Restriction map for
pFN21AB4161
(Circular) (Six-base) MAPSORT of: pFN21AB4161.seq Check: 4543 from: 1 to: 6118
pFN21AB4161 6118 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2872 2872
Size: 6118
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2219 3461 1937
Size: 282 1242 4594
Fragments arranged by size:
4594 1242 282
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4181 5565 5833 1632
Size: 2549 1384 268 1917
Fragments arranged by size:
2549 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3191 820
Size: 197 2174 3747
Fragments arranged by size:
3747 2174 197
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 2051
Size: 32 6086
AlwNI CAG_nnn'CTG
Cuts at: 2296 2497 3074 3861 5433 2296
Size: 201 577 787 1572 2981
Fragments arranged by size:
2981 1572 787 577 201
ApaI G_GGCC'C
Cuts at: 2572 2984 2572
Size: 412 5706
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2345 3011 3427 3653 3805 5914 1493
Size: 852 666 416 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 666 416 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2577 3147 3273 3297 3443 1870
Size: 73 634 570 126 24 146 4545
Fragments arranged by size:
4545 634 570 146 126 73 24
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3236 3315 3411 3439 4894
Size: 325 217 627 1449 79 96 28 1455
Cuts at: 4894 618
Size: 1842
Fragments arranged by size:
1842 1455 1449 627 325 217 96 79
28
BanII G_rGCy'C
Cuts at: 1391 2572 2984 3251 3437 1391
Size: 1181 412 267 186 4072
Fragments arranged by size:
4072 1181 412 267 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3194 928
Size: 971 1295 3852
Fragments arranged by size:
3852 1295 971
BbvCI CC'TCA_GC
Cuts at: 2388 2388
Size: 6118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2795 4010 5220 2795
Size: 1215 1210 3693
Fragments arranged by size:
3693 1215 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2572 2984 4179 5335 1790
Size: 782 412 1195 1156 2573
Fragments arranged by size:
2573 1195 1156 782 412
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 4963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 3849 4807
Size: 416 53 83 399 24 1238 1194 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1753 1238 1194 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3096 4778 1239
Size: 1857 1682 2579
Fragments arranged by size:
2579 1857 1682
Bpu10I CC'TnA_GC
Cuts at: 2388 2388
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2737 4243 5108 5406 2737
Size: 1506 865 298 3449
Fragments arranged by size:
3449 1506 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 1178
Size: 30 165 30 1048 30 4815
Fragments arranged by size:
4815 1048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2151 2740 2760 2763 2766 2805 2817
Size: 874 91 589 20 3 3 39 12
Cuts at: 2817 3175 1186
Size: 358 4129
Fragments arranged by size:
4129 874 589 358 91 39 20 12
3 3
BseYI C'CCAG_C
Cuts at: 5321 5817 5321
Size: 496 5622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3105 1163
Size: 573 1369 4176
Fragments arranged by size:
4176 1369 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3251 4179 4264 5335 3251
Size: 928 85 1071 4034
Fragments arranged by size:
4034 1071 928 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
BsmBI CGTCTCn'nnnn_
Cuts at: 3104 3104
Size: 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2572 2984 3251 3437 4179 4264 5335
Size: 399 782 412 267 186 742 85 1071
Cuts at: 5335 1391
Size: 2174
Fragments arranged by size:
2174 1071 782 742 412 399 267 186
85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 3024 4007 5737 3024
Size: 983 1730 3405
Fragments arranged by size:
3405 1730 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2872 3473 844
Size: 2028 601 3489
Fragments arranged by size:
3489 2028 601
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3126 4613 4787 65
Size: 1183 1878 1487 174 1396
Fragments arranged by size:
1878 1487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2479 3291 3408 4762 4949 1700
Size: 110 669 812 117 1354 187 2869
Fragments arranged by size:
2869 1354 812 669 187 117 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 2643 4178 5190 2643
Size: 1535 1012 3571
Fragments arranged by size:
3571 1535 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2803 794
Size: 2009 4109
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 3282 1184
Size: 2098 4020
BstYI r'GATC_y
Cuts at: 1972 3322 3448 3815 4200 4217 5669 5755 5767
Size: 1350 126 367 385 17 1452 86 12
Cuts at: 5767 6113 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 126 86
17 12
BstZ17I GTA'TAC
Cuts at: 2220 2220
Size: 6118
BtgI C'CryG_G
Cuts at: 513 1065 2564 2962 513
Size: 552 1499 398 3669
Fragments arranged by size:
3669 1499 552 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3251 487
Size: 683 56 2025 3354
Fragments arranged by size:
3354 2025 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 3634 4428 4448 3058
Size: 576 794 20 4728
Fragments arranged by size:
4728 794 576 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2368 2481 3565
Size: 54 1425 50 275 18 538 113 1084
Cuts at: 3565 4454 6083 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1084 889 538 275 113 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 4048 1353
Size: 1903 792 3423
Fragments arranged by size:
3423 1903 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3249 3249
Size: 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3096 4181 4778 5565 5833 1239
Size: 393 1464 1085 597 787 268 1524
Fragments arranged by size:
1524 1464 1085 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2059 2764 2915 2981 2059
Size: 705 151 66 5196
Fragments arranged by size:
5196 705 151 66
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
EcoRV GAT'ATC
Cuts at: 3022 3022
Size: 6118
FseI GG_CCGG'CC
Cuts at: 2483 2483
Size: 6118
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373
Size: 32 135 32 3187 32 42 32 2626
Fragments arranged by size:
3187 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2755 748
Size: 2007 4111
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3105 3717 3105
Size: 612 5506
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 5232 5416 1305
Size: 1104 2823 184 2007
Fragments arranged by size:
2823 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2370 10
Size: 54 1425 343 538 3758
Fragments arranged by size:
3758 1425 538 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4076 4435 4594 518
Size: 648 2910 359 159 2042
Fragments arranged by size:
2910 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2296 3534 4211 5359 5604 1606
Size: 690 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 690 677 245
NaeI GCC'GGC
Cuts at: 2481 2481
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 2962 513
Size: 552 1897 3669
Fragments arranged by size:
3669 1897 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NgoMIV G'CCGG_C
Cuts at: 2479 2479
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2849 1348
Size: 1501 4617
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PfoI T'CCnGG_A
Cuts at: 3254 3254
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2764 2059
Size: 705 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2568 2980 2568
Size: 412 5706
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2296 2296
Size: 6118
SacI G_AGCT'C
Cuts at: 3251 3251
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SanDI GG'GwC_CC
Cuts at: 2764 2764
Size: 6118
SapI GCTCTTCn'nnn_
Cuts at: 3256 3256
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2224 2316 2907 2994 3226 3466 4601
Size: 219 1179 92 591 87 232 240 1135
Cuts at: 4601 5282 5473 826
Size: 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 591 240 232 219
191 92 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3149 3275 3299 3445 1872
Size: 1277 126 24 146 4545
Fragments arranged by size:
4545 1277 146 126 24
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3191 4222 5123 5385 820
Size: 197 926 809 439 1031 901 262 1553
Fragments arranged by size:
1553 1031 926 901 809 439 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2942 1517
Size: 1425 4693
StyI C'CwwG_G
Cuts at: 513 1065 2962 3557 513
Size: 552 1897 595 3074
Fragments arranged by size:
3074 1897 595 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3224 4145 4162 4315 4500 4945 325
Size: 1255 1644 921 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1255 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4364 6076 96
Size: 274 80 33 51 1443 2387 1712 138
Fragments arranged by size:
2387 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 6078 6110 2451
Size: 32 3595 32 2459
Fragments arranged by size:
3595 2459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3147 3273 3297 3443 1870
Size: 1277 126 24 146 4545
Fragments arranged by size:
4545 1277 146 126 24
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstXI Bsu36I DraIII EcoNI FalI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI