Restriction map for
pFN21AB4192
(Circular) (Six-base) MAPSORT of: pFN21AB4192.seq Check: 2845 from: 1 to: 6184
pFN21AB4192 6184 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2694 3994 278
Size: 53 83 186 936 1158 1300 2468
Fragments arranged by size:
2468 1300 1158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2394 2550 3527 1937
Size: 457 156 977 4594
Fragments arranged by size:
4594 977 457 156
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 3385 4247 5631 5899 1632
Size: 660 1093 862 1384 268 1917
Fragments arranged by size:
1917 1384 1093 862 660 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 2985 5083 6060 1203
Size: 1782 2098 977 1327
Fragments arranged by size:
2098 1782 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 2658 1166
Size: 1492 4692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 2445 3241 3927 5499 2445
Size: 796 686 1572 3130
Fragments arranged by size:
3130 1572 796 686
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2635 2767 3083 3093 3257 3428 3509
Size: 73 692 132 316 10 164 171 81
Cuts at: 3509 1870
Size: 4545
Fragments arranged by size:
4545 692 316 171 164 132 81 73
10
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 3201 3245 3505 4960
Size: 325 217 627 876 538 44 260 1455
Cuts at: 4960 618
Size: 1842
Fragments arranged by size:
1842 1455 876 627 538 325 260 217
44
BanII G_rGCy'C
Cuts at: 1391 2017 3071 3183 3413 3503 1391
Size: 626 1054 112 230 90 4072
Fragments arranged by size:
4072 1054 626 230 112 90
BbeI G_GCGC'C
Cuts at: 2667 3249 2667
Size: 582 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2851 928
Size: 971 952 4261
Fragments arranged by size:
4261 971 952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2137 3485 4076 5286 2137
Size: 1348 591 1210 3035
Fragments arranged by size:
3035 1348 1210 591
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3066 3095 3112
Size: 107 122 71 631 605 1394 29 17
Cuts at: 3112 4795 136
Size: 1683 1525
Fragments arranged by size:
1683 1525 1394 631 605 122 107 71
29 17
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 3601 3601
Size: 6184
Bme1580I G_kGCm'C
Cuts at: 1790 2754 4245 5401 1790
Size: 964 1491 1156 2573
Fragments arranged by size:
2573 1491 1156 964
BmgBI CAC'GTC
Cuts at: 1318 2415 1318
Size: 1097 5087
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3352 3915 4873
Size: 416 53 83 399 24 1935 563 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1935 1753 958 563 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3065 3397 4844 1239
Size: 1826 332 1447 2579
Fragments arranged by size:
2579 1826 1447 332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 4309 5174 5472 2147
Size: 2162 865 298 2859
Fragments arranged by size:
2859 2162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2833 4847 882
Size: 617 1334 2014 2219
Fragments arranged by size:
2219 2014 1334 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 2234 2582 6178 2234
Size: 348 3596 2240
Fragments arranged by size:
3596 2240 348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2379 2664 2691 2999
Size: 53 83 186 936 846 285 27 308
Cuts at: 2999 3119 3246 3991 4373 5008 275
Size: 120 127 745 382 635 1451
Fragments arranged by size:
1451 936 846 745 635 382 308 285
186 127 120 83 53 27
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2219 3164 1186
Size: 838 195 945 4206
Fragments arranged by size:
4206 945 838 195
BseYI C'CCAG_C
Cuts at: 2991 5387 5883 2991
Size: 2396 496 3292
Fragments arranged by size:
3292 2396 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5611
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2763 2982 3183 3305 3399 4245 4330 5401
Size: 372 219 201 122 94 846 85 1071
Cuts at: 5401 2391
Size: 3174
Fragments arranged by size:
3174 1071 846 372 219 201 122 94
85
BsmI GAATG_Cn'
Cuts at: 3364 3695 3788 3364
Size: 331 93 5760
Fragments arranged by size:
5760 331 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2391 2754 2763 2982 3071 3183
Size: 399 227 374 363 9 219 89 112
Cuts at: 3183 3305 3399 3413 3503 4245 4330 5401 1391
Size: 122 94 14 90 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 363 227 219
122 112 94 90 89 85 14 9
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 2961 4073 5803 2961
Size: 1112 1730 3342
Fragments arranged by size:
3342 1730 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2910 3539 844
Size: 2066 629 3489
Fragments arranged by size:
3489 2066 629
BsrBI CCG'CTC
Cuts at: 1394 1823 2505 2725 2911 3389 4071 1394
Size: 429 682 220 186 478 682 3507
Fragments arranged by size:
3507 682 682 478 429 220 186
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4679 4853 65
Size: 1183 1623 1808 174 1396
Fragments arranged by size:
1808 1623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2797 2807 3204 3323 4828 5015
Size: 110 311 676 10 397 119 1505 187
Cuts at: 5015 1700
Size: 2869
Fragments arranged by size:
2869 1505 676 397 311 187 119 110
10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 2315 3182 3304 4244 5256 2315
Size: 867 122 940 1012 3243
Fragments arranged by size:
3243 1012 940 867 122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2445 794
Size: 1651 4533
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 2167 2458 1184
Size: 983 291 4910
Fragments arranged by size:
4910 983 291
BstXI CCAn_nnnn'nTGG
Cuts at: 2660 2660
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179
Size: 1542 367 385 17 1452 86 12 346
Cuts at: 6179 1972
Size: 1977
Fragments arranged by size:
1977 1542 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2139 2139
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 2004 2656 3023 3170 513
Size: 552 939 652 367 147 3527
Fragments arranged by size:
3527 939 652 552 367 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 2457 3700 4494 4514 2457
Size: 1243 794 20 4127
Fragments arranged by size:
4127 1243 794 20
ClaI AT'CG_AT
Cuts at: 1554 2238 1554
Size: 684 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2938 2973 529
Size: 35 2374 35 3740
Fragments arranged by size:
3740 2374 35 35
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 2356 2427
Size: 54 1425 50 275 18 293 233 71
Cuts at: 2427 2812 3631 4520 6149 8
Size: 385 819 889 1629 43
Fragments arranged by size:
1629 1425 889 819 385 293 275 233
71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2520 2732 3404 4114 1353
Size: 749 418 212 672 710 3423
Fragments arranged by size:
3423 749 710 672 418 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 2702 4771 5145 5291 387
Size: 1064 138 1047 66 2069 374 146 1280
Fragments arranged by size:
2069 1280 1064 1047 374 146 138 66
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 3065 3385 3397 4247 4844 5631
Size: 393 660 773 320 12 850 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 850 787 773 660 597 393 320
268 12
EcoO109I rG'GnC_Cy
Cuts at: 2026 3088 3213 3432 2026
Size: 1062 125 219 4778
Fragments arranged by size:
4778 1062 219 125
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2743 2293
Size: 450 5734
FspI TGC'GCA
Cuts at: 2449 3350 4690 2449
Size: 901 1340 3943
Fragments arranged by size:
3943 1340 901
FspAI rTGC'GCAy
Cuts at: 3350 3350
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2667 3249 5331 1678
Size: 989 582 2082 2531
Fragments arranged by size:
2531 2082 989 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3320 3352 4825 4857 4899
Size: 32 135 32 1748 32 1473 32 42
Cuts at: 4899 4931 1373
Size: 32 2626
Fragments arranged by size:
2626 1748 1473 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3528 3774 669
Size: 988 281 1590 246 3079
Fragments arranged by size:
3079 1590 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KasI G'GCGC_C
Cuts at: 2663 3245 2663
Size: 582 5602
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3363 5298 5482 1305
Size: 2058 1935 184 2007
Fragments arranged by size:
2058 2007 1935 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2358 10
Size: 54 1425 343 526 3836
Fragments arranged by size:
3836 1425 526 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2658 2747 2960 4142 4501 4660 518
Size: 648 1492 89 213 1182 359 159 2042
Fragments arranged by size:
2042 1492 1182 648 359 213 159 89
MspA1I CmG'CkG
Cuts at: 1606 2995 3025 3267 3600 4277 5425 5670 1606
Size: 1389 30 242 333 677 1148 245 2120
Fragments arranged by size:
2120 1389 1148 677 333 245 242 30
NaeI GCC'GGC
Cuts at: 2123 2799 2809 2123
Size: 676 10 5498
Fragments arranged by size:
5498 676 10
NarI GG'CG_CC
Cuts at: 2664 3246 2664
Size: 582 5602
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4693
Fragments arranged by size:
4693 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NgoMIV G'CCGG_C
Cuts at: 2121 2797 2807 2121
Size: 676 10 5498
Fragments arranged by size:
5498 676 10
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2678 1348
Size: 1330 4854
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3241 1108
Size: 2133 4051
PfoI T'CCnGG_A
Cuts at: 3042 3042
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 4253 4285 5766 5798 2519
Size: 32 1702 32 1481 32 2905
Fragments arranged by size:
2905 1702 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 3213 2026
Size: 1187 4997
PshAI GACnn'nnGTC
Cuts at: 1613 2690 1613
Size: 1077 5107
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3536 830
Size: 2706 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6184
SacII CC_GC'GG
Cuts at: 3026 3026
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SapI GCTCTTCn'nnn_
Cuts at: 2732 3404 2732
Size: 672 5512
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2263 3532 4667 5348 5539 826
Size: 219 1218 1269 1135 681 191 1471
Fragments arranged by size:
1471 1269 1218 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3095 3095
Size: 6184
SfoI GGC'GCC
Cuts at: 2665 3247 2665
Size: 582 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 3323 1700
Size: 1623 4561
SmaI CCC'GGG
Cuts at: 1872 2637 3095 3511 1872
Size: 765 458 416 4545
Fragments arranged by size:
4545 765 458 416
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 4288 5189 5451 820
Size: 197 926 219 2126 901 262 1553
Fragments arranged by size:
2126 1553 926 901 262 219 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 2350 4108 5
Size: 47 2298 1758 2081
Fragments arranged by size:
2298 2081 1758 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 5104
StyI C'CwwG_G
Cuts at: 513 1065 2004 3110 3623 513
Size: 552 939 1106 513 3074
Fragments arranged by size:
3074 1106 939 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3023 3334 4211 4228 4381 4566 5011
Size: 1255 1443 311 877 17 153 185 445
Cuts at: 5011 325
Size: 1498
Fragments arranged by size:
1498 1443 1255 877 445 311 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3002 3034 6144 6176 3002
Size: 32 3110 32 3010
Fragments arranged by size:
3110 3010 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2635 3093 3509 1870
Size: 765 458 416 4545
Fragments arranged by size:
4545 765 458 416
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2692 3992 276
Size: 53 83 186 936 1158 1300 2468
Fragments arranged by size:
2468 1300 1158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SanDI SexAI SrfI SwaI XcmI