Restriction map for
pFN21AB4140
(Circular) (Six-base) MAPSORT of: pFN21AB4140.seq Check: 6550 from: 1 to: 6946
pFN21AB4140 6946 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3461 4090 3461
Size: 629 6317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2265 4756 278
Size: 53 83 186 936 729 2491 2468
Fragments arranged by size:
2491 2468 936 729 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3273 3817 4041 4289 1937
Size: 1336 544 224 248 4594
Fragments arranged by size:
4594 1336 544 248 224
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3872 4238 5009 6393 6661 1632
Size: 2240 366 771 1384 268 1917
Fragments arranged by size:
2240 1917 1384 771 366 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 2481 3787 5845 6822 1203
Size: 1278 1306 2058 977 1327
Fragments arranged by size:
2058 1327 1306 1278 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3399 1184
Size: 2215 4731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 6914
AlwNI CAG_nnn'CTG
Cuts at: 2184 2420 2440 2983 3950 4096 4105 4140 4689
Size: 236 20 543 967 146 9 35 549
Cuts at: 4689 6261 2184
Size: 1572 2869
Fragments arranged by size:
2869 1572 967 549 543 236 146 35
20 9
ApaI G_GGCC'C
Cuts at: 3998 4216 3998
Size: 218 6728
ApaLI G'TGCA_C
Cuts at: 3332 5003 6159 3332
Size: 1671 1156 4119
Fragments arranged by size:
4119 1671 1156
ApoI r'AATT_y
Cuts at: 1493 3099 3727 4255 4481 4633 6742 1493
Size: 1606 628 528 226 152 2109 1697
Fragments arranged by size:
2109 1697 1606 628 528 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 4068 4271 1870
Size: 73 802 1323 203 4545
Fragments arranged by size:
4545 1323 802 203 73
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3146 3473 4267 5722 618
Size: 325 217 627 1359 327 794 1455 1842
Fragments arranged by size:
1842 1455 1359 794 627 327 325 217
BanII G_rGCy'C
Cuts at: 1391 2174 2811 3709 3998 4152 4216 4265 1391
Size: 783 637 898 289 154 64 49 4072
Fragments arranged by size:
4072 898 783 637 289 154 64 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2265 928
Size: 971 366 5609
Fragments arranged by size:
5609 971 366
BbvCI CC'TCA_GC
Cuts at: 2310 3798 2310
Size: 1488 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2212 2246 3242 3276 5137 5171 1903
Size: 34 275 34 996 34 1861 34 3678
Fragments arranged by size:
3678 1861 996 275 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3916 3993 4230
Size: 107 122 71 631 605 2244 77 237
Cuts at: 4230 5557 136
Size: 1327 1525
Fragments arranged by size:
2244 1525 1327 631 605 237 122 107
77 71
BglII A'GATC_T
Cuts at: 2898 6941 2898
Size: 4043 2903
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 3336 3478 3564 3998 4216 5007 6163 1790
Size: 1546 142 86 434 218 791 1156 2573
Fragments arranged by size:
2573 1546 1156 791 434 218 142 86
BmgBI CAC'GTC
Cuts at: 1318 2241 2431 3574 1318
Size: 923 190 1143 4690
Fragments arranged by size:
4690 1143 923 190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3487 4677 5635
Size: 416 53 83 399 24 2070 1190 958
Cuts at: 5635 442
Size: 1753
Fragments arranged by size:
2070 1753 1190 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3366 1056
Size: 2310 4636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2293 2486 2528 2543 3731 4082 5606 1239
Size: 1054 193 42 15 1188 351 1524 2579
Fragments arranged by size:
2579 1524 1188 1054 351 193 42 15
Bpu10I CC'TnA_GC
Cuts at: 2310 2398 3798 3870 2310
Size: 88 1400 72 5386
Fragments arranged by size:
5386 1400 88 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3581 3949 5071 5936 6234 3062
Size: 519 368 1122 865 298 3774
Fragments arranged by size:
3774 1122 865 519 368 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 5609 882
Size: 617 810 3300 2219
Fragments arranged by size:
3300 2219 810 617
BsaAI yAC'GTr
Cuts at: 493 1204 2484 3331 4737 493
Size: 711 1280 847 1406 2702
Fragments arranged by size:
2702 1406 1280 847 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 2460 4753 5135
Size: 53 83 186 936 729 198 2293 382
Cuts at: 5135 5770 275
Size: 635 1451
Fragments arranged by size:
2293 1451 936 729 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 2727 5379 5777 6051 6198 6609 2727
Size: 2652 398 274 147 411 3064
Fragments arranged by size:
3064 2652 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 6085
Fragments arranged by size:
6085 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2327 2540 1186
Size: 837 304 213 5592
Fragments arranged by size:
5592 837 304 213
BseYI C'CCAG_C
Cuts at: 2167 2491 2628 3313 3479 3644 4105 6149 6645
Size: 324 137 685 166 165 461 2044 496
Cuts at: 6645 2167
Size: 2468
Fragments arranged by size:
2468 2044 685 496 461 324 166 165
137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 2453 2907 2912 3041 3272 3527
Size: 573 610 107 454 5 129 231 255
Cuts at: 3527 3689 3815 3834 1163
Size: 162 126 19 4275
Fragments arranged by size:
4275 610 573 454 255 231 162 129
126 107 19 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 4396 5157 6185 1540
Size: 275 186 101 2294 761 1028 2301
Fragments arranged by size:
2301 2294 1028 761 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2760 3336 3876 5007 5092 6163 2760
Size: 576 540 1131 85 1071 3543
Fragments arranged by size:
3543 1131 1071 576 540 85
BsmI GAATG_Cn'
Cuts at: 4457 4550 4457
Size: 93 6853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2760 2811 3336 3478 3564 3709
Size: 399 384 586 51 525 142 86 145
Cuts at: 3709 3876 3998 4152 4216 4265 5007 5092 6163
Size: 167 122 154 64 49 742 85 1071
Cuts at: 6163 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 586 525 399 384 167
154 145 142 122 86 85 64 51
49
BspEI T'CCGG_A
Cuts at: 2727 6609 2727
Size: 3882 3064
BspHI T'CATG_A
Cuts at: 2476 4835 6565 2476
Size: 2359 1730 2857
Fragments arranged by size:
2857 2359 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 2799 2946 3461 4090 4301 844
Size: 1883 72 147 515 629 211 3489
Fragments arranged by size:
3489 1883 629 515 211 147 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 5441 5615 65
Size: 1183 2705 1488 174 1396
Fragments arranged by size:
2705 1488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2278 3033 3793 5590 5777 1700
Size: 110 468 755 760 1797 187 2869
Fragments arranged by size:
2869 1797 760 755 468 187 110
BsrGI T'GTAC_A
Cuts at: 96 4185 96
Size: 4089 2857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 5300
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstXI CCAn_nnnn'nTGG
Cuts at: 4036 4036
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 2898 4276 4643 5028 5045 6497 6583 6595
Size: 926 1378 367 385 17 1452 86 12
Cuts at: 6595 6941 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1378 926 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3807 4050 3807
Size: 243 6703
BtgI C'CryG_G
Cuts at: 513 1065 2096 2190 513
Size: 552 1031 94 5269
Fragments arranged by size:
5269 1031 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2209 3713 487
Size: 683 56 983 1504 3720
Fragments arranged by size:
3720 1504 983 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 2847 2924 3015 3515 3926 4462 5256 5276
Size: 629 77 91 500 411 536 794 20
Cuts at: 5276 2218
Size: 3888
Fragments arranged by size:
3888 794 629 536 500 411 91 77
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6911
DraI TTT'AAA
Cuts at: 3118 4250 3118
Size: 1132 5814
DraIII CAC_nnn'GTG
Cuts at: 3064 3403 3521 3064
Size: 339 118 6489
Fragments arranged by size:
6489 339 118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2253 3015
Size: 54 1425 50 275 18 385 38 762
Cuts at: 3015 3031 3512 4179 4231 4393 5282 6911 8
Size: 16 481 667 52 162 889 1629 43
Fragments arranged by size:
1629 1425 889 762 667 481 385 275
162 54 52 50 43 38 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 3083 4876 1353
Size: 1200 530 1793 3423
Fragments arranged by size:
3423 1793 1200 530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 5533 5907 6053 387
Size: 1064 138 570 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 570 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2293 2486 2528 2543 3731 3872 4082
Size: 393 661 193 42 15 1188 141 210
Cuts at: 4082 4238 5009 5606 6393 6661 1239
Size: 156 771 597 787 268 1524
Fragments arranged by size:
1524 1188 787 771 661 597 393 268
210 193 156 141 42 15
EcoO109I rG'GnC_Cy
Cuts at: 2772 3307 3487 3803 3994 3995 4212 4213 2772
Size: 535 180 316 191 1 217 1 5505
Fragments arranged by size:
5505 535 316 217 191 180 1 1
EcoRI G'AATT_C
Cuts at: 3727 4255 4633 3727
Size: 528 378 6040
Fragments arranged by size:
6040 528 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3622 3622
Size: 6946
FseI GG_CCGG'CC
Cuts at: 2282 3037 2282
Size: 755 6191
FspI TGC'GCA
Cuts at: 2651 5452 2651
Size: 2801 4145
HaeII r_GCGC'y
Cuts at: 1678 2085 2108 2184 2252 2983 6093 1678
Size: 407 23 76 68 731 3110 2531
Fragments arranged by size:
3110 2531 731 407 76 68 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 2903 2935 5587
Size: 32 135 32 1121 32 178 32 2652
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
2652 2626 1121 178 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3818 4042 4290 4536 669
Size: 988 281 1880 224 248 246 3079
Fragments arranged by size:
3079 1880 988 281 248 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6946
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 6060 6244 1305
Size: 840 3915 184 2007
Fragments arranged by size:
3915 2007 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 3514 4181 4233 10
Size: 54 1425 343 1185 497 667 52 2723
Fragments arranged by size:
2723 1425 1185 667 497 343 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 4904 5263 5422 518
Size: 648 3738 359 159 2042
Fragments arranged by size:
3738 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2098 2128 3422 3644 3653 4036 4060
Size: 416 76 30 1294 222 9 383 24
Cuts at: 4060 4362 5039 6187 6432 1606
Size: 302 677 1148 245 2120
Fragments arranged by size:
2120 1294 1148 677 416 383 302 245
222 76 30 24 9
NaeI GCC'GGC
Cuts at: 2280 3035 3795 2280
Size: 755 760 5431
Fragments arranged by size:
5431 760 755
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6394
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NgoMIV G'CCGG_C
Cuts at: 2278 3033 3793 2278
Size: 755 760 5431
Fragments arranged by size:
5431 760 755
NheI G'CTAG_C
Cuts at: 1052 3362 1052
Size: 2310 4636
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 3791 4304 5849 1132
Size: 97 2562 513 1545 2229
Fragments arranged by size:
2562 2229 1545 513 97
PasI CC'CwG_GG
Cuts at: 1348 3743 1348
Size: 2395 4551
PciI A'CATG_T
Cuts at: 3787 5845 3787
Size: 2058 4888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2420 2874 1108
Size: 1312 454 5180
Fragments arranged by size:
5180 1312 454
PfoI T'CCnGG_A
Cuts at: 2967 3627 2967
Size: 660 6286
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PmlI CAC'GTG
Cuts at: 2484 2484
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2772 3307 3487 3803 2772
Size: 535 180 316 5915
Fragments arranged by size:
5915 535 316 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 3994 4212 3994
Size: 218 6728
PstI C_TGCA'G
Cuts at: 830 2142 2336 2796 3643 4101 4243 4298 830
Size: 1312 194 460 847 458 142 55 3478
Fragments arranged by size:
3478 1312 847 460 458 194 142 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 3644 3653 4036 4060 3644
Size: 9 383 24 6530
Fragments arranged by size:
6530 383 24 9
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 3816 4040 4288 1936
Size: 1880 224 248 4594
Fragments arranged by size:
4594 1880 248 224
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 5194 6906 5194
Size: 1712 5234
SfcI C'TryA_G
Cuts at: 826 1045 2138 2332 2792 3106 3639 4097 4239
Size: 219 1093 194 460 314 533 458 142
Cuts at: 4239 4294 5429 6110 6301 826
Size: 55 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 533 460 458 314
219 194 191 142 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 4230 4230
Size: 6946
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3077 3596 3928 5050 5951 6213
Size: 197 926 1134 519 332 1122 901 262
Cuts at: 6213 820
Size: 1553
Fragments arranged by size:
1553 1134 1122 926 901 519 332 262
197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 1517
Size: 864 6082
StyI C'CwwG_G
Cuts at: 513 1065 3294 3300 3501 4385 513
Size: 552 2229 6 201 884 3074
Fragments arranged by size:
3074 2229 884 552 201 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4973 4990 5143 5328 5773 325
Size: 1255 3393 17 153 185 445 1498
Fragments arranged by size:
3393 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4185 5192 6904
Size: 274 80 33 51 1443 2208 1007 1712
Cuts at: 6904 96
Size: 138
Fragments arranged by size:
2208 1712 1443 1007 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3130 3162 6906 6938 3130
Size: 32 3744 32 3138
Fragments arranged by size:
3744 3138 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 3400 2902
Size: 498 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 5075 5075
Size: 6946
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2263 4754 276
Size: 53 83 186 936 729 2491 2468
Fragments arranged by size:
2491 2468 936 729 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfoI SrfI SwaI