Restriction map for
pFN21AB4274
(Circular) (Six-base) MAPSORT of: pFN21AB4274.seq Check: 4689 from: 1 to: 6646
pFN21AB4274 6646 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2692 3989 1937
Size: 755 1297 4594
Fragments arranged by size:
4594 1297 755
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2562 3025 4709 6093 6361 1632
Size: 633 297 463 1684 1384 268 1917
Fragments arranged by size:
1917 1684 1384 633 463 297 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 5545 6522 1203
Size: 4342 977 1327
Fragments arranged by size:
4342 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 3840 1166
Size: 2674 3972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AlwNI CAG_nnn'CTG
Cuts at: 2071 4389 5961 2071
Size: 2318 1572 2756
Fragments arranged by size:
2756 2318 1572
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 2261 2380 2823 3579 3955 4181 4333 6442
Size: 768 119 443 756 376 226 152 2109
Cuts at: 6442 1493
Size: 1697
Fragments arranged by size:
2109 1697 768 756 443 376 226 152
119
AseI AT'TA_AT
Cuts at: 160 1032 2444 5200 160
Size: 872 1412 2756 1606
Fragments arranged by size:
2756 1606 1412 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 3971 1870
Size: 73 2028 4545
Fragments arranged by size:
4545 2028 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3967 5422 618
Size: 325 217 627 2180 1455 1842
Fragments arranged by size:
2180 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3965 1391
Size: 2574 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 2253 3140 3308 3392 3812 928
Size: 971 194 160 887 168 84 420 3762
Fragments arranged by size:
3762 971 887 420 194 168 160 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 2896 2980 3484 1548
Size: 1348 84 504 4710
Fragments arranged by size:
4710 1348 504 84
BfrBI ATG'CAT
Cuts at: 2870 2870
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5257 136
Size: 107 122 71 631 605 3585 1525
Fragments arranged by size:
3585 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6641 6641
Size: 6646
BlpI GC'TnA_GC
Cuts at: 4063 4063
Size: 6646
Bme1580I G_kGCm'C
Cuts at: 1790 4707 5863 1790
Size: 2917 1156 2573
Fragments arranged by size:
2917 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4377 5335 442
Size: 416 53 83 399 24 2960 958 1753
Fragments arranged by size:
2960 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2604 5306 1239
Size: 1365 2702 2579
Fragments arranged by size:
2702 2579 1365
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2948 4771 5636 5934 2156
Size: 792 1823 865 298 2868
Fragments arranged by size:
2868 1823 865 792 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5309 882
Size: 617 3810 2219
Fragments arranged by size:
3810 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4437 493
Size: 711 3233 2702
Fragments arranged by size:
3233 2702 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4453 4835 5470 275
Size: 53 83 186 936 2920 382 635 1451
Fragments arranged by size:
2920 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5079 5477 5751 5898 6309 5079
Size: 398 274 147 411 5416
Fragments arranged by size:
5416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2602 2632 2996 3026 1178
Size: 30 165 30 1199 30 364 30 4798
Fragments arranged by size:
4798 1199 364 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2199 3353 1186
Size: 886 127 1154 4479
Fragments arranged by size:
4479 1154 886 127
BseYI C'CCAG_C
Cuts at: 5849 6345 5849
Size: 496 6150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6073
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4096 4857 5885 1540
Size: 275 186 2095 761 1028 2301
Fragments arranged by size:
2301 2095 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4707 4792 5863 4707
Size: 85 1071 5490
Fragments arranged by size:
5490 1071 85
BsmI GAATG_Cn'
Cuts at: 3040 4157 4250 3040
Size: 1117 93 5436
Fragments arranged by size:
5436 1117 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3965 4707 4792 5863 1391
Size: 399 2175 742 85 1071 2174
Fragments arranged by size:
2175 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 3319 4535 6265 3319
Size: 1216 1730 3700
Fragments arranged by size:
3700 1730 1216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2201 2228 4001 844
Size: 1357 27 1773 3489
Fragments arranged by size:
3489 1773 1357 27
BsrBI CCG'CTC
Cuts at: 1394 1823 4533 1394
Size: 429 2710 3507
Fragments arranged by size:
3507 2710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2776 2944 3032 5141 5315 65
Size: 1183 1528 168 88 2109 174 1396
Fragments arranged by size:
2109 1528 1396 1183 174 168 88
BsrFI r'CCGG_y
Cuts at: 1700 1810 3671 5290 5477 1700
Size: 110 1861 1619 187 2869
Fragments arranged by size:
2869 1861 1619 187 110
BsrGI T'GTAC_A
Cuts at: 96 3232 3652 3820 96
Size: 3136 420 168 2922
Fragments arranged by size:
3136 2922 420 168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6646
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641
Size: 2004 367 385 17 1452 86 12 346
Cuts at: 6641 1972
Size: 1977
Fragments arranged by size:
2004 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2195 513
Size: 552 939 191 4964
Fragments arranged by size:
4964 939 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 4162 4956 4976 4162
Size: 794 20 5832
Fragments arranged by size:
5832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2758 2793 2926 2961 529
Size: 35 2194 35 133 35 4214
Fragments arranged by size:
4214 2194 133 35 35 35
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3673 4093 4982
Size: 54 1425 50 275 18 1843 420 889
Cuts at: 4982 6611 8
Size: 1629 43
Fragments arranged by size:
1843 1629 1425 889 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2327 2731 3321 4576 1353
Size: 831 143 404 590 1255 3423
Fragments arranged by size:
3423 1255 831 590 404 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2948 3116 3368 3788 5233 5607
Size: 1064 138 1359 168 252 420 1445 374
Cuts at: 5607 5753 387
Size: 146 1280
Fragments arranged by size:
1445 1359 1280 1064 420 374 252 168
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2562 2604 3025 4709 5306 6093
Size: 393 633 297 42 421 1684 597 787
Cuts at: 6093 6361 1239
Size: 268 1524
Fragments arranged by size:
1684 1524 787 633 597 421 393 297
268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2700 3715 2700
Size: 1015 5631
EcoRI G'AATT_C
Cuts at: 2380 2823 3579 3955 4333 2380
Size: 443 756 376 378 4693
Fragments arranged by size:
4693 756 443 378 376
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 5793 1678
Size: 4115 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 2521 2553 2800
Size: 32 135 32 772 32 145 32 247
Cuts at: 2800 2832 5287 5319 5361 5393 1373
Size: 32 2455 32 42 32 2626
Fragments arranged by size:
2626 2455 772 247 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2157 5760 5944 1305
Size: 786 66 3603 184 2007
Fragments arranged by size:
3603 2007 786 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4824
Fragments arranged by size:
4824 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2859 3100 3168 3520 3772 3840
Size: 648 969 724 241 68 352 252 68
Cuts at: 3840 3938 4604 4963 5122 518
Size: 98 666 359 159 2042
Fragments arranged by size:
2042 969 724 666 648 359 352 252
241 159 98 68 68
MspA1I CmG'CkG
Cuts at: 1606 4062 4739 5887 6132 1606
Size: 2456 677 1148 245 2120
Fragments arranged by size:
2456 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2195 513
Size: 552 939 191 4964
Fragments arranged by size:
4964 939 552 191
NdeI CA'TA_TG
Cuts at: 387 3097 3517 387
Size: 2710 420 3516
Fragments arranged by size:
3516 2710 420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NsiI A_TGCA'T
Cuts at: 2872 2872
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2769 4004 5549 1132
Size: 97 1540 1235 1545 2229
Fragments arranged by size:
2229 1545 1540 1235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 3888 4216 3888
Size: 328 6318
PstI C_TGCA'G
Cuts at: 830 2795 3998 830
Size: 1965 1203 3478
Fragments arranged by size:
3478 1965 1203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2184 2731 3321 2184
Size: 547 590 5509
Fragments arranged by size:
5509 590 547
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 2518 4894 6606 2518
Size: 2376 1712 2558
Fragments arranged by size:
2558 2376 1712
SfcI C'TryA_G
Cuts at: 826 1045 2791 3994 5129 5810 6001 826
Size: 219 1746 1203 1135 681 191 1471
Fragments arranged by size:
1746 1471 1203 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2963 4750 5651 5913 820
Size: 197 926 228 792 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 792 262 228 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 2749 4570 5
Size: 47 2697 1821 2081
Fragments arranged by size:
2697 2081 1821 47
StuI AGG'CCT
Cuts at: 1517 2676 3458 1517
Size: 1159 782 4705
Fragments arranged by size:
4705 1159 782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2195 4085 513
Size: 552 939 191 1890 3074
Fragments arranged by size:
3074 1890 939 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4673 4690 4843 5028 5473 325
Size: 1255 3093 17 153 185 445 1498
Fragments arranged by size:
3093 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2516 3232 3652
Size: 274 80 33 51 1443 539 716 420
Cuts at: 3652 3820 4892 6604 96
Size: 168 1072 1712 138
Fragments arranged by size:
1712 1443 1072 716 539 420 274 168
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6606 6638 6606
Size: 32 6614
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 2288 3982 2288
Size: 1694 4952
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 2657 2786 3038 3122 3290 4775 2657
Size: 129 252 84 168 1485 4528
Fragments arranged by size:
4528 1485 252 168 129 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI