Restriction map for
pFN21AB4220
(Circular) (Six-base) MAPSORT of: pFN21AB4220.seq Check: 2471 from: 1 to: 5746
pFN21AB4220 5746 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2623 3089 1937
Size: 686 466 4594
Fragments arranged by size:
4594 686 466
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2250 2357 3809 5193 5461 1632
Size: 608 10 107 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 608 268 107 10
AflII C'TTAA_G
Cuts at: 820 1017 2114 820
Size: 197 1097 4452
Fragments arranged by size:
4452 1097 197
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2940 1184
Size: 1756 3990
AlwNI CAG_nnn'CTG
Cuts at: 2578 3489 5061 2578
Size: 911 1572 3263
Fragments arranged by size:
3263 1572 911
ApaI G_GGCC'C
Cuts at: 2908 2908
Size: 5746
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2862 3071 1870
Size: 73 919 209 4545
Fragments arranged by size:
4545 919 209 73
BamHI G'GATC_C
Cuts at: 2897 3076 3443 2897
Size: 179 367 5200
Fragments arranged by size:
5200 367 179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2670 3067 4522 618
Size: 325 217 627 883 397 1455 1842
Fragments arranged by size:
1842 1455 883 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 2908 3065 1391
Size: 1452 65 157 4072
Fragments arranged by size:
4072 1452 157 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2839 928
Size: 971 940 3835
Fragments arranged by size:
3835 971 940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2908 3807 4963 1790
Size: 1118 899 1156 2573
Fragments arranged by size:
2573 1156 1118 899
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2641 2743 3477
Size: 416 53 83 399 24 1224 102 734
Cuts at: 3477 4435 442
Size: 958 1753
Fragments arranged by size:
1753 1224 958 734 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2933 4406 1239
Size: 1694 1473 2579
Fragments arranged by size:
2579 1694 1473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 4409 882
Size: 617 792 2118 2219
Fragments arranged by size:
2219 2118 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 2997 5740 2997
Size: 2743 3003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2433 4179 4577 4851 4998 5409 2433
Size: 1746 398 274 147 411 2770
Fragments arranged by size:
2770 1746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2548 2578 1178
Size: 30 165 30 1145 30 4346
Fragments arranged by size:
4346 1145 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3029 3032 3041 1186
Size: 1843 3 9 3891
Fragments arranged by size:
3891 1843 9 3
BseYI C'CCAG_C
Cuts at: 2647 2907 4949 5445 2647
Size: 260 2042 496 2948
Fragments arranged by size:
2948 2042 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 1163
Size: 573 896 4277
Fragments arranged by size:
4277 896 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2527 2843 2919 3807 3892 4963 2527
Size: 316 76 888 85 1071 3310
Fragments arranged by size:
3310 1071 888 316 85 76
BsmI GAATG_Cn'
Cuts at: 2591 3257 3350 2591
Size: 666 93 4987
Fragments arranged by size:
4987 666 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2527 2843 2908 2919 3065 3807 3892
Size: 399 737 316 65 11 146 742 85
Cuts at: 3892 4963 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 737 399 316 146 85
65 11
BspEI T'CCGG_A
Cuts at: 2433 5409 2433
Size: 2976 2770
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3042 4241 4415 65
Size: 1183 1794 1199 174 1396
Fragments arranged by size:
1794 1396 1199 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2783 1184
Size: 1599 4147
BstXI CCAn_nnnn'nTGG
Cuts at: 2087 2408 2901 2087
Size: 321 493 4932
Fragments arranged by size:
4932 493 321
BstYI r'GATC_y
Cuts at: 1972 2897 3076 3443 3828 3845 5297 5383 5395
Size: 925 179 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 925 385 367 346 179 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2214 2214
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2014 2049 529
Size: 35 1450 35 4226
Fragments arranged by size:
4226 1450 35 35
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 2198 2198
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2424 4753 809
Size: 507 1108 2329 1802
Fragments arranged by size:
2329 1802 1108 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2707 3193 4082
Size: 54 1425 50 275 18 877 486 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 877 486 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3018 3676 1353
Size: 1665 658 3423
Fragments arranged by size:
3423 1665 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 2841
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2250 2357 2933 3809 4406 5193
Size: 393 608 10 107 576 876 597 787
Cuts at: 5193 5461 1239
Size: 268 1524
Fragments arranged by size:
1524 876 787 608 597 576 393 268
107 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2412 2882 2412
Size: 470 5276
EcoO109I rG'GnC_Cy
Cuts at: 2092 2218 2234 2483 2092
Size: 126 16 249 5355
Fragments arranged by size:
5355 249 126 16
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2845 2877 2882 2914 2963
Size: 32 135 32 1273 32 5 32 49
Cuts at: 2963 2995 4387 4419 4461 4493 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1273 135 49 42 32 32
32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2207 748
Size: 1459 4287
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2709 10
Size: 54 1425 343 877 3047
Fragments arranged by size:
3047 1425 877 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 2922 3704 4063 4222 518
Size: 648 1662 94 782 359 159 2042
Fragments arranged by size:
2042 1662 782 648 359 159 94
MspA1I CmG'CkG
Cuts at: 1606 2448 2911 3162 3839 4987 5232 1606
Size: 842 463 251 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 463 251 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2087 1348
Size: 739 5007
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 2578 1108
Size: 1260 210 4276
Fragments arranged by size:
4276 1260 210
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2092
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2904 2904
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2448 2911 2448
Size: 463 5283
SacI G_AGCT'C
Cuts at: 2843 2843
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SanDI GG'GwC_CC
Cuts at: 2092 2092
Size: 5746
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2145 3094 4229 4910 5101 826
Size: 219 1100 949 1135 681 191 1471
Fragments arranged by size:
1471 1135 1100 949 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 3850 4751 5013 820
Size: 197 926 171 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2479 3185 513
Size: 552 1414 706 3074
Fragments arranged by size:
3074 1414 706 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2532 2725 3773 3790 3943 4128 4573
Size: 1255 952 193 1048 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1048 952 445 193 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2180 3992 5704
Size: 274 80 33 51 1443 203 1812 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1812 1712 1443 274 203 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2517 2548 2549 2580 5706 5738 2517
Size: 31 1 31 3126 32 2525
Fragments arranged by size:
3126 2525 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI