Restriction map for
pFN21AB4237
(Circular) (Six-base) MAPSORT of: pFN21AB4237.seq Check: 5333 from: 1 to: 7156
pFN21AB4237 7156 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 2432 2350
Size: 82 7074
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4966 278
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4499 1937
Size: 2562 4594
Acc65I G'GTAC_C
Cuts at: 4477 4477
Size: 7156
AclI AA'CG_TT
Cuts at: 1563 5283 5656 1563
Size: 3720 373 3063
Fragments arranged by size:
3720 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2416 2633 2918 3426 5219 6603 6871 1632
Size: 784 217 285 508 1793 1384 268 1917
Fragments arranged by size:
1917 1793 1384 784 508 285 268 217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6959
AflIII A'CryG_T
Cuts at: 1203 2574 2961 6055 7032 1203
Size: 1371 387 3094 977 1327
Fragments arranged by size:
3094 1371 1327 977 387
AgeI A'CCGG_T
Cuts at: 5987 5987
Size: 7156
AhdI GACnn_n'nnGTC
Cuts at: 5885 5885
Size: 7156
AleI CACnn'nnGTG
Cuts at: 1166 3386 1166
Size: 2220 4936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 7124
AlwNI CAG_nnn'CTG
Cuts at: 2627 4899 6471 2627
Size: 2272 1572 3312
Fragments arranged by size:
3312 2272 1572
ApaI G_GGCC'C
Cuts at: 2703 3529 3908 2703
Size: 826 379 5951
Fragments arranged by size:
5951 826 379
ApaLI G'TGCA_C
Cuts at: 2329 5213 6369 2329
Size: 2884 1156 3116
Fragments arranged by size:
3116 2884 1156
ApoI r'AATT_y
Cuts at: 1493 3873 3944 3956 4331 4465 4691 4843 6952
Size: 2380 71 12 375 134 226 152 2109
Cuts at: 6952 1493
Size: 1697
Fragments arranged by size:
2380 2109 1697 375 226 152 134 71
12
AseI AT'TA_AT
Cuts at: 160 1032 3612 5710 160
Size: 872 2580 2098 1606
Fragments arranged by size:
2580 2098 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2275 2666 3044 3549 3784 4038
Size: 73 135 197 391 378 505 235 254
Cuts at: 4038 4481 1870
Size: 443 4545
Fragments arranged by size:
4545 505 443 391 378 254 235 197
135 73
BamHI G'GATC_C
Cuts at: 3962 4486 4853 3962
Size: 524 367 6265
Fragments arranged by size:
6265 524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2141 2257 4477 5932
Size: 325 217 627 286 68 116 2220 1455
Cuts at: 5932 618
Size: 1842
Fragments arranged by size:
2220 1842 1455 627 325 286 217 116
68
BanII G_rGCy'C
Cuts at: 1391 2703 3476 3529 3579 3724 3908 4475 1391
Size: 1312 773 53 50 145 184 567 4072
Fragments arranged by size:
4072 1312 773 567 184 145 53 50
BbeI G_GCGC'C
Cuts at: 2077 2145 2077
Size: 68 7088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6185
BbvCI CC'TCA_GC
Cuts at: 3132 3132
Size: 7156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5347 5381 1903
Size: 34 50 34 3326 34 3678
Fragments arranged by size:
3678 3326 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5048 6258 5048
Size: 1210 5946
BclI T'GATC_A
Cuts at: 1548 3894 1548
Size: 2346 4810
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5767 136
Size: 107 122 71 631 605 4095 1525
Fragments arranged by size:
4095 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4299 7151 4299
Size: 2852 4304
BlpI GC'TnA_GC
Cuts at: 3578 4573 3578
Size: 995 6161
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2333 2703 3529 3908 4425 5217 6373
Size: 246 297 370 826 379 517 792 1156
Cuts at: 6373 1790
Size: 2573
Fragments arranged by size:
2573 1156 826 792 517 379 370 297
246
BmgBI CAC'GTC
Cuts at: 1318 2575 3995 1318
Size: 1257 1420 4479
Fragments arranged by size:
4479 1420 1257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3388 3684 4887
Size: 416 53 83 399 24 1971 296 1203
Cuts at: 4887 5845 442
Size: 958 1753
Fragments arranged by size:
1971 1753 1203 958 416 399 296 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4343 5816 1239
Size: 3104 1473 2579
Fragments arranged by size:
3104 2579 1473
Bpu10I CC'TnA_GC
Cuts at: 2265 3132 2265
Size: 867 6289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 5281 6146 6444 2426
Size: 2855 865 298 3138
Fragments arranged by size:
3138 2855 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2663 3285 5819 882
Size: 617 1164 622 2534 2219
Fragments arranged by size:
2534 2219 1164 622 617
BsaAI yAC'GTr
Cuts at: 493 1204 4947 493
Size: 711 3743 2702
Fragments arranged by size:
3743 2702 711
BsaBI GATnn'nnATC
Cuts at: 3540 7150 3540
Size: 3610 3546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 2142 2815 4963
Size: 53 83 186 936 541 68 673 2148
Cuts at: 4963 5345 5980 275
Size: 382 635 1451
Fragments arranged by size:
2148 1451 936 673 635 541 382 186
83 68 53
BsaWI w'CCGG_w
Cuts at: 3294 5589 5987 6261 6408 6819 3294
Size: 2295 398 274 147 411 3631
Fragments arranged by size:
3631 2295 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 3923 3953 1178
Size: 30 165 30 1761 30 729 30 4381
Fragments arranged by size:
4381 1761 729 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3069 3072 3149 3225 1186
Size: 1883 3 77 76 5117
Fragments arranged by size:
5117 1883 77 76 3
BseYI C'CCAG_C
Cuts at: 2312 2533 2726 3259 6359 6855 2312
Size: 221 193 533 3100 496 2613
Fragments arranged by size:
3100 2613 533 496 221 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3155 1163
Size: 573 1419 5164
Fragments arranged by size:
5164 1419 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2242 4606 5367 6395 1540
Size: 275 186 241 2364 761 1028 2301
Fragments arranged by size:
2364 2301 1028 761 275 241 186
BsiHKAI G_wGCw'C
Cuts at: 2288 2333 3476 3724 5217 5302 6373 2288
Size: 45 1143 248 1493 85 1071 3071
Fragments arranged by size:
3071 1493 1143 1071 248 85 45
BsmI GAATG_Cn'
Cuts at: 3334 4667 4760 3334
Size: 1333 93 5730
Fragments arranged by size:
5730 1333 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2288 2333 2703 3476 3529 3579
Size: 399 246 252 45 370 773 53 50
Cuts at: 3579 3724 3908 4425 4475 5217 5302 6373 1391
Size: 145 184 517 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 773 742 517 399 370 252
246 184 145 85 53 50 50 45
BspEI T'CCGG_A
Cuts at: 6819 6819
Size: 7156
BspHI T'CATG_A
Cuts at: 3337 5045 6775 3337
Size: 1708 1730 3718
Fragments arranged by size:
3718 1730 1708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 2432 2697 4511 844
Size: 1506 82 265 1814 3489
Fragments arranged by size:
3489 1814 1506 265 82
BsrBI CCG'CTC
Cuts at: 1394 1823 5043 1394
Size: 429 3220 3507
Fragments arranged by size:
3507 3220 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 4186 4218 5651 5825 65
Size: 1183 1473 1465 32 1433 174 1396
Fragments arranged by size:
1473 1465 1433 1396 1183 174 32
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 2254 2832 3368 5800 5987 1700
Size: 110 427 17 578 536 2432 187 2869
Fragments arranged by size:
2869 2432 578 536 427 187 110 17
BsrGI T'GTAC_A
Cuts at: 96 4357 96
Size: 4261 2895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7156
BssSI C'ACGA_G
Cuts at: 2876 5216 6228 2876
Size: 2340 1012 3804
Fragments arranged by size:
3804 2340 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2179 4158 794
Size: 1385 1979 3792
Fragments arranged by size:
3792 1979 1385
BstBI TT'CG_AA
Cuts at: 5960 5960
Size: 7156
BstEII G'GTnAC_C
Cuts at: 1184 2050 3649 1184
Size: 866 1599 4691
Fragments arranged by size:
4691 1599 866
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 3190 4321 2710
Size: 480 1131 5545
Fragments arranged by size:
5545 1131 480
BstYI r'GATC_y
Cuts at: 1972 3297 3962 4299 4486 4853 5238 5255 6707
Size: 1325 665 337 187 367 385 17 1452
Cuts at: 6707 6793 6805 7151 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1325 665 385 367 346 337
187 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3084 3084
Size: 7156
BtgI C'CryG_G
Cuts at: 513 1065 2520 513
Size: 552 1455 5149
Fragments arranged by size:
5149 1455 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6417
Fragments arranged by size:
6417 683 56
BtsI GCAGTG_nn'
Cuts at: 2020 2377 4156 4672 5466 5486 2020
Size: 357 1779 516 794 20 3690
Fragments arranged by size:
3690 1779 794 516 357 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7121
DraI TTT'AAA
Cuts at: 4460 4460
Size: 7156
DraIII CAC_nnn'GTG
Cuts at: 4030 4030
Size: 7156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2105 6163 809
Size: 507 789 4058 1802
Fragments arranged by size:
4058 1802 789 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2239 2321
Size: 54 1425 50 275 18 231 178 82
Cuts at: 2321 2931 3370 4603 5492 7121 8
Size: 610 439 1233 889 1629 43
Fragments arranged by size:
1629 1425 1233 889 610 439 275 231
178 82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2239 4603 1537
Size: 275 427 2364 4090
Fragments arranged by size:
4090 2364 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3692 4371 5086 1353
Size: 2339 679 715 3423
Fragments arranged by size:
3423 2339 715 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5743 6117 6263 387
Size: 1064 138 4154 374 146 1280
Fragments arranged by size:
4154 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3474 3722 3474
Size: 248 6908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 2633 2918 3426 4343 5219 5816
Size: 393 784 217 285 508 917 876 597
Cuts at: 5816 6603 6871 1239
Size: 787 268 1524
Fragments arranged by size:
1524 917 876 787 784 597 508 393
285 268 217
EcoO109I rG'GnC_Cy
Cuts at: 2624 2699 3486 3510 3525 3526 4435 2624
Size: 75 787 24 15 1 909 5345
Fragments arranged by size:
5345 909 787 75 24 15 1
EcoRI G'AATT_C
Cuts at: 4465 4843 4465
Size: 378 6778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3529 4226 3529
Size: 697 6459
FseI GG_CCGG'CC
Cuts at: 3372 3372
Size: 7156
FspI TGC'GCA
Cuts at: 3284 5662 3284
Size: 2378 4778
HaeII r_GCGC'y
Cuts at: 1678 2077 2145 2612 6303 1678
Size: 399 68 467 3691 2531
Fragments arranged by size:
3691 2531 467 399 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3282 3314 3923 3955 4400
Size: 32 135 32 1710 32 609 32 445
Cuts at: 4400 4432 5797 5829 5871 5903 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1710 1365 609 445 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 4500 4746 669
Size: 988 281 748 1814 246 3079
Fragments arranged by size:
3079 1814 988 748 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7156
HpaI GTT'AAC
Cuts at: 4746 4746
Size: 7156
KasI G'GCGC_C
Cuts at: 2073 2141 2073
Size: 68 7088
KpnI G_GTAC'C
Cuts at: 4481 4481
Size: 7156
MfeI C'AATT_G
Cuts at: 4755 4755
Size: 7156
MluI A'CGCG_T
Cuts at: 7032 7032
Size: 7156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 6270 6454 1305
Size: 1254 3711 184 2007
Fragments arranged by size:
3711 2007 1254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2323 2933 10
Size: 54 1425 343 491 610 4233
Fragments arranged by size:
4233 1425 610 491 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 5114 5473 5632 518
Size: 648 2220 1728 359 159 2042
Fragments arranged by size:
2220 2042 1728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2307 2316 2516 3263 3353 3558 4572 5249
Size: 701 9 200 747 90 205 1014 677
Cuts at: 5249 6397 6642 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1014 747 701 677 245 205
200 90 9
NaeI GCC'GGC
Cuts at: 3370 3370
Size: 7156
NarI GG'CG_CC
Cuts at: 2074 2142 2074
Size: 68 7088
NcoI C'CATG_G
Cuts at: 513 1065 2520 513
Size: 552 1455 5149
Fragments arranged by size:
5149 1455 552
NdeI CA'TA_TG
Cuts at: 387 3850 4282 387
Size: 3463 432 3261
Fragments arranged by size:
3463 3261 432
NgoMIV G'CCGG_C
Cuts at: 3368 3368
Size: 7156
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7156
NotI GC'GGCC_GC
Cuts at: 4603 4603
Size: 7156
NspI r_CATG'y
Cuts at: 1132 1229 2383 2965 3127 3608 4514 6059 1132
Size: 97 1154 582 162 481 906 1545 2229
Fragments arranged by size:
2229 1545 1154 906 582 481 162 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7156
PciI A'CATG_T
Cuts at: 2961 6055 2961
Size: 3094 4062
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2319 4030 1108
Size: 1211 1711 4234
Fragments arranged by size:
4234 1711 1211
PmeI GTTT'AAAC
Cuts at: 4460 4460
Size: 7156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5225 5257 6738 6770 5225
Size: 32 1481 32 5611
Fragments arranged by size:
5611 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3486 3510 4435 3486
Size: 24 925 6207
Fragments arranged by size:
6207 925 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7156
PsiI TTA'TAA
Cuts at: 4386 4726 4386
Size: 340 6816
PspOMI G'GGCC_C
Cuts at: 2699 3525 3904 2699
Size: 826 379 5951
Fragments arranged by size:
5951 826 379
PspXI vC'TCGA_Gb
Cuts at: 2078 2078
Size: 7156
PstI C_TGCA'G
Cuts at: 830 2865 3352 4200 4508 830
Size: 2035 487 848 308 3478
Fragments arranged by size:
3478 2035 848 487 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7156
PvuII CAG'CTG
Cuts at: 2316 2516 3263 3353 3558 2316
Size: 200 747 90 205 5914
Fragments arranged by size:
5914 747 205 200 90
SacI G_AGCT'C
Cuts at: 3476 3724 3476
Size: 248 6908
SalI G'TCGA_C
Cuts at: 1936 4498 1936
Size: 2562 4594
SanDI GG'GwC_CC
Cuts at: 3486 4435 3486
Size: 949 6207
SbfI CC_TGCA'GG
Cuts at: 4508 4508
Size: 7156
ScaI AGT'ACT
Cuts at: 4343 5404 7116 4343
Size: 1061 1712 4383
Fragments arranged by size:
4383 1712 1061
SfcI C'TryA_G
Cuts at: 826 1045 2164 2819 2861 3348 4196 4504 5639
Size: 219 1119 655 42 487 848 308 1135
Cuts at: 5639 6320 6511 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1119 848 681 655 487 308
219 191 42
SfoI GGC'GCC
Cuts at: 2075 2143 2075
Size: 68 7088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7156
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7156
SmaI CCC'GGG
Cuts at: 1872 2277 3786 4483 1872
Size: 405 1509 697 4545
Fragments arranged by size:
4545 1509 697 405
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2405 5260 6161 6423 820
Size: 197 926 135 327 2855 901 262 1553
Fragments arranged by size:
2855 1553 926 901 327 262 197 135
SnaBI TAC'GTA
Cuts at: 493 4947 493
Size: 4454 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7156
SphI G_CATG'C
Cuts at: 1132 4514 1132
Size: 3382 3774
SspI AAT'ATT
Cuts at: 5 52 5080 5
Size: 47 5028 2081
Fragments arranged by size:
5028 2081 47
StuI AGG'CCT
Cuts at: 1517 2709 3189 3889 1517
Size: 1192 480 700 4784
Fragments arranged by size:
4784 1192 700 480
StyI C'CwwG_G
Cuts at: 513 1065 2217 2520 3000 4595 513
Size: 552 1152 303 480 1595 3074
Fragments arranged by size:
3074 1595 1152 552 480 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3933 5183 5200 5353 5538 5983 325
Size: 1255 2353 1250 17 153 185 445 1498
Fragments arranged by size:
2353 1498 1255 1250 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3396 4341 4357
Size: 274 80 33 51 1443 1419 945 16
Cuts at: 4357 5402 7114 96
Size: 1045 1712 138
Fragments arranged by size:
1712 1443 1419 1045 945 274 138 80
51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3954 3986 7116 7148 3164
Size: 32 758 32 3130 32 3172
Fragments arranged by size:
3172 3130 758 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3920 1316
Size: 2604 4552
XbaI T'CTAG_A
Cuts at: 4492 4492
Size: 7156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3124 3124
Size: 7156
XhoI C'TCGA_G
Cuts at: 1943 2078 1943
Size: 135 7021
XmaI C'CCGG_G
Cuts at: 1870 2275 3784 4481 1870
Size: 405 1509 697 4545
Fragments arranged by size:
4545 1509 697 405
XmnI GAAnn'nnTTC
Cuts at: 5285 5285
Size: 7156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4964 276
Size: 53 83 186 936 3430 2468
Fragments arranged by size:
3430 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FspAI NruI NsiI PacI PfoI
PmlI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI