Restriction map for
pFN21AB4280
(Circular) (Six-base) MAPSORT of: pFN21AB4280.seq Check: 1173 from: 1 to: 5518
pFN21AB4280 5518 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2592 2839 2592
Size: 247 5271
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 3581 4965 5233 1632
Size: 920 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 2698 4417 5394 1203
Size: 1495 1719 977 1327
Fragments arranged by size:
1719 1495 1327 977
AgeI A'CCGG_T
Cuts at: 2589 4349 2589
Size: 1760 3758
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 2495 1166
Size: 1329 4189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaI G_GGCC'C
Cuts at: 2798 2817 2798
Size: 19 5499
ApaLI G'TGCA_C
Cuts at: 2725 3575 4731 2725
Size: 850 1156 3512
Fragments arranged by size:
3512 1156 850
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 2843 1870
Size: 73 267 633 4545
Fragments arranged by size:
4545 633 267 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2592 2839 4294 618
Size: 325 217 627 805 247 1455 1842
Fragments arranged by size:
1842 1455 805 627 325 247 217
BanII G_rGCy'C
Cuts at: 1391 2211 2798 2817 2837 1391
Size: 820 587 19 20 4072
Fragments arranged by size:
4072 820 587 20 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2560 928
Size: 971 661 3886
Fragments arranged by size:
3886 971 661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3709 3743 1903
Size: 34 50 34 1688 34 3678
Fragments arranged by size:
3678 1688 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 4129 136
Size: 107 122 71 631 605 440 2017 1525
Fragments arranged by size:
2017 1525 631 605 440 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2729 2798 2817 3579 4735 1790
Size: 939 69 19 762 1156 2573
Fragments arranged by size:
2573 1156 939 762 69 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 3249 4207
Size: 416 53 83 399 24 681 1151 958
Cuts at: 4207 442
Size: 1753
Fragments arranged by size:
1753 1151 958 681 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2363 2795 4178 1239
Size: 1013 111 432 1383 2579
Fragments arranged by size:
2579 1383 1013 432 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2701 3309 493
Size: 711 1497 608 2702
Fragments arranged by size:
2702 1497 711 608
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2307 3325 3707 4342
Size: 53 83 186 936 774 1018 382 635
Cuts at: 4342 275
Size: 1451
Fragments arranged by size:
1451 1018 936 774 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2589 3951 4349 4623 4770 5181 2589
Size: 1362 398 274 147 411 2926
Fragments arranged by size:
2926 1362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4351
Fragments arranged by size:
4351 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5518
BseYI C'CCAG_C
Cuts at: 2044 2094 2347 2451 2526 4721 5217 2044
Size: 50 253 104 75 2195 496 2345
Fragments arranged by size:
2345 2195 496 253 104 75 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4945
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2729 3579 3664 4735 2325
Size: 404 850 85 1071 3108
Fragments arranged by size:
3108 1071 850 404 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2325 2729 2798 2817 2837 3579
Size: 399 421 114 404 69 19 20 742
Cuts at: 3579 3664 4735 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 404 399 114 85
69 20 19
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2604 2873 844
Size: 1313 447 269 3489
Fragments arranged by size:
3489 1313 447 269
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2589 4162 4349 1700
Size: 110 779 1573 187 2869
Fragments arranged by size:
2869 1573 779 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 2488 1184
Size: 1304 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2278
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4966
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3828 3848 3034
Size: 794 20 4704
Fragments arranged by size:
4704 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483
Size: 54 1425 50 275 18 1135 889 1629
Cuts at: 5483 8
Size: 43
Fragments arranged by size:
1629 1425 1135 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2363 2552 2795 3581 4178 4965
Size: 393 620 111 189 243 786 597 787
Cuts at: 4965 5233 1239
Size: 268 1524
Fragments arranged by size:
1524 787 786 620 597 393 268 243
189 111
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 2777
Size: 5518
EcoO109I rG'GnC_Cy
Cuts at: 2014 2098 2352 2794 2813 2014
Size: 84 254 442 19 4719
Fragments arranged by size:
4719 442 254 84 19
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 2378 4024 2378
Size: 1646 3872
FspAI rTGC'GCAy
Cuts at: 2378 2378
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373
Size: 32 135 32 2587 32 42 32 2626
Fragments arranged by size:
2626 2587 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2596 2843 2596
Size: 247 5271
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2482 4632 4816 1305
Size: 1177 2150 184 2007
Fragments arranged by size:
2150 2007 1177 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2495 3476 3835 3994 518
Size: 648 1110 219 981 359 159 2042
Fragments arranged by size:
2042 1110 981 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2372 2455 2530 2715 2934 3611 4759
Size: 430 336 83 75 185 219 677 1148
Cuts at: 4759 5004 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 430 336 245 219 185
83 75
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2204 2876 4421 1132
Size: 97 975 672 1545 2229
Fragments arranged by size:
2229 1545 975 672 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 1108
Size: 1350 4168
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PmlI CAC'GTG
Cuts at: 2701 2701
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2794 2813 2794
Size: 19 5499
PstI C_TGCA'G
Cuts at: 830 2136 2199 2870 830
Size: 1306 63 671 3478
Fragments arranged by size:
3478 1306 671 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2036 2372 2530 2715 2036
Size: 336 158 185 4839
Fragments arranged by size:
4839 336 185 158
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2132 2195 2866 4001 4682 4873 826
Size: 219 1087 63 671 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 671 219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2212 2845 1872
Size: 340 633 4545
Fragments arranged by size:
4545 633 340
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2204 2876 1132
Size: 1072 672 3774
Fragments arranged by size:
3774 1072 672
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2166 2957 513
Size: 552 1101 791 3074
Fragments arranged by size:
3074 1101 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 2465 3545 3562 3715 3900 4345
Size: 1255 567 318 1080 17 153 185 445
Cuts at: 4345 325
Size: 1498
Fragments arranged by size:
1498 1255 1080 567 445 318 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 5478 5510 2315
Size: 32 3131 32 2323
Fragments arranged by size:
3131 2323 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 2564 1316
Size: 1170 78 4270
Fragments arranged by size:
4270 1170 78
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2210 2843 1870
Size: 340 633 4545
Fragments arranged by size:
4545 633 340
XmnI GAAnn'nnTTC
Cuts at: 2123 3647 2123
Size: 1524 3994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI