Restriction map for
pFN21AB4123
(Circular) (Six-base) MAPSORT of: pFN21AB4123.seq Check: 8604 from: 1 to: 6109
pFN21AB4123 6109 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2886 3452 1937
Size: 949 566 4594
Fragments arranged by size:
4594 949 566
Acc65I G'GTAC_C
Cuts at: 3216 3357 3430 3216
Size: 141 73 5895
Fragments arranged by size:
5895 141 73
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 4172 5556 5824 1632
Size: 447 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 447 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 2664 5008 5985 1203
Size: 1461 2344 977 1327
Fragments arranged by size:
2344 1461 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 2570 2683 2685 1166
Size: 1404 113 2 4590
Fragments arranged by size:
4590 1404 113 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 3852 5424 3852
Size: 1572 4537
ApaI G_GGCC'C
Cuts at: 3303 3303
Size: 6109
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 3116 3418 3644 3796 5905 1493
Size: 1623 302 226 152 2109 1697
Fragments arranged by size:
2109 1697 1623 302 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 2199 3434 1870
Size: 73 199 57 1235 4545
Fragments arranged by size:
4545 1235 199 73 57
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2237 3216 3357 3430
Size: 325 217 627 326 124 979 141 73
Cuts at: 3430 4885 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 979 627 326 325 217 141
124 73
BanII G_rGCy'C
Cuts at: 1391 2718 3058 3303 3428 1391
Size: 1327 340 245 125 4072
Fragments arranged by size:
4072 1327 340 245 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2648 3402 928
Size: 971 749 754 3635
Fragments arranged by size:
3635 971 754 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2999 3033 4300 4334 1903
Size: 34 1062 34 1267 34 3678
Fragments arranged by size:
3678 1267 1062 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2512 4001 5211 2512
Size: 1489 1210 3410
Fragments arranged by size:
3410 1489 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BfrBI ATG'CAT
Cuts at: 2895 2895
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 4720 136
Size: 107 122 71 631 605 362 2686 1525
Fragments arranged by size:
2686 1525 631 605 362 122 107 71
BglII A'GATC_T
Cuts at: 2580 6104 2580
Size: 3524 2585
BlpI GC'TnA_GC
Cuts at: 2513 3526 2513
Size: 1013 5096
Bme1580I G_kGCm'C
Cuts at: 1790 3303 4170 5326 1790
Size: 1513 867 1156 2573
Fragments arranged by size:
2573 1513 1156 867
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2008 3072 4769 1239
Size: 769 1064 1697 2579
Fragments arranged by size:
2579 1697 1064 769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 4234 5099 5397 2792
Size: 1442 865 298 3504
Fragments arranged by size:
3504 1442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2866 4772 882
Size: 617 1367 1906 2219
Fragments arranged by size:
2219 1906 1367 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 2923 6103 2923
Size: 3180 2929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2733 3213 4542 4940 5214 5361 5772 2733
Size: 480 1329 398 274 147 411 3070
Fragments arranged by size:
3070 1329 480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5884
Fragments arranged by size:
5884 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2575 1186
Size: 1389 4720
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3395 1163
Size: 573 1659 3877
Fragments arranged by size:
3877 1659 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2741 4170 4255 5326 2741
Size: 1429 85 1071 3524
Fragments arranged by size:
3524 1429 1071 85
BsmI GAATG_Cn'
Cuts at: 2895 3277 3620 3713 2895
Size: 382 343 93 5291
Fragments arranged by size:
5291 382 343 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2718 2741 3058 3303 3428 4170 4255
Size: 399 928 23 317 245 125 742 85
Cuts at: 4255 5326 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 928 742 399 317 245 125
85 23
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 2374 3267 3998 5728 2374
Size: 893 731 1730 2755
Fragments arranged by size:
2755 1730 893 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 3464 844
Size: 1664 956 3489
Fragments arranged by size:
3489 1664 956
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2406 96
Size: 2310 3799
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3352 794
Size: 2558 3551
BstBI TT'CG_AA
Cuts at: 2596 4913 2596
Size: 2317 3792
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2580 3439 3806 4191 4208 5660 5746 5758
Size: 608 859 367 385 17 1452 86 12
Cuts at: 5758 6104 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 859 608 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3321 3321
Size: 6109
BtgI C'CryG_G
Cuts at: 513 1065 2004 2877 513
Size: 552 939 873 3745
Fragments arranged by size:
3745 939 873 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2704 487
Size: 683 56 1478 3892
Fragments arranged by size:
3892 1478 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 4419 4439 3625
Size: 794 20 5295
Fragments arranged by size:
5295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DraIII CAC_nnn'GTG
Cuts at: 2347 2347
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3556 4445 6074
Size: 54 1425 50 275 18 1726 889 1629
Cuts at: 6074 8
Size: 43
Fragments arranged by size:
1726 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2170 2330 2351 4039 1353
Size: 817 160 21 1688 3423
Fragments arranged by size:
3423 1688 817 160 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2225 4696 5070 5216 387
Size: 1064 138 636 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 636 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2079 3072 4172 4769 5556 5824
Size: 393 376 71 993 1100 597 787 268
Cuts at: 5824 1239
Size: 1524
Fragments arranged by size:
1524 1100 993 787 597 393 376 268
71
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 3236
Size: 6109
EcoO109I rG'GnC_Cy
Cuts at: 3315 3315
Size: 6109
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 5256 1678
Size: 3578 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2362 2394 4750
Size: 32 135 32 610 32 148 32 2356
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2356 610 148 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3220 3361 3434 3220
Size: 141 73 5895
Fragments arranged by size:
5895 141 73
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5223 5407 1305
Size: 3918 184 2007
Fragments arranged by size:
3918 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 2683 2685 3308 4067 4426 4585
Size: 648 1404 113 2 623 759 359 159
Cuts at: 4585 518
Size: 2042
Fragments arranged by size:
2042 1404 759 648 623 359 159 113
2
MspA1I CmG'CkG
Cuts at: 1606 2263 2570 3525 4202 5350 5595 1606
Size: 657 307 955 677 1148 245 2120
Fragments arranged by size:
2120 1148 955 677 657 307 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2877 513
Size: 552 939 873 3745
Fragments arranged by size:
3745 939 873 552
NdeI CA'TA_TG
Cuts at: 387 2891 3100 387
Size: 2504 209 3396
Fragments arranged by size:
3396 2504 209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NsiI A_TGCA'T
Cuts at: 2897 2897
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspOMI G'GGCC_C
Cuts at: 3299 3299
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SapI GCTCTTCn'nnn_
Cuts at: 2330 2330
Size: 6109
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2317 3457 4592 5273 5464 826
Size: 219 1272 1140 1135 681 191 1471
Fragments arranged by size:
1471 1272 1140 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 4213 5114 5376 820
Size: 197 926 864 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 864 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 2168 2693 4033 5
Size: 47 2116 525 1340 2081
Fragments arranged by size:
2116 2081 1340 525 47
StuI AGG'CCT
Cuts at: 1517 2934 1517
Size: 1417 4692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2725 2877 3548 513
Size: 552 939 721 152 671 3074
Fragments arranged by size:
3074 939 721 671 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 4136 4153 4306 4491 4936 325
Size: 1255 759 1797 17 153 185 445 1498
Fragments arranged by size:
1797 1498 1255 759 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 2430 2826
Size: 274 80 33 51 1443 429 24 396
Cuts at: 2826 4355 6067 96
Size: 1529 1712 138
Fragments arranged by size:
1712 1529 1443 429 396 274 138 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6069 6101 6069
Size: 32 6077
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI