Restriction map for
pFN21AB4303
(Circular) (Six-base) MAPSORT of: pFN21AB4303.seq Check: 8770 from: 1 to: 6610
pFN21AB4303 6610 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3219 3743 3219
Size: 524 6086
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2378 3387 4673 6057 6325 1632
Size: 644 102 1009 1286 1384 268 1917
Fragments arranged by size:
1917 1384 1286 1009 644 268 102
AflII C'TTAA_G
Cuts at: 820 1017 2216 820
Size: 197 1199 5214
Fragments arranged by size:
5214 1199 197
AflIII A'CryG_T
Cuts at: 1203 2397 3313 3565 5509 6486 1203
Size: 1194 916 252 1944 977 1327
Fragments arranged by size:
1944 1327 1194 977 916 252
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 2746 5339 2746
Size: 2593 4017
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6610
AlwNI CAG_nnn'CTG
Cuts at: 3386 3404 3472 4353 5925 3386
Size: 18 68 881 1572 4071
Fragments arranged by size:
4071 1572 881 68 18
ApaI G_GGCC'C
Cuts at: 2805 2805
Size: 6610
ApaLI G'TGCA_C
Cuts at: 2591 2609 4667 5823 2591
Size: 18 2058 1156 3378
Fragments arranged by size:
3378 2058 1156 18
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 2804 2964 3642 3935 1870
Size: 73 810 51 160 678 293 4545
Fragments arranged by size:
4545 810 678 293 160 73 51
AvrII C'CTAG_G
Cuts at: 2078 3757 2078
Size: 1679 4931
BamHI G'GATC_C
Cuts at: 3652 3940 4307 3652
Size: 288 367 5955
Fragments arranged by size:
5955 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2732 3113 3875 3931 5386
Size: 325 217 627 945 381 762 56 1455
Cuts at: 5386 618
Size: 1842
Fragments arranged by size:
1842 1455 945 762 627 381 325 217
56
BanII G_rGCy'C
Cuts at: 1391 2202 2805 3156 3339 3432 3929 1391
Size: 811 603 351 183 93 497 4072
Fragments arranged by size:
4072 811 603 497 351 183 93
BbeI G_GCGC'C
Cuts at: 3879 3879
Size: 6610
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5639
BbvCI CC'TCA_GC
Cuts at: 3405 3405
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 5221 136
Size: 107 122 71 631 605 874 2675 1525
Fragments arranged by size:
2675 1525 874 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6605 6605
Size: 6610
BlpI GC'TnA_GC
Cuts at: 2513 4027 2513
Size: 1514 5096
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2595 2613 2735 2805 3090 3700 4671
Size: 362 443 18 122 70 285 610 971
Cuts at: 4671 5827 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 971 610 443 362 285 122
70 18
BmgBI CAC'GTC
Cuts at: 1318 2398 3568 1318
Size: 1080 1170 4360
Fragments arranged by size:
4360 1170 1080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3073 3630 4341
Size: 416 53 83 399 24 1656 557 711
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
1753 1656 958 711 557 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 3308 3353 3416 3464 5270 1239
Size: 889 1180 45 63 48 1806 2579
Fragments arranged by size:
2579 1806 1180 889 63 48 45
Bpu10I CC'TnA_GC
Cuts at: 3405 3473 3405
Size: 68 6542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 3326 4735 5600 5898 2672
Size: 654 1409 865 298 3384
Fragments arranged by size:
3384 1409 865 654 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2882 3077 5273 882
Size: 617 1383 195 2196 2219
Fragments arranged by size:
2219 2196 1383 617 195
BsaAI yAC'GTr
Cuts at: 493 1204 2488 2608 3316 4401 493
Size: 711 1284 120 708 1085 2702
Fragments arranged by size:
2702 1284 1085 711 708 120
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2861 3876 4417 4799
Size: 53 83 186 936 1328 1015 541 382
Cuts at: 4799 5434 275
Size: 635 1451
Fragments arranged by size:
1451 1328 1015 936 635 541 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3033 3100 5043 5441 5715 5862 6273 3033
Size: 67 1943 398 274 147 411 3370
Fragments arranged by size:
3370 1943 411 398 274 147 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3158 3188 3218 3248 1178
Size: 30 165 30 1755 30 30 30 4540
Fragments arranged by size:
4540 1755 165 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2972 3461 3464 1186
Size: 1216 570 489 3 4332
Fragments arranged by size:
4332 1216 570 489 3
BseYI C'CCAG_C
Cuts at: 2191 2824 3007 3326 5813 6309 2191
Size: 633 183 319 2487 496 2492
Fragments arranged by size:
2492 2487 633 496 319 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 1163
Size: 573 684 5353
Fragments arranged by size:
5353 684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2541 2717 3680 4060 4821 5849
Size: 275 186 540 176 963 380 761 1028
Cuts at: 5849 1540
Size: 2301
Fragments arranged by size:
2301 1028 963 761 540 380 275 186
176
BsiHKAI G_wGCw'C
Cuts at: 2595 2613 2922 3432 3459 4671 4756 5827 2595
Size: 18 309 510 27 1212 85 1071 3378
Fragments arranged by size:
3378 1212 1071 510 309 85 27 18
BsmI GAATG_Cn'
Cuts at: 4121 4214 4121
Size: 93 6517
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2202 2595 2613 2735 2805 2922
Size: 399 362 50 393 18 122 70 117
Cuts at: 2922 3090 3156 3339 3432 3459 3700 3929 4671
Size: 168 66 183 93 27 241 229 742
Cuts at: 4671 4756 5827 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 362 241 229
183 168 122 117 93 85 70 66
50 27 18
BspEI T'CCGG_A
Cuts at: 3033 6273 3033
Size: 3240 3370
BspHI T'CATG_A
Cuts at: 2337 4499 6229 2337
Size: 2162 1730 2718
Fragments arranged by size:
2718 2162 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3219 3743 3965 844
Size: 1664 711 524 222 3489
Fragments arranged by size:
3489 1664 711 524 222
BsrBI CCG'CTC
Cuts at: 1394 1823 2530 3586 4497 1394
Size: 429 707 1056 911 3507
Fragments arranged by size:
3507 1056 911 707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5105 5279 65
Size: 1183 3857 174 1396
Fragments arranged by size:
3857 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 2595 3853 5254 5441 1700
Size: 110 605 180 1258 1401 187 2869
Fragments arranged by size:
2869 1401 1258 605 187 180 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 2549 1925
Size: 624 5986
BssSI C'ACGA_G
Cuts at: 3812 4670 5682 3812
Size: 858 1012 4740
Fragments arranged by size:
4740 1012 858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6610
BstBI TT'CG_AA
Cuts at: 3108 5414 3108
Size: 2306 4304
BstEII G'GTnAC_C
Cuts at: 1184 2159 1184
Size: 975 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 3280 3286 3280
Size: 6 6604
BstYI r'GATC_y
Cuts at: 1972 2757 3652 3940 4307 4692 4709 6161 6247
Size: 785 895 288 367 385 17 1452 86
Cuts at: 6247 6259 6605 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 895 785 385 367 346 288
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2955 3248 2955
Size: 293 6317
BtgI C'CryG_G
Cuts at: 513 1065 2004 2880 3162 3279 3648 3680 513
Size: 552 939 876 282 117 369 32 3443
Fragments arranged by size:
3443 939 876 552 369 282 117 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5871
Fragments arranged by size:
5871 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 3156 4126 4920 4940 2507
Size: 649 970 794 20 4177
Fragments arranged by size:
4177 970 794 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2948 3260 5617 809
Size: 507 1632 312 2357 1802
Fragments arranged by size:
2357 1802 1632 507 312
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2538 2577 2653
Size: 54 1425 50 275 18 708 39 76
Cuts at: 2653 2667 3677 3855 4057 4946 6575 8
Size: 14 1010 178 202 889 1629 43
Fragments arranged by size:
1629 1425 1010 889 708 275 202 178
76 54 50 43 39 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 2538 3677 4057 1537
Size: 275 726 1139 380 4090
Fragments arranged by size:
4090 1139 726 380 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 4540 1353
Size: 1001 2186 3423
Fragments arranged by size:
3423 2186 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2579 2651 2900 5197 5571 5717
Size: 1064 138 990 72 249 2297 374 146
Cuts at: 5717 387
Size: 1280
Fragments arranged by size:
2297 1280 1064 990 374 249 146 138
72
EcoICRI GAG'CTC
Cuts at: 3430 3430
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2276 2378 3308 3353 3387 3416
Size: 393 496 148 102 930 45 34 29
Cuts at: 3416 3464 4673 5270 6057 6325 1239
Size: 48 1209 597 787 268 1524
Fragments arranged by size:
1524 1209 930 787 597 496 393 268
148 102 48 45 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2787 3446 3772 2787
Size: 659 326 5625
Fragments arranged by size:
5625 659 326
EcoO109I rG'GnC_Cy
Cuts at: 2497 2950 3262 3269 3553 3631 3715 3753 2497
Size: 453 312 7 284 78 84 38 5354
Fragments arranged by size:
5354 453 312 284 84 78 38 7
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 2825 3326 3781 3879 5757 1678
Size: 1147 501 455 98 1878 2531
Fragments arranged by size:
2531 1878 1147 501 455 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 2732 2764 3158
Size: 32 135 32 1091 32 37 32 394
Cuts at: 3158 3190 5251 5283 5325 5357 1373
Size: 32 2061 32 42 32 2626
Fragments arranged by size:
2626 2061 1091 394 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3524 3954 4200 669
Size: 988 281 1586 430 246 3079
Fragments arranged by size:
3079 1586 988 430 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KasI G'GCGC_C
Cuts at: 3875 3875
Size: 6610
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2251 3156 5724 5908 1305
Size: 946 905 2568 184 2007
Fragments arranged by size:
2568 2007 946 905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 2655 10
Size: 54 1425 343 747 76 3965
Fragments arranged by size:
3965 1425 747 343 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3161 3278 4568 4927 5086 518
Size: 648 1041 954 117 1290 359 159 2042
Fragments arranged by size:
2042 1290 1041 954 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2195 2434 2649 2935 2980 3301 3365 3383
Size: 589 239 215 286 45 321 64 18
Cuts at: 3383 3620 3702 4026 4703 5851 6096 1606
Size: 237 82 324 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 324 321 286 245
239 237 215 82 64 45 18
NaeI GCC'GGC
Cuts at: 3855 3855
Size: 6610
NarI GG'CG_CC
Cuts at: 3876 3876
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 2004 3162 3279 513
Size: 552 939 1158 117 3844
Fragments arranged by size:
3844 1158 939 552 117
NdeI CA'TA_TG
Cuts at: 387 3666 387
Size: 3279 3331
NgoMIV G'CCGG_C
Cuts at: 3853 3853
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 2356 3129 3968 5513 1132
Size: 97 1127 773 839 1545 2229
Fragments arranged by size:
2229 1545 1127 839 773 97
PasI CC'CwG_GG
Cuts at: 1348 2061 3825 1348
Size: 713 1764 4133
Fragments arranged by size:
4133 1764 713
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6610
PfoI T'CCnGG_A
Cuts at: 2448 3075 3432 2448
Size: 627 357 5626
Fragments arranged by size:
5626 627 357
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PmlI CAC'GTG
Cuts at: 3316 3316
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2950 3262 3553 3631 3753 2497
Size: 453 312 291 78 122 5354
Fragments arranged by size:
5354 453 312 291 122 78
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PspOMI G'GGCC_C
Cuts at: 2801 2801
Size: 6610
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2450 2482 2450
Size: 32 6578
PstI C_TGCA'G
Cuts at: 830 2235 2766 3603 3962 830
Size: 1405 531 837 359 3478
Fragments arranged by size:
3478 1405 837 531 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2434 3301 3365 3620 2434
Size: 867 64 255 5424
Fragments arranged by size:
5424 867 255 64
SacI G_AGCT'C
Cuts at: 3432 3432
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SanDI GG'GwC_CC
Cuts at: 2950 3631 3753 2950
Size: 681 122 5807
Fragments arranged by size:
5807 681 122
SbfI CC_TGCA'GG
Cuts at: 2766 3962 2766
Size: 1196 5414
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SexAI A'CCwGG_T
Cuts at: 2469 2469
Size: 6610
SfcI C'TryA_G
Cuts at: 826 1045 2231 2762 3599 3958 5093 5774 5965
Size: 219 1186 531 837 359 1135 681 191
Cuts at: 5965 826
Size: 1471
Fragments arranged by size:
1471 1186 1135 837 681 531 359 219
191
SfoI GGC'GCC
Cuts at: 3877 3877
Size: 6610
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 3644 3937 1872
Size: 1772 293 4545
Fragments arranged by size:
4545 1772 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2687 2964 3305 4714 5615
Size: 197 926 273 471 277 341 1409 901
Cuts at: 5615 5877 820
Size: 262 1553
Fragments arranged by size:
1553 1409 926 901 471 341 277 273
262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3968 1132
Size: 2836 3774
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 3762 1517
Size: 2245 4365
StyI C'CwwG_G
Cuts at: 513 1065 2004 2078 3162 3279 3757 4049 513
Size: 552 939 74 1084 117 478 292 3074
Fragments arranged by size:
3074 1084 939 552 478 292 117 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2816 3292 4637 4654 4807 4992 5437
Size: 1255 1236 476 1345 17 153 185 445
Cuts at: 5437 325
Size: 1498
Fragments arranged by size:
1498 1345 1255 1236 476 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4856 6568 96
Size: 274 80 33 51 1443 2879 1712 138
Fragments arranged by size:
2879 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 6570 6602 2405
Size: 32 4133 32 2413
Fragments arranged by size:
4133 2413 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3172 1316
Size: 1856 4754
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XhoI C'TCGA_G
Cuts at: 1943 2964 1943
Size: 1021 5589
XmaI C'CCGG_G
Cuts at: 1870 3642 3935 1870
Size: 1772 293 4545
Fragments arranged by size:
4545 1772 293
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI RsrII SacII SapI SfiI SrfI SwaI XcmI