Restriction map for
pFN21AB4148
(Circular) (Six-base) MAPSORT of: pFN21AB4148.seq Check: 4184 from: 1 to: 6277
pFN21AB4148 6277 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2362 2704 2362
Size: 342 5935
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2973 3620 1937
Size: 1036 647 4594
Fragments arranged by size:
4594 1036 647
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 2189 2459 2760 3189 4340 5724 5992
Size: 513 44 270 301 429 1151 1384 268
Cuts at: 5992 1632
Size: 1917
Fragments arranged by size:
1917 1384 1151 513 429 301 270 268
44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 3361 4020 5592 3361
Size: 659 1572 4046
Fragments arranged by size:
4046 1572 659
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2831 3228 3586 3812 3964 6073 1493
Size: 1338 397 358 226 152 2109 1697
Fragments arranged by size:
2109 1697 1338 397 358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2936 4831 160
Size: 872 1904 1895 1606
Fragments arranged by size:
1904 1895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2066 3602 1870
Size: 73 82 41 1536 4545
Fragments arranged by size:
4545 1536 82 73 41
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 3598 5053 618
Size: 325 217 627 1166 645 1455 1842
Fragments arranged by size:
1842 1455 1166 645 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2872 3596 1391
Size: 1481 724 4072
Fragments arranged by size:
4072 1481 724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3070 3183 3250 928
Size: 971 1171 113 67 3955
Fragments arranged by size:
3955 1171 971 113 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2142 4169 5379 2142
Size: 2027 1210 3040
Fragments arranged by size:
3040 2027 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 4338 5494 1790
Size: 2548 1156 2573
Fragments arranged by size:
2573 2548 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4008 4966 442
Size: 416 53 83 399 24 2591 958 1753
Fragments arranged by size:
2591 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3366 4937 1239
Size: 2127 1571 2579
Fragments arranged by size:
2579 2127 1571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2865 3284 4402 5267 5565 2865
Size: 419 1118 865 298 3577
Fragments arranged by size:
3577 1118 865 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 4068 493
Size: 711 1880 984 2702
Fragments arranged by size:
2702 1880 984 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4710 5108 5382 5529 5940 4710
Size: 398 274 147 411 5047
Fragments arranged by size:
5047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3096 3126 1178
Size: 30 165 30 1693 30 4329
Fragments arranged by size:
4329 1693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3290 1186
Size: 2104 4173
BseYI C'CCAG_C
Cuts at: 5480 5976 5480
Size: 496 5781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2016 2580 1163
Size: 573 280 564 4860
Fragments arranged by size:
4860 573 564 280
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2872 4338 4423 5494 2872
Size: 1466 85 1071 3655
Fragments arranged by size:
3655 1466 1071 85
BsmI GAATG_Cn'
Cuts at: 2492 3483 3788 3881 2492
Size: 991 305 93 4888
Fragments arranged by size:
4888 991 305 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2872 3596 4338 4423 5494 1391
Size: 399 1082 724 742 85 1071 2174
Fragments arranged by size:
2174 1082 1071 742 724 399 85
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 2872 4166 5896 2872
Size: 1294 1730 3253
Fragments arranged by size:
3253 1730 1294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2362 2704 3632 844
Size: 1518 342 928 3489
Fragments arranged by size:
3489 1518 928 342
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 4921 5108 1700
Size: 110 210 2901 187 2869
Fragments arranged by size:
2901 2869 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 2901 3499 96
Size: 2805 598 2874
Fragments arranged by size:
2874 2805 598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3361 794
Size: 2567 3710
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 3188 3188
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272
Size: 1635 367 385 17 1452 86 12 346
Cuts at: 6272 1972
Size: 1977
Fragments arranged by size:
1977 1635 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 3550 513
Size: 552 939 301 1245 3240
Fragments arranged by size:
3240 1245 939 552 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2096 487
Size: 683 56 870 4668
Fragments arranged by size:
4668 870 683 56
BtsI GCAGTG_nn'
Cuts at: 2829 3793 4587 4607 2829
Size: 964 794 20 4499
Fragments arranged by size:
4499 964 794 20
ClaI AT'CG_AT
Cuts at: 1554 2858 1554
Size: 1304 4973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 2604 3581 2604
Size: 977 5300
DraIII CAC_nnn'GTG
Cuts at: 2182 2292 2182
Size: 110 6167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2081 5284 809
Size: 507 765 3203 1802
Fragments arranged by size:
3203 1802 765 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2308 3724
Size: 54 1425 50 275 18 177 301 1416
Cuts at: 3724 4613 6242 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1416 889 301 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3438 4207 1353
Size: 2085 769 3423
Fragments arranged by size:
3423 2085 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2870 2870
Size: 6277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2189 2459 2760 3189 3366 4340
Size: 393 513 44 270 301 429 177 974
Cuts at: 4340 4937 5724 5992 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 974 787 597 513 429 393 301
270 268 177 44
EcoO109I rG'GnC_Cy
Cuts at: 3304 3304
Size: 6277
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 6277
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4918 4950 4992 5024 1373
Size: 32 135 32 3346 32 42 32 2626
Fragments arranged by size:
3346 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3621 3867 669
Size: 988 281 1683 246 3079
Fragments arranged by size:
3079 1683 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2559 2571 748
Size: 1811 12 4454
Fragments arranged by size:
4454 1811 12
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3237 3876 3237
Size: 639 5638
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3082 5391 5575 1305
Size: 1777 2309 184 2007
Fragments arranged by size:
2309 2007 1777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2310 10
Size: 54 1425 343 478 3977
Fragments arranged by size:
3977 1425 478 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 4235 4594 4753 518
Size: 648 2154 915 359 159 2042
Fragments arranged by size:
2154 2042 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3197 3693 4370 5518 5763 1606
Size: 1591 496 677 1148 245 2120
Fragments arranged by size:
2120 1591 1148 677 496 245
NaeI GCC'GGC
Cuts at: 2022 2022
Size: 6277
NcoI C'CATG_G
Cuts at: 513 1065 2004 2305 513
Size: 552 939 301 4485
Fragments arranged by size:
4485 939 552 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NgoMIV G'CCGG_C
Cuts at: 2020 2020
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 3539 3635 5180 1132
Size: 97 2310 96 1545 2229
Fragments arranged by size:
2310 2229 1545 97 96
PasI CC'CwG_GG
Cuts at: 1348 2154 1348
Size: 806 5471
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PmlI CAC'GTG
Cuts at: 3084 3084
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 2811 3847 2811
Size: 1036 5241
PstI C_TGCA'G
Cuts at: 830 3202 3437 3629 830
Size: 2372 235 192 3478
Fragments arranged by size:
3478 2372 235 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 3197 3197
Size: 6277
SacI G_AGCT'C
Cuts at: 2872 2872
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2893 2919 3198 3433 3625 4760 5441
Size: 219 1848 26 279 235 192 1135 681
Cuts at: 5441 5632 826
Size: 191 1471
Fragments arranged by size:
1848 1471 1135 681 279 235 219 192
191 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 2027 2068 3604 1872
Size: 155 41 1536 4545
Fragments arranged by size:
4545 1536 155 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2880 3299 4381 5282 5544 820
Size: 197 926 937 419 1082 901 262 1553
Fragments arranged by size:
1553 1082 937 926 901 419 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SrfI GCCC'GGGC
Cuts at: 2027 2027
Size: 6277
SspI AAT'ATT
Cuts at: 5 52 2507 3022 4201 5
Size: 47 2455 515 1179 2081
Fragments arranged by size:
2455 2081 1179 515 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2004 2305 3716 513
Size: 552 939 301 1411 3074
Fragments arranged by size:
3074 1411 939 552 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4304 4321 4474 4659 5104 325
Size: 1255 2724 17 153 185 445 1498
Fragments arranged by size:
2724 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3499 4523
Size: 274 80 33 51 1443 924 598 1024
Cuts at: 4523 6235 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1024 924 598 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3096 3128 6237 6269 3096
Size: 32 3109 32 3104
Fragments arranged by size:
3109 3104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 1316
Size: 762 5515
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3051 3051
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 2025 2066 3602 1870
Size: 155 41 1536 4545
Fragments arranged by size:
4545 1536 155 41
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SwaI