Restriction map for
pFN21AB4184
(Circular) (Six-base) MAPSORT of: pFN21AB4184.seq Check: 3014 from: 1 to: 6202
pFN21AB4184 6202 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2408 2408
Size: 6202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3545 1937
Size: 1608 4594
Acc65I G'GTAC_C
Cuts at: 3523 3523
Size: 6202
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4265 5649 5917 1632
Size: 2633 1384 268 1917
Fragments arranged by size:
2633 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 5101 6078 1203
Size: 3898 977 1327
Fragments arranged by size:
3898 1327 977
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 2537 4931 2537
Size: 2394 3808
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 1184
Size: 882 5320
AlwNI CAG_nnn'CTG
Cuts at: 3945 5517 3945
Size: 1572 4630
ApaI G_GGCC'C
Cuts at: 2821 3454 3461 2821
Size: 633 7 5562
Fragments arranged by size:
5562 633 7
ApaLI G'TGCA_C
Cuts at: 4259 5415 4259
Size: 1156 5046
ApoI r'AATT_y
Cuts at: 1493 2144 3511 3737 3889 5998 1493
Size: 651 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 651 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 3527 1870
Size: 73 663 921 4545
Fragments arranged by size:
4545 921 663 73
AvrII C'CTAG_G
Cuts at: 2557 2557
Size: 6202
BamHI G'GATC_C
Cuts at: 2046 3189 3532 3899 2046
Size: 1143 343 367 4349
Fragments arranged by size:
4349 1143 367 343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 2790 3523 4978 618
Size: 325 217 627 815 188 733 1455 1842
Fragments arranged by size:
1842 1455 815 733 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2475 2498 2556 2720 2821 2871 3104 3454
Size: 1084 23 58 164 101 50 233 350
Cuts at: 3454 3461 3521 1391
Size: 7 60 4072
Fragments arranged by size:
4072 1084 350 233 164 101 60 58
50 23 7
BbeI G_GCGC'C
Cuts at: 2794 2794
Size: 6202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2401 928
Size: 971 502 4729
Fragments arranged by size:
4729 971 502
BbvCI CC'TCA_GC
Cuts at: 3155 3155
Size: 6202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4393 4427 1903
Size: 34 50 34 2372 34 3678
Fragments arranged by size:
3678 2372 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 4094 5304 2244
Size: 1850 1210 3142
Fragments arranged by size:
3142 1850 1210
BclI T'GATC_A
Cuts at: 1548 2382 1548
Size: 834 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 4813 136
Size: 107 122 71 631 605 1021 2120 1525
Fragments arranged by size:
2120 1525 1021 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3411 6197 3411
Size: 2786 3416
BlpI GC'TnA_GC
Cuts at: 2497 3619 2497
Size: 1122 5080
Bme1580I G_kGCm'C
Cuts at: 1790 2607 2788 2821 3454 3461 4263 5419 1790
Size: 817 181 33 633 7 802 1156 2573
Fragments arranged by size:
2573 1156 817 802 633 181 33 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2973 3933 4891
Size: 416 53 83 399 24 1556 960 958
Cuts at: 4891 442
Size: 1753
Fragments arranged by size:
1753 1556 960 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2196 2600 2762 2771 3497 4862 1239
Size: 957 404 162 9 726 1365 2579
Fragments arranged by size:
2579 1365 957 726 404 162 9
Bpu10I CC'TnA_GC
Cuts at: 3155 3155
Size: 6202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4327 5192 5490 4327
Size: 865 298 5039
Fragments arranged by size:
5039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3380 4865 882
Size: 617 1881 1485 2219
Fragments arranged by size:
2219 1881 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 3993 493
Size: 711 2261 528 2702
Fragments arranged by size:
2702 2261 711 528
BsaBI GATnn'nnATC
Cuts at: 6196 6196
Size: 6202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2791 4009 4391 5026
Size: 53 83 186 936 1258 1218 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1451 1258 1218 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2913 4635 5033 5307 5454 5865 2913
Size: 1722 398 274 147 411 3250
Fragments arranged by size:
3250 1722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2694 2724 2888 2918 3296
Size: 30 165 30 1291 30 164 30 378
Cuts at: 3296 3326 1178
Size: 30 4054
Fragments arranged by size:
4054 1291 378 165 164 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2493 2651 2759 2768 2771 2839 2893 3484
Size: 1307 158 108 9 3 68 54 591
Cuts at: 3484 1186
Size: 3904
Fragments arranged by size:
3904 1307 591 158 108 68 54 9
3
BseYI C'CCAG_C
Cuts at: 2287 2389 3063 5405 5901 2287
Size: 102 674 2342 496 2588
Fragments arranged by size:
2588 2342 674 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5629
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3652 4413 5441 1540
Size: 275 186 1651 761 1028 2301
Fragments arranged by size:
2301 1651 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2260 2475 2498 2556 2871 3104 4263 4348 5419
Size: 215 23 58 315 233 1159 85 1071
Cuts at: 5419 2260
Size: 3043
Fragments arranged by size:
3043 1159 1071 315 233 215 85 58
23
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 2475 2498 2556 2607 2720 2788
Size: 399 470 215 23 58 51 113 68
Cuts at: 2788 2821 2871 3104 3454 3461 3521 4263 4348
Size: 33 50 233 350 7 60 742 85
Cuts at: 4348 5419 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 350 233 215
113 85 68 60 58 51 50 33
23 7
BspEI T'CCGG_A
Cuts at: 2913 5865 2913
Size: 2952 3250
BspHI T'CATG_A
Cuts at: 4091 5821 4091
Size: 1730 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2258 2408 3068 3557 844
Size: 1414 150 660 489 3489
Fragments arranged by size:
3489 1414 660 489 150
BsrBI CCG'CTC
Cuts at: 1394 1823 4089 1394
Size: 429 2266 3507
Fragments arranged by size:
3507 2266 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 4846 5033 1700
Size: 110 262 2774 187 2869
Fragments arranged by size:
2869 2774 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 2761 1925
Size: 836 5366
BssSI C'ACGA_G
Cuts at: 2402 2843 4262 5274 2402
Size: 441 1419 1012 3330
Fragments arranged by size:
3330 1419 1012 441
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2264 3243 794
Size: 1470 979 3753
Fragments arranged by size:
3753 1470 979
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6202
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2449 2599 2287
Size: 162 150 5890
Fragments arranged by size:
5890 162 150
BstYI r'GATC_y
Cuts at: 1972 2046 2940 3189 3411 3532 3899 4284 4301
Size: 74 894 249 222 121 367 385 17
Cuts at: 4301 5753 5839 5851 6197 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 894 385 367 346 249 222
121 86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2466 2568 2822 2466
Size: 102 254 5846
Fragments arranged by size:
5846 254 102
BtgI C'CryG_G
Cuts at: 513 1065 2795 2862 3129 513
Size: 552 1730 67 267 3586
Fragments arranged by size:
3586 1730 552 267 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5463
Fragments arranged by size:
5463 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 2258 2863 3718 4512 4532 2183
Size: 75 605 855 794 20 3853
Fragments arranged by size:
3853 855 794 605 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2826 2861 529
Size: 35 2262 35 3870
Fragments arranged by size:
3870 2262 35 35
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2487 2589
Size: 54 1425 50 275 18 447 210 102
Cuts at: 2589 3126 3195 3219 3649 4538 6167 8
Size: 537 69 24 430 889 1629 43
Fragments arranged by size:
1629 1425 889 537 447 430 275 210
102 69 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3649 1537
Size: 275 1837 4090
Fragments arranged by size:
4090 1837 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4132 1353
Size: 2779 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4789 5163 5309 387
Size: 1064 138 3200 374 146 1280
Fragments arranged by size:
3200 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2473 2496 2554 2869 3102 2473
Size: 23 58 315 233 5573
Fragments arranged by size:
5573 315 233 58 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2600 2762 2771 3497 4265 4862
Size: 393 564 404 162 9 726 768 597
Cuts at: 4862 5649 5917 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 768 726 597 564 404 393
268 162 9
EcoO109I rG'GnC_Cy
Cuts at: 2270 2818 3457 3458 2270
Size: 548 639 1 5014
Fragments arranged by size:
5014 639 548 1
EcoRI G'AATT_C
Cuts at: 2144 3511 3889 2144
Size: 1367 378 4457
Fragments arranged by size:
4457 1367 378
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 2463 2615 2794 3254 5349 1678
Size: 785 152 179 460 2095 2531
Fragments arranged by size:
2531 2095 785 460 179 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 3004 3036 3296
Size: 32 135 32 951 32 449 32 260
Cuts at: 3296 3328 4843 4875 4917 4949 1373
Size: 32 1515 32 42 32 2626
Fragments arranged by size:
2626 1515 951 449 260 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3546 3792 669
Size: 988 281 1554 54 246 3079
Fragments arranged by size:
3079 1554 988 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KasI G'GCGC_C
Cuts at: 2790 2790
Size: 6202
KpnI G_GTAC'C
Cuts at: 3527 3527
Size: 6202
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2521 5316 5500 1305
Size: 1216 2795 184 2007
Fragments arranged by size:
2795 2007 1216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 2489 2591 3128 3197
Size: 54 1425 343 447 210 102 537 69
Cuts at: 3197 3221 10
Size: 24 2991
Fragments arranged by size:
2991 1425 537 447 343 210 102 69
54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 4160 4519 4678 518
Size: 648 1158 1836 359 159 2042
Fragments arranged by size:
2042 1836 1158 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2595 3618 4295 5443 5688 1606
Size: 989 1023 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 989 677 245
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 6202
NarI GG'CG_CC
Cuts at: 2791 2791
Size: 6202
NcoI C'CATG_G
Cuts at: 513 1065 2795 2862 3129 513
Size: 552 1730 67 267 3586
Fragments arranged by size:
3586 1730 552 267 67
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 3560 5105 1132
Size: 97 2331 1545 2229
Fragments arranged by size:
2331 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2239 3454 1348
Size: 891 1215 4096
Fragments arranged by size:
4096 1215 891
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6202
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PmlI CAC'GTG
Cuts at: 3465 3465
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 2270
Size: 6202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6202
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PspOMI G'GGCC_C
Cuts at: 2817 3450 3457 2817
Size: 633 7 5562
Fragments arranged by size:
5562 633 7
PstI C_TGCA'G
Cuts at: 830 3099 3554 830
Size: 2269 455 3478
Fragments arranged by size:
3478 2269 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6202
PvuII CAG'CTG
Cuts at: 2595 2595
Size: 6202
SacI G_AGCT'C
Cuts at: 2475 2498 2556 2871 3104 2475
Size: 23 58 315 233 5573
Fragments arranged by size:
5573 315 233 58 23
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 4450 6162 4450
Size: 1712 4490
SfcI C'TryA_G
Cuts at: 826 1045 3095 3550 4685 5366 5557 826
Size: 219 2050 455 1135 681 191 1471
Fragments arranged by size:
2050 1471 1135 681 455 219 191
SfoI GGC'GCC
Cuts at: 2792 2792
Size: 6202
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 2608 3529 1872
Size: 736 921 4545
Fragments arranged by size:
4545 921 736
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4306 5207 5469 820
Size: 197 926 2363 901 262 1553
Fragments arranged by size:
2363 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 2247 2573 2687 3226 3308 1517
Size: 730 326 114 539 82 4411
Fragments arranged by size:
4411 730 539 326 114 82
StyI C'CwwG_G
Cuts at: 513 1065 2274 2557 2795 2862 3129 3337 3417
Size: 552 1209 283 238 67 267 208 80
Cuts at: 3417 3641 513
Size: 224 3074
Fragments arranged by size:
3074 1209 552 283 267 238 224 208
80 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4229 4246 4399 4584 5029 325
Size: 1255 2649 17 153 185 445 1498
Fragments arranged by size:
2649 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4448 6160 96
Size: 274 80 33 51 1443 2471 1712 138
Fragments arranged by size:
2471 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6162 6194 6162
Size: 32 6170
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6202
XbaI T'CTAG_A
Cuts at: 2027 3538 2027
Size: 1511 4691
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 2606 3527 1870
Size: 736 921 4545
Fragments arranged by size:
4545 921 736
XmnI GAAnn'nnTTC
Cuts at: 4331 4331
Size: 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI