Restriction map for
pFN21AB4163
(Circular) (Six-base) MAPSORT of: pFN21AB4163.seq Check: 1504 from: 1 to: 7522
pFN21AB4163 7522 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5332 278
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 3723 4865 1937
Size: 274 1512 1142 4594
Fragments arranged by size:
4594 1512 1142 274
Acc65I G'GTAC_C
Cuts at: 4843 4843
Size: 7522
AclI AA'CG_TT
Cuts at: 1563 4821 5649 6022 1563
Size: 3258 828 373 3063
Fragments arranged by size:
3258 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2219 2414 2975 3672 4441 4703 4778
Size: 446 141 195 561 697 769 262 75
Cuts at: 4778 5585 6969 7237 1632
Size: 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 769 697 561 446 268
262 195 141 75
AfeI AGC'GCT
Cuts at: 2553 3173 2553
Size: 620 6902
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7325
AflIII A'CryG_T
Cuts at: 1203 4788 6421 7398 1203
Size: 3585 1633 977 1327
Fragments arranged by size:
3585 1633 1327 977
AgeI A'CCGG_T
Cuts at: 6353 6353
Size: 7522
AhdI GACnn_n'nnGTC
Cuts at: 6251 6251
Size: 7522
AleI CACnn'nnGTG
Cuts at: 1166 2276 1166
Size: 1110 6412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2449 2606 4628 4721 1184
Size: 1265 157 2022 93 3985
Fragments arranged by size:
3985 2022 1265 157 93
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 7490
AlwNI CAG_nnn'CTG
Cuts at: 2429 3457 3508 4578 4598 4681 5265 6837 2429
Size: 1028 51 1070 20 83 584 1572 3114
Fragments arranged by size:
3114 1572 1070 1028 584 83 51 20
ApaLI G'TGCA_C
Cuts at: 2644 3770 5579 6735 2644
Size: 1126 1809 1156 3431
Fragments arranged by size:
3431 1809 1156 1126
ApoI r'AATT_y
Cuts at: 1493 4831 5057 5209 7318 1493
Size: 3338 226 152 2109 1697
Fragments arranged by size:
3338 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6076 160
Size: 872 5044 1606
Fragments arranged by size:
5044 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
AvaI C'yCGr_G
Cuts at: 1870 1943 3336 3413 3799 4847 1870
Size: 73 1393 77 386 1048 4545
Fragments arranged by size:
4545 1393 1048 386 77 73
BamHI G'GATC_C
Cuts at: 2404 4722 4852 5219 2404
Size: 2318 130 367 4707
Fragments arranged by size:
4707 2318 367 130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3585 4010 4024 4112 4190
Size: 325 217 627 1798 425 14 88 78
Cuts at: 4190 4843 6298 618
Size: 653 1455 1842
Fragments arranged by size:
1842 1798 1455 653 627 425 325 217
88 78 14
BanII G_rGCy'C
Cuts at: 1391 2070 2317 3675 3821 4214 4388 4841 1391
Size: 679 247 1358 146 393 174 453 4072
Fragments arranged by size:
4072 1358 679 453 393 247 174 146
BbeI G_GCGC'C
Cuts at: 3589 3589
Size: 7522
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4441 928
Size: 971 2542 4009
Fragments arranged by size:
4009 2542 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2575 2609 3172 3206 4000 4034 5713
Size: 34 638 34 563 34 794 34 1679
Cuts at: 5713 5747 1903
Size: 34 3678
Fragments arranged by size:
3678 1679 794 638 563 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5414 6624 5414
Size: 1210 6312
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 3047 4166
Size: 107 122 71 631 605 589 786 1119
Cuts at: 4166 4209 6133 136
Size: 43 1924 1525
Fragments arranged by size:
1924 1525 1119 786 631 605 589 122
107 71 43
BglII A'GATC_T
Cuts at: 2046 2661 4182 4359 7517 2046
Size: 615 1521 177 3158 2051
Fragments arranged by size:
3158 2051 1521 615 177
BlpI GC'TnA_GC
Cuts at: 4939 4939
Size: 7522
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2648 3774 4013 4115 5583 6739 1790
Size: 362 496 1126 239 102 1468 1156 2573
Fragments arranged by size:
2573 1468 1156 1126 496 362 239 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 3917 4555
Size: 416 53 83 399 24 2315 185 638
Cuts at: 4555 5253 6211 442
Size: 698 958 1753
Fragments arranged by size:
2315 1753 958 698 638 416 399 185
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 3032 3422 3858 4199 4304 4403 6182
Size: 974 819 390 436 341 105 99 1779
Cuts at: 6182 1239
Size: 2579
Fragments arranged by size:
2579 1779 974 819 436 390 341 105
99
Bpu10I CC'TnA_GC
Cuts at: 4170 4170
Size: 7522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 4799 5647 6512 6810 2831
Size: 1968 848 865 298 3543
Fragments arranged by size:
3543 1968 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3491 4799 6185 882
Size: 617 1992 1308 1386 2219
Fragments arranged by size:
2219 1992 1386 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 3004 4789 5313 493
Size: 711 1800 1785 524 2702
Fragments arranged by size:
2702 1800 1785 711 524
BsaBI GATnn'nnATC
Cuts at: 2666 7516 2666
Size: 4850 2672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3765 5329 5711
Size: 53 83 186 936 2053 179 1564 382
Cuts at: 5711 6346 275
Size: 635 1451
Fragments arranged by size:
2053 1564 1451 936 635 382 186 179
83 53
BsaWI w'CCGG_w
Cuts at: 2382 5955 6353 6627 6774 7185 2382
Size: 3573 398 274 147 411 2719
Fragments arranged by size:
3573 2719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 2798 2828 3611
Size: 30 165 30 1092 30 273 30 783
Cuts at: 3611 3641 1178
Size: 30 5059
Fragments arranged by size:
5059 1092 783 273 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3890 3940 1186
Size: 2704 50 4768
Fragments arranged by size:
4768 2704 50
BseYI C'CCAG_C
Cuts at: 2463 2579 3889 4156 4739 4782 6725 7221 2463
Size: 116 1310 267 583 43 1943 496 2764
Fragments arranged by size:
2764 1943 1310 583 496 267 116 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 3533 3977 4460 4729 1163
Size: 573 1218 579 444 483 269 3956
Fragments arranged by size:
3956 1218 579 573 483 444 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 4972 5733 6761 1540
Size: 275 186 706 2265 761 1028 2301
Fragments arranged by size:
2301 2265 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2648 2775 3185 3437 3774 3844 4063 4771
Size: 578 127 410 252 337 70 219 708
Cuts at: 4771 5583 5668 6739 2070
Size: 812 85 1071 2853
Fragments arranged by size:
2853 1071 812 708 578 410 337 252
219 127 85 70
BsmI GAATG_Cn'
Cuts at: 5033 5126 5033
Size: 93 7429
BsmBI CGTCTCn'nnnn_
Cuts at: 3650 3650
Size: 7522
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2152 2317 2648 2775 3185 3437
Size: 399 280 82 165 331 127 410 252
Cuts at: 3437 3675 3774 3821 3844 4013 4063 4115 4214
Size: 238 99 47 23 169 50 52 99
Cuts at: 4214 4388 4771 4841 5583 5668 6739 1391
Size: 174 383 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 383 331 280
252 238 174 169 165 127 99 99
85 82 70 52 50 47 23
BspEI T'CCGG_A
Cuts at: 2382 7185 2382
Size: 4803 2719
BspHI T'CATG_A
Cuts at: 3027 4257 4392 5411 7141 3027
Size: 1230 135 1019 1730 3408
Fragments arranged by size:
3408 1730 1230 1019 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 3508 3567 3894 4236 4243 4877 844
Size: 1754 910 59 327 342 7 634 3489
Fragments arranged by size:
3489 1754 910 634 342 327 59 7
BsrBI CCG'CTC
Cuts at: 1394 1823 2731 3930 5409 1394
Size: 429 908 1199 1479 3507
Fragments arranged by size:
3507 1479 1199 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6017 6191 65
Size: 1183 4769 174 1396
Fragments arranged by size:
4769 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4509 6166 6353 1700
Size: 110 2699 1657 187 2869
Fragments arranged by size:
2869 2699 1657 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7522
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7522
BssSI C'ACGA_G
Cuts at: 5582 6594 5582
Size: 1012 6510
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4598 794
Size: 3804 3718
BstBI TT'CG_AA
Cuts at: 6326 6326
Size: 7522
BstEII G'GTnAC_C
Cuts at: 1184 2734 3893 1184
Size: 1550 1159 4813
Fragments arranged by size:
4813 1550 1159
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2545 3154 3622 4021 4108 4222 2533
Size: 12 609 468 399 87 114 5833
Fragments arranged by size:
5833 609 468 399 114 87 12
BstYI r'GATC_y
Cuts at: 1972 2046 2404 2661 2949 4182 4359 4722 4852
Size: 74 358 257 288 1233 177 363 130
Cuts at: 4852 5219 5604 5621 7073 7159 7171 7517 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1233 385 367 363 358 346
288 257 177 130 86 74 17 12
BstZ17I GTA'TAC
Cuts at: 2212 2212
Size: 7522
Bsu36I CC'TnA_GG
Cuts at: 3330 3330
Size: 7522
BtgI C'CryG_G
Cuts at: 513 1065 2130 2331 2526 3568 3597 4221 513
Size: 552 1065 201 195 1042 29 624 3814
Fragments arranged by size:
3814 1065 1042 624 552 201 195 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 487
Size: 683 56 1814 4969
Fragments arranged by size:
4969 1814 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2328 2550 2840 2873 4078 4472 5038 5832
Size: 219 222 290 33 1205 394 566 794
Cuts at: 5832 5852 2109
Size: 20 3779
Fragments arranged by size:
3779 1205 794 566 394 290 222 219
33 20
ClaI AT'CG_AT
Cuts at: 1554 2123 4443 1554
Size: 569 2320 4633
Fragments arranged by size:
4633 2320 569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3078 3113 529
Size: 35 2514 35 4938
Fragments arranged by size:
4938 2514 35 35
DraI TTT'AAA
Cuts at: 4826 4826
Size: 7522
DraIII CAC_nnn'GTG
Cuts at: 2652 3300 3302 2652
Size: 648 2 6872
Fragments arranged by size:
6872 648 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6529 809
Size: 507 5213 1802
Fragments arranged by size:
5213 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2262 2439
Size: 54 1425 50 275 18 423 9 177
Cuts at: 2439 2535 3136 3531 3942 4425 4524 4969 5858
Size: 96 601 395 411 483 99 445 889
Cuts at: 5858 7487 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 601 483 445 423 411
395 275 177 99 96 54 50 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4969 1537
Size: 275 3157 4090
Fragments arranged by size:
4090 3157 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 3648 5452 1353
Size: 806 1489 1804 3423
Fragments arranged by size:
3423 1804 1489 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3023 4727 6109 6483 6629 387
Size: 1064 138 1434 1704 1382 374 146 1280
Fragments arranged by size:
1704 1434 1382 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 7522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2213 2219 2414 2975 3032 3422
Size: 393 446 135 6 195 561 57 390
Cuts at: 3422 3672 3858 4199 4304 4403 4441 4703 4778
Size: 250 186 341 105 99 38 262 75
Cuts at: 4778 5585 6182 6969 7237 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 561 446 393 390
341 268 262 250 195 186 135 105
99 75 57 38 6
EcoO109I rG'GnC_Cy
Cuts at: 2293 3520 2293
Size: 1227 6295
EcoRI G'AATT_C
Cuts at: 4831 5209 4831
Size: 378 7144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 3271 2992
Size: 279 7243
FspI TGC'GCA
Cuts at: 2417 2915 6028 2417
Size: 498 3113 3911
Fragments arranged by size:
3911 3113 498
HaeII r_GCGC'y
Cuts at: 1678 2555 3175 3589 6669 1678
Size: 877 620 414 3080 2531
Fragments arranged by size:
3080 2531 877 620 414
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 2798 2830 4050
Size: 32 135 32 923 32 271 32 1220
Cuts at: 4050 4082 4390 4422 6163 6195 6237 6269 1373
Size: 32 308 32 1741 32 42 32 2626
Fragments arranged by size:
2626 1741 1220 923 308 271 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2269 2350 3214 4866 5112
Size: 988 281 232 99 81 864 1652 246
Cuts at: 5112 669
Size: 3079
Fragments arranged by size:
3079 1652 988 864 281 246 232 99
81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7522
HpaI GTT'AAC
Cuts at: 5112 5112
Size: 7522
KasI G'GCGC_C
Cuts at: 3585 3585
Size: 7522
KpnI G_GTAC'C
Cuts at: 4847 4847
Size: 7522
MfeI C'AATT_G
Cuts at: 5121 5121
Size: 7522
MluI A'CGCG_T
Cuts at: 7398 7398
Size: 7522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 4055 6636 6820 1305
Size: 1233 1517 2581 184 2007
Fragments arranged by size:
2581 2007 1517 1233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 2441 2537 3533 3944
Size: 54 1425 343 423 186 96 996 411
Cuts at: 3944 4526 10
Size: 582 3006
Fragments arranged by size:
3006 1425 996 582 423 411 343 186
96 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2531 2807 3152 4220 4397 4703
Size: 648 1110 255 276 345 1068 177 306
Cuts at: 4703 5480 5839 5998 518
Size: 777 359 159 2042
Fragments arranged by size:
2042 1110 1068 777 648 359 345 306
276 255 177 159
MspA1I CmG'CkG
Cuts at: 1606 2429 2467 2765 3141 3448 3454 4234 4743
Size: 823 38 298 376 307 6 780 509
Cuts at: 4743 4938 5615 6763 7008 1606
Size: 195 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 780 677 509 376 307
298 245 195 38 6
NarI GG'CG_CC
Cuts at: 3586 3586
Size: 7522
NcoI C'CATG_G
Cuts at: 513 1065 2331 2526 4221 513
Size: 552 1266 195 1695 3814
Fragments arranged by size:
3814 1695 1266 552 195
NdeI CA'TA_TG
Cuts at: 387 2410 4129 387
Size: 2023 1719 3780
Fragments arranged by size:
3780 2023 1719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7522
NotI GC'GGCC_GC
Cuts at: 4969 4969
Size: 7522
NspI r_CATG'y
Cuts at: 1132 1229 2179 4669 4880 6425 1132
Size: 97 950 2490 211 1545 2229
Fragments arranged by size:
2490 2229 1545 950 211 97
PasI CC'CwG_GG
Cuts at: 1348 3877 1348
Size: 2529 4993
PciI A'CATG_T
Cuts at: 6421 6421
Size: 7522
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 3067 3351 3508 4354 1108
Size: 1362 597 284 157 846 4276
Fragments arranged by size:
4276 1362 846 597 284 157
PfoI T'CCnGG_A
Cuts at: 2487 2670 3225 2487
Size: 183 555 6784
Fragments arranged by size:
6784 555 183
PmeI GTTT'AAAC
Cuts at: 4826 4826
Size: 7522
PmlI CAC'GTG
Cuts at: 4789 4789
Size: 7522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 5591 5623 7104 7136 4547
Size: 32 1012 32 1481 32 4933
Fragments arranged by size:
4933 1481 1012 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 3520 2293
Size: 1227 6295
PshAI GACnn'nnGTC
Cuts at: 1613 3209 1613
Size: 1596 5926
PsiI TTA'TAA
Cuts at: 5092 5092
Size: 7522
PspXI vC'TCGA_Gb
Cuts at: 3413 3799 3413
Size: 386 7136
PstI C_TGCA'G
Cuts at: 830 2901 3453 4233 4617 4874 830
Size: 2071 552 780 384 257 3478
Fragments arranged by size:
3478 2071 780 552 384 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7522
PvuII CAG'CTG
Cuts at: 2429 2467 3448 3454 4234 4743 2429
Size: 38 981 6 780 509 5208
Fragments arranged by size:
5208 981 780 509 38 6
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 7522
SalI G'TCGA_C
Cuts at: 1936 4864 1936
Size: 2928 4594
SbfI CC_TGCA'GG
Cuts at: 4874 4874
Size: 7522
ScaI AGT'ACT
Cuts at: 5770 7482 5770
Size: 1712 5810
SfcI C'TryA_G
Cuts at: 826 1045 2897 3449 4229 4613 4870 6005 6686
Size: 219 1852 552 780 384 257 1135 681
Cuts at: 6686 6877 826
Size: 191 1471
Fragments arranged by size:
1852 1471 1135 780 681 552 384 257
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2261 3047 2261
Size: 786 6736
SfoI GGC'GCC
Cuts at: 3587 3587
Size: 7522
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7522
SmaI CCC'GGG
Cuts at: 1872 3338 4849 1872
Size: 1466 1511 4545
Fragments arranged by size:
4545 1511 1466
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3413 3799 4814 5626 6527
Size: 197 926 903 567 386 1015 812 901
Cuts at: 6527 6789 820
Size: 262 1553
Fragments arranged by size:
1553 1015 926 903 901 812 567 386
262 197
SnaBI TAC'GTA
Cuts at: 493 5313 493
Size: 4820 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7522
SphI G_CATG'C
Cuts at: 1132 2179 4880 1132
Size: 1047 2701 3774
Fragments arranged by size:
3774 2701 1047
SrfI GCCC'GGGC
Cuts at: 3338 3338
Size: 7522
SspI AAT'ATT
Cuts at: 5 52 2021 5446 5
Size: 47 1969 3425 2081
Fragments arranged by size:
3425 2081 1969 47
StuI AGG'CCT
Cuts at: 1517 2944 4247 4799 1517
Size: 1427 1303 552 4240
Fragments arranged by size:
4240 1427 1303 552
StyI C'CwwG_G
Cuts at: 513 1065 2331 2526 2697 3207 3615 4221 4961
Size: 552 1266 195 171 510 408 606 740
Cuts at: 4961 513
Size: 3074
Fragments arranged by size:
3074 1266 740 606 552 510 408 195
171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3881 4078 4277 4378 4693 5549 5566
Size: 1255 2301 197 199 101 315 856 17
Cuts at: 5566 5719 5904 6349 325
Size: 153 185 445 1498
Fragments arranged by size:
2301 1498 1255 856 445 315 199 197
185 153 101 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 5768 7480
Size: 274 80 33 51 1443 1008 2783 1712
Cuts at: 7480 96
Size: 138
Fragments arranged by size:
2783 1712 1443 1008 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4116 4148 4464 4496 7482 7514 4116
Size: 32 316 32 2986 32 4124
Fragments arranged by size:
4124 2986 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7522
XbaI T'CTAG_A
Cuts at: 4858 4858
Size: 7522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 3604 3745 4351 4648 2764
Size: 840 141 606 297 5638
Fragments arranged by size:
5638 840 606 297 141
XhoI C'TCGA_G
Cuts at: 1943 3413 3799 1943
Size: 1470 386 5666
Fragments arranged by size:
5666 1470 386
XmaI C'CCGG_G
Cuts at: 1870 3336 4847 1870
Size: 1466 1511 4545
Fragments arranged by size:
4545 1511 1466
XmnI GAAnn'nnTTC
Cuts at: 2972 5651 2972
Size: 2679 4843
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5330 276
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SwaI