Restriction map for
pFN21AB4196
(Circular) (Six-base) MAPSORT of: pFN21AB4196.seq Check: 9490 from: 1 to: 7576
pFN21AB4196 7576 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2114 2114
Size: 7576
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5386 278
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4919 1937
Size: 2982 4594
Acc65I G'GTAC_C
Cuts at: 3870 4897 3870
Size: 1027 6549
AclI AA'CG_TT
Cuts at: 1563 3373 3894 5703 6076 1563
Size: 1810 521 1809 373 3063
Fragments arranged by size:
3063 1810 1809 521 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 3524 3535 3902 4442 5639 7023 7291
Size: 677 1215 11 367 540 1197 1384 268
Cuts at: 7291 1632
Size: 1917
Fragments arranged by size:
1917 1384 1215 1197 677 540 367 268
11
AflII C'TTAA_G
Cuts at: 820 1017 2999 820
Size: 197 1982 5397
Fragments arranged by size:
5397 1982 197
AflIII A'CryG_T
Cuts at: 1203 6475 7452 1203
Size: 5272 977 1327
Fragments arranged by size:
5272 1327 977
AgeI A'CCGG_T
Cuts at: 6407 6407
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 4231 6305 4231
Size: 2074 5502
AleI CACnn'nnGTG
Cuts at: 1166 3524 3597 1166
Size: 2358 73 5145
Fragments arranged by size:
5145 2358 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3965 1184
Size: 2781 4795
AlwNI CAG_nnn'CTG
Cuts at: 2944 3739 4267 4612 5319 6891 2944
Size: 795 528 345 707 1572 3629
Fragments arranged by size:
3629 1572 795 707 528 345
ApaI G_GGCC'C
Cuts at: 2474 2474
Size: 7576
ApaLI G'TGCA_C
Cuts at: 2915 5633 6789 2915
Size: 2718 1156 3702
Fragments arranged by size:
3702 2718 1156
ApoI r'AATT_y
Cuts at: 1493 2846 2932 4031 4885 5111 5263 7372 1493
Size: 1353 86 1099 854 226 152 2109 1697
Fragments arranged by size:
2109 1697 1353 1099 854 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 6130 160
Size: 872 5098 1606
Fragments arranged by size:
5098 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
AvaI C'yCGr_G
Cuts at: 1870 1943 2466 4713 4901 1870
Size: 73 523 2247 188 4545
Fragments arranged by size:
4545 2247 523 188 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3527 3560 3862 3895 3527
Size: 33 302 33 7208
Fragments arranged by size:
7208 302 33 33
BamHI G'GATC_C
Cuts at: 2583 4906 5273 2583
Size: 2323 367 4886
Fragments arranged by size:
4886 2323 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 3625 3870 4416 4450
Size: 325 217 627 423 1415 245 546 34
Cuts at: 4450 4897 6352 618
Size: 447 1455 1842
Fragments arranged by size:
1842 1455 1415 627 546 447 423 325
245 217 34
BanII G_rGCy'C
Cuts at: 1391 2474 2781 3678 3762 4895 1391
Size: 1083 307 897 84 1133 4072
Fragments arranged by size:
4072 1133 1083 897 307 84
BbeI G_GCGC'C
Cuts at: 4420 4420
Size: 7576
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6605
BbvCI CC'TCA_GC
Cuts at: 2754 4065 4412 4446 2754
Size: 1311 347 34 5884
Fragments arranged by size:
5884 1311 347 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2088 2122 5767 5801 1903
Size: 34 50 34 67 34 3645 34 3678
Fragments arranged by size:
3678 3645 67 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4535 5468 6678 4535
Size: 933 1210 5433
Fragments arranged by size:
5433 1210 933
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6187 136
Size: 107 122 71 631 605 4515 1525
Fragments arranged by size:
4515 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7571 7571
Size: 7576
BlpI GC'TnA_GC
Cuts at: 2357 2385 3807 4993 2357
Size: 28 1422 1186 4940
Fragments arranged by size:
4940 1422 1186 28
Bme1580I G_kGCm'C
Cuts at: 1790 2474 2919 3630 3854 5637 6793 1790
Size: 684 445 711 224 1783 1156 2573
Fragments arranged by size:
2573 1783 1156 711 684 445 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 3727 3757
Size: 416 53 83 399 24 2255 55 30
Cuts at: 3757 4449 4824 5307 6265 442
Size: 692 375 483 958 1753
Fragments arranged by size:
2255 1753 958 692 483 416 399 375
83 55 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 7576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2714 2993 3761 3776 4118 4264 4385
Size: 1274 201 279 768 15 342 146 121
Cuts at: 4385 4571 4719 6236 1239
Size: 186 148 1517 2579
Fragments arranged by size:
2579 1517 1274 768 342 279 201 186
148 146 121 15
Bpu10I CC'TnA_GC
Cuts at: 2180 2502 2754 4065 4412 4446 2180
Size: 322 252 1311 347 34 5310
Fragments arranged by size:
5310 1311 347 322 252 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2139 5701 6566 6864 2139
Size: 3562 865 298 2851
Fragments arranged by size:
3562 2851 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6239 882
Size: 617 4740 2219
Fragments arranged by size:
4740 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3325 5367 493
Size: 711 2121 2042 2702
Fragments arranged by size:
2702 2121 2042 711
BsaBI GATnn'nnATC
Cuts at: 7570 7570
Size: 7576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2981 4118 4417 5383
Size: 53 83 186 936 1448 1137 299 966
Cuts at: 5383 5765 6400 275
Size: 382 635 1451
Fragments arranged by size:
1451 1448 1137 966 936 635 382 299
186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4392 6009 6407 6681 6828 7239 3873
Size: 519 1617 398 274 147 411 4210
Fragments arranged by size:
4210 1617 519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3038 3068 3749 3779 4142
Size: 30 165 30 1635 30 681 30 363
Cuts at: 4142 4172 1178
Size: 30 4582
Fragments arranged by size:
4582 1635 681 363 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2387 2546 2555 3046 3049 3395 3431
Size: 956 245 159 9 491 3 346 36
Cuts at: 3431 4451 4517 1186
Size: 1020 66 4245
Fragments arranged by size:
4245 1020 956 491 346 245 159 66
36 9 3
BseYI C'CCAG_C
Cuts at: 2165 2517 3141 3637 4599 6779 7275 2165
Size: 352 624 496 962 2180 496 2466
Fragments arranged by size:
2466 2180 962 624 496 496 352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2130 3935 1163
Size: 573 394 1805 4804
Fragments arranged by size:
4804 1805 573 394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5026 5787 6815 1540
Size: 275 186 3025 761 1028 2301
Fragments arranged by size:
3025 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 2919 3324 3836 5637 5722 6793 2817
Size: 102 405 512 1801 85 1071 3600
Fragments arranged by size:
3600 1801 1071 512 405 102 85
BsmI GAATG_Cn'
Cuts at: 5087 5180 5087
Size: 93 7483
BsmBI CGTCTCn'nnnn_
Cuts at: 4109 4547 4109
Size: 438 7138
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2474 2781 2817 2919 3324 3630 3678
Size: 399 684 307 36 102 405 306 48
Cuts at: 3678 3762 3836 3854 4895 5637 5722 6793 1391
Size: 84 74 18 1041 742 85 1071 2174
Fragments arranged by size:
2174 1071 1041 742 684 405 399 307
306 102 85 84 74 48 36 18
BspEI T'CCGG_A
Cuts at: 7239 7239
Size: 7576
BspHI T'CATG_A
Cuts at: 2053 3610 5465 7195 2053
Size: 1557 1855 1730 2434
Fragments arranged by size:
2434 1855 1730 1557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 3920 4275 4931 844
Size: 1270 1806 355 656 3489
Fragments arranged by size:
3489 1806 1270 656 355
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 4038 5463 1394
Size: 429 863 1352 1425 3507
Fragments arranged by size:
3507 1425 1352 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 3501 3673 3801 4504 6071 6245
Size: 1183 1479 774 172 128 703 1567 174
Cuts at: 6245 65
Size: 1396
Fragments arranged by size:
1567 1479 1396 1183 774 703 174 172
128
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 6220 6407 1700
Size: 110 2310 2100 187 2869
Fragments arranged by size:
2869 2310 2100 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7576
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7576
BssSI C'ACGA_G
Cuts at: 5636 6648 5636
Size: 1012 6564
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3307 3507 4093 794
Size: 2513 200 586 4277
Fragments arranged by size:
4277 2513 586 200
BstBI TT'CG_AA
Cuts at: 6380 6380
Size: 7576
BstEII G'GTnAC_C
Cuts at: 1184 3796 1184
Size: 2612 4964
BstXI CCAn_nnnn'nTGG
Cuts at: 3283 3283
Size: 7576
BstYI r'GATC_y
Cuts at: 1972 2583 2788 4365 4566 4906 5273 5658 5675
Size: 611 205 1577 201 340 367 385 17
Cuts at: 5675 7127 7213 7225 7571 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1577 1452 611 385 367 346 340
205 201 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2118 2118
Size: 7576
BtgI C'CryG_G
Cuts at: 513 1065 3768 513
Size: 552 2703 4321
Fragments arranged by size:
4321 2703 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6837
Fragments arranged by size:
6837 683 56
BtsI GCAGTG_nn'
Cuts at: 3216 3349 3854 4336 4660 5092 5886 5906 3216
Size: 133 505 482 324 432 794 20 4886
Fragments arranged by size:
4886 794 505 482 432 324 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7541
DraI TTT'AAA
Cuts at: 4880 4880
Size: 7576
DraIII CAC_nnn'GTG
Cuts at: 3416 3416
Size: 7576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6583 809
Size: 507 5267 1802
Fragments arranged by size:
5267 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2625 3561 4139
Size: 54 1425 50 275 18 795 936 578
Cuts at: 4139 4335 4512 5023 5912 7541 8
Size: 196 177 511 889 1629 43
Fragments arranged by size:
1629 1425 936 889 795 578 511 275
196 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5023 1537
Size: 275 3211 4090
Fragments arranged by size:
4090 3211 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2798 3554 5506 1353
Size: 987 458 756 1952 3423
Fragments arranged by size:
3423 1952 987 756 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6163 6537 6683 387
Size: 1064 138 4574 374 146 1280
Fragments arranged by size:
4574 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2513 2714 2993 3524 3535 3761
Size: 393 677 204 201 279 531 11 226
Cuts at: 3761 3776 3902 4118 4264 4385 4442 4571 4719
Size: 15 126 216 146 121 57 129 148
Cuts at: 4719 5639 6236 7023 7291 1239
Size: 920 597 787 268 1524
Fragments arranged by size:
1524 920 787 677 597 531 393 279
268 226 216 204 201 148 146 129
126 121 57 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 4068 4068
Size: 7576
EcoO109I rG'GnC_Cy
Cuts at: 2470 2471 2941 2470
Size: 1 470 7105
Fragments arranged by size:
7105 470 1
EcoRI G'AATT_C
Cuts at: 2846 2932 4885 5263 2846
Size: 86 1953 378 5159
Fragments arranged by size:
5159 1953 378 86
FspI TGC'GCA
Cuts at: 6082 6082
Size: 7576
HaeII r_GCGC'y
Cuts at: 1678 3592 4420 6723 1678
Size: 1914 828 2303 2531
Fragments arranged by size:
2531 2303 1914 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2118 2150 2363 2395 2575
Size: 32 135 32 546 32 213 32 180
Cuts at: 2575 2607 3749 3781 4142 4174 4186 4217 4218
Size: 32 1142 32 361 32 12 31 1
Cuts at: 4218 4249 4748 4780 6217 6249 6291 6323 1373
Size: 31 499 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 1142 546 499 361 213 180
135 42 32 32 32 32 32 32
32 32 32 32 31 31 12 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 3403 3751 3898 4920 5166
Size: 988 281 711 754 348 147 1022 246
Cuts at: 5166 669
Size: 3079
Fragments arranged by size:
3079 1022 988 754 711 348 281 246
147
HindIII A'AGCT_T
Cuts at: 748 2321 748
Size: 1573 6003
HpaI GTT'AAC
Cuts at: 5166 5166
Size: 7576
KasI G'GCGC_C
Cuts at: 4416 4416
Size: 7576
KpnI G_GTAC'C
Cuts at: 3874 4901 3874
Size: 1027 6549
MfeI C'AATT_G
Cuts at: 3433 5175 3433
Size: 1742 5834
MluI A'CGCG_T
Cuts at: 7452 7452
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2231 6690 6874 1305
Size: 926 4459 184 2007
Fragments arranged by size:
4459 2007 926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 3563 4141 4337 10
Size: 54 1425 343 795 936 578 196 3249
Fragments arranged by size:
3249 1425 936 795 578 343 196 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3221 3511 3524 3597 3623 4497 5534
Size: 648 2055 290 13 73 26 874 1037
Cuts at: 5534 5893 6052 518
Size: 359 159 2042
Fragments arranged by size:
2055 2042 1037 874 648 359 290 159
73 26 13
MspA1I CmG'CkG
Cuts at: 1606 2069 2389 2581 2658 3468 3637 4445 4603
Size: 463 320 192 77 810 169 808 158
Cuts at: 4603 4992 5669 6817 7062 1606
Size: 389 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 808 677 463 389 320
245 192 169 158 77
NaeI GCC'GGC
Cuts at: 4122 4122
Size: 7576
NarI GG'CG_CC
Cuts at: 4417 4417
Size: 7576
NcoI C'CATG_G
Cuts at: 513 1065 3768 513
Size: 552 2703 4321
Fragments arranged by size:
4321 2703 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7576
NgoMIV G'CCGG_C
Cuts at: 4120 4120
Size: 7576
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7576
NotI GC'GGCC_GC
Cuts at: 5023 5023
Size: 7576
NspI r_CATG'y
Cuts at: 1132 1229 2441 3141 3510 4564 4934 6479 1132
Size: 97 1212 700 369 1054 370 1545 2229
Fragments arranged by size:
2229 1545 1212 1054 700 370 369 97
PasI CC'CwG_GG
Cuts at: 1348 3075 1348
Size: 1727 5849
PciI A'CATG_T
Cuts at: 6475 6475
Size: 7576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2944 3141 3739 3769 3774 4310 1108
Size: 1836 197 598 30 5 536 4374
Fragments arranged by size:
4374 1836 598 536 197 30 5
PfoI T'CCnGG_A
Cuts at: 3009 3738 3009
Size: 729 6847
PmeI GTTT'AAAC
Cuts at: 4880 4880
Size: 7576
PmlI CAC'GTG
Cuts at: 3325 3325
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 5677 7158 7190 5645
Size: 32 1481 32 6031
Fragments arranged by size:
6031 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7576
PsiI TTA'TAA
Cuts at: 5146 5146
Size: 7576
PspOMI G'GGCC_C
Cuts at: 2470 2470
Size: 7576
PstI C_TGCA'G
Cuts at: 830 3312 4608 4632 4666 4753 4928 830
Size: 2482 1296 24 34 87 175 3478
Fragments arranged by size:
3478 2482 1296 175 87 34 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7576
PvuII CAG'CTG
Cuts at: 2069 2389 3637 4445 4603 2069
Size: 320 1248 808 158 5042
Fragments arranged by size:
5042 1248 808 320 158
SalI G'TCGA_C
Cuts at: 1936 4918 1936
Size: 2982 4594
SbfI CC_TGCA'GG
Cuts at: 4928 4928
Size: 7576
ScaI AGT'ACT
Cuts at: 5824 7536 5824
Size: 1712 5864
SexAI A'CCwGG_T
Cuts at: 3261 3261
Size: 7576
SfcI C'TryA_G
Cuts at: 826 1045 2396 2975 3308 3841 4108 4358 4604
Size: 219 1351 579 333 533 267 250 246
Cuts at: 4604 4628 4662 4749 4924 6059 6740 6931 826
Size: 24 34 87 175 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 579 533 333 267
250 246 219 191 175 87 34 24
SfoI GGC'GCC
Cuts at: 4418 4418
Size: 7576
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7576
SmaI CCC'GGG
Cuts at: 1872 4715 4903 1872
Size: 2843 188 4545
Fragments arranged by size:
4545 2843 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2154 2999 5680 6581 6843 820
Size: 197 926 211 845 2681 901 262 1553
Fragments arranged by size:
2681 1553 926 901 845 262 211 197
SnaBI TAC'GTA
Cuts at: 493 5367 493
Size: 4874 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7576
SphI G_CATG'C
Cuts at: 1132 3510 4564 4934 1132
Size: 2378 1054 370 3774
Fragments arranged by size:
3774 2378 1054 370
SspI AAT'ATT
Cuts at: 5 52 5500 5
Size: 47 5448 2081
Fragments arranged by size:
5448 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 2507 1517
Size: 783 207 6586
Fragments arranged by size:
6586 783 207
StyI C'CwwG_G
Cuts at: 513 1065 3595 3768 5015 513
Size: 552 2530 173 1247 3074
Fragments arranged by size:
3074 2530 1247 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2538 3109 3629 4468 4651 5603 5620
Size: 1255 958 571 520 839 183 952 17
Cuts at: 5620 5773 5958 6403 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 958 952 839 571 520 445
185 183 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5822 7534 96
Size: 274 80 33 51 1443 3845 1712 138
Fragments arranged by size:
3845 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 7536 7568 3038
Size: 32 4466 32 3046
Fragments arranged by size:
4466 3046 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7576
XbaI T'CTAG_A
Cuts at: 4912 4912
Size: 7576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3026 3593 3775 4864 2011
Size: 1015 567 182 1089 4723
Fragments arranged by size:
4723 1089 1015 567 182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7576
XmaI C'CCGG_G
Cuts at: 1870 4713 4901 1870
Size: 2843 188 4545
Fragments arranged by size:
4545 2843 188
XmnI GAAnn'nnTTC
Cuts at: 3537 3887 4031 5705 3537
Size: 350 144 1674 5408
Fragments arranged by size:
5408 1674 350 144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5384 276
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI