Restriction map for
pFN21AB4277
(Circular) (Six-base) MAPSORT of: pFN21AB4277.seq Check: 4237 from: 1 to: 5980
pFN21AB4277 5980 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 2847 3267 4043 5427 5695 1632
Size: 945 270 420 776 1384 268 1917
Fragments arranged by size:
1917 1384 945 776 420 270 268
AflII C'TTAA_G
Cuts at: 820 1017 2927 820
Size: 197 1910 3873
Fragments arranged by size:
3873 1910 197
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 3032 4709 3032
Size: 1677 4303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2744 3723 5295 2744
Size: 979 1572 3429
Fragments arranged by size:
3429 1572 979
ApaI G_GGCC'C
Cuts at: 2395 2395
Size: 5980
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2315 3289 3515 3667 5776 1493
Size: 822 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 822 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3305 1870
Size: 73 1362 4545
Fragments arranged by size:
4545 1362 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2401 2434 2401
Size: 33 5947
BamHI G'GATC_C
Cuts at: 3259 3310 3677 3259
Size: 51 367 5562
Fragments arranged by size:
5562 367 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4756 618
Size: 325 217 627 1514 1455 1842
Fragments arranged by size:
1842 1514 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2395 3299 1391
Size: 1004 904 4072
Fragments arranged by size:
4072 1004 904
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2669 3090 3195 3246 928
Size: 971 770 421 105 51 3662
Fragments arranged by size:
3662 971 770 421 105 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2764 3872 5082 2764
Size: 1108 1210 3662
Fragments arranged by size:
3662 1210 1108
BclI T'GATC_A
Cuts at: 1548 2052 2749 1548
Size: 504 697 4779
Fragments arranged by size:
4779 697 504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2580 4041 5197 1790
Size: 605 185 1461 1156 2573
Fragments arranged by size:
2573 1461 1156 605 185
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 3711 4669
Size: 416 53 83 399 24 723 1571 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1571 958 723 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2517 4640 1239
Size: 905 373 2123 2579
Fragments arranged by size:
2579 2123 905 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2419 2449 1178
Size: 30 165 30 1016 30 4709
Fragments arranged by size:
4709 1016 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5980
BseYI C'CCAG_C
Cuts at: 2132 5183 5679 2132
Size: 3051 496 2433
Fragments arranged by size:
3051 2433 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 1163
Size: 573 540 4867
Fragments arranged by size:
4867 573 540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4041 4126 5197 4041
Size: 85 1071 4824
Fragments arranged by size:
4824 1071 85
BsmI GAATG_Cn'
Cuts at: 2190 2935 3491 3584 2190
Size: 745 556 93 4586
Fragments arranged by size:
4586 745 556 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2525 2525
Size: 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2580 3299 4041 4126 5197 1391
Size: 399 605 185 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 605 399 185 85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 2979 1184
Size: 1795 4185
BstYI r'GATC_y
Cuts at: 1972 2488 2908 3259 3310 3677 4062 4079 5531
Size: 516 420 351 51 367 385 17 1452
Cuts at: 5531 5617 5629 5975 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 516 420 385 367 351 346
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2524 513
Size: 552 939 520 3969
Fragments arranged by size:
3969 939 552 520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 4060
Fragments arranged by size:
4060 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 2820 3496 4290 4310 2043
Size: 777 676 794 20 3713
Fragments arranged by size:
3713 794 777 676 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3081 3116 529
Size: 35 2517 35 3393
Fragments arranged by size:
3393 2517 35 35
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316
Size: 54 1425 50 275 18 177 1420 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3910 1353
Size: 2557 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2517 2577 2847 3267 4043 4640
Size: 393 512 373 60 270 420 776 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 776 597 512 420 393 373
270 268 60
EcoO109I rG'GnC_Cy
Cuts at: 2391 2391
Size: 5980
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
EcoRV GAT'ATC
Cuts at: 3135 3135
Size: 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 3018 3050 3082
Size: 32 135 32 532 32 882 32 32
Cuts at: 3082 4621 4653 4695 4727 1373
Size: 1539 32 42 32 2626
Fragments arranged by size:
2626 1539 882 532 135 42 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3203 3938 4297 4456 518
Size: 648 1789 248 735 359 159 2042
Fragments arranged by size:
2042 1789 735 648 359 248 159
MspA1I CmG'CkG
Cuts at: 1606 2885 3396 4073 5221 5466 1606
Size: 1279 511 677 1148 245 2120
Fragments arranged by size:
2120 1279 1148 677 511 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2122 1108
Size: 1014 4966
PfoI T'CCnGG_A
Cuts at: 3146 3146
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2517 3274 1613
Size: 904 757 4319
Fragments arranged by size:
4319 904 757
PsiI TTA'TAA
Cuts at: 3231 3550 3231
Size: 319 5661
PspOMI G'GGCC_C
Cuts at: 2391 2391
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2885 2885
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 3328 4463 5144 5335 826
Size: 219 2283 1135 681 191 1471
Fragments arranged by size:
2283 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2927 4084 4985 5247 820
Size: 197 926 984 1157 901 262 1553
Fragments arranged by size:
1553 1157 984 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 2964 3904 5
Size: 47 2912 940 2081
Fragments arranged by size:
2912 2081 940 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 3419 513
Size: 552 939 1160 255 3074
Fragments arranged by size:
3074 1160 939 552 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3157 4007 4024 4177 4362 4807 325
Size: 1255 1577 850 17 153 185 445 1498
Fragments arranged by size:
1577 1498 1255 850 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 2183 4109 2183
Size: 1926 4054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI