Restriction map for
pFN21AB4141
(Circular) (Six-base) MAPSORT of: pFN21AB4141.seq Check: 8990 from: 1 to: 6952
pFN21AB4141 6952 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3911 3911
Size: 6952
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 4273 4273
Size: 6952
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 3547 3947 4006 5015 6399 6667 1632
Size: 855 1060 400 59 1009 1384 268 1917
Fragments arranged by size:
1917 1384 1060 1009 855 400 268 59
AfeI AGC'GCT
Cuts at: 2993 2993
Size: 6952
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6755
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 5681 5681
Size: 6952
AleI CACnn'nnGTG
Cuts at: 1166 2562 1166
Size: 1396 5556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3249 3725 1184
Size: 2065 476 4411
Fragments arranged by size:
4411 2065 476
AlwNI CAG_nnn'CTG
Cuts at: 2069 2777 3280 4695 6267 2069
Size: 708 503 1415 1572 2754
Fragments arranged by size:
2754 1572 1415 708 503
ApaI G_GGCC'C
Cuts at: 2444 3084 3475 2444
Size: 640 391 5921
Fragments arranged by size:
5921 640 391
ApaLI G'TGCA_C
Cuts at: 2379 5009 6165 2379
Size: 2630 1156 3166
Fragments arranged by size:
3166 2630 1156
ApoI r'AATT_y
Cuts at: 1493 3256 4261 4487 4639 6748 1493
Size: 1763 1005 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 1005 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2132 2597 2753 2867 3467 3829
Size: 73 147 42 465 156 114 600 362
Cuts at: 3829 4277 1870
Size: 448 4545
Fragments arranged by size:
4545 600 465 448 362 156 147 114
73 42
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2621 3872 4273 5728 618
Size: 325 217 627 834 1251 401 1455 1842
Fragments arranged by size:
1842 1455 1251 834 627 401 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2171 2444 2520 3084 3475 3773 4271
Size: 700 80 273 76 564 391 298 498
Cuts at: 4271 1391
Size: 4072
Fragments arranged by size:
4072 700 564 498 391 298 273 80
76
BbeI G_GCGC'C
Cuts at: 2625 2625
Size: 6952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3102 3618 4199 928
Size: 971 1203 516 581 3681
Fragments arranged by size:
3681 1203 971 581 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5143 5177 1903
Size: 34 3206 34 3678
Fragments arranged by size:
3678 3206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4844 6054 4844
Size: 1210 5742
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6952
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 2941 5563
Size: 107 122 71 631 605 1072 197 2622
Cuts at: 5563 136
Size: 1525
Fragments arranged by size:
2622 1525 1072 631 605 197 122 107
71
BglII A'GATC_T
Cuts at: 6947 6947
Size: 6952
BlpI GC'TnA_GC
Cuts at: 3839 4071 4369 3839
Size: 232 298 6422
Fragments arranged by size:
6422 298 232
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2383 2444 3084 3475 5013 6169 1790
Size: 343 250 61 640 391 1538 1156 2573
Fragments arranged by size:
2573 1538 1156 640 391 343 250 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3538 4683 5641
Size: 416 53 83 399 24 2121 1145 958
Cuts at: 5641 442
Size: 1753
Fragments arranged by size:
2121 1753 1145 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2203 2681 2990 3737 5612 1239
Size: 923 41 478 309 747 1875 2579
Fragments arranged by size:
2579 1875 923 747 478 309 41
Bpu10I CC'TnA_GC
Cuts at: 2766 2766
Size: 6952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2549 3637 3989 5077 5942 6240 2042
Size: 507 1088 352 1088 865 298 2754
Fragments arranged by size:
2754 1088 1088 865 507 352 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 2880 3452 4229 5615 882
Size: 617 554 827 572 777 1386 2219
Fragments arranged by size:
2219 1386 827 777 617 572 554
BsaAI yAC'GTr
Cuts at: 493 1204 2233 4743 493
Size: 711 1029 2510 2702
Fragments arranged by size:
2702 2510 1029 711
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 3421 4083 4759
Size: 53 83 186 936 1089 799 662 676
Cuts at: 4759 5141 5776 275
Size: 382 635 1451
Fragments arranged by size:
1451 1089 936 799 676 662 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2013 2506 5385 5783 6057 6204 6615 2013
Size: 493 2879 398 274 147 411 2350
Fragments arranged by size:
2879 2350 493 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6727
Fragments arranged by size:
6727 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2267 2294 2297 2300 2375 2435 2615
Size: 1078 3 27 3 3 75 60 180
Cuts at: 2615 2687 4211 1186
Size: 72 1524 3927
Fragments arranged by size:
3927 1524 1078 180 75 72 60 27
3 3 3
BseYI C'CCAG_C
Cuts at: 2825 3144 3531 3765 4015 6155 6651 2825
Size: 319 387 234 250 2140 496 3126
Fragments arranged by size:
3126 2140 496 387 319 250 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4049 1163
Size: 573 2313 4066
Fragments arranged by size:
4066 2313 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4176 4402 5163 6191 1540
Size: 275 186 2175 226 761 1028 2301
Fragments arranged by size:
2301 2175 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2383 5013 5098 6169 2383
Size: 2630 85 1071 3166
Fragments arranged by size:
3166 2630 1071 85
BsmI GAATG_Cn'
Cuts at: 4463 4556 4463
Size: 93 6859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2133 2171 2383 2444 2520 3084
Size: 399 301 42 38 212 61 76 564
Cuts at: 3084 3475 3773 4271 5013 5098 6169 1391
Size: 391 298 498 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 498 399 391 301
298 212 85 76 61 42 38
BspEI T'CCGG_A
Cuts at: 2506 6615 2506
Size: 4109 2843
BspHI T'CATG_A
Cuts at: 4053 4841 6571 4053
Size: 788 1730 4434
Fragments arranged by size:
4434 1730 788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2944 3911 4307 844
Size: 2100 967 396 3489
Fragments arranged by size:
3489 2100 967 396
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 3828 4839 1394
Size: 429 214 1791 1011 3507
Fragments arranged by size:
3507 1791 1011 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 2968 4065 5447 5621 65
Size: 1183 1720 1097 1382 174 1396
Fragments arranged by size:
1720 1396 1382 1183 1097 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 5596 5783 1700
Size: 110 808 2978 187 2869
Fragments arranged by size:
2978 2869 808 187 110
BsrGI T'GTAC_A
Cuts at: 96 3660 4245 96
Size: 3564 585 2803
Fragments arranged by size:
3564 2803 585
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 2459 2834 2948 4142 5012 6024 2459
Size: 375 114 1194 870 1012 3387
Fragments arranged by size:
3387 1194 1012 870 375 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2349 794
Size: 1555 5397
BstBI TT'CG_AA
Cuts at: 5756 5756
Size: 6952
BstEII G'GTnAC_C
Cuts at: 1184 3973 1184
Size: 2789 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 3133 3883 3133
Size: 750 6202
BstYI r'GATC_y
Cuts at: 1972 3735 4182 4282 4649 5034 5051 6503 6589
Size: 1763 447 100 367 385 17 1452 86
Cuts at: 6589 6601 6947 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1763 1452 447 385 367 346 100
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2277 2319 2639 2778 2277
Size: 42 320 139 6451
Fragments arranged by size:
6451 320 139 42
BtgI C'CryG_G
Cuts at: 513 1065 2004 2452 3783 3978 513
Size: 552 939 448 1331 195 3487
Fragments arranged by size:
3487 1331 939 552 448 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3367 3523 487
Size: 683 56 2141 156 3916
Fragments arranged by size:
3916 2141 683 156 56
BtsI GCAGTG_nn'
Cuts at: 4468 5262 5282 4468
Size: 794 20 6138
Fragments arranged by size:
6138 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3027 3048 3062 3083 529
Size: 35 2463 21 14 21 4398
Fragments arranged by size:
4398 2463 35 21 21 14
DraI TTT'AAA
Cuts at: 3327 4256 3327
Size: 929 6023
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5959 809
Size: 507 4643 1802
Fragments arranged by size:
4643 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2159 2455 2491
Size: 54 1425 50 275 18 329 296 36
Cuts at: 2491 3414 3553 3795 3995 4131 4399 5288 6917
Size: 923 139 242 200 136 268 889 1629
Cuts at: 6917 8
Size: 43
Fragments arranged by size:
1629 1425 923 889 329 296 275 268
242 200 139 136 54 50 43 36
18
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2922 3302 4882 1353
Size: 1569 380 1580 3423
Fragments arranged by size:
3423 1580 1569 380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5539 5913 6059 387
Size: 1064 138 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2203 2487 2681 2990 3547 3737
Size: 393 530 41 284 194 309 557 190
Cuts at: 3737 3947 4006 5015 5612 6399 6667 1239
Size: 210 59 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 597 557 530 393 309
284 268 210 194 190 59 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2769 2867 2276
Size: 493 98 6361
Fragments arranged by size:
6361 493 98
EcoO109I rG'GnC_Cy
Cuts at: 2066 2137 2269 2656 2774 2919 3080 3081 3471
Size: 71 132 387 118 145 161 1 390
Cuts at: 3471 3922 2066
Size: 451 5096
Fragments arranged by size:
5096 451 390 387 161 145 132 118
71 1
EcoRI G'AATT_C
Cuts at: 3256 4261 4639 3256
Size: 1005 378 5569
Fragments arranged by size:
5569 1005 378
FspI TGC'GCA
Cuts at: 5458 5458
Size: 6952
HaeII r_GCGC'y
Cuts at: 1678 2625 2739 2995 6099 1678
Size: 947 114 256 3104 2531
Fragments arranged by size:
3104 2531 947 256 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 2201 2207 2233
Size: 32 135 32 478 32 119 6 26
Cuts at: 2233 2239 2504 2536 4068 4100 5593 5625 5667
Size: 6 265 32 1532 32 1493 32 42
Cuts at: 5667 5699 1373
Size: 32 2626
Fragments arranged by size:
2626 1532 1493 478 265 135 119 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3016 4296 4542 669
Size: 988 281 1078 1280 246 3079
Fragments arranged by size:
3079 1280 1078 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2018 748
Size: 1270 5682
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KasI G'GCGC_C
Cuts at: 2621 2621
Size: 6952
KpnI G_GTAC'C
Cuts at: 4277 4277
Size: 6952
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 3501 4156 4191 6066 6250 1305
Size: 720 1476 655 35 1875 184 2007
Fragments arranged by size:
2007 1875 1476 720 655 184 35
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3416 3797 4133 10
Size: 54 1425 343 1584 381 336 2829
Fragments arranged by size:
2829 1584 1425 381 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2562 3182 3404 4910 5269 5428 518
Size: 648 1396 620 222 1506 359 159 2042
Fragments arranged by size:
2042 1506 1396 648 620 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 2817 3148 3715 3963 4015 4243 4368 5045
Size: 1211 331 567 248 52 228 125 677
Cuts at: 5045 6193 6438 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1211 1148 677 567 331 248 245
228 125 52
NarI GG'CG_CC
Cuts at: 2622 2622
Size: 6952
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5461
Fragments arranged by size:
5461 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6952
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2044 3430 4310 5855 1132
Size: 97 815 1386 880 1545 2229
Fragments arranged by size:
2229 1545 1386 880 815 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6952
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 1108
Size: 1584 5368
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2260 5021 5053 6534 6566 2228
Size: 32 2761 32 1481 32 2614
Fragments arranged by size:
2761 2614 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2656 2919 2066
Size: 590 263 6099
Fragments arranged by size:
6099 590 263
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspOMI G'GGCC_C
Cuts at: 2440 3080 3471 2440
Size: 640 391 5921
Fragments arranged by size:
5921 640 391
PstI C_TGCA'G
Cuts at: 830 2822 3714 4049 4304 830
Size: 1992 892 335 255 3478
Fragments arranged by size:
3478 1992 892 335 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2817 3148 3715 3963 4015 2817
Size: 331 567 248 52 5754
Fragments arranged by size:
5754 567 331 248 52
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 5200 6912 5200
Size: 1712 5240
SexAI A'CCwGG_T
Cuts at: 2062 2062
Size: 6952
SfcI C'TryA_G
Cuts at: 826 1045 2787 2818 3710 4045 4300 5435 6116
Size: 219 1742 31 892 335 255 1135 681
Cuts at: 6116 6307 826
Size: 191 1471
Fragments arranged by size:
1742 1471 1135 892 681 335 255 219
191 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 2941 2941
Size: 6952
SfoI GGC'GCC
Cuts at: 2623 2623
Size: 6952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 2618 1700
Size: 918 6034
SmaI CCC'GGG
Cuts at: 1872 3469 4279 1872
Size: 1597 810 4545
Fragments arranged by size:
4545 1597 810
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2528 3616 4004 5056 5957
Size: 197 926 78 507 1088 388 1052 901
Cuts at: 5957 6219 820
Size: 262 1553
Fragments arranged by size:
1553 1088 1052 926 901 507 388 262
197 78
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 3430 4310 1132
Size: 2298 880 3774
Fragments arranged by size:
3774 2298 880
SspI AAT'ATT
Cuts at: 5 52 4876 5
Size: 47 4824 2081
Fragments arranged by size:
4824 2081 47
StuI AGG'CCT
Cuts at: 1517 2795 3560 1517
Size: 1278 765 4909
Fragments arranged by size:
4909 1278 765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2978 3907 4391 513
Size: 552 939 111 863 929 484 3074
Fragments arranged by size:
3074 939 929 863 552 484 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 3158 3323 3665 4162 4979 4996
Size: 1255 1238 340 165 342 497 817 17
Cuts at: 4996 5149 5334 5779 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1238 817 497 445 342 340
185 165 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3660 4245 5198
Size: 274 80 33 51 1443 1683 585 953
Cuts at: 5198 6910 96
Size: 1712 138
Fragments arranged by size:
1712 1683 1443 953 585 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 6912 6944 2174
Size: 32 4706 32 2182
Fragments arranged by size:
4706 2182 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2064 1316
Size: 748 6204
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 2549 3790 2207
Size: 342 1241 5369
Fragments arranged by size:
5369 1241 342
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 3467 4277 1870
Size: 1597 810 4545
Fragments arranged by size:
4545 1597 810
XmnI GAAnn'nnTTC
Cuts at: 5081 5081
Size: 6952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SrfI SwaI