Restriction map for
pFN21AB4270
(Circular) (Six-base) MAPSORT of: pFN21AB4270.seq Check: 4614 from: 1 to: 5962
pFN21AB4270 5962 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2865 4025 5409 5677 1632
Size: 596 637 1160 1384 268 1917
Fragments arranged by size:
1917 1384 1160 637 596 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2385 2933 4861 5838 1203
Size: 1182 548 1928 977 1327
Fragments arranged by size:
1928 1327 1182 977 548
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaI G_GGCC'C
Cuts at: 2989 2989
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2456 3134 3271 3497 3649 5758 1493
Size: 963 678 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 678 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2676 3287 1870
Size: 73 733 611 4545
Fragments arranged by size:
4545 733 611 73
BamHI G'GATC_C
Cuts at: 2829 3292 3659 2829
Size: 463 367 5132
Fragments arranged by size:
5132 463 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2683 2989 3281 1391
Size: 1292 306 292 4072
Fragments arranged by size:
4072 1292 306 292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2002 2962 928
Size: 971 103 960 3928
Fragments arranged by size:
3928 971 960 103
BbvCI CC'TCA_GC
Cuts at: 2858 2858
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3207 3854 5064 3207
Size: 647 1210 4105
Fragments arranged by size:
4105 1210 647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2989 4023 5179 1790
Size: 415 784 1034 1156 2573
Fragments arranged by size:
2573 1156 1034 784 415
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2680 2885 3693
Size: 416 53 83 399 24 1263 205 808
Cuts at: 3693 4651 442
Size: 958 1753
Fragments arranged by size:
1753 1263 958 808 416 399 205 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3142 4622 1239
Size: 1903 1480 2579
Fragments arranged by size:
2579 1903 1480
Bpu10I CC'TnA_GC
Cuts at: 2589 2858 2589
Size: 269 5693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 3074 4087 4952 5250 2845
Size: 229 1013 865 298 3557
Fragments arranged by size:
3557 1013 865 298 229
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2885 2890 4625 882
Size: 617 1386 5 1735 2219
Fragments arranged by size:
2219 1735 1386 617 5
BsaAI yAC'GTr
Cuts at: 493 1204 2936 3753 493
Size: 711 1732 817 2702
Fragments arranged by size:
2702 1732 817 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2083 2113 2143 2173 1178
Size: 30 165 30 680 30 30 30 4967
Fragments arranged by size:
4967 680 165 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5962
BseYI C'CCAG_C
Cuts at: 2982 5165 5661 2982
Size: 2183 496 3283
Fragments arranged by size:
3283 2183 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2683 4023 4108 5179 2683
Size: 1340 85 1071 3466
Fragments arranged by size:
3466 1340 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2683 2989 3281 4023 4108 5179
Size: 399 415 478 306 292 742 85 1071
Cuts at: 5179 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 415 399 306 292
85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2883 3851 5581 2883
Size: 968 1730 3264
Fragments arranged by size:
3264 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 3317 844
Size: 1826 647 3489
Fragments arranged by size:
3489 1826 647
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2129 4457 4631 65
Size: 1183 881 2328 174 1396
Fragments arranged by size:
2328 1396 1183 881 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 2875
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2829 3292 3659 4044 4061 5513 5599 5611
Size: 857 463 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 857 463 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 4321
Fragments arranged by size:
4321 939 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 2403 3478 4272 4292 2025
Size: 378 1075 794 20 3695
Fragments arranged by size:
3695 1075 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2929 3266 2929
Size: 337 5625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3216 4969 809
Size: 507 1900 1753 1802
Fragments arranged by size:
1900 1802 1753 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927
Size: 54 1425 50 275 18 1579 889 1629
Cuts at: 5927 8
Size: 43
Fragments arranged by size:
1629 1579 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 3892 1353
Size: 894 1645 3423
Fragments arranged by size:
3423 1645 894
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2681 2681
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2865 3142 4025 4622 5409 5677
Size: 393 596 637 277 883 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 883 787 637 597 596 393 277
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2861 2861
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2986 2986
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2531 4603 4635 4677
Size: 32 135 32 927 32 2072 32 42
Cuts at: 4677 4709 1373
Size: 32 2626
Fragments arranged by size:
2626 2072 927 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 3306 3552 669
Size: 988 281 87 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2073 5076 5260 1305
Size: 768 3003 184 2007
Fragments arranged by size:
3003 2007 768 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2918 3920 4279 4438 518
Size: 648 915 837 1002 359 159 2042
Fragments arranged by size:
2042 1002 915 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 3109 3378 4055 5203 5448 1606
Size: 592 911 269 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 677 592 269 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 4321
Fragments arranged by size:
4321 939 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2389 2410 3320 4865 1132
Size: 97 1160 21 910 1545 2229
Fragments arranged by size:
2229 1545 1160 910 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 2385 4861 2385
Size: 2476 3486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PmlI CAC'GTG
Cuts at: 2936 2936
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2577 2609 4031 4063 5544 5576 2577
Size: 32 1422 32 1481 32 2963
Fragments arranged by size:
2963 1481 1422 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2124 1613
Size: 511 5451
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2985 2985
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2667 2895 3314 830
Size: 1837 228 419 3478
Fragments arranged by size:
3478 1837 419 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2198 2198
Size: 5962
SacI G_AGCT'C
Cuts at: 2683 2683
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2464 2663 2891 3310 4445 5126 5317
Size: 219 1419 199 228 419 1135 681 191
Cuts at: 5317 826
Size: 1471
Fragments arranged by size:
1471 1419 1135 681 419 228 219 199
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 3089 4066 4967 5229 820
Size: 197 926 881 265 977 901 262 1553
Fragments arranged by size:
1553 977 926 901 881 265 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 2089 3886 5
Size: 47 2037 1797 2081
Fragments arranged by size:
2081 2037 1797 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 3401 513
Size: 552 939 150 1247 3074
Fragments arranged by size:
3074 1247 939 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2192 2781 4208
Size: 274 80 33 51 1443 215 589 1427
Cuts at: 4208 5920 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1427 589 274 215 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 5922 5954 2695
Size: 32 3195 32 2703
Fragments arranged by size:
3195 2703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2693 2693
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2459 4091 2459
Size: 1632 4330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI