Restriction map for
pFN21AB4216
(Circular) (Six-base) MAPSORT of: pFN21AB4216.seq Check: 437 from: 1 to: 8128
pFN21AB4216 8128 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 5283 2599
Size: 2684 5444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4640 5938 278
Size: 53 83 186 936 3104 1298 2468
Fragments arranged by size:
3104 2468 1298 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3435 5471 1937
Size: 1498 2036 4594
Fragments arranged by size:
4594 2036 1498
Acc65I G'GTAC_C
Cuts at: 2950 5449 2950
Size: 2499 5629
AclI AA'CG_TT
Cuts at: 1563 6255 6628 1563
Size: 4692 373 3063
Fragments arranged by size:
4692 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2247 2381 2531 2798 3206 3281 6191 7575
Size: 615 134 150 267 408 75 2910 1384
Cuts at: 7575 7843 1632
Size: 268 1917
Fragments arranged by size:
2910 1917 1384 615 408 268 267 150
134 75
AfeI AGC'GCT
Cuts at: 4959 4959
Size: 8128
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7931
AflIII A'CryG_T
Cuts at: 1203 5010 7027 8004 1203
Size: 3807 2017 977 1327
Fragments arranged by size:
3807 2017 1327 977
AgeI A'CCGG_T
Cuts at: 6959 6959
Size: 8128
AhdI GACnn_n'nnGTC
Cuts at: 3319 6857 3319
Size: 3538 4590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2688 2712 4439 4799 1184
Size: 1504 24 1727 360 4513
Fragments arranged by size:
4513 1727 1504 360 24
AlwNI CAG_nnn'CTG
Cuts at: 2699 3311 3554 3587 3782 3956 4240 5021 5164
Size: 612 243 33 195 174 284 781 143
Cuts at: 5164 5871 7443 2699
Size: 707 1572 3384
Fragments arranged by size:
3384 1572 781 707 612 284 243 195
174 143 33
ApaI G_GGCC'C
Cuts at: 3523 3523
Size: 8128
ApaLI G'TGCA_C
Cuts at: 6185 7341 6185
Size: 1156 6972
ApoI r'AATT_y
Cuts at: 1493 4502 5437 5663 5815 7924 1493
Size: 3009 935 226 152 2109 1697
Fragments arranged by size:
3009 2109 1697 935 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6682 160
Size: 872 5650 1606
Fragments arranged by size:
5650 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8128
AvaI C'yCGr_G
Cuts at: 1870 1943 3993 4250 5299 5453 1870
Size: 73 2050 257 1049 154 4545
Fragments arranged by size:
4545 2050 1049 257 154 73
AvrII C'CTAG_G
Cuts at: 3229 3229
Size: 8128
BamHI G'GATC_C
Cuts at: 4058 5458 5825 4058
Size: 1400 367 6361
Fragments arranged by size:
6361 1400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 3732 5449 6904 618
Size: 325 217 627 1163 782 1717 1455 1842
Fragments arranged by size:
1842 1717 1455 1163 782 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2979 3523 4176 4234 4571 4589 4748 5447
Size: 1588 544 653 58 337 18 159 699
Cuts at: 5447 1391
Size: 4072
Fragments arranged by size:
4072 1588 699 653 544 337 159 58
18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2786 3919 3978 928
Size: 971 887 1133 59 5078
Fragments arranged by size:
5078 1133 971 887 59
BbvCI CC'TCA_GC
Cuts at: 4223 4223
Size: 8128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4241 4275 6319 6353 1903
Size: 34 2304 34 2044 34 3678
Fragments arranged by size:
3678 2304 2044 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6020 7230 6020
Size: 1210 6918
BclI T'GATC_A
Cuts at: 1548 2457 1548
Size: 909 7219
BfrBI ATG'CAT
Cuts at: 4786 4786
Size: 8128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3959 4245 4988
Size: 107 122 71 631 605 2287 286 743
Cuts at: 4988 6739 136
Size: 1751 1525
Fragments arranged by size:
2287 1751 1525 743 631 605 286 122
107 71
BglII A'GATC_T
Cuts at: 3748 8123 3748
Size: 4375 3753
BlpI GC'TnA_GC
Cuts at: 5545 5545
Size: 8128
Bme1580I G_kGCm'C
Cuts at: 1790 2940 3523 3826 4369 6189 7345 1790
Size: 1150 583 303 543 1820 1156 2573
Fragments arranged by size:
2573 1820 1156 1150 583 543 303
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5859 6817 442
Size: 416 53 83 399 24 4442 958 1753
Fragments arranged by size:
4442 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 8128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 3748 4448 4492 4497 4538 4564 4652
Size: 815 1694 700 44 5 41 26 88
Cuts at: 4652 5363 6788 1239
Size: 711 1425 2579
Fragments arranged by size:
2579 1694 1425 815 711 700 88 44
41 26 5
Bpu10I CC'TnA_GC
Cuts at: 3653 4223 3653
Size: 570 7558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2538 2993 3902 4160 4580 6253 7118 7416 2538
Size: 455 909 258 420 1673 865 298 3250
Fragments arranged by size:
3250 1673 909 865 455 420 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2039 4473 4994 6791 882
Size: 617 531 9 2434 521 1797 2219
Fragments arranged by size:
2434 2219 1797 617 531 521 9
BsaAI yAC'GTr
Cuts at: 493 1204 4912 5919 493
Size: 711 3708 1007 2702
Fragments arranged by size:
3708 2702 1007 711
BsaBI GATnn'nnATC
Cuts at: 3753 8122 3753
Size: 4369 3759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3087 4637 5935 6317
Size: 53 83 186 936 1554 1550 1298 382
Cuts at: 6317 6952 275
Size: 635 1451
Fragments arranged by size:
1554 1550 1451 1298 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3735 6561 6959 7233 7380 7791 3735
Size: 2826 398 274 147 411 4072
Fragments arranged by size:
4072 2826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3695 3725 5348 5378 1178
Size: 30 165 30 2292 30 1623 30 3928
Fragments arranged by size:
3928 2292 1623 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3025 3402 3703 3780 4133 5284 1186
Size: 1839 377 301 77 353 1151 4030
Fragments arranged by size:
4030 1839 1151 377 353 301 77
BseYI C'CCAG_C
Cuts at: 2023 2059 2409 2415 2628 3304 3424 4198 4378
Size: 36 350 6 213 676 120 774 180
Cuts at: 4378 5083 5398 7331 7827 2023
Size: 705 315 1933 496 2324
Fragments arranged by size:
2324 1933 774 705 676 496 350 315
213 180 120 36 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2633 2708 2732 4007 4016 4134 1163
Size: 573 897 75 24 1275 9 118 5157
Fragments arranged by size:
5157 1275 897 573 118 75 24 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5359 5578 6339 7367 1540
Size: 275 186 3358 219 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 219 186
BsiHKAI G_wGCw'C
Cuts at: 4139 4257 4516 4589 4596 5201 6189 6274 7345
Size: 118 259 73 7 605 988 85 1071
Cuts at: 7345 4139
Size: 4922
Fragments arranged by size:
4922 1071 988 605 259 118 85 73
7
BsmI GAATG_Cn'
Cuts at: 2527 5639 5732 2527
Size: 3112 93 4923
Fragments arranged by size:
4923 3112 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4668 4668
Size: 8128
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2940 2979 3523 3826 4139 4176 4234
Size: 399 1150 39 544 303 313 37 58
Cuts at: 4234 4257 4369 4516 4571 4589 4596 4748 5201
Size: 23 112 147 55 18 7 152 453
Cuts at: 5201 5447 6189 6274 7345 1391
Size: 246 742 85 1071 2174
Fragments arranged by size:
2174 1150 1071 742 544 453 399 313
303 246 152 147 112 85 58 55
39 37 23 18 7
BspEI T'CCGG_A
Cuts at: 7791 7791
Size: 8128
BspHI T'CATG_A
Cuts at: 6017 7747 6017
Size: 1730 6398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 2707 2938 3139 3809 4387 4431 4944
Size: 1755 108 231 201 670 578 44 513
Cuts at: 4944 5283 5483 844
Size: 339 200 3489
Fragments arranged by size:
3489 1755 670 578 513 339 231 201
200 108 44
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 4321 5242 6015 1394
Size: 429 1618 880 921 773 3507
Fragments arranged by size:
3507 1618 921 880 773 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2233 4389 4834 5415 6623 6797 65
Size: 1183 985 2156 445 581 1208 174 1396
Fragments arranged by size:
2156 1396 1208 1183 985 581 445 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2590 3785 3936 4017 5358 6772 6959
Size: 110 780 1195 151 81 1341 1414 187
Cuts at: 6959 1700
Size: 2869
Fragments arranged by size:
2869 1414 1341 1195 780 187 151 110
81
BsrGI T'GTAC_A
Cuts at: 96 4075 96
Size: 3979 4149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8128
BssSI C'ACGA_G
Cuts at: 6188 7200 6188
Size: 1012 7116
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2611 3782 4030 5376 794
Size: 1817 1171 248 1346 3546
Fragments arranged by size:
3546 1817 1346 1171 248
BstBI TT'CG_AA
Cuts at: 6932 6932
Size: 8128
BstEII G'GTnAC_C
Cuts at: 1184 2593 3718 1184
Size: 1409 1125 5594
Fragments arranged by size:
5594 1409 1125
BstXI CCAn_nnnn'nTGG
Cuts at: 4516 4729 5260 4516
Size: 213 531 7384
Fragments arranged by size:
7384 531 213
BstYI r'GATC_y
Cuts at: 1972 3748 3754 4058 4125 4149 5458 5825 6210
Size: 1776 6 304 67 24 1309 367 385
Cuts at: 6210 6227 7679 7765 7777 8123 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1776 1452 1309 385 367 346 304
86 67 24 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 4458 4458
Size: 8128
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 4167 513
Size: 552 939 36 2127 4474
Fragments arranged by size:
4474 2127 939 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7389
Fragments arranged by size:
7389 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2209 2893 3515 3568 3824 5319 5409 5644
Size: 30 684 622 53 256 1495 90 235
Cuts at: 5644 6438 6458 2179
Size: 794 20 3849
Fragments arranged by size:
3849 1495 794 684 622 256 235 90
53 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2859 2894 4632 4667 4861 4896 529
Size: 35 2295 35 1738 35 194 35 3761
Fragments arranged by size:
3761 2295 1738 194 35 35 35 35
DraI TTT'AAA
Cuts at: 5432 5432
Size: 8128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7135 809
Size: 507 5819 1802
Fragments arranged by size:
5819 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2649 3556 3698
Size: 54 1425 50 275 18 819 907 142
Cuts at: 3698 4209 4653 4852 5230 5322 5575 6464 8093
Size: 511 444 199 378 92 253 889 1629
Cuts at: 8093 8
Size: 43
Fragments arranged by size:
1629 1425 907 889 819 511 444 378
275 253 199 142 92 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5575 1537
Size: 275 3763 4090
Fragments arranged by size:
4090 3763 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3624 4485 6058 1353
Size: 2271 861 1573 3423
Fragments arranged by size:
3423 2271 1573 861
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3013 4051 4591 5069 6715 7089
Size: 1064 138 1424 1038 540 478 1646 374
Cuts at: 7089 7235 387
Size: 146 1280
Fragments arranged by size:
1646 1424 1280 1064 1038 540 478 374
146 138
EcoICRI GAG'CTC
Cuts at: 4587 4587
Size: 8128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2247 2381 2531 2798 3206 3281
Size: 393 422 193 134 150 267 408 75
Cuts at: 3281 3748 4448 4492 4497 4538 4564 4652 5363
Size: 467 700 44 5 41 26 88 711
Cuts at: 5363 6191 6788 7575 7843 1239
Size: 828 597 787 268 1524
Fragments arranged by size:
1524 828 787 711 700 597 467 422
408 393 268 267 193 150 134 88
75 44 41 26 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3727 4754 5345 3727
Size: 1027 591 6510
Fragments arranged by size:
6510 1027 591
EcoO109I rG'GnC_Cy
Cuts at: 2719 3321 3519 4726 5044 2719
Size: 602 198 1207 318 5803
Fragments arranged by size:
5803 1207 602 318 198
EcoRI G'AATT_C
Cuts at: 4502 5437 5815 4502
Size: 935 378 6815
Fragments arranged by size:
6815 935 378
EcoRV GAT'ATC
Cuts at: 3029 3029
Size: 8128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3943 4219 4780 3943
Size: 276 561 7291
Fragments arranged by size:
7291 561 276
FspI TGC'GCA
Cuts at: 4395 5060 6634 4395
Size: 665 1574 5889
Fragments arranged by size:
5889 1574 665
HaeII r_GCGC'y
Cuts at: 1678 3425 3993 4961 5022 5365 7275 1678
Size: 1747 568 968 61 343 1910 2531
Fragments arranged by size:
2531 1910 1747 968 568 343 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3305 3337 3601 3633 5348
Size: 32 135 32 1733 32 264 32 1715
Cuts at: 5348 5380 6769 6801 6843 6875 1373
Size: 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1733 1715 1389 264 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5472 5718 669
Size: 988 281 3534 246 3079
Fragments arranged by size:
3534 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3209 4046 748
Size: 2461 837 4830
Fragments arranged by size:
4830 2461 837
HpaI GTT'AAC
Cuts at: 5718 5718
Size: 8128
KpnI G_GTAC'C
Cuts at: 2954 5453 2954
Size: 2499 5629
MfeI C'AATT_G
Cuts at: 5727 5727
Size: 8128
MluI A'CGCG_T
Cuts at: 5010 8004 5010
Size: 2994 5134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2357 5062 7242 7426 1305
Size: 1052 2705 2180 184 2007
Fragments arranged by size:
2705 2180 2007 1052 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4211 4655 4854 10
Size: 54 1425 343 2379 444 199 3284
Fragments arranged by size:
3284 2379 1425 444 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6086 6445 6604 518
Size: 648 4920 359 159 2042
Fragments arranged by size:
4920 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2152 2196 2247 2437 2632 3779 4030
Size: 410 136 44 51 190 195 1147 251
Cuts at: 4030 4343 4382 4662 4685 4921 5161 5308 5544
Size: 313 39 280 23 236 240 147 236
Cuts at: 5544 6221 7369 7614 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1147 677 410 313 280 251
245 240 236 236 195 190 147 136
51 44 39 23
NaeI GCC'GGC
Cuts at: 3787 3938 3787
Size: 151 7977
NcoI C'CATG_G
Cuts at: 513 1065 2004 2040 4167 513
Size: 552 939 36 2127 4474
Fragments arranged by size:
4474 2127 939 552 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8128
NgoMIV G'CCGG_C
Cuts at: 3785 3936 3785
Size: 151 7977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8128
NotI GC'GGCC_GC
Cuts at: 5575 5575
Size: 8128
NsiI A_TGCA'T
Cuts at: 4788 4788
Size: 8128
NspI r_CATG'y
Cuts at: 1132 1229 4790 5486 7031 1132
Size: 97 3561 696 1545 2229
Fragments arranged by size:
3561 2229 1545 696 97
PasI CC'CwG_GG
Cuts at: 1348 2077 2379 4297 4606 4799 1348
Size: 729 302 1918 309 193 4677
Fragments arranged by size:
4677 1918 729 309 302 193
PciI A'CATG_T
Cuts at: 7027 7027
Size: 8128
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 4297 4662 4993 5164 1108
Size: 1458 1731 365 331 171 4072
Fragments arranged by size:
4072 1731 1458 365 331 171
PfoI T'CCnGG_A
Cuts at: 3863 4580 3863
Size: 717 7411
PmeI GTTT'AAAC
Cuts at: 5432 5432
Size: 8128
PmlI CAC'GTG
Cuts at: 4912 4912
Size: 8128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6197 6229 7710 7742 6197
Size: 32 1481 32 6583
Fragments arranged by size:
6583 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2719 3321 5044 2719
Size: 602 1723 5803
Fragments arranged by size:
5803 1723 602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8128
PsiI TTA'TAA
Cuts at: 5698 5698
Size: 8128
PspOMI G'GGCC_C
Cuts at: 3519 3519
Size: 8128
PstI C_TGCA'G
Cuts at: 830 2667 3510 3857 3891 4057 4778 5480 830
Size: 1837 843 347 34 166 721 702 3478
Fragments arranged by size:
3478 1837 843 721 702 347 166 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8128
PvuII CAG'CTG
Cuts at: 2152 2437 3779 4030 4343 4382 4662 4685 4921
Size: 285 1342 251 313 39 280 23 236
Cuts at: 4921 5161 2152
Size: 240 5119
Fragments arranged by size:
5119 1342 313 285 280 251 240 236
39 23
SacI G_AGCT'C
Cuts at: 4589 4589
Size: 8128
SalI G'TCGA_C
Cuts at: 1936 5470 1936
Size: 3534 4594
SanDI GG'GwC_CC
Cuts at: 3321 3321
Size: 8128
SbfI CC_TGCA'GG
Cuts at: 4057 5480 4057
Size: 1423 6705
ScaI AGT'ACT
Cuts at: 4523 6376 8088 4523
Size: 1853 1712 4563
Fragments arranged by size:
4563 1853 1712
SexAI A'CCwGG_T
Cuts at: 3069 3069
Size: 8128
SfcI C'TryA_G
Cuts at: 826 1045 2663 3506 3853 3880 3887 4053 4437
Size: 219 1618 843 347 27 7 166 384
Cuts at: 4437 4774 5476 6611 7292 7483 826
Size: 337 702 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 843 702 681 384 347
337 219 191 166 27 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8128
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8128
SmaI CCC'GGG
Cuts at: 1872 3995 5301 5455 1872
Size: 2123 1306 154 4545
Fragments arranged by size:
4545 2123 1306 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2553 2972 3917 4175 4595 6232
Size: 197 926 610 419 945 258 420 1637
Cuts at: 6232 7133 7395 820
Size: 901 262 1553
Fragments arranged by size:
1637 1553 945 926 901 610 420 419
262 258 197
SnaBI TAC'GTA
Cuts at: 493 5919 493
Size: 5426 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8128
SphI G_CATG'C
Cuts at: 1132 4790 5486 1132
Size: 3658 696 3774
Fragments arranged by size:
3774 3658 696
SspI AAT'ATT
Cuts at: 5 52 6052 5
Size: 47 6000 2081
Fragments arranged by size:
6000 2081 47
StuI AGG'CCT
Cuts at: 1517 4409 1517
Size: 2892 5236
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 3229 4167 4347 4446 4722
Size: 552 939 36 1189 938 180 99 276
Cuts at: 4722 5567 513
Size: 845 3074
Fragments arranged by size:
3074 1189 939 938 845 552 276 180
99 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 2297 2392 3555 3706 5168 5390
Size: 1255 435 282 95 1163 151 1462 222
Cuts at: 5390 6155 6172 6325 6510 6955 325
Size: 765 17 153 185 445 1498
Fragments arranged by size:
1498 1462 1255 1163 765 445 435 282
222 185 153 151 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 4075 4521
Size: 274 80 33 51 1443 643 1455 446
Cuts at: 4521 6374 8086 96
Size: 1853 1712 138
Fragments arranged by size:
1853 1712 1455 1443 643 446 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2350 2382 3695 3727 5061 5093 8088 8120 2350
Size: 32 1313 32 1334 32 2995 32 2358
Fragments arranged by size:
2995 2358 1334 1313 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3071 1316
Size: 1755 6373
XbaI T'CTAG_A
Cuts at: 5464 5464
Size: 8128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3838 4241 4990 3838
Size: 403 749 6976
Fragments arranged by size:
6976 749 403
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8128
XmaI C'CCGG_G
Cuts at: 1870 3993 5299 5453 1870
Size: 2123 1306 154 4545
Fragments arranged by size:
4545 2123 1306 154
XmnI GAAnn'nnTTC
Cuts at: 3926 4310 6257 3926
Size: 384 1947 5797
Fragments arranged by size:
5797 1947 384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4638 5936 276
Size: 53 83 186 936 3104 1298 2468
Fragments arranged by size:
3104 2468 1298 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BsiWI BstZ17I DraIII FseI
FspAI KasI NarI NruI PacI PspXI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI