Restriction map for
pFN21AB4278
(Circular) (Six-base) MAPSORT of: pFN21AB4278.seq Check: 7370 from: 1 to: 5737
pFN21AB4278 5737 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 3080 1937
Size: 136 1007 4594
Fragments arranged by size:
4594 1007 136
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 3800 5184 5452 1632
Size: 876 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 876 268
AflII C'TTAA_G
Cuts at: 820 1017 2798 820
Size: 197 1781 3759
Fragments arranged by size:
3759 1781 197
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaLI G'TGCA_C
Cuts at: 2986 3794 4950 2986
Size: 808 1156 3773
Fragments arranged by size:
3773 1156 808
ApoI r'AATT_y
Cuts at: 1493 2679 3046 3272 3424 5533 1493
Size: 1186 367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1186 367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2712 4291 160
Size: 872 1680 1579 1606
Fragments arranged by size:
1680 1606 1579 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2103 3062 1870
Size: 73 114 46 959 4545
Fragments arranged by size:
4545 959 114 73 46
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3056 1391
Size: 1665 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 928
Size: 971 456 4310
Fragments arranged by size:
4310 971 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 2977 1548
Size: 1429 4308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2794 5732 2794
Size: 2938 2799
BlpI GC'TnA_GC
Cuts at: 2565 3154 2565
Size: 589 5148
Bme1580I G_kGCm'C
Cuts at: 1790 2990 3798 4954 1790
Size: 1200 808 1156 2573
Fragments arranged by size:
2573 1200 1156 808
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 3468 4426
Size: 416 53 83 399 24 1550 501 958
Cuts at: 4426 442
Size: 1753
Fragments arranged by size:
1753 1550 958 501 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2755 4397 1239
Size: 1516 1642 2579
Fragments arranged by size:
2579 1642 1516
Bpu10I CC'TnA_GC
Cuts at: 2868 2868
Size: 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2382 4170 4568 4842 4989 5400 2382
Size: 1788 398 274 147 411 2719
Fragments arranged by size:
2719 1788 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2958 2963 1186
Size: 1772 5 3960
Fragments arranged by size:
3960 1772 5
BseYI C'CCAG_C
Cuts at: 4940 5436 4940
Size: 496 5241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2990 3798 3883 4954 2028
Size: 962 808 85 1071 2811
Fragments arranged by size:
2811 1071 962 808 85
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2990 3056 3798 3883 4954 1391
Size: 399 238 962 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 962 742 399 238 85 66
BspEI T'CCGG_A
Cuts at: 2382 5400 2382
Size: 3018 2719
BspHI T'CATG_A
Cuts at: 2038 2371 3626 5356 2038
Size: 333 1255 1730 2419
Fragments arranged by size:
2419 1730 1255 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 3092 844
Size: 1180 1068 3489
Fragments arranged by size:
3489 1180 1068
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2216 2794 3067 3434 3819 3836 5288 5374
Size: 244 578 273 367 385 17 1452 86
Cuts at: 5374 5386 5732 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 578 385 367 346 273 244
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2886 2886
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DraIII CAC_nnn'GTG
Cuts at: 2157 2157
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3184 4073 5702
Size: 54 1425 50 275 18 1354 889 1629
Cuts at: 5702 8
Size: 43
Fragments arranged by size:
1629 1425 1354 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 3667 1353
Size: 729 1585 3423
Fragments arranged by size:
3423 1585 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2755 3800 4397 5184 5452 1239
Size: 393 876 247 1045 597 787 268 1524
Fragments arranged by size:
1524 1045 876 787 597 393 268 247
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4378 4410 4452 4484 1373
Size: 32 135 32 2806 32 42 32 2626
Fragments arranged by size:
2806 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2574 748
Size: 1826 3911
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2074 2122 4851 5035 1305
Size: 769 48 2729 184 2007
Fragments arranged by size:
2729 2007 769 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3695 4054 4213 518
Size: 648 2529 359 159 2042
Fragments arranged by size:
2529 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 3153 3830 4978 5223 1606
Size: 963 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 963 677 584 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5737
PfoI T'CCnGG_A
Cuts at: 2161 2161
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 3089 830
Size: 2259 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
PvuII CAG'CTG
Cuts at: 2569 2569
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2857 3085 4220 4901 5092 826
Size: 219 1812 228 1135 681 191 1471
Fragments arranged by size:
1812 1471 1135 681 228 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2103 2798 3841 4742 5004 820
Size: 197 926 160 695 1043 901 262 1553
Fragments arranged by size:
1553 1043 926 901 695 262 197 160
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 2251 2359 3661 5
Size: 47 2199 108 1302 2081
Fragments arranged by size:
2199 2081 1302 108 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 3176 513
Size: 552 2111 3074
Fragments arranged by size:
3074 2111 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 3781 3934 4119 4564 325
Size: 1255 2184 17 153 185 445 1498
Fragments arranged by size:
2184 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2402 3983 5695
Size: 274 80 33 51 1443 425 1581 1712
Cuts at: 5695 96
Size: 138
Fragments arranged by size:
1712 1581 1443 425 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 5697 5729 2143
Size: 32 3522 32 2151
Fragments arranged by size:
3522 2151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 2103 1943
Size: 160 5577
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI