Restriction map for
pFN21AB4181
(Circular) (Six-base) MAPSORT of: pFN21AB4181.seq Check: 6280 from: 1 to: 7411
pFN21AB4181 7411 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3387 5221 278
Size: 53 83 186 936 1851 1834 2468
Fragments arranged by size:
2468 1851 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3387 3546 4754 1937
Size: 1450 159 1208 4594
Fragments arranged by size:
4594 1450 1208 159
Acc65I G'GTAC_C
Cuts at: 4732 4732
Size: 7411
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 2742 3581 5474 6858 7126 1632
Size: 903 207 839 1893 1384 268 1917
Fragments arranged by size:
1917 1893 1384 903 839 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 4395 4587 6310 7287 1203
Size: 3192 192 1723 977 1327
Fragments arranged by size:
3192 1723 1327 977 192
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 6140 6140
Size: 7411
AleI CACnn'nnGTG
Cuts at: 1166 3785 3962 4382 1166
Size: 2619 177 420 4195
Fragments arranged by size:
4195 2619 420 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7411
AlwNI CAG_nnn'CTG
Cuts at: 2032 2728 3890 4574 5154 6726 2032
Size: 696 1162 684 580 1572 2717
Fragments arranged by size:
2717 1572 1162 696 684 580
ApaLI G'TGCA_C
Cuts at: 5468 6624 5468
Size: 1156 6255
ApoI r'AATT_y
Cuts at: 1493 4496 4720 4946 5098 7207 1493
Size: 3003 224 226 152 2109 1697
Fragments arranged by size:
3003 2109 1697 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 5965 160
Size: 872 4933 1606
Fragments arranged by size:
4933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 4479 4736 1870
Size: 73 828 1708 257 4545
Fragments arranged by size:
4545 1708 828 257 73
AvrII C'CTAG_G
Cuts at: 2336 2336
Size: 7411
BamHI G'GATC_C
Cuts at: 2673 3003 4741 5108 2673
Size: 330 1738 367 4976
Fragments arranged by size:
4976 1738 367 330
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2340 2692 2767 3308 3539
Size: 325 217 627 553 352 75 541 231
Cuts at: 3539 4732 6187 618
Size: 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 553 541 352 325
231 217 75
BanII G_rGCy'C
Cuts at: 1391 2244 2803 3632 4730 1391
Size: 853 559 829 1098 4072
Fragments arranged by size:
4072 1098 853 829 559
BbeI G_GCGC'C
Cuts at: 3312 3312
Size: 7411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3036 3569 3758 4395 4510 928
Size: 971 1137 533 189 637 115 3829
Fragments arranged by size:
3829 1137 971 637 533 189 115
BbvCI CC'TCA_GC
Cuts at: 2556 2580 2556
Size: 24 7387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3373 5303 6513 3373
Size: 1930 1210 4271
Fragments arranged by size:
4271 1930 1210
BclI T'GATC_A
Cuts at: 1548 3789 4434 1548
Size: 2241 645 4525
Fragments arranged by size:
4525 2241 645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 3767 4296
Size: 107 122 71 631 605 365 1730 529
Cuts at: 4296 6022 136
Size: 1726 1525
Fragments arranged by size:
1730 1726 1525 631 605 529 365 122
107 71
BglII A'GATC_T
Cuts at: 3484 4651 7406 3484
Size: 1167 2755 3489
Fragments arranged by size:
3489 2755 1167
BlpI GC'TnA_GC
Cuts at: 4828 4828
Size: 7411
Bme1580I G_kGCm'C
Cuts at: 1790 2296 2772 5472 6628 1790
Size: 506 476 2700 1156 2573
Fragments arranged by size:
2700 2573 1156 506 476
BmgBI CAC'GTC
Cuts at: 1318 4606 1318
Size: 3288 4123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 4034 5142
Size: 416 53 83 399 24 1233 1384 1108
Cuts at: 5142 6100 442
Size: 958 1753
Fragments arranged by size:
1753 1384 1233 1108 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2906 3041 3137 4547 6071 1239
Size: 1667 135 96 1410 1524 2579
Fragments arranged by size:
2579 1667 1524 1410 135 96
Bpu10I CC'TnA_GC
Cuts at: 2556 2580 3801 2556
Size: 24 1221 6166
Fragments arranged by size:
6166 1221 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 5536 6401 6699 2966
Size: 2570 865 298 3678
Fragments arranged by size:
3678 2570 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2430 3317 6074 882
Size: 617 931 887 2757 2219
Fragments arranged by size:
2757 2219 931 887 617
BsaAI yAC'GTr
Cuts at: 493 1204 3229 3571 4590 5202 493
Size: 711 2025 342 1019 612 2702
Fragments arranged by size:
2702 2025 1019 711 612 342
BsaBI GATnn'nnATC
Cuts at: 7405 7405
Size: 7411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3309 3384 5218 5600
Size: 53 83 186 936 1776 75 1834 382
Cuts at: 5600 6235 275
Size: 635 1451
Fragments arranged by size:
1834 1776 1451 936 635 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 2432 5844 6242 6516 6663 7074 2432
Size: 3412 398 274 147 411 2769
Fragments arranged by size:
3412 2769 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2145 2175 3197 3227 1178
Size: 30 165 30 742 30 1022 30 5362
Fragments arranged by size:
5362 1022 742 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3218 3521 4427 1186
Size: 2032 303 906 4170
Fragments arranged by size:
4170 2032 906 303
BseYI C'CCAG_C
Cuts at: 6614 7110 6614
Size: 496 6915
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 3235 4100 1163
Size: 573 819 680 865 4474
Fragments arranged by size:
4474 865 819 680 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5622 6650 1540
Size: 275 186 2860 761 1028 2301
Fragments arranged by size:
2860 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2469 2489 5472 5557 6628 2244
Size: 225 20 2983 85 1071 3027
Fragments arranged by size:
3027 2983 1071 225 85 20
BsmI GAATG_Cn'
Cuts at: 3855 4922 5015 3855
Size: 1067 93 6251
Fragments arranged by size:
6251 1067 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2772 2772
Size: 7411
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2296 2469 2489 2772 2803 3632
Size: 399 454 52 173 20 283 31 829
Cuts at: 3632 4730 5472 5557 6628 1391
Size: 1098 742 85 1071 2174
Fragments arranged by size:
2174 1098 1071 829 742 454 399 283
173 85 52 31 20
BspEI T'CCGG_A
Cuts at: 7074 7074
Size: 7411
BspHI T'CATG_A
Cuts at: 3978 4162 4431 5300 7030 3978
Size: 184 269 869 1730 4359
Fragments arranged by size:
4359 1730 869 269 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 4689 4766 844
Size: 1604 2241 77 3489
Fragments arranged by size:
3489 2241 1604 77
BsrBI CCG'CTC
Cuts at: 1394 1823 4613 5298 1394
Size: 429 2790 685 3507
Fragments arranged by size:
3507 2790 685 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4125 5906 6080 65
Size: 1183 2877 1781 174 1396
Fragments arranged by size:
2877 1781 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2158 2650 3462 3851 6055 6242 1700
Size: 110 348 492 812 389 2204 187 2869
Fragments arranged by size:
2869 2204 812 492 389 348 187 110
BsrGI T'GTAC_A
Cuts at: 96 2493 3138 3471 96
Size: 2397 645 333 4036
Fragments arranged by size:
4036 2397 645 333
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 5471 6483 5471
Size: 1012 6399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7411
BstBI TT'CG_AA
Cuts at: 4704 6215 4704
Size: 1511 5900
BstEII G'GTnAC_C
Cuts at: 1184 2818 3805 1184
Size: 1634 987 4790
Fragments arranged by size:
4790 1634 987
BstXI CCAn_nnnn'nTGG
Cuts at: 3019 3868 3964 3019
Size: 849 96 6466
Fragments arranged by size:
6466 849 96
BstYI r'GATC_y
Cuts at: 1972 2673 3003 3484 3621 3887 4651 4741 5108
Size: 701 330 481 137 266 764 90 367
Cuts at: 5108 5493 5510 6962 7048 7060 7406 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 764 701 481 385 367 346
330 266 137 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2060 2586 2060
Size: 526 6885
BtgI C'CryG_G
Cuts at: 513 1065 2277 2677 4240 4491 513
Size: 552 1212 400 1563 251 3433
Fragments arranged by size:
3433 1563 1212 552 400 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6672
Fragments arranged by size:
6672 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2327 2944 3246 3402 4927 5721 5741 2022
Size: 305 617 302 156 1525 794 20 3692
Fragments arranged by size:
3692 1525 794 617 305 302 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4480 4515 529
Size: 35 3916 35 3425
Fragments arranged by size:
3916 3425 35 35
DraI TTT'AAA
Cuts at: 4715 4715
Size: 7411
DraIII CAC_nnn'GTG
Cuts at: 2080 4270 2080
Size: 2190 5221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6418 809
Size: 507 5102 1802
Fragments arranged by size:
5102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2355 2807
Size: 54 1425 50 275 18 208 317 452
Cuts at: 2807 3399 3513 3904 4346 4608 4858 5747 7376
Size: 592 114 391 442 262 250 889 1629
Cuts at: 7376 8
Size: 43
Fragments arranged by size:
1629 1425 889 592 452 442 391 317
275 262 250 208 114 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3281 4185 5341 1353
Size: 1160 768 904 1156 3423
Fragments arranged by size:
3423 1160 1156 904 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2429 5998 6372 6518 387
Size: 1064 138 840 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 840 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2242
Size: 7411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2535 2742 2906 3041 3137 3581 4547
Size: 393 903 207 164 135 96 444 966
Cuts at: 4547 5474 6071 6858 7126 1239
Size: 927 597 787 268 1524
Fragments arranged by size:
1524 966 927 903 787 597 444 393
268 207 164 135 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2063 2589 3679 4070 4347 2063
Size: 526 1090 391 277 5127
Fragments arranged by size:
5127 1090 526 391 277
EcoO109I rG'GnC_Cy
Cuts at: 2349 3526 4339 4540 2349
Size: 1177 813 201 5220
Fragments arranged by size:
5220 1177 813 201
EcoRI G'AATT_C
Cuts at: 4720 5098 4720
Size: 378 7033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 7411
FspI TGC'GCA
Cuts at: 5917 5917
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 3312 6558 1678
Size: 1634 3246 2531
Fragments arranged by size:
3246 2531 1634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2823 2855 3316 3348 4426
Size: 32 135 32 1251 32 461 32 1078
Cuts at: 4426 4458 6052 6084 6126 6158 1373
Size: 32 1594 32 42 32 2626
Fragments arranged by size:
2626 1594 1251 1078 461 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3388 3874 4585 4755 5001 669
Size: 988 281 1450 486 711 170 246 3079
Fragments arranged by size:
3079 1450 988 711 486 281 246 170
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7411
HpaI GTT'AAC
Cuts at: 3874 5001 3874
Size: 1127 6284
KasI G'GCGC_C
Cuts at: 3308 3308
Size: 7411
KpnI G_GTAC'C
Cuts at: 4736 4736
Size: 7411
MfeI C'AATT_G
Cuts at: 5010 5010
Size: 7411
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6525 6709 1305
Size: 5220 184 2007
Fragments arranged by size:
5220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2040 3515 10
Size: 54 1425 343 208 1475 3906
Fragments arranged by size:
3906 1475 1425 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3785 3927 3962 4382 5369 5728
Size: 648 1110 1509 142 35 420 987 359
Cuts at: 5728 5887 518
Size: 159 2042
Fragments arranged by size:
2042 1509 1110 987 648 420 359 159
142 35
MspA1I CmG'CkG
Cuts at: 1606 2679 2884 3001 3172 3758 4222 4827 5504
Size: 1073 205 117 171 586 464 605 677
Cuts at: 5504 6652 6897 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1073 677 605 586 464 245
205 171 117
NaeI GCC'GGC
Cuts at: 2652 3464 3853 2652
Size: 812 389 6210
Fragments arranged by size:
6210 812 389
NarI GG'CG_CC
Cuts at: 3309 3309
Size: 7411
NcoI C'CATG_G
Cuts at: 513 1065 2277 513
Size: 552 1212 5647
Fragments arranged by size:
5647 1212 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NgoMIV G'CCGG_C
Cuts at: 2650 3462 3851 2650
Size: 812 389 6210
Fragments arranged by size:
6210 812 389
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 3265 3580 4399 4769 6314 1132
Size: 97 2036 315 819 370 1545 2229
Fragments arranged by size:
2229 2036 1545 819 370 315 97
PasI CC'CwG_GG
Cuts at: 1348 2128 1348
Size: 780 6631
PciI A'CATG_T
Cuts at: 4395 6310 4395
Size: 1915 5496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2230 2364 2409 3890 1108
Size: 972 150 134 45 1481 4629
Fragments arranged by size:
4629 1481 972 150 134 45
PfoI T'CCnGG_A
Cuts at: 2469 3018 3746 2469
Size: 549 728 6134
Fragments arranged by size:
6134 728 549
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PmlI CAC'GTG
Cuts at: 3571 3571
Size: 7411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5480 5512 6993 7025 5480
Size: 32 1481 32 5866
Fragments arranged by size:
5866 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4339 4339
Size: 7411
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7411
PsiI TTA'TAA
Cuts at: 4981 4981
Size: 7411
PstI C_TGCA'G
Cuts at: 830 2264 2451 4763 830
Size: 1434 187 2312 3478
Fragments arranged by size:
3478 2312 1434 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2884 3172 3758 2884
Size: 288 586 6537
Fragments arranged by size:
6537 586 288
SacI G_AGCT'C
Cuts at: 2244 2244
Size: 7411
SacII CC_GC'GG
Cuts at: 2680 2680
Size: 7411
SalI G'TCGA_C
Cuts at: 1936 3386 4753 1936
Size: 1450 1367 4594
Fragments arranged by size:
4594 1450 1367
SapI GCTCTTCn'nnn_
Cuts at: 3281 3281
Size: 7411
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 2171 4286 5659 7371 2171
Size: 2115 1373 1712 2211
Fragments arranged by size:
2211 2115 1712 1373
SfcI C'TryA_G
Cuts at: 826 1045 2260 2447 4759 5894 6575 6766 826
Size: 219 1215 187 2312 1135 681 191 1471
Fragments arranged by size:
2312 1471 1215 1135 681 219 191 187
SfoI GGC'GCC
Cuts at: 3310 3310
Size: 7411
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2945 5515 6416 6678 820
Size: 197 926 1002 2570 901 262 1553
Fragments arranged by size:
2570 1553 1002 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 3265 4769 1132
Size: 2133 1504 3774
Fragments arranged by size:
3774 2133 1504
SspI AAT'ATT
Cuts at: 5 52 2878 5335 5
Size: 47 2826 2457 2081
Fragments arranged by size:
2826 2457 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 2763 1517
Size: 1197 49 6165
Fragments arranged by size:
6165 1197 49
StyI C'CwwG_G
Cuts at: 513 1065 2277 2336 3408 4407 4850 513
Size: 552 1212 59 1072 999 443 3074
Fragments arranged by size:
3074 1212 1072 999 552 443 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 4616 5438 5455 5608 5793 6238
Size: 1255 495 2541 822 17 153 185 445
Cuts at: 6238 325
Size: 1498
Fragments arranged by size:
2541 1498 1255 822 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2493 3138
Size: 274 80 33 51 1443 192 324 645
Cuts at: 3138 3471 4284 5657 7369 96
Size: 333 813 1373 1712 138
Fragments arranged by size:
1712 1443 1373 813 645 333 324 274
192 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3430 3462 7371 7403 3197
Size: 32 201 32 3909 32 3205
Fragments arranged by size:
3909 3205 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7411
XbaI T'CTAG_A
Cuts at: 4747 4747
Size: 7411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3278 3526 3278
Size: 248 7163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7411
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 5540 5540
Size: 7411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3385 5219 276
Size: 53 83 186 936 1851 1834 2468
Fragments arranged by size:
2468 1851 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI