Restriction map for
pFN21AB4124
(Circular) (Six-base) MAPSORT of: pFN21AB4124.seq Check: 9273 from: 1 to: 7684
pFN21AB4124 7684 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5494 278
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 5027 1937
Size: 1557 1533 4594
Fragments arranged by size:
4594 1557 1533
Acc65I G'GTAC_C
Cuts at: 2022 5005 2022
Size: 2983 4701
AclI AA'CG_TT
Cuts at: 1563 5811 6184 1563
Size: 4248 373 3063
Fragments arranged by size:
4248 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 3483 5747 7131 7399 1632
Size: 1229 622 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 1229 622 268
AflII C'TTAA_G
Cuts at: 820 1017 3274 820
Size: 197 2257 5230
Fragments arranged by size:
5230 2257 197
AflIII A'CryG_T
Cuts at: 1203 2702 4506 6583 7560 1203
Size: 1499 1804 2077 977 1327
Fragments arranged by size:
2077 1804 1499 1327 977
AgeI A'CCGG_T
Cuts at: 6515 6515
Size: 7684
AhdI GACnn_n'nnGTC
Cuts at: 6413 6413
Size: 7684
AleI CACnn'nnGTG
Cuts at: 1166 2513 2754 1166
Size: 1347 241 6096
Fragments arranged by size:
6096 1347 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2772 1184
Size: 1588 6096
AlwNI CAG_nnn'CTG
Cuts at: 2663 4355 5427 6999 2663
Size: 1692 1072 1572 3348
Fragments arranged by size:
3348 1692 1572 1072
ApaLI G'TGCA_C
Cuts at: 5741 6897 5741
Size: 1156 6528
ApoI r'AATT_y
Cuts at: 1493 2312 2672 3791 3917 4380 4424 4634 4653
Size: 819 360 1119 126 463 44 210 19
Cuts at: 4653 4993 5219 5371 7480 1493
Size: 340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 819 463 360 340 226
210 152 126 44 19
AseI AT'TA_AT
Cuts at: 160 1032 3510 6238 160
Size: 872 2478 2728 1606
Fragments arranged by size:
2728 2478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
AvaI C'yCGr_G
Cuts at: 1870 1943 4329 5009 1870
Size: 73 2386 680 4545
Fragments arranged by size:
4545 2386 680 73
BamHI G'GATC_C
Cuts at: 5014 5381 5014
Size: 367 7317
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 3800 5005 6460 618
Size: 325 217 627 235 1778 1205 1455 1842
Fragments arranged by size:
1842 1778 1455 1205 627 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2241 2962 4144 5003 1391
Size: 850 721 1182 859 4072
Fragments arranged by size:
4072 1182 859 850 721
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 2853 928
Size: 971 431 523 5759
Fragments arranged by size:
5759 971 523 431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3315 3349 5875 5909 1903
Size: 34 1378 34 2526 34 3678
Fragments arranged by size:
3678 2526 1378 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3158 5576 6786 3158
Size: 2418 1210 4056
Fragments arranged by size:
4056 2418 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7684
BfrBI ATG'CAT
Cuts at: 4151 4151
Size: 7684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4578 6295 136
Size: 107 122 71 631 605 2906 1717 1525
Fragments arranged by size:
2906 1717 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2872 7679 2872
Size: 4807 2877
BlpI GC'TnA_GC
Cuts at: 5101 5101
Size: 7684
Bme1580I G_kGCm'C
Cuts at: 1790 2781 5745 6901 1790
Size: 991 2964 1156 2573
Fragments arranged by size:
2964 2573 1156 991
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7684
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 5415 6373
Size: 416 53 83 399 24 943 3055 958
Cuts at: 6373 442
Size: 1753
Fragments arranged by size:
3055 1753 958 943 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3224 1056
Size: 2168 5516
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 7684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 2911 3619 6344 1239
Size: 1125 547 708 2725 2579
Fragments arranged by size:
2725 2579 1125 708 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2853 5809 6674 6972 2853
Size: 2956 865 298 3565
Fragments arranged by size:
3565 2956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6347 882
Size: 617 4848 2219
Fragments arranged by size:
4848 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5475 493
Size: 711 4271 2702
Fragments arranged by size:
4271 2702 711
BsaBI GATnn'nnATC
Cuts at: 7678 7678
Size: 7684
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5491 5873 6508 275
Size: 53 83 186 936 3958 382 635 1451
Fragments arranged by size:
3958 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 6117 6515 6789 6936 7347 4419
Size: 1698 398 274 147 411 4756
Fragments arranged by size:
4756 1698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2980 3010 1178
Size: 30 165 30 1577 30 5852
Fragments arranged by size:
5852 1577 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2914 1186
Size: 1728 5956
BseYI C'CCAG_C
Cuts at: 3174 3680 4573 6887 7383 3174
Size: 506 893 2314 496 3475
Fragments arranged by size:
3475 2314 893 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2736 2778 2988 3139 3930 1163
Size: 573 1000 42 210 151 791 4917
Fragments arranged by size:
4917 1000 791 573 210 151 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5134 5895 6923 1540
Size: 275 186 3133 761 1028 2301
Fragments arranged by size:
3133 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2885 4948 5745 5830 6901 2885
Size: 2063 797 85 1071 3668
Fragments arranged by size:
3668 2063 1071 797 85
BsmI GAATG_Cn'
Cuts at: 4206 4929 4974 5195 5288 4206
Size: 723 45 221 93 6602
Fragments arranged by size:
6602 723 221 93 45
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2781 2885 2962 4144 4948 5003
Size: 399 451 540 104 77 1182 804 55
Cuts at: 5003 5745 5830 6901 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1182 1071 804 742 540 451 399
104 85 77 55
BspEI T'CCGG_A
Cuts at: 7347 7347
Size: 7684
BspHI T'CATG_A
Cuts at: 4110 4920 5573 7303 4110
Size: 810 653 1730 4491
Fragments arranged by size:
4491 1730 810 653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3225 5039 844
Size: 2381 1814 3489
Fragments arranged by size:
3489 2381 1814
BsrBI CCG'CTC
Cuts at: 1394 1823 5571 1394
Size: 429 3748 3507
Fragments arranged by size:
3748 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 2448 3660 6179 6353 65
Size: 1183 1042 158 1212 2519 174 1396
Fragments arranged by size:
2519 1396 1212 1183 1042 174 158
BsrFI r'CCGG_y
Cuts at: 1700 1810 6328 6515 1700
Size: 110 4518 187 2869
Fragments arranged by size:
4518 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7684
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7684
BssSI C'ACGA_G
Cuts at: 5744 6756 5744
Size: 1012 6672
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7684
BstBI TT'CG_AA
Cuts at: 4672 6488 4672
Size: 1816 5868
BstEII G'GTnAC_C
Cuts at: 1184 2477 1184
Size: 1293 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 3182 3694 4392 3182
Size: 512 698 6474
Fragments arranged by size:
6474 698 512
BstYI r'GATC_y
Cuts at: 1972 2872 5014 5381 5766 5783 7235 7321 7333
Size: 900 2142 367 385 17 1452 86 12
Cuts at: 7333 7679 1972
Size: 346 1977
Fragments arranged by size:
2142 1977 1452 900 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2321 2321
Size: 7684
BtgI C'CryG_G
Cuts at: 513 1065 2004 2845 4563 513
Size: 552 939 841 1718 3634
Fragments arranged by size:
3634 1718 939 841 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3780 487
Size: 683 56 2554 4391
Fragments arranged by size:
4391 2554 683 56
BtsI GCAGTG_nn'
Cuts at: 2719 2749 2761 3940 5200 5994 6014 2719
Size: 30 12 1179 1260 794 20 4389
Fragments arranged by size:
4389 1260 1179 794 30 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7649
DraI TTT'AAA
Cuts at: 3790 4988 3790
Size: 1198 6486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6691 809
Size: 507 5375 1802
Fragments arranged by size:
5375 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3937 5131
Size: 54 1425 50 275 18 1012 1095 1194
Cuts at: 5131 6020 7649 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1194 1095 1012 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5131 1537
Size: 275 3319 4090
Fragments arranged by size:
4090 3319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3765 4734 5614 1353
Size: 2412 969 880 3423
Fragments arranged by size:
3423 2412 969 880
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6271 6645 6791 387
Size: 1064 138 4682 374 146 1280
Fragments arranged by size:
4682 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2364 2861 2911 3483 3619 5747 6344
Size: 393 732 497 50 572 136 2128 597
Cuts at: 6344 7131 7399 1239
Size: 787 268 1524
Fragments arranged by size:
2128 1524 787 732 597 572 497 393
268 136 50
EcoO109I rG'GnC_Cy
Cuts at: 3835 4261 3835
Size: 426 7258
EcoRI G'AATT_C
Cuts at: 3917 4653 4993 5371 3917
Size: 736 340 378 6230
Fragments arranged by size:
6230 736 378 340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2498 2498
Size: 7684
FspI TGC'GCA
Cuts at: 6190 6190
Size: 7684
HaeII r_GCGC'y
Cuts at: 1678 6831 1678
Size: 5153 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 6325 6357 6399
Size: 32 135 32 565 32 4156 32 42
Cuts at: 6399 6431 1373
Size: 32 2626
Fragments arranged by size:
4156 2626 565 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4288 5028 5274 669
Size: 988 281 2350 740 246 3079
Fragments arranged by size:
3079 2350 988 740 281 246
HindIII A'AGCT_T
Cuts at: 748 2501 748
Size: 1753 5931
HpaI GTT'AAC
Cuts at: 5274 5274
Size: 7684
KpnI G_GTAC'C
Cuts at: 2026 5009 2026
Size: 2983 4701
MfeI C'AATT_G
Cuts at: 5283 5283
Size: 7684
MluI A'CGCG_T
Cuts at: 7560 7560
Size: 7684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2918 4578 4630 4753 6798 6982 1305
Size: 813 800 1660 52 123 2045 184 2007
Fragments arranged by size:
2045 2007 1660 813 800 184 123 52
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5862
Fragments arranged by size:
5862 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2754 3209 3411 3665 3695 4065
Size: 648 1347 241 455 202 254 30 370
Cuts at: 4065 4962 4979 5642 6001 6160 518
Size: 897 17 663 359 159 2042
Fragments arranged by size:
2042 1347 897 663 648 455 370 359
254 241 202 159 30 17
MspA1I CmG'CkG
Cuts at: 1606 3373 3629 4577 4829 5100 5777 6925 7170
Size: 1767 256 948 252 271 677 1148 245
Cuts at: 7170 1606
Size: 2120
Fragments arranged by size:
2120 1767 1148 948 677 271 256 252
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4563 513
Size: 552 939 2559 3634
Fragments arranged by size:
3634 2559 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7684
NheI G'CTAG_C
Cuts at: 1052 3220 1052
Size: 2168 5516
NotI GC'GGCC_GC
Cuts at: 5131 5131
Size: 7684
NsiI A_TGCA'T
Cuts at: 4153 4153
Size: 7684
NspI r_CATG'y
Cuts at: 1132 1229 2706 2988 3172 4510 5042 6587 1132
Size: 97 1477 282 184 1338 532 1545 2229
Fragments arranged by size:
2229 1545 1477 1338 532 282 184 97
PasI CC'CwG_GG
Cuts at: 1348 4699 1348
Size: 3351 4333
PciI A'CATG_T
Cuts at: 2702 4506 6583 2702
Size: 1804 2077 3803
Fragments arranged by size:
3803 2077 1804
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2162 2166 2233 1108
Size: 1054 4 67 6559
Fragments arranged by size:
6559 1054 67 4
PfoI T'CCnGG_A
Cuts at: 2341 2341
Size: 7684
PmeI GTTT'AAAC
Cuts at: 4988 4988
Size: 7684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5753 5785 7266 7298 5753
Size: 32 1481 32 6139
Fragments arranged by size:
6139 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3835 4261 3835
Size: 426 7258
PshAI GACnn'nnGTC
Cuts at: 1613 2533 2705 1613
Size: 920 172 6592
Fragments arranged by size:
6592 920 172
PsiI TTA'TAA
Cuts at: 4526 5254 4526
Size: 728 6956
PstI C_TGCA'G
Cuts at: 830 2458 2756 3070 3372 3935 5036 830
Size: 1628 298 314 302 563 1101 3478
Fragments arranged by size:
3478 1628 1101 563 314 302 298
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7684
PvuII CAG'CTG
Cuts at: 3629 4577 4829 3629
Size: 948 252 6484
Fragments arranged by size:
6484 948 252
SalI G'TCGA_C
Cuts at: 1936 5026 1936
Size: 3090 4594
SapI GCTCTTCn'nnn_
Cuts at: 3765 3765
Size: 7684
SbfI CC_TGCA'GG
Cuts at: 5036 5036
Size: 7684
ScaI AGT'ACT
Cuts at: 2103 2349 3366 5932 7644 2103
Size: 246 1017 2566 1712 2143
Fragments arranged by size:
2566 2143 1712 1017 246
SexAI A'CCwGG_T
Cuts at: 2217 2217
Size: 7684
SfcI C'TryA_G
Cuts at: 826 1045 2454 2752 3066 3097 3368 3618 3624
Size: 219 1409 298 314 31 271 250 6
Cuts at: 3624 3675 3931 5032 6167 6848 7039 826
Size: 51 256 1101 1135 681 191 1471
Fragments arranged by size:
1471 1409 1135 1101 681 314 298 271
256 250 219 191 51 31 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7684
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7684
SmaI CCC'GGG
Cuts at: 1872 5011 1872
Size: 3139 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2868 3274 5788 6689 6951 820
Size: 197 926 925 406 2514 901 262 1553
Fragments arranged by size:
2514 1553 926 925 901 406 262 197
SnaBI TAC'GTA
Cuts at: 493 5475 493
Size: 4982 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7684
SphI G_CATG'C
Cuts at: 1132 2988 5042 1132
Size: 1856 2054 3774
Fragments arranged by size:
3774 2054 1856
SspI AAT'ATT
Cuts at: 5 52 3105 4279 5608 5
Size: 47 3053 1174 1329 2081
Fragments arranged by size:
3053 2081 1329 1174 47
StuI AGG'CCT
Cuts at: 1517 2995 1517
Size: 1478 6206
StyI C'CwwG_G
Cuts at: 513 1065 2004 2473 2482 3864 4563 5123 513
Size: 552 939 469 9 1382 699 560 3074
Fragments arranged by size:
3074 1382 939 699 560 552 469 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2154 2173 4576 5711 5728 5881 6066
Size: 1255 574 19 2403 1135 17 153 185
Cuts at: 6066 6511 325
Size: 445 1498
Fragments arranged by size:
2403 1498 1255 1135 574 445 185 153
19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2101 2347 2379
Size: 274 80 33 51 1443 124 246 32
Cuts at: 2379 2604 3364 5930 7642 96
Size: 225 760 2566 1712 138
Fragments arranged by size:
2566 1712 1443 760 274 246 225 138
124 80 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2980 3012 7644 7676 2980
Size: 32 4632 32 2988
Fragments arranged by size:
4632 2988 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7684
XbaI T'CTAG_A
Cuts at: 4657 5020 4657
Size: 363 7321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2163 2480 3046 2163
Size: 317 566 6801
Fragments arranged by size:
6801 566 317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7684
XmaI C'CCGG_G
Cuts at: 1870 5009 1870
Size: 3139 4545
XmnI GAAnn'nnTTC
Cuts at: 3141 5813 3141
Size: 2672 5012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5492 276
Size: 53 83 186 936 3958 2468
Fragments arranged by size:
3958 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI