Restriction map for
pFN21AB4255
(Circular) (Six-base) MAPSORT of: pFN21AB4255.seq Check: 302 from: 1 to: 5440
pFN21AB4255 5440 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2673 3503 4887 5155 1632
Size: 498 543 830 1384 268 1917
Fragments arranged by size:
1917 1384 830 543 498 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 3183 4755 3183
Size: 1572 3868
ApaI G_GGCC'C
Cuts at: 2333 2333
Size: 5440
ApaLI G'TGCA_C
Cuts at: 2397 3497 4653 2397
Size: 1100 1156 3184
Fragments arranged by size:
3184 1156 1100
ApoI r'AATT_y
Cuts at: 1493 2015 2749 2975 3127 5236 1493
Size: 522 734 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 522 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2441 2770 3137 2441
Size: 329 367 4744
Fragments arranged by size:
4744 367 329
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 2579 2761 4216 618
Size: 325 217 627 677 115 182 1455 1842
Fragments arranged by size:
1842 1455 677 627 325 217 182 115
BanII G_rGCy'C
Cuts at: 1391 2333 2759 1391
Size: 942 426 4072
Fragments arranged by size:
4072 942 426
BbeI G_GCGC'C
Cuts at: 2468 2468
Size: 5440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 928
Size: 971 543 3926
Fragments arranged by size:
3926 971 543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2496 2649 3332 4542 2229
Size: 267 153 683 1210 3127
Fragments arranged by size:
3127 1210 683 267 153
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2555 4051 136
Size: 107 122 71 631 605 883 1496 1525
Fragments arranged by size:
1525 1496 883 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2333 2401 2582 3501 4657 1790
Size: 543 68 181 919 1156 2573
Fragments arranged by size:
2573 1156 919 543 181 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 3171 4129
Size: 416 53 83 399 24 1244 510 958
Cuts at: 4129 442
Size: 1753
Fragments arranged by size:
1753 1244 958 510 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2499 4100 1239
Size: 993 267 1601 2579
Fragments arranged by size:
2579 1601 993 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2082 3565 4430 4728 2082
Size: 1483 865 298 2794
Fragments arranged by size:
2794 1483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2465 3247 3629 4264
Size: 53 83 186 936 932 782 382 635
Cuts at: 4264 275
Size: 1451
Fragments arranged by size:
1451 936 932 782 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2237 2276 3873 4271 4545 4692 5103 2237
Size: 39 1597 398 274 147 411 2574
Fragments arranged by size:
2574 1597 411 398 274 147 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2523 2526 2529 1186
Size: 899 438 3 3 4097
Fragments arranged by size:
4097 899 438 3 3
BseYI C'CCAG_C
Cuts at: 2257 4643 5139 2257
Size: 2386 496 2558
Fragments arranged by size:
2558 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4867
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 2401 2707 3501 3586 4657 2140
Size: 261 306 794 85 1071 2923
Fragments arranged by size:
2923 1071 794 306 261 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2333 2401 2582 2707 2759 3501
Size: 399 350 193 68 181 125 52 742
Cuts at: 3501 3586 4657 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 193 181 125
85 68 52
BspEI T'CCGG_A
Cuts at: 2276 5103 2276
Size: 2827 2613
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 844
Size: 1951 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2531 3327 1394
Size: 429 708 796 3507
Fragments arranged by size:
3507 796 708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2598 2552
Size: 46 5394
BstYI r'GATC_y
Cuts at: 1972 2407 2441 2770 3137 3522 3539 4991 5077
Size: 435 34 329 367 385 17 1452 86
Cuts at: 5077 5089 5435 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 435 385 367 346 329 86
34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2435 2573 513
Size: 552 1370 138 3380
Fragments arranged by size:
3380 1370 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DraIII CAC_nnn'GTG
Cuts at: 2171 2300 2171
Size: 129 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2286 4447 809
Size: 507 970 2161 1802
Fragments arranged by size:
2161 1802 970 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2105 2887
Size: 54 1425 50 275 18 256 19 782
Cuts at: 2887 3776 5405 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 782 275 256 54 50
43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2232 2499 2673 3503 4100 4887
Size: 393 498 102 267 174 830 597 787
Cuts at: 4887 5155 1239
Size: 268 1524
Fragments arranged by size:
1524 830 787 597 498 393 268 267
174 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2097 2097
Size: 5440
EcoO109I rG'GnC_Cy
Cuts at: 2252 2330 2693 2252
Size: 78 363 4999
Fragments arranged by size:
4999 363 78
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 2468 4587 1678
Size: 790 2119 2531
Fragments arranged by size:
2531 2119 790
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KasI G'GCGC_C
Cuts at: 2464 2464
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 4554 4738 1305
Size: 834 2415 184 2007
Fragments arranged by size:
2415 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2088 2107 10
Size: 54 1425 343 256 19 3343
Fragments arranged by size:
3343 1425 343 256 54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 3398 3757 3916 518
Size: 648 2232 359 159 2042
Fragments arranged by size:
2232 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2414 2420 2856 3533 4681 4926 1606
Size: 808 6 436 677 1148 245 2120
Fragments arranged by size:
2120 1148 808 677 436 245 6
NarI GG'CG_CC
Cuts at: 2465 2465
Size: 5440
NcoI C'CATG_G
Cuts at: 513 1065 2435 2573 513
Size: 552 1370 138 3380
Fragments arranged by size:
3380 1370 552 138
NdeI CA'TA_TG
Cuts at: 387 2392 387
Size: 2005 3435
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2552 1348
Size: 1204 4236
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2121 2300 2717 1108
Size: 1013 179 417 3831
Fragments arranged by size:
3831 1013 417 179
PfoI T'CCnGG_A
Cuts at: 2248 2248
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2252 2693 2252
Size: 441 4999
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2329 2329
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2419 2792 830
Size: 1589 373 3478
Fragments arranged by size:
3478 1589 373
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2414 2420 2414
Size: 6 5434
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SexAI A'CCwGG_T
Cuts at: 2296 2296
Size: 5440
SfcI C'TryA_G
Cuts at: 826 1045 2415 2628 2788 3923 4604 4795 826
Size: 219 1370 213 160 1135 681 191 1471
Fragments arranged by size:
1471 1370 1135 681 219 213 191 160
SfoI GGC'GCC
Cuts at: 2466 2466
Size: 5440
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2097 3544 4445 4707 820
Size: 197 926 154 1447 901 262 1553
Fragments arranged by size:
1553 1447 926 901 262 197 154
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2120 2435 2459 2573 2879 513
Size: 552 1055 315 24 114 306 3074
Fragments arranged by size:
3074 1055 552 315 306 114 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2416 3467 3484 3637 3822 4267 325
Size: 1255 836 1051 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1051 836 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5400 5432 5400
Size: 32 5408
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2571 2595 2571
Size: 24 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI