Restriction map for
pFN21AB4182
(Circular) (Six-base) MAPSORT of: pFN21AB4182.seq Check: 9342 from: 1 to: 5773
pFN21AB4182 5773 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2667 3583 278
Size: 53 83 186 936 1131 916 2468
Fragments arranged by size:
2468 1131 936 916 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 3836 5220 5488 1632
Size: 992 1212 1384 268 1917
Fragments arranged by size:
1917 1384 1212 992 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 2191 4502 2191
Size: 2311 3462
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaI G_GGCC'C
Cuts at: 2649 2998 2649
Size: 349 5424
ApaLI G'TGCA_C
Cuts at: 2039 3830 4986 2039
Size: 1791 1156 2826
Fragments arranged by size:
2826 1791 1156
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2699 2997 3098 1870
Size: 73 115 641 298 101 4545
Fragments arranged by size:
4545 641 298 115 101 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2735 2744 3094 4549
Size: 325 217 627 609 339 9 350 1455
Cuts at: 4549 618
Size: 1842
Fragments arranged by size:
1842 1455 627 609 350 339 325 217
9
BanII G_rGCy'C
Cuts at: 1391 2184 2318 2649 2998 3092 1391
Size: 793 134 331 349 94 4072
Fragments arranged by size:
4072 793 349 331 134 94
BbeI G_GCGC'C
Cuts at: 2739 2739
Size: 5773
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BbvCI CC'TCA_GC
Cuts at: 2669 2669
Size: 5773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3964 3998 1903
Size: 34 50 34 1943 34 3678
Fragments arranged by size:
3678 1943 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 2082 1548
Size: 534 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2777 4384 136
Size: 107 122 71 631 605 1105 1607 1525
Fragments arranged by size:
1607 1525 1105 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2569 5768 2569
Size: 3199 2574
BlpI GC'TnA_GC
Cuts at: 2887 3190 2887
Size: 303 5470
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2399 2480 2496 2586 2649 2998 3834
Size: 253 356 81 16 90 63 349 836
Cuts at: 3834 4990 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 836 356 349 253 90 81
63 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2867 2920 2978 4433 1239
Size: 1628 53 58 1455 2579
Fragments arranged by size:
2579 1628 1455 58 53
Bpu10I CC'TnA_GC
Cuts at: 2669 2669
Size: 5773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2995 3898 4763 5061 2000
Size: 995 903 865 298 2712
Fragments arranged by size:
2712 995 903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2595 3564 493
Size: 711 1391 969 2702
Fragments arranged by size:
2702 1391 969 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2664 2736 3580
Size: 53 83 186 936 606 525 72 844
Cuts at: 3580 3962 4597 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 844 635 606 525 382 186
83 72 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 2743 2773 2810
Size: 30 165 30 817 30 493 30 37
Cuts at: 2810 2840 2912 2942 1178
Size: 30 72 30 4009
Fragments arranged by size:
4009 817 493 165 72 37 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2498 2528 2702 2717 2975 3062 3065
Size: 1297 15 30 174 15 258 87 3
Cuts at: 3065 3068 1186
Size: 3 3891
Fragments arranged by size:
3891 1297 258 174 87 30 15 15
3 3
BseYI C'CCAG_C
Cuts at: 2095 2311 2364 2656 2876 4976 5472 2095
Size: 216 53 292 220 2100 496 2396
Fragments arranged by size:
2396 2100 496 292 220 216 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2926 1163
Size: 573 1190 4010
Fragments arranged by size:
4010 1190 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 3223 3984 5012 1540
Size: 275 186 75 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 2043 3834 3919 4990 2043
Size: 1791 85 1071 2826
Fragments arranged by size:
2826 1791 1071 85
BsiWI C'GTAC_G
Cuts at: 2595 2766 2595
Size: 171 5602
BsmI GAATG_Cn'
Cuts at: 2353 3284 3377 2353
Size: 931 93 4749
Fragments arranged by size:
4749 931 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2130 2130
Size: 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2184 2318 2399 2480 2496 2586
Size: 399 253 141 134 81 81 16 90
Cuts at: 2586 2649 2998 3092 3834 3919 4990 1391
Size: 63 349 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 253 141 134
94 90 85 81 81 63 16
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3128 844
Size: 2284 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2063 2293 3660 1394
Size: 429 240 230 1367 3507
Fragments arranged by size:
3507 1367 429 240 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 2278 2717 1184
Size: 1094 439 4240
Fragments arranged by size:
4240 1094 439
BstYI r'GATC_y
Cuts at: 1972 2560 2569 3103 3470 3855 3872 5324 5410
Size: 588 9 534 367 385 17 1452 86
Cuts at: 5410 5422 5768 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 588 534 385 367 346 86
17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2947 2947
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2385 2511 2722 2772 513
Size: 552 1320 126 211 50 3514
Fragments arranged by size:
3514 1320 552 211 126 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2478 2742 487
Size: 683 56 1252 264 3518
Fragments arranged by size:
3518 1252 683 264 56
BtsI GCAGTG_nn'
Cuts at: 2496 3289 4083 4103 2496
Size: 793 794 20 4166
Fragments arranged by size:
4166 794 793 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2125 3220
Size: 54 1425 50 275 18 243 52 1095
Cuts at: 3220 4109 5738 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1095 889 275 243 54 52
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2073 3220 1537
Size: 275 261 1147 4090
Fragments arranged by size:
4090 1147 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 2218 3703 1353
Size: 865 1485 3423
Fragments arranged by size:
3423 1485 865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 4360 4734 4880 387
Size: 1064 138 507 2264 374 146 1280
Fragments arranged by size:
2264 1280 1064 507 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 2867 2920 2978 3836 4433 5220
Size: 393 992 243 53 58 858 597 787
Cuts at: 5220 5488 1239
Size: 268 1524
Fragments arranged by size:
1524 992 858 787 597 393 268 243
58 53
EcoO109I rG'GnC_Cy
Cuts at: 2645 2646 2695 2994 2645
Size: 1 49 299 5424
Fragments arranged by size:
5424 299 49 1
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2053 2091 2739 4920 1678
Size: 375 38 648 2181 2531
Fragments arranged by size:
2531 2181 648 375 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2810 2842 2912
Size: 32 135 32 605 32 601 32 70
Cuts at: 2912 2944 4414 4446 4488 4520 1373
Size: 32 1470 32 42 32 2626
Fragments arranged by size:
2626 1470 605 601 135 70 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KasI G'GCGC_C
Cuts at: 2735 2735
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 4887 5071 1305
Size: 1175 2407 184 2007
Fragments arranged by size:
2407 2007 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2727 3731 4090 4249 518
Size: 648 1561 1004 359 159 2042
Fragments arranged by size:
2042 1561 1004 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2387 2513 2741 2870 2940 3189 3866
Size: 489 292 126 228 129 70 249 677
Cuts at: 3866 5014 5259 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 292 249 245 228
129 126 70
NarI GG'CG_CC
Cuts at: 2736 2736
Size: 5773
NcoI C'CATG_G
Cuts at: 513 1065 2722 513
Size: 552 1657 3564
Fragments arranged by size:
3564 1657 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2311 2612 3131 4676 1132
Size: 97 1082 301 519 1545 2229
Fragments arranged by size:
2229 1545 1082 519 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 1108
Size: 1737 4036
PfoI T'CCnGG_A
Cuts at: 2243 2955 2243
Size: 712 5061
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2645 2994 2645
Size: 349 5424
PstI C_TGCA'G
Cuts at: 830 3125 830
Size: 2295 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2940 2940
Size: 5773
SacII CC_GC'GG
Cuts at: 2388 2514 2388
Size: 126 5647
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SanDI GG'GwC_CC
Cuts at: 2695 2695
Size: 5773
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 3121 4256 4937 5128 826
Size: 219 2076 1135 681 191 1471
Fragments arranged by size:
2076 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2737 2737
Size: 5773
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2999 3100 1872
Size: 1127 101 4545
Fragments arranged by size:
4545 1127 101
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3010 3877 4778 5040 820
Size: 197 926 72 995 867 901 262 1553
Fragments arranged by size:
1553 995 926 901 867 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 2612 3131 1132
Size: 1480 519 3774
Fragments arranged by size:
3774 1480 519
SrfI GCCC'GGGC
Cuts at: 2999 2999
Size: 5773
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 2552 1517
Size: 1035 4738
StyI C'CwwG_G
Cuts at: 513 1065 2196 2586 2722 3212 513
Size: 552 1131 390 136 490 3074
Fragments arranged by size:
3074 1131 552 490 390 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 2768 3800 3817 3970 4155 4600
Size: 1255 1068 120 1032 17 153 185 445
Cuts at: 4600 325
Size: 1498
Fragments arranged by size:
1498 1255 1068 1032 445 185 153 120
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4019 5731 96
Size: 274 80 33 51 1443 2042 1712 138
Fragments arranged by size:
2042 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2743 2775 2843 2875 5733 5765 2743
Size: 32 68 32 2858 32 2751
Fragments arranged by size:
2858 2751 68 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 2997 3098 1870
Size: 1127 101 4545
Fragments arranged by size:
4545 1127 101
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2665 3581 276
Size: 53 83 186 936 1131 916 2468
Fragments arranged by size:
2468 1131 936 916 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SapI SexAI SfiI SwaI XcmI