Restriction map for
pFN21AB4144
(Circular) (Six-base) MAPSORT of: pFN21AB4144.seq Check: 9853 from: 1 to: 5173
pFN21AB4144 5173 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2133 2133
Size: 5173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2494 2494
Size: 5173
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3236 4620 4888 1632
Size: 548 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 2450 1166
Size: 1284 3889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5173
AlwNI CAG_nnn'CTG
Cuts at: 2125 2916 4488 2125
Size: 791 1572 2810
Fragments arranged by size:
2810 1572 791
ApaLI G'TGCA_C
Cuts at: 3230 4386 3230
Size: 1156 4017
ApoI r'AATT_y
Cuts at: 1493 2482 2708 2860 4969 1493
Size: 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2370 2498 1870
Size: 73 427 128 4545
Fragments arranged by size:
4545 427 128 73
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 2494 3949 618
Size: 325 217 627 596 111 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 217 111
BanII G_rGCy'C
Cuts at: 1391 2439 2492 1391
Size: 1048 53 4072
Fragments arranged by size:
4072 1048 53
BbeI G_GCGC'C
Cuts at: 2387 2387
Size: 5173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3364 3398 1903
Size: 34 1427 34 3678
Fragments arranged by size:
3678 1427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4275 3065
Size: 1210 3963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3784 136
Size: 107 122 71 631 605 2112 1525
Fragments arranged by size:
2112 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5168 5168
Size: 5173
BlpI GC'TnA_GC
Cuts at: 2075 2590 2075
Size: 515 4658
Bme1580I G_kGCm'C
Cuts at: 1790 2457 3234 4390 1790
Size: 667 777 1156 2573
Fragments arranged by size:
2573 1156 777 667
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3862 442
Size: 416 53 83 399 24 1487 958 1753
Fragments arranged by size:
1753 1487 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2330 3833 1239
Size: 1022 69 1503 2579
Fragments arranged by size:
2579 1503 1022 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3298 4163 4461 2165
Size: 1133 865 298 2877
Fragments arranged by size:
2877 1133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2138 3836 882
Size: 617 639 1698 2219
Fragments arranged by size:
2219 1698 639 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 493
Size: 711 1760 2702
Fragments arranged by size:
2702 1760 711
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 2384 2980 3362
Size: 53 83 186 936 786 65 596 382
Cuts at: 3362 3997 275
Size: 635 1451
Fragments arranged by size:
1451 936 786 635 596 382 186 83
65 53
BsaWI w'CCGG_w
Cuts at: 3606 4004 4278 4425 4836 3606
Size: 398 274 147 411 3943
Fragments arranged by size:
3943 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2249 2431 1186
Size: 973 90 182 3928
Fragments arranged by size:
3928 973 182 90
BseYI C'CCAG_C
Cuts at: 4376 4872 4376
Size: 496 4677
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4600
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2623 3384 4412 1540
Size: 275 186 622 761 1028 2301
Fragments arranged by size:
2301 1028 761 622 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2439 3234 3319 4390 2076
Size: 363 795 85 1071 2859
Fragments arranged by size:
2859 1071 795 363 85
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2439 2457 2492 3234 3319 4390
Size: 399 286 363 18 35 742 85 1071
Cuts at: 4390 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 363 286 85 35
18
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2528 844
Size: 1289 395 3489
Fragments arranged by size:
3489 1289 395
BsrBI CCG'CTC
Cuts at: 1394 1823 2245 3060 1394
Size: 429 422 815 3507
Fragments arranged by size:
3507 815 429 422
BsrDI GCAATG_nn'
Cuts at: 65 1248 3668 3842 65
Size: 1183 2420 174 1396
Fragments arranged by size:
2420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3817 4004 1700
Size: 110 2007 187 2869
Fragments arranged by size:
2869 2007 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5173
BssHII G'CGCG_C
Cuts at: 1925 2357 1925
Size: 432 4741
BssSI C'ACGA_G
Cuts at: 3233 4245 3233
Size: 1012 4161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5173
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 2149 1184
Size: 965 4208
BstYI r'GATC_y
Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168
Size: 531 367 385 17 1452 86 12 346
Cuts at: 5168 1972
Size: 1977
Fragments arranged by size:
1977 1452 531 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2188 2364 513
Size: 552 1123 176 3322
Fragments arranged by size:
3322 1123 552 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 3566
Fragments arranged by size:
3566 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3483 3503 2689
Size: 794 20 4359
Fragments arranged by size:
4359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2477 2477
Size: 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2397 2620
Size: 54 1425 50 275 18 369 198 223
Cuts at: 2620 3509 5138 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 369 275 223 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2620 1537
Size: 275 808 4090
Fragments arranged by size:
4090 808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 1353
Size: 1750 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 5173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2261 2330 3236 3833 4620 4888
Size: 393 548 81 69 906 597 787 268
Cuts at: 4888 1239
Size: 1524
Fragments arranged by size:
1524 906 787 597 548 393 268 81
69
EcoO109I rG'GnC_Cy
Cuts at: 2375 2375
Size: 5173
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 2345 2387 4320 1678
Size: 667 42 1933 2531
Fragments arranged by size:
2531 1933 667 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2078 2110 3814
Size: 32 135 32 427 32 47 32 1704
Cuts at: 3814 3846 3888 3920 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1704 427 135 47 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2763 669
Size: 988 281 579 246 3079
Fragments arranged by size:
3079 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KasI G'GCGC_C
Cuts at: 2383 2383
Size: 5173
KpnI G_GTAC'C
Cuts at: 2498 2498
Size: 5173
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4287 4471 1305
Size: 2982 184 2007
Fragments arranged by size:
2982 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 10
Size: 54 1425 343 567 2784
Fragments arranged by size:
2784 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2450 3131 3490 3649 518
Size: 648 936 348 681 359 159 2042
Fragments arranged by size:
2042 936 681 648 359 348 159
MspA1I CmG'CkG
Cuts at: 1606 2190 2205 2589 3266 4414 4659 1606
Size: 584 15 384 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 584 384 245 15
NarI GG'CG_CC
Cuts at: 2384 2384
Size: 5173
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4621
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5173
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2305 2531 4076 1132
Size: 97 1076 226 1545 2229
Fragments arranged by size:
2229 1545 1076 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5173
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2375 2375
Size: 5173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2177 2209 2177
Size: 32 5141
PstI C_TGCA'G
Cuts at: 830 2265 2349 2525 830
Size: 1435 84 176 3478
Fragments arranged by size:
3478 1435 176 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
PvuII CAG'CTG
Cuts at: 2205 2205
Size: 5173
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 5173
SacII CC_GC'GG
Cuts at: 2191 2191
Size: 5173
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SbfI CC_TGCA'GG
Cuts at: 2525 2525
Size: 5173
ScaI AGT'ACT
Cuts at: 3421 5133 3421
Size: 1712 3461
SfcI C'TryA_G
Cuts at: 826 1045 2261 2345 2521 3656 4337 4528 826
Size: 219 1216 84 176 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 219 191 176 84
SfoI GGC'GCC
Cuts at: 2385 2385
Size: 5173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2372 2500 1872
Size: 500 128 4545
Fragments arranged by size:
4545 500 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3277 4178 4440 820
Size: 197 926 237 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 2964 493
Size: 2471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2305 2531 1132
Size: 1173 226 3774
Fragments arranged by size:
3774 1173 226
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5173
StyI C'CwwG_G
Cuts at: 513 1065 2139 2391 2612 513
Size: 552 1074 252 221 3074
Fragments arranged by size:
3074 1074 552 252 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3200 3217 3370 3555 4000 325
Size: 1255 1620 17 153 185 445 1498
Fragments arranged by size:
1620 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3419 5131 96
Size: 274 80 33 51 1443 1442 1712 138
Fragments arranged by size:
1712 1443 1442 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5133 5165 5133
Size: 32 5141
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5173
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5173
XmaI C'CCGG_G
Cuts at: 1870 2370 2498 1870
Size: 500 128 4545
Fragments arranged by size:
4545 500 128
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI