Restriction map for
pFN21AB4249
(Circular) (Six-base) MAPSORT of: pFN21AB4249.seq Check: 9337 from: 1 to: 5791
pFN21AB4249 5791 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2870 2870
Size: 5791
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3854 5238 5506 1632
Size: 941 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 941 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2079 2911 3534 5106 2079
Size: 832 623 1572 2764
Fragments arranged by size:
2764 1572 832 623
ApaLI G'TGCA_C
Cuts at: 2921 3848 5004 2921
Size: 927 1156 3708
Fragments arranged by size:
3708 1156 927
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 3116 1870
Size: 73 663 510 4545
Fragments arranged by size:
4545 663 510 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2349 3024 3110 1391
Size: 958 675 86 4072
Fragments arranged by size:
4072 958 675 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 2686 2776 928
Size: 971 662 125 90 3943
Fragments arranged by size:
3943 971 662 125 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2723 4402 136
Size: 107 122 71 631 605 1051 1679 1525
Fragments arranged by size:
1679 1525 1051 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2925 3852 5008 1790
Size: 1135 927 1156 2573
Fragments arranged by size:
2573 1156 1135 927
BmgBI CAC'GTC
Cuts at: 1318 2565 1318
Size: 1247 4544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3522 4480
Size: 416 53 83 399 24 874 1231 958
Cuts at: 4480 442
Size: 1753
Fragments arranged by size:
1753 1231 958 874 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2891 3028 4451 1239
Size: 1652 137 1423 2579
Fragments arranged by size:
2579 1652 1423 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 2653 4454 882
Size: 617 1077 77 1801 2219
Fragments arranged by size:
2219 1801 1077 617 77
BsaAI yAC'GTr
Cuts at: 493 1204 2352 2412 2776 2812 3582 493
Size: 711 1148 60 364 36 770 2702
Fragments arranged by size:
2702 1148 770 711 364 60 36
BsaBI GATnn'nnATC
Cuts at: 2013 5785 2013
Size: 3772 2019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2726 3598 3980 4615
Size: 53 83 186 936 1193 872 382 635
Cuts at: 4615 275
Size: 1451
Fragments arranged by size:
1451 1193 936 872 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2819 2849 1178
Size: 30 165 30 726 30 660 30 4120
Fragments arranged by size:
4120 726 660 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5791
BseYI C'CCAG_C
Cuts at: 2462 4994 5490 2462
Size: 2532 496 2763
Fragments arranged by size:
2763 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 2828 1163
Size: 573 603 489 4126
Fragments arranged by size:
4126 603 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 2613 2877 2925 3086 3852 3937 5008 2349
Size: 264 264 48 161 766 85 1071 3132
Fragments arranged by size:
3132 1071 766 264 264 161 85 48
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
BsmBI CGTCTCn'nnnn_
Cuts at: 3050 3050
Size: 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2613 2877 2925 3024 3086 3110
Size: 399 559 264 264 48 99 62 24
Cuts at: 3110 3852 3937 5008 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 264 264 99
85 62 48 24
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 3146 844
Size: 2026 276 3489
Fragments arranged by size:
3489 2026 276
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 4286 4460 65
Size: 1183 1017 2021 174 1396
Fragments arranged by size:
2021 1396 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2592 2865 4435 4622 1700
Size: 110 782 273 1570 187 2869
Fragments arranged by size:
2869 1570 782 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 2315 3851 4863 2315
Size: 1536 1012 3243
Fragments arranged by size:
3243 1536 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 794
Size: 1285 4506
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2008 3121 3488 3873 3890 5342 5428 5440
Size: 36 1113 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 2337 513
Size: 552 939 189 144 3967
Fragments arranged by size:
3967 939 552 189 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 2242 2923 3307 4101 4121 2242
Size: 681 384 794 20 3912
Fragments arranged by size:
3912 794 681 384 20
ClaI AT'CG_AT
Cuts at: 1554 3036 1554
Size: 1482 4309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DraIII CAC_nnn'GTG
Cuts at: 2907 2907
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2563 4798 809
Size: 507 1247 2235 1802
Fragments arranged by size:
2235 1802 1247 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2262 2709
Size: 54 1425 50 275 18 230 202 447
Cuts at: 2709 3009 3238 4127 5756 8
Size: 300 229 889 1629 43
Fragments arranged by size:
1629 1425 889 447 300 275 230 229
202 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2393 2888 3721 1353
Size: 671 369 495 833 3423
Fragments arranged by size:
3423 833 671 495 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2347 2347
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 2891 3028 3854 4451 5238 5506
Size: 393 941 318 137 826 597 787 268
Cuts at: 5506 1239
Size: 1524
Fragments arranged by size:
1524 941 826 787 597 393 318 268
137
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 5791
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 4938 1678
Size: 3260 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2819 2851 4432 4464 4506
Size: 32 135 32 1247 32 1581 32 42
Cuts at: 4506 4538 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1247 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 3135 3381 669
Size: 988 281 997 200 246 3079
Fragments arranged by size:
3079 997 988 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 4905 5089 1305
Size: 1293 2307 184 2007
Fragments arranged by size:
2307 2007 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2062 2264 2711 10
Size: 54 1425 343 230 202 447 3090
Fragments arranged by size:
3090 1425 447 343 230 202 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2099 2177 2414 2420 2504 3749
Size: 648 921 12 78 237 6 84 1245
Cuts at: 3749 4108 4267 518
Size: 359 159 2042
Fragments arranged by size:
2042 1245 921 648 359 237 159 84
78 12 6
MspA1I CmG'CkG
Cuts at: 1606 2058 3207 3884 5032 5277 1606
Size: 452 1149 677 1148 245 2120
Fragments arranged by size:
2120 1149 1148 677 452 245
NaeI GCC'GGC
Cuts at: 2867 2867
Size: 5791
NcoI C'CATG_G
Cuts at: 513 1065 2004 2337 513
Size: 552 939 333 3967
Fragments arranged by size:
3967 939 552 333
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NgoMIV G'CCGG_C
Cuts at: 2865 2865
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2116 2900 3149 4694 1132
Size: 97 887 784 249 1545 2229
Fragments arranged by size:
2229 1545 887 784 249 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PmlI CAC'GTG
Cuts at: 2352 2412 2812 2352
Size: 60 400 5331
Fragments arranged by size:
5331 400 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2058 2058
Size: 5791
SacI G_AGCT'C
Cuts at: 2349 2349
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 3005 3065 4039 5751 3005
Size: 60 974 1712 3045
Fragments arranged by size:
3045 1712 974 60
SfcI C'TryA_G
Cuts at: 826 1045 2947 2968 3139 4274 4955 5146 826
Size: 219 1902 21 171 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 219 191 171 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 1517
Size: 1473 4318
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 3072 3230 513
Size: 552 939 333 735 158 3074
Fragments arranged by size:
3074 939 735 552 333 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2293 3818 3835 3988 4173 4618
Size: 1255 614 99 1525 17 153 185 445
Cuts at: 4618 325
Size: 1498
Fragments arranged by size:
1525 1498 1255 614 445 185 153 99
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3003 3063 4037
Size: 274 80 33 51 1443 1026 60 974
Cuts at: 4037 5749 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1026 974 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 2990 3920 2990
Size: 930 4861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI