Restriction map for
pFN21AB4240
(Circular) (Six-base) MAPSORT of: pFN21AB4240.seq Check: 6308 from: 1 to: 6118
pFN21AB4240 6118 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2434 2453 2434
Size: 19 6099
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 3461 1937
Size: 792 732 4594
Fragments arranged by size:
4594 792 732
Acc65I G'GTAC_C
Cuts at: 2702 3439 2702
Size: 737 5381
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2406 2634 2966 3089 3211 4181 5565 5833
Size: 774 228 332 123 122 970 1384 268
Cuts at: 5833 1632
Size: 1917
Fragments arranged by size:
1917 1384 970 774 332 268 228 123
122
AfeI AGC'GCT
Cuts at: 2549 2549
Size: 6118
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 3100 5017 5994 1203
Size: 1897 1917 977 1327
Fragments arranged by size:
1917 1897 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3365 1184
Size: 2181 3937
AlwNI CAG_nnn'CTG
Cuts at: 3861 5433 3861
Size: 1572 4546
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2617 3427 3653 3805 5914 1493
Size: 1124 810 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 810 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2171 2198 2702 2747
Size: 325 217 627 248 136 27 504 45
Cuts at: 2747 2848 3439 4894 618
Size: 101 591 1455 1842
Fragments arranged by size:
1842 1455 627 591 504 325 248 217
136 101 45 27
BanII G_rGCy'C
Cuts at: 1391 2540 3437 1391
Size: 1149 897 4072
Fragments arranged by size:
4072 1149 897
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BbvCI CC'TCA_GC
Cuts at: 2050 2245 2304 3363 3381 2050
Size: 195 59 1059 18 4787
Fragments arranged by size:
4787 1059 195 59 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3377 4729 136
Size: 107 122 71 631 605 1705 1352 1525
Fragments arranged by size:
1705 1525 1352 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3405 3535 3405
Size: 130 5988
Bme1580I G_kGCm'C
Cuts at: 1790 2201 4179 5335 1790
Size: 411 1978 1156 2573
Fragments arranged by size:
2573 1978 1156 411
BmgBI CAC'GTC
Cuts at: 1318 3101 1318
Size: 1783 4335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 3849 4807
Size: 416 53 83 399 24 1064 1368 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1753 1368 1064 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3207 4778 1239
Size: 1968 1571 2579
Fragments arranged by size:
2579 1968 1571
Bpu10I CC'TnA_GC
Cuts at: 2050 2245 2304 3038 3363 3381 2050
Size: 195 59 734 325 18 4787
Fragments arranged by size:
4787 734 325 195 59 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4243 5108 5406 4243
Size: 865 298 4955
Fragments arranged by size:
4955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2879 3909 493
Size: 711 1675 1030 2702
Fragments arranged by size:
2702 1675 1030 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2574 2874 3319 3351 1186
Size: 1096 292 300 445 32 3953
Fragments arranged by size:
3953 1096 445 300 292 32
BseYI C'CCAG_C
Cuts at: 5321 5817 5321
Size: 496 5622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 3381 1163
Size: 573 322 1323 3900
Fragments arranged by size:
3900 1323 573 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4179 4264 5335 4179
Size: 85 1071 4962
Fragments arranged by size:
4962 1071 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2540 3437 4179 4264 5335 1391
Size: 399 411 339 897 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 411 399 339 85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 2453 2998 3357 3473 844
Size: 1590 19 545 359 116 3489
Fragments arranged by size:
3489 1590 545 359 116 19
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3328 4613 4787 65
Size: 1183 2080 1285 174 1396
Fragments arranged by size:
2080 1396 1285 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3290 3448 3815 4200 4217 5669 5755 5767
Size: 1318 158 367 385 17 1452 86 12
Cuts at: 5767 6113 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1318 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3256 513
Size: 552 939 1252 3375
Fragments arranged by size:
3375 1252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2298 2933 3390 3634 4428 4448 2298
Size: 635 457 244 794 20 3968
Fragments arranged by size:
3968 794 635 457 244 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 2497 3422 2497
Size: 925 5193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2668 5125 809
Size: 507 1352 2457 1802
Fragments arranged by size:
2457 1802 1352 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3565 4454 6083
Size: 54 1425 50 275 18 1735 889 1629
Cuts at: 6083 8
Size: 43
Fragments arranged by size:
1735 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2557 2650 2690 4048 1353
Size: 807 397 93 40 1358 3423
Fragments arranged by size:
3423 1358 807 397 93 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2124 2674 3068 3111 4705 5079
Size: 1064 138 535 550 394 43 1594 374
Cuts at: 5079 5225 387
Size: 146 1280
Fragments arranged by size:
1594 1280 1064 550 535 394 374 146
138 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 2634 2966 3089 3207 3211 4181
Size: 393 774 228 332 123 118 4 970
Cuts at: 4181 4778 5565 5833 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 970 787 774 597 393 332 268
228 123 118 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2589 2589
Size: 6118
EcoO109I rG'GnC_Cy
Cuts at: 2808 2808
Size: 6118
EcoRI G'AATT_C
Cuts at: 2617 3427 3805 2617
Size: 810 378 4930
Fragments arranged by size:
4930 810 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2781 3241 3430 2781
Size: 460 189 5469
Fragments arranged by size:
5469 460 189
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2139 2551 5265 1678
Size: 461 412 2714 2531
Fragments arranged by size:
2714 2531 461 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2174 2206 2258
Size: 32 135 32 514 32 56 32 52
Cuts at: 2258 2290 2353 2385 2501 2533 4759 4791 4833
Size: 32 63 32 116 32 2226 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 2226 514 135 116 63 56 52
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 2706 3443 2706
Size: 737 5381
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2846 3055 4076 4435 4594 518
Size: 648 1680 209 1021 359 159 2042
Fragments arranged by size:
2042 1680 1021 648 359 209 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2384 3534 4211 5359 5604 1606
Size: 643 135 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 643 245 135
NcoI C'CATG_G
Cuts at: 513 1065 2004 3256 513
Size: 552 939 1252 3375
Fragments arranged by size:
3375 1252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3239 3476 5021 1132
Size: 97 2010 237 1545 2229
Fragments arranged by size:
2229 2010 1545 237 97
PasI CC'CwG_GG
Cuts at: 1348 2723 1348
Size: 1375 4743
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PfoI T'CCnGG_A
Cuts at: 2785 2785
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2808 2808
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2368 3470 830
Size: 1538 1102 3478
Fragments arranged by size:
3478 1538 1102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2249 2384 2249
Size: 135 5983
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SapI GCTCTTCn'nnn_
Cuts at: 2557 2557
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SexAI A'CCwGG_T
Cuts at: 2666 2666
Size: 6118
SfcI C'TryA_G
Cuts at: 826 1045 2310 2364 3466 4601 5282 5473 826
Size: 219 1265 54 1102 1135 681 191 1471
Fragments arranged by size:
1471 1265 1135 1102 681 219 191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4222 5123 5385 820
Size: 197 926 2279 901 262 1553
Fragments arranged by size:
2279 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2879 3909 493
Size: 2386 1030 2702
Fragments arranged by size:
2702 2386 1030
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3239 3476 1132
Size: 2107 237 3774
Fragments arranged by size:
3774 2107 237
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2004 3256 3557 513
Size: 552 939 1252 301 3074
Fragments arranged by size:
3074 1252 939 552 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2728 4145 4162 4315 4500 4945 325
Size: 1255 1148 1417 17 153 185 445 1498
Fragments arranged by size:
1498 1417 1255 1148 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4364 6076 96
Size: 274 80 33 51 1443 2387 1712 138
Fragments arranged by size:
2387 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 2513 1316
Size: 1197 4921
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3089 3152 3089
Size: 63 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 2621 4247 2621
Size: 1626 4492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI