Restriction map for
pFN21AB4172
(Circular) (Six-base) MAPSORT of: pFN21AB4172.seq Check: 1370 from: 1 to: 5782
pFN21AB4172 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3125 1937
Size: 541 647 4594
Fragments arranged by size:
4594 647 541
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3845 5229 5497 1632
Size: 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2915 1166
Size: 1749 4033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2380 3091 3317 3469 5578 1493
Size: 887 711 226 152 2109 1697
Fragments arranged by size:
2109 1697 887 711 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2802 4336 160
Size: 872 1770 1534 1606
Fragments arranged by size:
1770 1606 1534 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2582 2814 3103 4558 618
Size: 325 217 627 795 232 289 1455 1842
Fragments arranged by size:
1842 1455 795 627 325 289 232 217
BanII G_rGCy'C
Cuts at: 1391 3101 1391
Size: 1710 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 928
Size: 971 216 4595
Fragments arranged by size:
4595 971 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2691 2885 3014 3674 4884 2691
Size: 194 129 660 1210 3589
Fragments arranged by size:
3589 1210 660 194 129
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2565 4393 136
Size: 107 122 71 631 605 893 1828 1525
Fragments arranged by size:
1828 1525 893 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2697 3199 2697
Size: 502 5280
Bme1580I G_kGCm'C
Cuts at: 1790 2817 3843 4999 1790
Size: 1027 1026 1156 2573
Fragments arranged by size:
2573 1156 1027 1026
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2696 3012 1056
Size: 1640 316 3826
Fragments arranged by size:
3826 1640 316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2778 2778
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4442 1239
Size: 3203 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3055 4445 882
Size: 617 1556 1390 2219
Fragments arranged by size:
2219 1556 1390 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2176 2206 3024 3054 1178
Size: 30 165 30 773 30 818 30 3906
Fragments arranged by size:
3906 818 773 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5782
BseYI C'CCAG_C
Cuts at: 2652 4985 5481 2652
Size: 2333 496 2953
Fragments arranged by size:
2953 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2817 3101 3843 3928 4999 1391
Size: 399 1027 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 1027 742 399 284 85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3037 3137 844
Size: 2193 100 3489
Fragments arranged by size:
3489 2193 100
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 2844 96
Size: 2748 3034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2489 2489
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2586 2997 513
Size: 552 939 582 411 3298
Fragments arranged by size:
3298 939 582 552 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2566 3229 4118
Size: 54 1425 50 275 18 736 663 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 736 663 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3845 4442 5229 5497 1239
Size: 393 2213 597 787 268 1524
Fragments arranged by size:
2213 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2823 2823
Size: 5782
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2194 2379 2140
Size: 54 185 5543
Fragments arranged by size:
5543 185 54
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2134 2479 3126 3372 669
Size: 988 281 154 42 345 647 246 3079
Fragments arranged by size:
3079 988 647 345 281 246 154 42
HindIII A'AGCT_T
Cuts at: 748 2210 748
Size: 1462 4320
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2081 2406 2700 4896 5080 1305
Size: 776 325 294 2196 184 2007
Fragments arranged by size:
2196 2007 776 325 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2568 10
Size: 54 1425 343 736 3224
Fragments arranged by size:
3224 1425 736 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2915 3740 4099 4258 518
Size: 648 1749 825 359 159 2042
Fragments arranged by size:
2042 1749 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2394 2656 3198 3875 5023 5268 1606
Size: 788 262 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 677 542 262 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2997 513
Size: 552 939 993 3298
Fragments arranged by size:
3298 993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 2692 3008 1052
Size: 1640 316 3826
Fragments arranged by size:
3826 1640 316
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2810 1348
Size: 1462 4320
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PfoI T'CCnGG_A
Cuts at: 2683 2683
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3048 3134 830
Size: 2218 86 3478
Fragments arranged by size:
3478 2218 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2656 2656
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 2477 3124 1936
Size: 541 647 4594
Fragments arranged by size:
4594 647 541
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 3042 4030 5742 3042
Size: 988 1712 3082
Fragments arranged by size:
3082 1712 988
SexAI A'CCwGG_T
Cuts at: 2481 2481
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 3044 3130 4265 4946 5137 826
Size: 219 1999 86 1135 681 191 1471
Fragments arranged by size:
1999 1471 1135 681 219 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2565 2565
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2559 1517
Size: 1042 4740
StyI C'CwwG_G
Cuts at: 513 1065 2004 2274 2997 3221 513
Size: 552 939 270 723 224 3074
Fragments arranged by size:
3074 939 723 552 270 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 3040 4028
Size: 274 80 33 51 1443 867 196 988
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 988 867 274 196 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 5742 5774 2176
Size: 32 3534 32 2184
Fragments arranged by size:
3534 2184 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4615
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfoI SrfI
SwaI XcmI