Restriction map for
pFN21AB4121
(Circular) (Six-base) MAPSORT of: pFN21AB4121.seq Check: 9073 from: 1 to: 6514
pFN21AB4121 6514 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2072 4324 278
Size: 53 83 186 936 536 2252 2468
Fragments arranged by size:
2468 2252 936 536 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 3857 1937
Size: 1095 825 4594
Fragments arranged by size:
4594 1095 825
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 4641 5014 1563
Size: 3078 373 3063
Fragments arranged by size:
3078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3084 4577 5961 6229 1632
Size: 1452 1493 1384 268 1917
Fragments arranged by size:
1917 1493 1452 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6317
AflIII A'CryG_T
Cuts at: 1203 2424 5413 6390 1203
Size: 1221 2989 977 1327
Fragments arranged by size:
2989 1327 1221 977
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6514
AlwNI CAG_nnn'CTG
Cuts at: 4257 5829 4257
Size: 1572 4942
ApaLI G'TGCA_C
Cuts at: 4571 5727 4571
Size: 1156 5358
ApoI r'AATT_y
Cuts at: 1493 2317 2676 2814 3254 3374 3527 3823 4049
Size: 824 359 138 440 120 153 296 226
Cuts at: 4049 4201 6310 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 824 440 359 296 226 153
152 138 120
AseI AT'TA_AT
Cuts at: 160 1032 2568 5068 160
Size: 872 1536 2500 1606
Fragments arranged by size:
2500 1606 1536 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2275 3459 3839 1870
Size: 73 130 202 1184 380 4545
Fragments arranged by size:
4545 1184 380 202 130 73
BamHI G'GATC_C
Cuts at: 3844 4211 3844
Size: 367 6147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3835 5290 618
Size: 325 217 627 2048 1455 1842
Fragments arranged by size:
2048 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2586 3684 3833 1391
Size: 1195 1098 149 4072
Fragments arranged by size:
4072 1195 1098 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4705 4739 1903
Size: 34 2768 34 3678
Fragments arranged by size:
3678 2768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4406 5616 4406
Size: 1210 5304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6514
BfrBI ATG'CAT
Cuts at: 2903 2903
Size: 6514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2870 5125 136
Size: 107 122 71 631 605 1198 2255 1525
Fragments arranged by size:
2255 1525 1198 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2092 6509 2092
Size: 4417 2097
BlpI GC'TnA_GC
Cuts at: 3153 3931 3153
Size: 778 5736
Bme1580I G_kGCm'C
Cuts at: 1790 4575 5731 1790
Size: 2785 1156 2573
Fragments arranged by size:
2785 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4245 5203 442
Size: 416 53 83 399 24 2828 958 1753
Fragments arranged by size:
2828 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2088 2088
Size: 6514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3305 5174 1239
Size: 2066 1869 2579
Fragments arranged by size:
2579 2066 1869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3737 4639 5504 5802 3737
Size: 902 865 298 4449
Fragments arranged by size:
4449 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5177 882
Size: 617 3678 2219
Fragments arranged by size:
3678 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4305 493
Size: 711 3101 2702
Fragments arranged by size:
3101 2702 711
BsaBI GATnn'nnATC
Cuts at: 2904 6508 2904
Size: 3604 2910
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 4321 4703 5338
Size: 53 83 186 936 536 2252 382 635
Cuts at: 5338 275
Size: 1451
Fragments arranged by size:
2252 1451 936 635 536 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2643 4947 5345 5619 5766 6177 2643
Size: 2304 398 274 147 411 2980
Fragments arranged by size:
2980 2304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 1178
Size: 30 165 30 636 30 5623
Fragments arranged by size:
5623 636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 2895 1186
Size: 1139 570 4805
Fragments arranged by size:
4805 1139 570
BseYI C'CCAG_C
Cuts at: 2846 5717 6213 2846
Size: 2871 496 3147
Fragments arranged by size:
3147 2871 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5941
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3964 4725 5753 1540
Size: 275 186 1963 761 1028 2301
Fragments arranged by size:
2301 1963 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 3684 4575 4660 5731 2586
Size: 1098 891 85 1071 3369
Fragments arranged by size:
3369 1098 1071 891 85
BsmI GAATG_Cn'
Cuts at: 4025 4118 4025
Size: 93 6421
BsmBI CGTCTCn'nnnn_
Cuts at: 2076 2076
Size: 6514
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2586 3684 3833 4575 4660 5731 1391
Size: 399 796 1098 149 742 85 1071 2174
Fragments arranged by size:
2174 1098 1071 796 742 399 149 85
BspEI T'CCGG_A
Cuts at: 2643 6177 2643
Size: 3534 2980
BspHI T'CATG_A
Cuts at: 4403 6133 4403
Size: 1730 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3869 844
Size: 3025 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4401 1394
Size: 429 2578 3507
Fragments arranged by size:
3507 2578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3124 5009 5183 65
Size: 1183 1876 1885 174 1396
Fragments arranged by size:
1885 1876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5158 5345 1700
Size: 110 3348 187 2869
Fragments arranged by size:
3348 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6514
BssSI C'ACGA_G
Cuts at: 2416 4574 5586 2416
Size: 2158 1012 3344
Fragments arranged by size:
3344 2158 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 3790 794
Size: 1742 1254 3518
Fragments arranged by size:
3518 1742 1254
BstBI TT'CG_AA
Cuts at: 3213 5318 3213
Size: 2105 4409
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstYI r'GATC_y
Cuts at: 1972 2092 2409 3844 4211 4596 4613 6065 6151
Size: 120 317 1435 367 385 17 1452 86
Cuts at: 6151 6163 6509 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1435 385 367 346 317 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2360 2360
Size: 6514
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5023
Fragments arranged by size:
5023 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5775
Fragments arranged by size:
5775 683 56
BtsI GCAGTG_nn'
Cuts at: 2865 3136 4030 4824 4844 2865
Size: 271 894 794 20 4535
Fragments arranged by size:
4535 894 794 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6479
DraI TTT'AAA
Cuts at: 2466 2967 3818 2466
Size: 501 851 5162
Fragments arranged by size:
5162 851 501
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5521 809
Size: 507 4205 1802
Fragments arranged by size:
4205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2862 3168 3961
Size: 54 1425 50 275 18 1032 306 793
Cuts at: 3961 4850 6479 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 793 306 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3961 1537
Size: 275 2149 4090
Fragments arranged by size:
4090 2149 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2389 3101 4444 1353
Size: 829 207 712 1343 3423
Fragments arranged by size:
3423 1343 829 712 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5101 5475 5621 387
Size: 1064 138 3512 374 146 1280
Fragments arranged by size:
3512 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2584 3682 2584
Size: 1098 5416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3084 3305 4577 5174 5961 6229 1239
Size: 393 1452 221 1272 597 787 268 1524
Fragments arranged by size:
1524 1452 1272 787 597 393 268 221
EcoO109I rG'GnC_Cy
Cuts at: 2664 2664
Size: 6514
EcoRI G'AATT_C
Cuts at: 2676 3374 3823 4201 2676
Size: 698 449 378 4989
Fragments arranged by size:
4989 698 449 378
FspI TGC'GCA
Cuts at: 5020 5020
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 5661 1678
Size: 3983 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2067 2071 2099 2181
Size: 32 135 32 467 28 4 28 82
Cuts at: 2181 2213 5155 5187 5229 5261 1373
Size: 32 2942 32 42 32 2626
Fragments arranged by size:
2942 2626 467 135 82 42 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3858 4104 669
Size: 988 281 1920 246 3079
Fragments arranged by size:
3079 1920 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3746 748
Size: 2998 3516
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 4113 4113
Size: 6514
MluI A'CGCG_T
Cuts at: 2424 6390 2424
Size: 3966 2548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3162 5628 5812 1305
Size: 1857 2466 184 2007
Fragments arranged by size:
2466 2007 1857 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2864 3170 10
Size: 54 1425 343 1032 306 3354
Fragments arranged by size:
3354 1425 1032 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3129 3176 3294 4472 4831 4990 518
Size: 648 1963 47 118 1178 359 159 2042
Fragments arranged by size:
2042 1963 1178 648 359 159 118 47
MspA1I CmG'CkG
Cuts at: 1606 3157 3930 4607 5755 6000 1606
Size: 1551 773 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 773 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5023
Fragments arranged by size:
5023 939 552
NdeI CA'TA_TG
Cuts at: 387 2159 387
Size: 1772 4742
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NsiI A_TGCA'T
Cuts at: 2905 2905
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 3872 5417 1132
Size: 97 2643 1545 2229
Fragments arranged by size:
2643 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6514
PciI A'CATG_T
Cuts at: 5413 5413
Size: 6514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6514
PfoI T'CCnGG_A
Cuts at: 2119 2119
Size: 6514
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3714 3746 4583 4615 6096 6128 3714
Size: 32 837 32 1481 32 4100
Fragments arranged by size:
4100 1481 837 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 2664
Size: 6514
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 3398 4084 3398
Size: 686 5828
PstI C_TGCA'G
Cuts at: 830 3729 3866 830
Size: 2899 137 3478
Fragments arranged by size:
3478 2899 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 3157 3157
Size: 6514
SacI G_AGCT'C
Cuts at: 2586 3684 2586
Size: 1098 5416
SalI G'TCGA_C
Cuts at: 1936 3856 1936
Size: 1920 4594
SbfI CC_TGCA'GG
Cuts at: 3866 3866
Size: 6514
ScaI AGT'ACT
Cuts at: 4762 6474 4762
Size: 1712 4802
SfcI C'TryA_G
Cuts at: 826 1045 3193 3363 3725 3862 4997 5678 5869
Size: 219 2148 170 362 137 1135 681 191
Cuts at: 5869 826
Size: 1471
Fragments arranged by size:
2148 1471 1135 681 362 219 191 170
137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6514
SmaI CCC'GGG
Cuts at: 1872 3841 1872
Size: 1969 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 2275 3459 3716 4618 5519
Size: 197 926 130 202 1184 257 902 901
Cuts at: 5519 5781 820
Size: 262 1553
Fragments arranged by size:
1553 1184 926 902 901 262 257 202
197 130
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SspI AAT'ATT
Cuts at: 5 52 2944 4438 5
Size: 47 2892 1494 2081
Fragments arranged by size:
2892 2081 1494 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6514
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 3165 3953 513
Size: 552 939 39 1122 788 3074
Fragments arranged by size:
3074 1122 939 788 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 3595 4541 4558 4711 4896 5341
Size: 1255 675 1340 946 17 153 185 445
Cuts at: 5341 325
Size: 1498
Fragments arranged by size:
1498 1340 1255 946 675 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4760 6472 96
Size: 274 80 33 51 1443 2783 1712 138
Fragments arranged by size:
2783 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6474 6506 6474
Size: 32 6482
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6514
XbaI T'CTAG_A
Cuts at: 2329 3850 2329
Size: 1521 4993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2688 3178 2688
Size: 490 6024
XhoI C'TCGA_G
Cuts at: 1943 2073 2275 3459 1943
Size: 130 202 1184 4998
Fragments arranged by size:
4998 1184 202 130
XmaI C'CCGG_G
Cuts at: 1870 3839 1870
Size: 1969 4545
XmnI GAAnn'nnTTC
Cuts at: 4643 4643
Size: 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2070 4322 276
Size: 53 83 186 936 536 2252 2468
Fragments arranged by size:
2468 2252 936 536 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI