Restriction map for
pFN21AB4178
(Circular) (Six-base) MAPSORT of: pFN21AB4178.seq Check: 2641 from: 1 to: 5740
pFN21AB4178 5740 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3039 3867 4240 1563
Size: 1476 828 373 3063
Fragments arranged by size:
3063 1476 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 3803 5187 5455 1632
Size: 858 1313 1384 268 1917
Fragments arranged by size:
1917 1384 1313 858 268
AflII C'TTAA_G
Cuts at: 820 1017 2711 820
Size: 197 1694 3849
Fragments arranged by size:
3849 1694 197
AflIII A'CryG_T
Cuts at: 1203 2109 4639 5616 1203
Size: 906 2530 977 1327
Fragments arranged by size:
2530 1327 977 906
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaI G_GGCC'C
Cuts at: 2190 2190
Size: 5740
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2925 3049 3275 3427 5536 1493
Size: 1432 124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1432 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 3061 4516 618
Size: 325 217 627 384 890 1455 1842
Fragments arranged by size:
1842 1455 890 627 384 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2223 2349 2586 3059 1391
Size: 799 33 126 237 473 4072
Fragments arranged by size:
4072 799 473 237 126 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2574 928
Size: 971 129 546 4094
Fragments arranged by size:
4094 971 546 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3931 3965 1903
Size: 34 50 34 1910 34 3678
Fragments arranged by size:
3678 1910 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2359 2474 5735 2359
Size: 115 3261 2364
Fragments arranged by size:
3261 2364 115
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2190 3801 4957 1790
Size: 400 1611 1156 2573
Fragments arranged by size:
2573 1611 1156 400
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 4948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2185 2613 4400 1239
Size: 946 428 1787 2579
Fragments arranged by size:
2579 1787 946 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2612 2624 3865 4730 5028 2570
Size: 42 12 1241 865 298 3282
Fragments arranged by size:
3282 1241 865 298 42 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2475 2840 1186
Size: 1289 365 4086
Fragments arranged by size:
4086 1289 365
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 2156 3190 3951 4979 1540
Size: 275 186 90 65 1034 761 1028 2301
Fragments arranged by size:
2301 1034 1028 761 275 186 90 65
BsiHKAI G_wGCw'C
Cuts at: 2349 2586 2661 3801 3886 4957 2349
Size: 237 75 1140 85 1071 3132
Fragments arranged by size:
3132 1140 1071 237 85 75
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2223 2349 2586 2661 3059 3801
Size: 399 400 33 126 237 75 398 742
Cuts at: 3801 3886 4957 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 398 237 126
85 75 33
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 3095 844
Size: 1292 959 3489
Fragments arranged by size:
3489 1292 959
BsrBI CCG'CTC
Cuts at: 1394 1823 2161 3627 1394
Size: 429 338 1466 3507
Fragments arranged by size:
3507 1466 429 338
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 2151 2168 2793 4384 4571 1700
Size: 110 276 65 17 625 1591 187 2869
Fragments arranged by size:
2869 1591 625 276 187 110 65 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2359 2474 3070 3437 3822 3839 5291 5377
Size: 387 115 596 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 596 387 385 367 346 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 513
Size: 552 1188 4000
Fragments arranged by size:
4000 1188 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2030 2114 4747 809
Size: 507 714 84 2633 1802
Fragments arranged by size:
2633 1802 714 507 84
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2153 2795
Size: 54 1425 50 275 18 258 65 642
Cuts at: 2795 3187 4076 5705 8
Size: 392 889 1629 43
Fragments arranged by size:
1629 1425 889 642 392 275 258 65
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2088 2153 3187 1537
Size: 275 276 65 1034 4090
Fragments arranged by size:
4090 1034 276 275 65
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2205 2591 2644 2686 3670 1353
Size: 695 157 386 53 42 984 3423
Fragments arranged by size:
3423 984 695 386 157 53 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2347 2584 2347
Size: 237 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2490 2613 3803 4400 5187 5455
Size: 393 553 305 123 1190 597 787 268
Cuts at: 5455 1239
Size: 1524
Fragments arranged by size:
1524 1190 787 597 553 393 305 268
123
EcoO109I rG'GnC_Cy
Cuts at: 2186 2187 2186
Size: 1 5739
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FseI GG_CCGG'CC
Cuts at: 2797 2797
Size: 5740
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 2864 2896 4381
Size: 32 135 32 687 32 573 32 1485
Cuts at: 4381 4413 4455 4487 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1485 687 573 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 3084 3330 669
Size: 988 281 1084 62 246 3079
Fragments arranged by size:
3079 1084 988 281 246 62
HindIII A'AGCT_T
Cuts at: 748 2438 2588 748
Size: 1690 150 3900
Fragments arranged by size:
3900 1690 150
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3060 4854 5038 1305
Size: 1755 1794 184 2007
Fragments arranged by size:
2007 1794 1755 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4057 4216 518
Size: 648 2532 359 159 2042
Fragments arranged by size:
2532 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2255 3156 3833 4981 5226 1606
Size: 649 901 677 1148 245 2120
Fragments arranged by size:
2120 1148 901 677 649 245
NaeI GCC'GGC
Cuts at: 2088 2170 2795 2088
Size: 82 625 5033
Fragments arranged by size:
5033 625 82
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NgoMIV G'CCGG_C
Cuts at: 2086 2168 2793 2086
Size: 82 625 5033
Fragments arranged by size:
5033 625 82
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2016 2016
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PspOMI G'GGCC_C
Cuts at: 2186 2186
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2133 2791 3092 830
Size: 1303 658 301 3478
Fragments arranged by size:
3478 1303 658 301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SacI G_AGCT'C
Cuts at: 2349 2586 2349
Size: 237 5503
SacII CC_GC'GG
Cuts at: 2256 2256
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SapI GCTCTTCn'nnn_
Cuts at: 2205 2205
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2129 2787 3088 4223 4904 5095 826
Size: 219 1084 658 301 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 681 658 301 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 2591 2639 2711 3844 4745
Size: 197 926 642 6 48 72 1133 901
Cuts at: 4745 5007 820
Size: 262 1553
Fragments arranged by size:
1553 1133 926 901 642 262 197 72
48 6
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2865 3179 513
Size: 552 1800 314 3074
Fragments arranged by size:
3074 1800 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2020 3767 3784 3937 4122 4567 325
Size: 1255 440 1747 17 153 185 445 1498
Fragments arranged by size:
1747 1498 1255 445 440 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3986 5698 96
Size: 274 80 33 51 1443 2009 1712 138
Fragments arranged by size:
2009 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 2332 3076 2332
Size: 744 4996
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI