125
Size: 6319
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3494 3521 1184
Size: 2310 27 3982
Fragments arranged by size:
3982 2310 27
AlwNI CAG_nnn'CTG
Cuts at: 2812 2845 4062 5634 2812
Size: 33 1217 1572 3497
Fragments arranged by size:
3497 1572 1217 33
ApaI G_GGCC'C
Cuts at: 2423 2423
Size: 6319
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3509 3644 1870
Size: 73 1566 135 4545
Fragments arranged by size:
4545 1566 135 73
BamHI G'GATC_C
Cuts at: 2775 3649 4016 2775
Size: 874 367 5078
Fragments arranged by size:
5078 874 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 3041 3640 5095 618
Size: 325 217 627 245 1009 599 1455 1842
Fragments arranged by size:
1842 1455 1009 627 599 325 245 217
BanII G_rGCy'C
Cuts at: 1391 2423 3005 3199 3264 3638 1391
Size: 1032 582 194 65 374 4072
Fragments arranged by size:
4072 1032 582 374 194 65
BbeI G_GCGC'C
Cuts at: 2036 2036
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3055 928
Size: 971 1156 4192
Fragments arranged by size:
4192 1156 971
BbvCI CC'TCA_GC
Cuts at: 2771 3290 3309 2771
Size: 519 19 5781
Fragments arranged by size:
5781 519 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2170 2204 4510 4544 1903
Size: 34 233 34 2306 34 3678
Fragments arranged by size:
3678 2306 233 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 2661 3351 1548
Size: 1113 690 4516
Fragments arranged by size:
4516 1113 690
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2803 2831 3099
Size: 107 122 71 631 605 1131 28 268
Cuts at: 3099 4930 136
Size: 1831 1525
Fragments arranged by size:
1831 1525 1131 631 605 268 122 107
71 28
BglII A'GATC_T
Cuts at: 3220 6314 3220
Size: 3094 3225
BlpI GC'TnA_GC
Cuts at: 2784 3736 2784
Size: 952 5367
Bme1580I G_kGCm'C
Cuts at: 1790 2423 2604 4380 5536 1790
Size: 633 181 1776 1156 2573
Fragments arranged by size:
2573 1776 1156 633 181
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4050 5008 442
Size: 416 53 83 399 24 2633 958 1753
Fragments arranged by size:
2633 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2555 2735 2867 4979 1239
Size: 1091 225 180 132 2112 2579
Fragments arranged by size:
2579 2112 1091 225 180 132
Bpu10I CC'TnA_GC
Cuts at: 2771 3290 3309 2771
Size: 519 19 5781
Fragments arranged by size:
5781 519 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 4444 5309 5607 2201
Size: 2243 865 298 2913
Fragments arranged by size:
2913 2243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3482 4982 882
Size: 617 1983 1500 2219
Fragments arranged by size:
2219 1983 1500 617
BsaAI yAC'GTr
Cuts at: 493 1204 3433 4110 493
Size: 711 2229 677 2702
Fragments arranged by size:
2702 2229 711 677
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 2109 2463 4126
Size: 53 83 186 936 500 76 354 1663
Cuts at: 4126 4508 5143 275
Size: 382 635 1451
Fragments arranged by size:
1663 1451 936 635 500 382 354 186
83 76 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2747 2777 1178
Size: 30 165 30 1344 30 4720
Fragments arranged by size:
4720 1344 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 2768 2834 2888 3006 3009 3259 3446
Size: 1257 325 66 54 118 3 250 187
Cuts at: 3446 1186
Size: 4059
Fragments arranged by size:
4059 1257 325 250 187 118 66 54
3
BseYI C'CCAG_C
Cuts at: 2082 2812 3151 3393 5522 6018 2082
Size: 730 339 242 2129 496 2383
Fragments arranged by size:
2383 2129 730 496 339 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2200 1163
Size: 573 464 5282
Fragments arranged by size:
5282 573 464
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 3026 3519 4380 4465 5536 2250
Size: 776 493 861 85 1071 3033
Fragments arranged by size:
3033 1071 861 776 493 85
BsmI GAATG_Cn'
Cuts at: 3462 3830 3923 3462
Size: 368 93 5858
Fragments arranged by size:
5858 368 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2423 2604 3005 3026 3199 3264
Size: 399 460 173 181 401 21 173 65
Cuts at: 3264 3519 3638 4380 4465 5536 1391
Size: 255 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 401 399 255 181
173 173 119 85 65 21
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 2864 3674 844
Size: 1446 574 810 3489
Fragments arranged by size:
3489 1446 810 574
BsrBI CCG'CTC
Cuts at: 1394 1823 2669 3384 4206 1394
Size: 429 846 715 822 3507
Fragments arranged by size:
3507 846 822 715 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2044 2209 2952 3132 4814 4988 65
Size: 1183 796 165 743 180 1682 174 1396
Fragments arranged by size:
1682 1396 1183 796 743 180 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 2501 2793 3128 3540 4963 5150 1700
Size: 110 691 292 335 412 1423 187 2869
Fragments arranged by size:
2869 1423 691 412 335 292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 2176 4379 5391 2176
Size: 2203 1012 3104
Fragments arranged by size:
3104 2203 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3436 794
Size: 2642 3677
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 2360 3073 1184
Size: 1176 713 4430
Fragments arranged by size:
4430 1176 713
BstYI r'GATC_y
Cuts at: 1972 2275 2316 2775 3220 3649 4016 4401 4418
Size: 303 41 459 445 429 367 385 17
Cuts at: 4418 5870 5956 5968 6314 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 459 445 429 385 367 346
303 86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2800 3078 3337 3412 513
Size: 552 939 796 278 259 75 3420
Fragments arranged by size:
3420 939 796 552 278 259 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3835 4629 4649 2971
Size: 864 794 20 4641
Fragments arranged by size:
4641 864 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 3192 3436 3192
Size: 244 6075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2516 5326 809
Size: 507 1200 2810 1802
Fragments arranged by size:
2810 1802 1200 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 2380 3033
Size: 54 1425 50 275 18 436 114 653
Cuts at: 3033 3091 3766 4655 6284 8
Size: 58 675 889 1629 43
Fragments arranged by size:
1629 1425 889 675 653 436 275 114
58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 2687 3269 4249 1353
Size: 974 360 582 980 3423
Fragments arranged by size:
3423 980 974 582 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2555 2735 2867 4382 4979 5766
Size: 393 698 225 180 132 1515 597 787
Cuts at: 5766 6034 1239
Size: 268 1524
Fragments arranged by size:
1524 1515 787 698 597 393 268 225
180 132
EcoNI CCTnn'n_nnAGG
Cuts at: 2988 3509 2988
Size: 521 5798
EcoO109I rG'GnC_Cy
Cuts at: 2058 2420 2833 2842 2058
Size: 362 413 9 5535
Fragments arranged by size:
5535 413 362 9
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2036 2127 2145 3371 3400 5466 1678
Size: 358 91 18 1226 29 2066 2531
Fragments arranged by size:
2531 2066 1226 358 91 29 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2653 2685 2747 2779 3343
Size: 32 135 32 1081 32 62 32 564
Cuts at: 3343 3375 4960 4992 5034 5066 1373
Size: 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1585 1081 564 135 62 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2509 3663 3909 669
Size: 988 281 571 1154 246 3079
Fragments arranged by size:
3079 1154 988 571 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 2032 2032
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3035 10
Size: 54 1425 343 1203 3294
Fragments arranged by size:
3294 1425 1203 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4277 4636 4795 518
Size: 648 3111 359 159 2042
Fragments arranged by size:
3111 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2106 2184 3039 3576 3592 3735 4412
Size: 435 65 78 855 537 16 143 677
Cuts at: 4412 5560 5805 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 537 435 245 143
78 65 16
NaeI GCC'GGC
Cuts at: 3542 3542
Size: 6319
NarI GG'CG_CC
Cuts at: 2033 2033
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 2800 3412 513
Size: 552 939 796 612 3420
Fragments arranged by size:
3420 939 796 612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 3540 3540
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3677 5222 1132
Size: 97 2448 1545 2229
Fragments arranged by size:
2448 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 3192 1108
Size: 1878 206 4235
Fragments arranged by size:
4235 1878 206
PfoI T'CCnGG_A
Cuts at: 2307 2712 3519 2307
Size: 405 807 5107
Fragments arranged by size:
5107 807 405
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 3433 3433
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2842 2842
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2419 2419
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2449 2841 3429 3671 830
Size: 1619 392 588 242 3478
Fragments arranged by size:
3478 1619 588 392 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 3269 3269
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 2841 3671 2841
Size: 830 5489
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 2445 2651 2837 3425 3667 4802 5483
Size: 219 1400 206 186 588 242 1135 681
Cuts at: 5483 5674 826
Size: 191 1471
Fragments arranged by size:
1471 1400 1135 681 588 242 219 206
191 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 2831 2831
Size: 6319
SfoI GGC'GCC
Cuts at: 2034 2034
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 4423 5324 5586 820
Size: 197 926 273 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 273 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 2800 3256 3412 3758 513
Size: 552 939 402 394 456 156 346 3074
Fragments arranged by size:
3074 939 552 456 402 394 346 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3099 4346 4363 4516 4701 5146
Size: 1255 924 595 1247 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1498 1255 1247 924 595 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 6279 6311 2267
Size: 32 3980 32 2275
Fragments arranged by size:
3980 2275 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2464 3410 2026
Size: 438 946 4935
Fragments arranged by size:
4935 946 438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SrfI SwaI