Restriction map for
pFN21AB4146
(Circular) (Six-base) MAPSORT of: pFN21AB4146.seq Check: 5589 from: 1 to: 6562
pFN21AB4146 6562 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3905 1937
Size: 1968 4594
Acc65I G'GTAC_C
Cuts at: 2609 3883 2609
Size: 1274 5288
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 2967 4625 6009 6277 1632
Size: 782 553 1658 1384 268 1917
Fragments arranged by size:
1917 1658 1384 782 553 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 5461 6438 1203
Size: 4258 977 1327
Fragments arranged by size:
4258 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 3099
Size: 32 6530
AlwNI CAG_nnn'CTG
Cuts at: 3242 4305 5877 3242
Size: 1063 1572 3927
Fragments arranged by size:
3927 1572 1063
ApaLI G'TGCA_C
Cuts at: 2352 2368 4619 5775 2352
Size: 16 2251 1156 3139
Fragments arranged by size:
3139 2251 1156 16
ApoI r'AATT_y
Cuts at: 1493 2291 3733 3871 4097 4249 6358 1493
Size: 798 1442 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 798 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 3887 1870
Size: 73 117 1827 4545
Fragments arranged by size:
4545 1827 117 73
BamHI G'GATC_C
Cuts at: 2805 3892 4259 2805
Size: 1087 367 5108
Fragments arranged by size:
5108 1087 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 3883 5338 618
Size: 325 217 627 822 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 822 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 3151 3881 1391
Size: 888 872 730 4072
Fragments arranged by size:
4072 888 872 730
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2755 928
Size: 971 180 676 4735
Fragments arranged by size:
4735 971 676 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2309 2343 3474 3508 4753 4787 1903
Size: 34 372 34 1131 34 1245 34 3678
Fragments arranged by size:
3678 1245 1131 372 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3564 4454 5664 3564
Size: 890 1210 4462
Fragments arranged by size:
4462 1210 890
BclI T'GATC_A
Cuts at: 1548 2527 1548
Size: 979 5583
BfrBI ATG'CAT
Cuts at: 3317 3471 3317
Size: 154 6408
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5173 136
Size: 107 122 71 631 605 3501 1525
Fragments arranged by size:
3501 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2584 6557 2584
Size: 3973 2589
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 2356 2372 4623 5779 1790
Size: 566 16 2251 1156 2573
Fragments arranged by size:
2573 2251 1156 566 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3764 4293
Size: 416 53 83 399 24 1487 860 529
Cuts at: 4293 5251 442
Size: 958 1753
Fragments arranged by size:
1753 1487 958 860 529 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 2219
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2214 2717 3342 3600 3711 5222 1239
Size: 812 163 503 625 258 111 1511 2579
Fragments arranged by size:
2579 1511 812 625 503 258 163 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 4687 5552 5850 2906
Size: 1781 865 298 3618
Fragments arranged by size:
3618 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5225 882
Size: 617 3726 2219
Fragments arranged by size:
3726 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4353 493
Size: 711 3149 2702
Fragments arranged by size:
3149 2702 711
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4369 4751 5386 275
Size: 53 83 186 936 2836 382 635 1451
Fragments arranged by size:
2836 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3573 3603 1178
Size: 30 165 30 2170 30 4137
Fragments arranged by size:
4137 2170 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3341 1186
Size: 2155 4407
BseYI C'CCAG_C
Cuts at: 2727 5765 6261 2727
Size: 3038 496 3028
Fragments arranged by size:
3038 3028 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 5425
Fragments arranged by size:
5425 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4012 4773 5801 1540
Size: 275 186 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2356 2372 3762 4623 4708 5779 2356
Size: 16 1390 861 85 1071 3139
Fragments arranged by size:
3139 1390 1071 861 85 16
BsmI GAATG_Cn'
Cuts at: 2352 3471 4073 4166 2352
Size: 1119 602 93 4748
Fragments arranged by size:
4748 1119 602 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2924 2924
Size: 6562
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2279 2356 2372 3151 3762 3881 4623
Size: 399 489 77 16 779 611 119 742
Cuts at: 4623 4708 5779 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 611 489 399 119
85 77 16
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 2985 4451 6181 2985
Size: 1466 1730 3366
Fragments arranged by size:
3366 1730 1466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3917 844
Size: 3073 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 3179 5057 5231 65
Size: 1183 1462 469 1878 174 1396
Fragments arranged by size:
1878 1462 1396 1183 469 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5206 5393 1700
Size: 110 3396 187 2869
Fragments arranged by size:
3396 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6562
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 2246 2213
Size: 33 6529
BstYI r'GATC_y
Cuts at: 1972 2584 2805 3739 3892 4259 4644 4661 6113
Size: 612 221 934 153 367 385 17 1452
Cuts at: 6113 6199 6211 6557 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 612 385 367 346 221
153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2476 2800 513
Size: 552 1411 324 4275
Fragments arranged by size:
4275 1411 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 4078 4872 4892 4078
Size: 794 20 5748
Fragments arranged by size:
5748 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DraIII CAC_nnn'GTG
Cuts at: 2697 2981 2697
Size: 284 6278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2687 5569 809
Size: 507 1371 2882 1802
Fragments arranged by size:
2882 1802 1371 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3526 4009 4898
Size: 54 1425 50 275 18 1696 483 889
Cuts at: 4898 6527 8
Size: 1629 43
Fragments arranged by size:
1696 1629 1425 889 483 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2562 4492 1353
Size: 1209 1930 3423
Fragments arranged by size:
3423 1930 1209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5149 5523 5669 387
Size: 1064 138 3560 374 146 1280
Fragments arranged by size:
3560 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2214 2414 2717 2967 3342 3600
Size: 393 419 163 200 303 250 375 258
Cuts at: 3600 3711 4625 5222 6009 6277 1239
Size: 111 914 597 787 268 1524
Fragments arranged by size:
1524 914 787 597 419 393 375 303
268 258 250 200 163 111
EcoO109I rG'GnC_Cy
Cuts at: 2680 2680
Size: 6562
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 5709 1678
Size: 4031 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2797 2829 3533 3565 5203
Size: 32 135 32 1225 32 704 32 1638
Cuts at: 5203 5235 5277 5309 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1638 1225 704 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3346 3646 3906 4152 669
Size: 988 281 1408 300 260 246 3079
Fragments arranged by size:
3079 1408 988 300 281 260 246
HindIII A'AGCT_T
Cuts at: 748 2649 748
Size: 1901 4661
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 2613 3887 2613
Size: 1274 5288
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2838 5676 5860 1305
Size: 1533 2838 184 2007
Fragments arranged by size:
2838 2007 1533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3528 10
Size: 54 1425 343 1696 3044
Fragments arranged by size:
3044 1696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3712 4520 4879 5038 518
Size: 648 2546 808 359 159 2042
Fragments arranged by size:
2546 2042 808 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2910 3610 3978 4655 5803 6048 1606
Size: 1304 700 368 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 700 677 368 245
NcoI C'CATG_G
Cuts at: 513 1065 2476 2800 513
Size: 552 1411 324 4275
Fragments arranged by size:
4275 1411 552 324
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NsiI A_TGCA'T
Cuts at: 3319 3473 3319
Size: 154 6408
NspI r_CATG'y
Cuts at: 1132 1229 3317 3920 5465 1132
Size: 97 2088 603 1545 2229
Fragments arranged by size:
2229 2088 1545 603 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6562
PciI A'CATG_T
Cuts at: 5461 5461
Size: 6562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3109 1108
Size: 2001 4561
PfoI T'CCnGG_A
Cuts at: 3392 3392
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 2680
Size: 6562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 3414 4132 3414
Size: 718 5844
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2910 2910
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 4810 6522 4810
Size: 1712 4850
SfcI C'TryA_G
Cuts at: 826 1045 2297 3131 3304 3910 5045 5726 5917
Size: 219 1252 834 173 606 1135 681 191
Cuts at: 5917 826
Size: 1471
Fragments arranged by size:
1471 1252 1135 834 681 606 219 191
173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2921 4666 5567 5829 820
Size: 197 926 978 1745 901 262 1553
Fragments arranged by size:
1745 1553 978 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3317 3920 1132
Size: 2185 603 3774
Fragments arranged by size:
3774 2185 603
SspI AAT'ATT
Cuts at: 5 52 2670 4486 5
Size: 47 2618 1816 2081
Fragments arranged by size:
2618 2081 1816 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6562
StyI C'CwwG_G
Cuts at: 513 1065 2476 2800 3504 4001 513
Size: 552 1411 324 704 497 3074
Fragments arranged by size:
3074 1411 704 552 497 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3116 4589 4606 4759 4944 5389 325
Size: 1255 1536 1473 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1473 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4808 6520 96
Size: 274 80 33 51 1443 2831 1712 138
Fragments arranged by size:
2831 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 6522 6554 2457
Size: 32 4033 32 2465
Fragments arranged by size:
4033 2465 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 3496 3804 2735
Size: 761 308 5493
Fragments arranged by size:
5493 761 308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 2427 3552 4691 2427
Size: 1125 1139 4298
Fragments arranged by size:
4298 1139 1125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI