Restriction map for
pFN21AB4190
(Circular) (Six-base) MAPSORT of: pFN21AB4190.seq Check: 9476 from: 1 to: 5797
pFN21AB4190 5797 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2531 2531
Size: 5797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2744 3860 5244 5512 1632
Size: 410 702 1116 1384 268 1917
Fragments arranged by size:
1917 1384 1116 702 410 268
AfeI AGC'GCT
Cuts at: 2281 2281
Size: 5797
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 4306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 1184
Size: 1188 4609
AlwNI CAG_nnn'CTG
Cuts at: 2248 2685 2767 3030 3540 5112 2248
Size: 437 82 263 510 1572 2933
Fragments arranged by size:
2933 1572 510 437 263 82
ApaI G_GGCC'C
Cuts at: 2017 2017
Size: 5797
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2750 3122 1870
Size: 73 807 372 4545
Fragments arranged by size:
4545 807 372 73
BamHI G'GATC_C
Cuts at: 2034 3127 3494 2034
Size: 1093 367 4337
Fragments arranged by size:
4337 1093 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2579 2597 2688 3118
Size: 325 217 627 516 276 18 91 430
Cuts at: 3118 4573 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 516 430 325 276 217
91 18
BanII G_rGCy'C
Cuts at: 1391 2017 3060 3116 1391
Size: 626 1043 56 4072
Fragments arranged by size:
4072 1043 626 56
BbeI G_GCGC'C
Cuts at: 2692 2692
Size: 5797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 2452 2596 3050 928
Size: 971 466 87 144 454 3675
Fragments arranged by size:
3675 971 466 454 144 87
BbvCI CC'TCA_GC
Cuts at: 2276 2276
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2587 2621 3988 4022 1903
Size: 34 650 34 1367 34 3678
Fragments arranged by size:
3678 1367 650 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 2385 1548
Size: 837 4960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2832 4408 136
Size: 107 122 71 631 605 1160 1576 1525
Fragments arranged by size:
1576 1525 1160 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2017 2600 2797 2906 2923 3858 5014 1790
Size: 227 583 197 109 17 935 1156 2573
Fragments arranged by size:
2573 1156 935 583 227 197 109 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 3528 4486
Size: 416 53 83 399 24 741 1370 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1370 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2622 1056
Size: 1566 4231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4457 1239
Size: 3218 2579
Bpu10I CC'TnA_GC
Cuts at: 2276 2276
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2474 3922 4787 5085 2393
Size: 81 1448 865 298 3105
Fragments arranged by size:
3105 1448 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 2948 4460 882
Size: 617 644 805 1512 2219
Fragments arranged by size:
2219 1512 805 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 2851 3588 493
Size: 711 1647 737 2702
Fragments arranged by size:
2702 1647 737 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2689 3604 3986 4621
Size: 53 83 186 936 1156 915 382 635
Cuts at: 4621 275
Size: 1451
Fragments arranged by size:
1451 1156 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3049 4230 4628 4902 5049 5460 3049
Size: 1181 398 274 147 411 3386
Fragments arranged by size:
3386 1181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 4759
Fragments arranged by size:
4759 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2966 2995 3068 1186
Size: 1780 29 73 3915
Fragments arranged by size:
3915 1780 73 29
BseYI C'CCAG_C
Cuts at: 2318 2593 2781 2785 2818 2995 3030 3059 5000
Size: 275 188 4 33 177 35 29 1941
Cuts at: 5000 5496 2318
Size: 496 2619
Fragments arranged by size:
2619 1941 496 275 188 177 35 33
29 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2024 1163
Size: 573 288 4936
Fragments arranged by size:
4936 573 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 2943 3858 3943 5014 2664
Size: 279 915 85 1071 3447
Fragments arranged by size:
3447 1071 915 279 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2600 2664 2797 2906 2923 2943
Size: 399 227 583 64 133 109 17 20
Cuts at: 2943 3060 3116 3858 3943 5014 1391
Size: 117 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 399 227 133 117
109 85 64 56 20 17
BspEI T'CCGG_A
Cuts at: 3049 5460 3049
Size: 2411 3386
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2490 2531 3152 844
Size: 1352 294 41 621 3489
Fragments arranged by size:
3489 1352 621 294 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 3684 1394
Size: 429 622 1239 3507
Fragments arranged by size:
3507 1239 622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 4292 4466 65
Size: 1183 869 2175 174 1396
Fragments arranged by size:
2175 1396 1183 869 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 2152 2251 1184
Size: 968 99 4730
Fragments arranged by size:
4730 968 99
BstXI CCAn_nnnn'nTGG
Cuts at: 2167 3094 2167
Size: 927 4870
BstYI r'GATC_y
Cuts at: 1972 2034 3127 3494 3879 3896 5348 5434 5446
Size: 62 1093 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1093 385 367 346 86 62
17 12
Bsu36I CC'TnA_GG
Cuts at: 2336 2336
Size: 5797
BtgI C'CryG_G
Cuts at: 513 1065 2166 2307 3087 513
Size: 552 1101 141 780 3223
Fragments arranged by size:
3223 1101 780 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3313 4107 4127 2797
Size: 516 794 20 4467
Fragments arranged by size:
4467 794 516 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 2896 2896
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2907 2934
Size: 54 1425 50 275 18 456 621 27
Cuts at: 2934 3244 4133 5762 8
Size: 310 889 1629 43
Fragments arranged by size:
1629 1425 889 621 456 310 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3074 3727 1353
Size: 1379 342 653 3423
Fragments arranged by size:
3423 1379 653 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2998 4384 4758 4904 387
Size: 1064 138 1409 1386 374 146 1280
Fragments arranged by size:
1409 1386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2744 3860 4457 5244 5512 1239
Size: 393 410 702 1116 597 787 268 1524
Fragments arranged by size:
1524 1116 787 702 597 410 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3022 3022
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2013 2014 2058 2764 2013
Size: 1 44 706 5046
Fragments arranged by size:
5046 706 44 1
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2051 2283 2692 3087 4944 1678
Size: 373 232 409 395 1857 2531
Fragments arranged by size:
2531 1857 409 395 373 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2850 2882 4438
Size: 32 135 32 614 32 632 32 1556
Cuts at: 4438 4470 4512 4544 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1556 632 614 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 3141 3387 669
Size: 988 281 775 428 246 3079
Fragments arranged by size:
3079 988 775 428 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2688 2688
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2381 2657 2990 3092 3755 4114
Size: 648 999 216 276 333 102 663 359
Cuts at: 4114 4273 518
Size: 159 2042
Fragments arranged by size:
2042 999 663 648 359 333 276 216
159 102
MspA1I CmG'CkG
Cuts at: 1606 2785 2884 3213 3890 5038 5283 1606
Size: 1179 99 329 677 1148 245 2120
Fragments arranged by size:
2120 1179 1148 677 329 245 99
NarI GG'CG_CC
Cuts at: 2689 2689
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2166 3087 513
Size: 552 1101 921 3223
Fragments arranged by size:
3223 1101 921 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 2618 1052
Size: 1566 4231
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 4657
PfoI T'CCnGG_A
Cuts at: 2030 2030
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2013 2013
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2077 2865 3149 830
Size: 1247 788 284 3478
Fragments arranged by size:
3478 1247 788 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2785 2785
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SapI GCTCTTCn'nnn_
Cuts at: 3074 3074
Size: 5797
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2073 2861 3145 4280 4961 5152 826
Size: 219 1028 788 284 1135 681 191 1471
Fragments arranged by size:
1471 1135 1028 788 681 284 219 191
SfoI GGC'GCC
Cuts at: 2690 2690
Size: 5797
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2489 3901 4802 5064 820
Size: 197 926 465 81 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 465 262 197 81
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 2274 2915 1517
Size: 757 641 4399
Fragments arranged by size:
4399 757 641
StyI C'CwwG_G
Cuts at: 513 1065 2166 2910 3087 3236 513
Size: 552 1101 744 177 149 3074
Fragments arranged by size:
3074 1101 744 552 177 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2326
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI