Restriction map for
pFN21AB4243
(Circular) (Six-base) MAPSORT of: pFN21AB4243.seq Check: 5551 from: 1 to: 9220
pFN21AB4243 9220 bp
With 182 enzymes: SgfI *
March 23, 2015 11:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 2780 2915 2671
Size: 109 135 8976
Fragments arranged by size:
8976 135 109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7030 278
Size: 53 83 186 936 5494 2468
Fragments arranged by size:
5494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3363 6563 1937
Size: 1426 3200 4594
Fragments arranged by size:
4594 3200 1426
Acc65I G'GTAC_C
Cuts at: 6541 6541
Size: 9220
AclI AA'CG_TT
Cuts at: 1563 7347 7720 1563
Size: 5784 373 3063
Fragments arranged by size:
5784 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 4457 7283 8667 8935 1632
Size: 2117 708 2826 1384 268 1917
Fragments arranged by size:
2826 2117 1917 1384 708 268
AfeI AGC'GCT
Cuts at: 2234 6068 2234
Size: 3834 5386
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9023
AflIII A'CryG_T
Cuts at: 1203 2313 3131 5052 5701 5754 8119 9096 1203
Size: 1110 818 1921 649 53 2365 977 1327
Fragments arranged by size:
2365 1921 1327 1110 977 818 649 53
AgeI A'CCGG_T
Cuts at: 8051 8051
Size: 9220
AhdI GACnn_n'nnGTC
Cuts at: 7949 7949
Size: 9220
AleI CACnn'nnGTG
Cuts at: 1166 2774 2909 3797 4968 5442 5639 6315 1166
Size: 1608 135 888 1171 474 197 676 4071
Fragments arranged by size:
4071 1608 1171 888 676 474 197 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5708 5740 5708
Size: 32 9188
AlwNI CAG_nnn'CTG
Cuts at: 2290 3053 3781 5677 6963 8535 2290
Size: 763 728 1896 1286 1572 2975
Fragments arranged by size:
2975 1896 1572 1286 763 728
ApaI G_GGCC'C
Cuts at: 2024 2426 3213 3510 2024
Size: 402 787 297 7734
Fragments arranged by size:
7734 787 402 297
ApaLI G'TGCA_C
Cuts at: 7277 8433 7277
Size: 1156 8064
ApoI r'AATT_y
Cuts at: 1493 6529 6755 6907 9016 1493
Size: 5036 226 152 2109 1697
Fragments arranged by size:
5036 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7774 160
Size: 872 6742 1606
Fragments arranged by size:
6742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9220
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 2981 3037 5720 6159 6545 1870
Size: 73 894 144 56 2683 439 386 4545
Fragments arranged by size:
4545 2683 894 439 386 144 73 56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6473 6506 6473
Size: 33 9187
BamHI G'GATC_C
Cuts at: 2280 2530 6550 6917 2280
Size: 250 4020 367 4583
Fragments arranged by size:
4583 4020 367 250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3785 4198 4333 6541
Size: 325 217 627 221 1777 413 135 2208
Cuts at: 6541 7996 618
Size: 1455 1842
Fragments arranged by size:
2208 1842 1777 1455 627 413 325 221
217 135
BanII G_rGCy'C
Cuts at: 1391 2024 2100 2426 2578 2789 3158 3213 3336
Size: 633 76 326 152 211 369 55 123
Cuts at: 3336 3510 3617 3651 3942 4166 4275 4437 5873
Size: 174 107 34 291 224 109 162 1436
Cuts at: 5873 6539 1391
Size: 666 4072
Fragments arranged by size:
4072 1436 666 633 369 326 291 224
211 174 162 152 123 109 107 76
55 34
BbeI G_GCGC'C
Cuts at: 2012 3789 2012
Size: 1777 7443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3869 4597 5519 5967 6334 928
Size: 971 1970 728 922 448 367 3814
Fragments arranged by size:
3814 1970 971 922 728 448 367
BbvCI CC'TCA_GC
Cuts at: 2276 2291 2276
Size: 15 9205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5701 5735 6053 6087 7411 7445 1903
Size: 34 3764 34 318 34 1324 34 3678
Fragments arranged by size:
3764 3678 1324 318 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7112 8322 7112
Size: 1210 8010
BclI T'GATC_A
Cuts at: 1548 3855 5179 1548
Size: 2307 1324 5589
Fragments arranged by size:
5589 2307 1324
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4044 5463 6294
Size: 107 122 71 631 605 2372 1419 831
Cuts at: 6294 7831 136
Size: 1537 1525
Fragments arranged by size:
2372 1537 1525 1419 831 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3174 9215 3174
Size: 6041 3179
BlpI GC'TnA_GC
Cuts at: 3290 3878 4778 6353 6637 3290
Size: 588 900 1575 284 5873
Fragments arranged by size:
5873 1575 900 588 284
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2426 3213 3510 4338 5358 5646 7281
Size: 234 402 787 297 828 1020 288 1635
Cuts at: 7281 8437 1790
Size: 1156 2573
Fragments arranged by size:
2573 1635 1156 1020 828 787 402 297
288 234
BmgBI CAC'GTC
Cuts at: 1318 5704 5757 1318
Size: 4386 53 4781
Fragments arranged by size:
4781 4386 53
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6951 7909 442
Size: 416 53 83 399 24 5534 958 1753
Fragments arranged by size:
5534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3473 3631 4034 4349 4451 4752 4964 5174
Size: 2234 158 403 315 102 301 212 210
Cuts at: 5174 5281 5633 6386 6506 7880 1239
Size: 107 352 753 120 1374 2579
Fragments arranged by size:
2579 2234 1374 753 403 352 315 301
212 210 158 120 107 102
Bpu10I CC'TnA_GC
Cuts at: 2276 2291 3152 2276
Size: 15 861 8344
Fragments arranged by size:
8344 861 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3974 4814 4832 5513 7345 8210 8508 3974
Size: 840 18 681 1832 865 298 4686
Fragments arranged by size:
4686 1832 865 840 681 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3455 3584 5078 5201 5945 7883 882
Size: 617 1956 129 1494 123 744 1938 2219
Fragments arranged by size:
2219 1956 1938 1494 744 617 129 123
BsaAI yAC'GTr
Cuts at: 493 1204 2677 2803 3030 3715 5029 5536 7011
Size: 711 1473 126 227 685 1314 507 1475
Cuts at: 7011 493
Size: 2702
Fragments arranged by size:
2702 1475 1473 1314 711 685 507 227
126
BsaBI GATnn'nnATC
Cuts at: 9214 9214
Size: 9220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2931 3786 5044
Size: 53 83 186 936 476 922 855 1258
Cuts at: 5044 5484 6339 7027 7409 8044 275
Size: 440 855 688 382 635 1451
Fragments arranged by size:
1451 1258 936 922 855 855 688 635
476 440 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3379 7653 8051 8325 8472 8883 3379
Size: 4274 398 274 147 411 3716
Fragments arranged by size:
4274 3716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4853 4883 5108 5138 5147
Size: 30 165 30 3450 30 225 30 9
Cuts at: 5147 5177 5570 5600 1178
Size: 30 393 30 4798
Fragments arranged by size:
4798 3450 393 225 165 30 30 30
30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2647 3335 3641 4577 5171 1186
Size: 1461 688 306 936 594 5235
Fragments arranged by size:
5235 1461 936 688 594 306
BseYI C'CCAG_C
Cuts at: 3215 3882 4100 4240 4542 5553 6426 8423 8919
Size: 667 218 140 302 1011 873 1997 496
Cuts at: 8919 3215
Size: 3516
Fragments arranged by size:
3516 1997 1011 873 667 496 302 218
140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 2283 2493 3332 5267 5591 6464
Size: 573 370 177 210 839 1935 324 873
Cuts at: 6464 1163
Size: 3919
Fragments arranged by size:
3919 1935 873 839 573 370 324 210
177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3189 5126 6000 6063 6434 6670
Size: 275 186 1188 1937 874 63 371 236
Cuts at: 6670 7431 8459 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1937 1188 1028 874 761 371 275
236 186 63
BsiHKAI G_wGCw'C
Cuts at: 3387 3483 3651 4275 4818 5834 7281 7366 8437
Size: 96 168 624 543 1016 1447 85 1071
Cuts at: 8437 3387
Size: 4170
Fragments arranged by size:
4170 1447 1071 1016 624 543 168 96
85
BsmI GAATG_Cn'
Cuts at: 6731 6824 6731
Size: 93 9127
BsmBI CGTCTCn'nnnn_
Cuts at: 2234 4741 2234
Size: 2507 6713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2100 2426 2578 2789 3158 3213
Size: 399 234 76 326 152 211 369 55
Cuts at: 3213 3336 3387 3483 3510 3617 3651 3942 4166
Size: 123 51 96 27 107 34 291 224
Cuts at: 4166 4275 4338 4437 4818 5358 5646 5834 5873
Size: 109 63 99 381 540 288 188 39
Cuts at: 5873 6539 7281 7366 8437 1391
Size: 666 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 540 399 381 369
326 291 288 234 224 211 188 152
123 109 107 99 96 85 76 63
55 51 39 34 27
BspEI T'CCGG_A
Cuts at: 8883 8883
Size: 9220
BspHI T'CATG_A
Cuts at: 3858 5139 5544 7109 8839 3858
Size: 1281 405 1565 1730 4239
Fragments arranged by size:
4239 1730 1565 1281 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 2780 2915 3576 4092 5411 6575 844
Size: 1827 109 135 661 516 1319 1164 3489
Fragments arranged by size:
3489 1827 1319 1164 661 516 135 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 3768 4825 5497 6370 7107 1394
Size: 429 623 1322 1057 672 873 737 3507
Fragments arranged by size:
3507 1322 1057 873 737 672 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 4623 5058 7715 7889 65
Size: 1183 1303 2072 435 2657 174 1396
Fragments arranged by size:
2657 2072 1396 1303 1183 435 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2642 3325 5040 5420 5447 6058 6320
Size: 110 832 683 1715 380 27 611 262
Cuts at: 6320 6433 7864 8051 1700
Size: 113 1431 187 2869
Fragments arranged by size:
2869 1715 1431 832 683 611 380 262
187 113 110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9220
BssHII G'CGCG_C
Cuts at: 1925 3261 1925
Size: 1336 7884
BssSI C'ACGA_G
Cuts at: 2190 2738 4215 5600 7280 8292 2190
Size: 548 1477 1385 1680 1012 3118
Fragments arranged by size:
3118 1680 1477 1385 1012 548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3547 4689 5466 794
Size: 2753 1142 777 4548
Fragments arranged by size:
4548 2753 1142 777
BstBI TT'CG_AA
Cuts at: 8024 8024
Size: 9220
BstEII G'GTnAC_C
Cuts at: 1184 2177 3229 4060 1184
Size: 993 1052 831 6344
Fragments arranged by size:
6344 1052 993 831
BstXI CCAn_nnnn'nTGG
Cuts at: 3935 5029 5251 3935
Size: 1094 222 7904
Fragments arranged by size:
7904 1094 222
BstYI r'GATC_y
Cuts at: 1972 2280 2530 3049 3174 3924 4261 6097 6550
Size: 308 250 519 125 750 337 1836 453
Cuts at: 6550 6917 7302 7319 8771 8857 8869 9215 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1836 1452 750 519 453 385 367
346 337 308 250 125 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2172 2300 2781 2853 2991
Size: 552 939 45 123 128 481 72 138
Cuts at: 2991 3225 3266 3349 3372 3390 3438 3620 3670
Size: 234 41 83 23 18 48 182 50
Cuts at: 3670 3798 4599 5347 5619 5955 6438 513
Size: 128 801 748 272 336 483 3295
Fragments arranged by size:
3295 939 801 748 552 483 481 336
272 234 182 138 128 128 123 83
72 50 48 45 41 23 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4783 4936 5911 487
Size: 683 56 3557 153 975 3796
Fragments arranged by size:
3796 3557 975 683 153 56
BtsI GCAGTG_nn'
Cuts at: 2277 2599 3320 4202 5011 5437 5449 6310 6322
Size: 322 721 882 809 426 12 861 12
Cuts at: 6322 6736 7530 7550 2277
Size: 414 794 20 3947
Fragments arranged by size:
3947 882 861 809 794 721 426 414
322 20 12 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3289 3324 4198 4233 529
Size: 35 2725 35 874 35 5516
Fragments arranged by size:
5516 2725 874 35 35 35
DraI TTT'AAA
Cuts at: 6524 6524
Size: 9220
DraIII CAC_nnn'GTG
Cuts at: 3391 3415 3391
Size: 24 9196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8227 809
Size: 507 6911 1802
Fragments arranged by size:
6911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 2778 2895
Size: 54 1425 50 275 18 798 150 117
Cuts at: 2895 2913 3186 3202 3255 3673 3958 4156 4438
Size: 18 273 16 53 418 285 198 282
Cuts at: 4438 4596 5253 5339 5530 6056 6060 6166 6206
Size: 158 657 86 191 526 4 106 40
Cuts at: 6206 6256 6286 6431 6435 6667 7556 9185 8
Size: 50 30 145 4 232 889 1629 43
Fragments arranged by size:
1629 1425 889 798 657 526 418 285
282 275 273 232 198 191 158 150
145 117 106 86 54 53 50 50
43 40 30 18 18 16 4 4
EagI C'GGCC_G
Cuts at: 1537 1812 3186 6060 6431 6667 1537
Size: 275 1374 2874 371 236 4090
Fragments arranged by size:
4090 2874 1374 371 275 236
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 3659 4263 4460 4472 7150 1353
Size: 735 1571 604 197 12 2678 3423
Fragments arranged by size:
3423 2678 1571 735 604 197 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2687 3743 4826 5306 5963 7807
Size: 1064 138 1098 1056 1083 480 657 1844
Cuts at: 7807 8181 8327 387
Size: 374 146 1280
Fragments arranged by size:
1844 1280 1098 1083 1064 1056 657 480
374 146 138
EcoICRI GAG'CTC
Cuts at: 3649 4273 3649
Size: 624 8596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3473 3631 3749 4034 4349 4451 4457
Size: 393 1841 158 118 285 315 102 6
Cuts at: 4457 4752 4964 5174 5281 5633 6386 6506 7283
Size: 295 212 210 107 352 753 120 777
Cuts at: 7283 7880 8667 8935 1239
Size: 597 787 268 1524
Fragments arranged by size:
1841 1524 787 777 753 597 393 352
315 295 285 268 212 210 158 120
118 107 102 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 3452 5612 2381
Size: 1071 2160 5989
Fragments arranged by size:
5989 2160 1071
EcoO109I rG'GnC_Cy
Cuts at: 2020 2021 2827 3209 3210 3250 3507 3690 3724
Size: 1 806 382 1 40 257 183 34
Cuts at: 3724 3778 4426 4732 5550 5605 2020
Size: 54 648 306 818 55 5635
Fragments arranged by size:
5635 818 806 648 382 306 257 183
55 54 40 34 1 1
EcoRI G'AATT_C
Cuts at: 6529 6907 6529
Size: 378 8842
FseI GG_CCGG'CC
Cuts at: 6062 6437 6062
Size: 375 8845
FspI TGC'GCA
Cuts at: 4208 7726 4208
Size: 3518 5702
HaeII r_GCGC'y
Cuts at: 1678 2012 2236 2643 3789 6070 8367 1678
Size: 334 224 407 1146 2281 2297 2531
Fragments arranged by size:
2531 2297 2281 1146 407 334 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 3569 3601 4853
Size: 32 135 32 1469 32 496 32 1252
Cuts at: 4853 4885 5108 5140 5147 5179 5263 5295 6155
Size: 32 223 32 7 32 84 32 860
Cuts at: 6155 6187 7861 7893 7935 7967 1373
Size: 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 1469 1252 860 496 223 135
84 42 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3193 6564 6810 669
Size: 988 281 286 969 3371 246 3079
Fragments arranged by size:
3371 3079 988 969 286 281 246
HindIII A'AGCT_T
Cuts at: 748 2366 6499 748
Size: 1618 4133 3469
Fragments arranged by size:
4133 3469 1618
HpaI GTT'AAC
Cuts at: 6810 6810
Size: 9220
KasI G'GCGC_C
Cuts at: 2008 3785 2008
Size: 1777 7443
KpnI G_GTAC'C
Cuts at: 6545 6545
Size: 9220
MfeI C'AATT_G
Cuts at: 6819 6819
Size: 9220
MluI A'CGCG_T
Cuts at: 5052 9096 5052
Size: 4044 5176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2056 2840 3567 4932 8334 8518 1305
Size: 751 784 727 1365 3402 184 2007
Fragments arranged by size:
3402 2007 1365 784 751 727 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 2915 5255 5532 6168
Size: 54 1425 343 798 285 2340 277 636
Cuts at: 6168 6258 6288 10
Size: 90 30 2942
Fragments arranged by size:
2942 2340 1425 798 636 343 285 277
90 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2774 2852 2909 3308 3797 4139
Size: 648 1251 357 78 57 399 489 342
Cuts at: 4139 4193 4446 4968 5138 5289 5442 5639 5711
Size: 54 253 522 170 151 153 197 72
Cuts at: 5711 6315 7178 7537 7696 518
Size: 604 863 359 159 2042
Fragments arranged by size:
2042 1251 863 648 604 522 489 399
359 357 342 253 197 170 159 153
151 78 72 57 54
MspA1I CmG'CkG
Cuts at: 1606 2290 2302 2392 2481 2563 3268 3351 3622
Size: 684 12 90 89 82 705 83 271
Cuts at: 3622 3882 4240 4327 4525 5077 5122 5944 6430
Size: 260 358 87 198 552 45 822 486
Cuts at: 6430 6636 7313 8461 8706 1606
Size: 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 705 684 677 552 486
358 271 260 245 206 198 90 89
87 83 82 45 12
NaeI GCC'GGC
Cuts at: 2644 5422 6060 6322 6435 2644
Size: 2778 638 262 113 5429
Fragments arranged by size:
5429 2778 638 262 113
NarI GG'CG_CC
Cuts at: 2009 3786 2009
Size: 1777 7443
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 2991 3372 3670 5347 6438
Size: 552 939 45 942 381 298 1677 1091
Cuts at: 6438 513
Size: 3295
Fragments arranged by size:
3295 1677 1091 942 939 552 381 298
45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9220
NgoMIV G'CCGG_C
Cuts at: 2642 5420 6058 6320 6433 2642
Size: 2778 638 262 113 5429
Fragments arranged by size:
5429 2778 638 262 113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9220
NotI GC'GGCC_GC
Cuts at: 6667 6667
Size: 9220
NruI TCG'CGA
Cuts at: 5930 5930
Size: 9220
NspI r_CATG'y
Cuts at: 1132 1229 2851 4777 5977 6121 6578 8123 1132
Size: 97 1622 1926 1200 144 457 1545 2229
Fragments arranged by size:
2229 1926 1622 1545 1200 457 144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9220
PciI A'CATG_T
Cuts at: 8119 8119
Size: 9220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3030 5677 1108
Size: 1922 2647 4651
Fragments arranged by size:
4651 2647 1922
PfoI T'CCnGG_A
Cuts at: 3608 3657 4278 6173 3608
Size: 49 621 1895 6655
Fragments arranged by size:
6655 1895 621 49
PmeI GTTT'AAAC
Cuts at: 6524 6524
Size: 9220
PmlI CAC'GTG
Cuts at: 3030 5536 3030
Size: 2506 6714
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6449 6481 7289 7321 8802 8834 6449
Size: 32 808 32 1481 32 6835
Fragments arranged by size:
6835 1481 808 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 3690 3724 4732 5550 2827
Size: 863 34 1008 818 6497
Fragments arranged by size:
6497 1008 863 818 34
PshAI GACnn'nnGTC
Cuts at: 1613 5135 6338 1613
Size: 3522 1203 4495
Fragments arranged by size:
4495 3522 1203
PsiI TTA'TAA
Cuts at: 6790 6790
Size: 9220
PspOMI G'GGCC_C
Cuts at: 2020 2422 3209 3506 2020
Size: 402 787 297 7734
Fragments arranged by size:
7734 787 402 297
PspXI vC'TCGA_Gb
Cuts at: 5720 5720
Size: 9220
PstI C_TGCA'G
Cuts at: 830 2583 3822 5444 6317 6572 830
Size: 1753 1239 1622 873 255 3478
Fragments arranged by size:
3478 1753 1622 1239 873 255
PvuI CG_AT'CG
Cuts at: 2001 6000 2001
Size: 3999 5221
PvuII CAG'CTG
Cuts at: 2392 2563 3882 4327 4525 2392
Size: 171 1319 445 198 7087
Fragments arranged by size:
7087 1319 445 198 171
SacI G_AGCT'C
Cuts at: 3651 4275 3651
Size: 624 8596
SacII CC_GC'GG
Cuts at: 2303 3269 3352 3623 2303
Size: 966 83 271 7900
Fragments arranged by size:
7900 966 271 83
SalI G'TCGA_C
Cuts at: 1936 6562 1936
Size: 4626 4594
SapI GCTCTTCn'nnn_
Cuts at: 2088 4263 4472 2088
Size: 2175 209 6836
Fragments arranged by size:
6836 2175 209
SbfI CC_TGCA'GG
Cuts at: 6572 6572
Size: 9220
ScaI AGT'ACT
Cuts at: 6053 6086 7468 9180 6053
Size: 33 1382 1712 6093
Fragments arranged by size:
6093 1712 1382 33
SexAI A'CCwGG_T
Cuts at: 2504 2504
Size: 9220
SfcI C'TryA_G
Cuts at: 826 1045 2579 3818 4295 5440 5905 6313 6568
Size: 219 1534 1239 477 1145 465 408 255
Cuts at: 6568 7703 8384 8575 826
Size: 1135 681 191 1471
Fragments arranged by size:
1534 1471 1239 1145 1135 681 477 465
408 255 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 6294 6294
Size: 9220
SfoI GGC'GCC
Cuts at: 2010 3787 2010
Size: 1777 7443
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9220
SgrAI Cr'CCGG_yG
Cuts at: 1700 2642 1700
Size: 942 8278
SmaI CCC'GGG
Cuts at: 1872 2983 6161 6547 1872
Size: 1111 3178 386 4545
Fragments arranged by size:
4545 3178 1111 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3989 4829 4847 5492 5720 7324
Size: 197 926 2046 840 18 645 228 1604
Cuts at: 7324 8225 8487 820
Size: 901 262 1553
Fragments arranged by size:
2046 1604 1553 926 901 840 645 262
228 197 18
SnaBI TAC'GTA
Cuts at: 493 7011 493
Size: 6518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9220
SphI G_CATG'C
Cuts at: 1132 4777 5977 6578 1132
Size: 3645 1200 601 3774
Fragments arranged by size:
3774 3645 1200 601
SspI AAT'ATT
Cuts at: 5 52 7144 5
Size: 47 7092 2081
Fragments arranged by size:
7092 2081 47
StuI AGG'CCT
Cuts at: 1517 5921 1517
Size: 4404 4816
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2121 2991 3372 3670 4728
Size: 552 939 45 72 870 381 298 1058
Cuts at: 4728 5347 5745 6438 6659 513
Size: 619 398 693 221 3074
Fragments arranged by size:
3074 1058 939 870 693 619 552 398
381 298 221 72 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3169 3925 4390 4567 7247 7264 7417
Size: 1255 1589 756 465 177 2680 17 153
Cuts at: 7417 7602 8047 325
Size: 185 445 1498
Fragments arranged by size:
2680 1589 1498 1255 756 465 445 185
177 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3930 5586 6051
Size: 274 80 33 51 1443 1953 1656 465
Cuts at: 6051 6084 7466 9178 96
Size: 33 1382 1712 138
Fragments arranged by size:
1953 1712 1656 1443 1382 465 274 138
80 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4365 4369 4397 4401 5161 5193 9180 9212 4365
Size: 4 28 4 760 32 3987 32 4373
Fragments arranged by size:
4373 3987 760 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9220
XbaI T'CTAG_A
Cuts at: 6556 6556
Size: 9220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2196 2339 2396 2416 2805 2923 3223 4403 4735
Size: 143 57 20 389 118 300 1180 332
Cuts at: 4735 4804 5690 5860 2196
Size: 69 886 170 5556
Fragments arranged by size:
5556 1180 886 389 332 300 170 143
118 69 57 20
XhoI C'TCGA_G
Cuts at: 1943 5720 1943
Size: 3777 5443
XmaI C'CCGG_G
Cuts at: 1870 2981 6159 6545 1870
Size: 1111 3178 386 4545
Fragments arranged by size:
4545 3178 1111 386
XmnI GAAnn'nnTTC
Cuts at: 7349 7349
Size: 9220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7028 276
Size: 53 83 186 936 5494 2468
Fragments arranged by size:
5494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV
FalI FspAI NsiI PacI PsrI RsrII SanDI SrfI
SwaI